BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	356,677	2 bp→CC	intergenic (+172/+164)	codA → / ← cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	356,677	0	T	C	97.7%	704.3 / NA	302	intergenic (+172/+165)	codA/cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base T (6/1); new base C (116/178); total (122/180)
Fisher's exact test for biased strand distribution p-value = 1.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	356,678	0	A	C	98.3%	706.4 / NA	302	intergenic (+173/+164)	codA/cynR	cytosine/isoguanine deaminase/transcriptional regulator CynR
Reads supporting (aligned to +/- strand): ref base A (3/2); new base C (117/176); total (122/180)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

TGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATC  >  NZ_CP025268/356548‑356809

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tGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGcc                                                                                                                            <  1:3483147/140‑1 (MQ=11)

agAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCATGATAAGCGTAGCGCAGCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAGCCGCGCATTCCTCCAACGcc                                                                                                                            <  2:2182842/139‑1 (MQ=11)

               gCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCAGCAGGCGATTCCAGCCGCCGATCTGTGTCTGCGGCTACCGTGATTCATTCCCGCCAACAACCTCGCATTTCTCCAACGCCATGTGCAAAAATGcc                                                                                                             >  2:3886819/1‑140 (MQ=11)

                ccTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCt                                                                                                            >  2:1214567/1‑140 (MQ=255)

                 cTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCTTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCtt                                                                                                           <  1:1229135/140‑1 (MQ=11)

                     gTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCa                                                                                                       <  2:2002474/140‑1 (MQ=11)

                      tAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTCCTCCTCCGCCATGTGCAAAAATGCCTTCGCAg                                                                                                      <  1:2413720/140‑1 (MQ=11)

                       agagCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGc                                                                                                     <  1:3655222/140‑1 (MQ=255)

                        gagCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATTCCTTCGCAGCg                                                                                                    <  1:4221272/140‑1 (MQ=11)

                         agCACTGAACGCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCgg                                                                                                   <  1:4412350/140‑1 (MQ=11)

                          gCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGGCAGCGGCTACAGTGATGCAGTCCCGCCAACAACCGCGCATGCCTCCAACGCCATGTGCAAAAATGCCTTCGCggcggc                                                                                                  >  2:3203180/1‑140 (MQ=11)

                           cACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCt                                                                                                 >  1:2730567/1‑140 (MQ=255)

                              tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                                                              >  1:993644/1‑140 (MQ=11)

                              tGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTc                                                                                              >  1:4332800/1‑140 (MQ=14)

                                aaCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTg                                                                                            >  1:1860983/1‑140 (MQ=255)

                                 aatCGTAGGCAGGATAGGCGTAGCGCATCAGGGAATTCCAGCCGCTGATCTGTGTAGGCGGCTACCGTGATTCGTTCCCGCCAACAACCGCGCATGCCTCCAACGACATGTGCAAAAATGCCATCGCAGCGGCTGTCTga                                                                                           <  2:185661/138‑2 (MQ=11)

                                  cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGcc                                                                                          <  1:3982307/140‑1 (MQ=14)

                                  cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGcc                                                                                          <  1:2239776/140‑1 (MQ=14)

                                   tCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCa                                                                                         >  2:3548532/1‑140 (MQ=14)

                                    cGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAg                                                                                        <  1:2934951/140‑1 (MQ=14)

                                     gTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGc                                                                                       >  1:59017/1‑140 (MQ=255)

                                      tAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCt                                                                                      >  1:4998359/1‑140 (MQ=11)

                                       aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTg                                                                                     <  2:1361519/140‑1 (MQ=14)

                                          ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAg                                                                                  >  1:4733169/1‑140 (MQ=14)

                                             gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCCTTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGttt                                                                               >  1:2162530/1‑140 (MQ=14)

                                                   cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCg                                                                         >  1:4294253/1‑140 (MQ=17)

                                                   cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCg                                                                         >  1:2142791/1‑140 (MQ=17)

                                                   cGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCAGTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCg                                                                         >  1:512992/1‑140 (MQ=17)

                                                     tAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGa                                                                       >  2:2778515/1‑140 (MQ=17)

                                                        cgcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgc                                                                    >  1:4207338/1‑140 (MQ=17)

                                                        cgcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAAAAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgc                                                                    >  1:728472/1‑140 (MQ=17)

                                                         gcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcg                                                                   <  1:3262346/140‑1 (MQ=17)

                                                         gcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGAAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcg                                                                   <  1:3072107/140‑1 (MQ=17)

                                                            tCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcggcg                                                                >  1:2690540/1‑140 (MQ=18)

                                                             cAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATgcggcgg                                                               >  2:3043136/1‑139 (MQ=17)

                                                             cAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTCGTTTAGGCCGGATgcggcgg                                                               >  2:381478/1‑139 (MQ=17)

                                                             cAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGt                                                               >  2:2117191/1‑140 (MQ=18)

                                                             cAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGt                                                               >  2:4076023/1‑140 (MQ=18)

                                                             cAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCAGATGCGGCGt                                                               >  1:1116094/1‑140 (MQ=17)

                                                             cAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATCCCGGATGCGGCGt                                                               >  1:4473723/1‑140 (MQ=17)

                                                              aGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTCATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTg                                                              <  2:3122470/140‑1 (MQ=18)

                                                               ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                             <  1:4030342/140‑1 (MQ=18)

                                                               ggCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGa                                                             <  1:4860447/140‑1 (MQ=18)

                                                                   aTTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                                                         <  2:4716205/140‑1 (MQ=255)

                                                                   aTTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGc                                                         <  1:1654881/140‑1 (MQ=18)

                                                                    ttCCAGCCGCTGATCTGTGGCAGCGGCAACCGTGAATCATTCCCGCCAGCCACCGCGCCTTCCTCCAACGCCATGTGCACAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTTGTTTATGCCCGCTGCGTCGTCTACGcc                                                        >  2:1038359/1‑140 (MQ=255)

                                                                        aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACACCCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                    <  1:3502108/140‑1 (MQ=21)

                                                                        aGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTAt                                                    <  1:1446359/140‑1 (MQ=21)

                                                                         gCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATc                                                   >  1:2908300/1‑140 (MQ=21)

                                                                         gCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTATTGTTTATCCCCGATAAGGCCTGAGCGCCTTATc                                                   >  2:1084658/1‑140 (MQ=14)

                                                                            gCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                <  1:2089959/140‑1 (MQ=21)

                                                                             ccgATCCGTGTCGGCGGCTACCGCGCTTCATTCCCTCCAACAACCGCGCATTCCTCCAACGCCATCTGCAAAAATGCCTTCGCAGCGGCTGTCTCCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCTGc                                               <  2:486722/138‑1 (MQ=37)

                                                                             cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                               <  1:1059054/140‑1 (MQ=21)

                                                                             cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                               <  1:3876071/140‑1 (MQ=21)

                                                                             cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                               <  1:3156974/140‑1 (MQ=21)

                                                                             cTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                               <  1:3611721/140‑1 (MQ=21)

                                                                             cTGATCTGTGTCACCGGCTACCACGCTTCACTCCCGCCAACAACCGCGCATTCCTCCGACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCCTCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGc                                               <  2:2497975/140‑1 (MQ=21)

                                                                              tGATCTGGGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCCCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATAAGGCGTTCACGCCGCATCCGGca                                              <  2:1191738/140‑2 (MQ=17)

                                                                               gATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCt                                             <  1:4377634/140‑1 (MQ=255)

                                                                                  cTGTGTCAGCTGCTACCGTGATTCATTCCCACCAACATCCCCGCATTCCTCCCACGCCATGTGCAACAATGTCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg                                          <  2:3994081/140‑1 (MQ=37)

                                                                                  cTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAATAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg                                          <  2:57688/140‑1 (MQ=21)

                                                                                  cTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCCGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACg                                          <  1:234788/140‑1 (MQ=21)

                                                                                    gtgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGTCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                        <  1:1025521/140‑1 (MQ=21)

                                                                                    gtgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                        <  1:3012408/140‑1 (MQ=21)

                                                                                    gtgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                        <  1:3832171/140‑1 (MQ=21)

                                                                                    gtgtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCCCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTa                                        <  2:4981509/140‑1 (MQ=21)

                                                                                    gtgtCAGCGGCTACCGCGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGCGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACgca                                        <  1:4076437/140‑3 (MQ=21)

                                                                                       tCAGCGGCTACCGTGATTCATTACCGCCAACATCCGCGCATCACCCCGACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTagag                                     <  2:3603625/140‑1 (MQ=21)

                                                                                         aGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCa                                   <  1:916862/140‑1 (MQ=25)

                                                                                          gCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCAc                                  >  2:1701387/1‑140 (MQ=25)

                                                                                          gCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGAATGCGACGGGAACGCCTTATCCGGCCTACGTAGAGctc                                  >  1:2003101/1‑138 (MQ=21)

                                                                                          gCGGCTACCGGGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCAATGTGCAAAAATGCCGTGGCAGCGGCTGTCTGCCAGCTGTAGTTCATGCCCCATGCAGCGGGCACGCACTATCCGGTCTGCGTAGCGCAc                                  >  2:4578033/1‑140 (MQ=12)

                                                                                           cGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTCATGCCGGATGCGCCGGCAACGCCTTATCGGGCCCACGTAGAGCACt                                 >  2:2068860/1‑140 (MQ=18)

                                                                                             gctACCGTGATTCATTCCCGACAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCTGCTGTCTTCCAGCTGTAGTTTATGCCGTATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGa                               <  2:68418/138‑1 (MQ=25)

                                                                                               tACCGTGATTCATTCCCGCCAACAACCGCGCATTCTTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTACGTAGAGGACTGAAc                             >  1:1727107/1‑140 (MQ=25)

                                                                                               tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAAc                             >  1:5095033/1‑140 (MQ=255)

                                                                                               tACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCAGCGTGCACGCCCTCTCCGGCCTGCGTAGAGCACTGAAc                             >  2:3300780/1‑140 (MQ=21)

                                                                                                aCCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGACGCGGCGTCCACGCCCTATCCTGCCTACGTAGAGCACTGAACt                            >  2:4798852/1‑140 (MQ=21)

                                                                                                  cgcgATTCATTCTCGCCAACAGCCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCg                          <  2:138627/137‑1 (MQ=25)

                                                                                                  cGTTATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATACCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCg                          <  2:874735/140‑1 (MQ=25)

                                                                                                  cGTGATTCATTCCCGCCAAGAAACGGGCATTTCTCCAATGGCATGTGGAAGCGTGGCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCTTACGTAGTGCACTGAACTCg                          <  2:2558446/140‑1 (MQ=21)

                                                                                                       ttCATTCCCGCCAACATCCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                     <  2:4161555/140‑1 (MQ=255)

                                                                                                       ttCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                     <  1:4581938/140‑1 (MQ=255)

                                                                                                       ttCATCCCCGCCGCCAACCGCGCCTGCCTCCAATGCGTTGTGCAAAAATGGTTGCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                     <  2:2355464/140‑1 (MQ=18)

                                                                                                       ttCATCCCCGCCAACACGCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGc                     <  2:4478936/140‑1 (MQ=25)

                                                                                                         cATTCCCGCCAACTACCGCGCATTCCTCCAACGCCATGTGCAAAGATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCt                   <  2:1550389/140‑1 (MQ=25)

                                                                                                          acacccGCCAACGTCCGTGCAGTCCTCCAACGCCATGTACAAAAATGCATTCGCAGCGGCTGTCTGCCCGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTg                  <  2:3454905/136‑1 (MQ=18)

                                                                                                           ctcccGCCAGCATCCGCGCAGCCCTCCTACGCCATGTGCAACAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGa                 <  2:2255754/137‑1 (MQ=21)

                                                                                                            tcccGCCAACAACCGCGCATTCCTCCGACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                <  2:3522161/138‑1 (MQ=25)

                                                                                                            tcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                >  1:2022409/3‑140 (MQ=255)

                                                                                                            tcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                <  1:3548532/138‑1 (MQ=255)

                                                                                                            tcccGCCAACAACCGCGCATTCCTCCAACGCCATGTGAAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                <  1:651265/138‑1 (MQ=255)

                                                                                                            acccGCCAACAACCGCGGATTCCTCCGACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGAt                <  2:2996655/138‑1 (MQ=25)

                                                                                                                gCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGc            >  1:4806627/1‑140 (MQ=255)

                                                                                                                        ctccgcATTCTTCCACCGCCATGTGCCACAATTCCTTCACAGCGGCTGTCCGGCAGCTGTAGTTTATGCCGGATGCTGCGTGAACGCCTTATCCGGCCTATGTAGAGCACTGAACTCGTAGGCGTGCTAAGCGTAGCGCa    <  2:3171413/137‑1 (MQ=17)

                                                                                                                        ccGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCGCTGAACTCGTAGGCCTGATAAGCGTAGCGCa    <  1:3865862/140‑1 (MQ=255)

                                                                                                                        ccGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCa    <  2:4332800/140‑1 (MQ=255)

                                                                                                                        ccGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCa    <  1:1306181/140‑1 (MQ=255)

                                                                                                                        ccGCGCATCCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCa    <  1:2501575/140‑1 (MQ=255)

                                                                                                                         cgcgCATTCCTCCGACGCCATGGGCTACAATGGCTTCGCAGCGGCGGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACATAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCAt   <  2:2889018/140‑1 (MQ=18)

||

TGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGTTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTAGTTTATGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGTAGAGCACTGAACTCGTAGGCCTGATAAGCGTAGCGCATC  >  NZ_CP025268/356548‑356809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`