BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,728,808	(C)_10→8	pseudogene (1254‑1255/1457 nt)	xylB ←	xylulokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,728,800	0	C	.	89.1%	511.0 / 57.2	139	pseudogene (1263/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (14/1); new base . (46/77); total (61/78)
Fisher's exact test for biased strand distribution p-value = 3.23e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	3,728,801	0	C	.	90.4%	423.6 / 70.5	139	pseudogene (1262/1457 nt)	xylB	xylulokinase
Reads supporting (aligned to +/- strand): ref base C (1/2); major base . (38/75); minor base G (12/0); total (61/78)
Fisher's exact test for biased strand distribution p-value = 6.89e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

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ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAAGTCAATGAGCGATTTCACTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                       <  2:10503853/140‑1 (MQ=255)

   tGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccc                                                                                                                                    <  1:933413/140‑1 (MQ=255)

        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  1:7435646/140‑2 (MQ=255)

        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  2:5963266/140‑2 (MQ=255)

        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  1:9488364/140‑2 (MQ=255)

        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                               <  2:4505897/140‑2 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                             <  1:6372232/140‑4 (MQ=255)

          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgta                                                                                                                             <  2:3140094/140‑4 (MQ=255)

            gCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtacg                                                                                                                           >  2:2408083/1‑135 (MQ=255)

                  cGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATc                                                                                                                   <  1:10404097/140‑1 (MQ=255)

                   gCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCg                                                                                                                  <  1:492313/140‑1 (MQ=255)

                    cTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGa                                                                                                                 >  2:5641260/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAgct                                                                                                              >  2:4810379/1‑140 (MQ=255)

                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAgct                                                                                                              >  2:418662/1‑140 (MQ=255)

                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  1:10267540/140‑1 (MQ=255)

                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                           <  1:8731058/140‑1 (MQ=255)

                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgcggct                                                                                                           <  2:7433861/140‑1 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                         <  1:7751938/140‑1 (MQ=255)

                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑‑CCCCCCCGTACGGGAATCGAGCTGCTGAc                                                                                                        >  2:4028910/1‑140 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGGAATCGAGCTGCGGACGGCGGag                                                                                                 >  2:832687/1‑139 (MQ=255)

                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGat                                                                                                 <  1:9187958/140‑1 (MQ=255)

                                       gACTGTTCTAACGGTAGTTGCGTCAACACTTCAATGATCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCACGTACGGTAATCGAGCTGCTGACCGCTGatat                                                                                               <  1:7591452/140‑1 (MQ=255)

                                           gTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGcc                                                                                          <  1:2057794/140‑1 (MQ=255)

                                            ttCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTTCGGGGATCGAGCTGCTGAGGGCTGGTATCCGGCa                                                                                         >  2:127816/1‑140 (MQ=255)

                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                        <  1:7650979/140‑1 (MQ=255)

                                               tAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCa                                                                                      <  1:6280351/140‑1 (MQ=255)

                                                 aCGGTAGTTGCGGCAACAATTAAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CGCCCCCCGGACGGTACTCGAGCTGCTGACCGCTGATAACCGCCAGCATc                                                                                    >  2:10538887/1‑140 (MQ=255)

                                                  cGGTAGGTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  2:2688391/140‑1 (MQ=255)

                                                    gTAGTTGCTGCAACAATTCACTGAGCGCTGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGAATCTGa                                                                                 <  2:6329215/140‑1 (MQ=255)

                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                 <  2:4666991/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCGAGCATCTGACg                                                                               >  2:6464368/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               >  2:10298524/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  1:9873555/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  1:6823620/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                               <  2:9606688/140‑1 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCGCCGTACGGGCGTCGGGCTGCTGGGCGGGGATAGCCGCGAGCAGCTGACg                                                                               >  1:195294/1‑140 (MQ=255)

                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGACGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCACCACCGTACGGTATTAGAGCTGCTGACCGCTGATATCCGCGAGCATCTGACg                                                                               >  2:2385331/1‑140 (MQ=255)

                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                              <  2:9455971/140‑1 (MQ=255)

                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCGCCCCCCGTACGGTAATGGTGGTGCTGGCCGCGGTTTTGCGCCCGCAGCTGACGc                                                                              >  2:28914/1‑140 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                             <  2:10094238/140‑1 (MQ=255)

                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                             <  1:7684136/140‑1 (MQ=255)

                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGGGCTGCTGACCGGTGATGTCCGCCAGCATCGGACGcccg                                                                           >  1:4653780/1‑138 (MQ=255)

                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGGCGGGAGTGGTGCGGCGGACCGGGGGTGTCCGCCGGCGTCTGACGCCAg                                                                           >  1:4653581/1‑140 (MQ=255)

                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTTCGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCGTCTGACGCCAGTAc                                                                        >  2:8524930/1‑140 (MQ=255)

                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                       <  1:3619213/140‑1 (MQ=255)

                                                              caacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACt                                                                       <  1:5641260/140‑1 (MQ=255)

                                                               aacaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTc                                                                      <  2:10598255/140‑1 (MQ=255)

                                                                acaaTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCa                                                                     <  1:4879905/140‑1 (MQ=255)

                                                                   aTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTCATCGTGCTGCTGACCGCTGATGTCCGCCAGCAGCTGGCGCGGGTTCTCagtc                                                                  >  2:6914290/1‑137 (MQ=255)

                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCAGTACGGTAATCGAGCTTCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                 <  1:2385331/140‑1 (MQ=255)

                                                                          gAGCGATTTCTCTGGATTCGCCGCGCTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACTcgccc                                                           <  2:941856/140‑1 (MQ=255)

                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  1:1059360/140‑1 (MQ=255)

                                                                          gAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccc                                                           <  1:2358148/140‑1 (MQ=255)

                                                                           aGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTTCGGTAAGCGAGCTGCGGACCGCTGATATGCGCCAGCATCTGACGCCAGTACTCACTACGcgcccc                                                          >  2:1940446/1‑140 (MQ=255)

                                                                            gCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGGAATCGTGCTGCTGACCGCTGAGATCCGCCAGAAGCTGACGCCAGTACTCACTACGcgcgccg                                                         >  2:5457939/1‑140 (MQ=255)

                                                                             cGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctg                                                                                                             <  2:2919145/87‑1 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   >  2:4192115/1‑112 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                   <  1:4192115/112‑1 (MQ=255)

                                                                              gATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccgcc                                                       <  1:8467138/140‑1 (MQ=255)

                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  1:5830924/140‑1 (MQ=255)

                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                   <  1:4300943/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCCTCCTTGCGTCGCACTTTGCTTCCCCCTCAT‑‑CCCCCCCCTTACGTTAATGGATCTGGTGACCTCTGATAGCCGCCACCATCTGAAGCCAGTACTGAATACGGGAACCGcccccctt                                                 <  2:4987705/140‑4 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:9328549/140‑1 (MQ=255)

                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                 <  1:4830427/140‑1 (MQ=255)

                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGTTATCCGCCCGCCGCGGCCGCCAGTACTCCCGACGCGCCCCGCCCCCCATcca                                              >  2:1964574/1‑138 (MQ=255)

                                                                                       ggATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGGAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCCGGACTCACGACGCGCCCCGCCCCCACTCaa                                              >  1:2587981/1‑140 (MQ=255)

                                                                                            cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                         <  1:2452780/140‑1 (MQ=255)

                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCAAC‑‑CTCCCGCCCCGTACGGTACTCGCGCTGCTGACCGCTGAGAGGCGCCCGGATCTGTCGCGAGTCCGCACTAGGCGCGCCGCCCCCGAGCAACGGAac                                        >  2:6849555/1‑140 (MQ=255)

                                                                                                   ctcggcgcCAGCCTTCCTGCGCCCACTGCCGGCCCCACA‑‑ACCCCCCCCTTACGGAAATCGACCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCCCCCCGCCCCCCAACACCGTAACACTTTg                                  <  2:7719539/136‑1 (MQ=255)

                                                                                                   ctCTGCGCCAGCCTTGCTGCGCCCAGTGNTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:5881574/139‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:3871701/140‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  2:8428422/140‑1 (MQ=255)

                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTg                                  <  1:4810379/140‑1 (MQ=255)

                                                                                                    tCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGTGCTGCTGACCGCTGAGATCCGCCAGCATCTGACGCCCGTACTCAGTAGGCGCCCCGCCCCCAATCAGCGTGAGACTTTgg                                 >  2:5236822/1‑139 (MQ=255)

                                                                                                           cAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTaa                          <  1:3927732/140‑1 (MQ=255)

                                                                                                             gCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCGCCCCGTCCGGGAAGCGAGCTGCTGACCGCTGATAGCCGCCGGCTGCTGACGCCAGTAGTCTCTACGCGCCCCGCCCCCAATCAACGTACCCCGTTGCgggggcagc                        >  2:10235818/1‑133 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGTGCTGCTGGCCGGGGATATCCGGCAGCTGCGGACGGCAGGTCGCTCTACGCGCCCCGCCCCCACTCAACGGTAAACGTTGCGGtgtgattc                       >  2:5467524/1‑133 (MQ=255)

                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGTTATCCGCCAGCATCTGACGCCAGTTCTCACGACGCGCCCCGCCCCCAATCAACGGGACACTGTGCGGTTTAGTAc                       >  1:3895575/1‑140 (MQ=255)

                                                                                                               cTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑‑CCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                     <  1:4028910/140‑1 (MQ=255)

                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                     <  1:4971268/140‑1 (MQ=255)

                                                                                                                  gctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTACTCGAGCTGCTGGCCGCTGTTATCCGCCAGCCGCGGACGCCAGGACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACGTTGCGGTGTAATACCGCa                   >  2:3927783/1‑140 (MQ=255)

                                                                                                                  gctgcGACCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCATCTGACGCCAGTACTCCCTACGCGCCCCGCCCCCAAGCCACGTTACACTGTGCGGGTTAATACCGCa                   >  2:10124220/1‑140 (MQ=255)

                                                                                                                     gcgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCGTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGTTTTATACCGCAGGc                >  1:5445198/1‑140 (MQ=255)

                                                                                                                     gcgcCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGAGATCCGCCAGCATCTGACGCCAGTACGCACTACGCGCCCCGCCCCCAATCAACGTTACACGTTGCGGGTTAATACCGCAGGc                >  1:5443838/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACGT‑‑GCCCCCCCGTTCGGTAATCGGGCTGCTGACCGCTGGTATGCGCCAGCGTCTGGCGCCAGGTCTCCCTGCGCGCCGCGCCCCCAATCAACGTAACACTTTGCGGGTTATTAGCGCTGGCagg             >  2:2080477/1‑138 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTGCGGTAATCGAGCTGCTGACCGCGGATATCCGCCAGCCTCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAAGCAACGTTACACTTTGCGGTTTAATACCGCAGGCATg             >  1:1553167/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTCATCGTGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTGACACGTTGCGGTTTAATACCGCAGGCGTg             >  1:9659871/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGGACGCACTACGCGCCCCGCCCCCCATGAGCGTAACACTTTGCGGTTTAATACCGCAGGCCTg             >  2:6544597/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCGGCTGGCCGCTGATATCCGCCAGCGTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCGACGTAACCCTTTGCGGTTTAGTACCCCAGGCATg             >  1:363892/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGTCATCGAGCTGCTGACCGCTGGTATCCGCCCGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCCATCAACGGACCACTTTGCGGGTTAGTACCGCAGGCATg             >  1:3051454/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCAC‑‑CTGCCCCCCCGTACGGTCATCGCGCTGCTGGCCGGGGATATCCGCCAGCATCTGGCGGCAGTAGTCACTACGCGCCCCGCCCCCCATCAACGGAACACTTTGCGGTTTATTACCGCAGGCATg             >  1:1553058/1‑140 (MQ=255)

                                                                                                                        cccAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAATCGAGCTGCTGTCCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCACTCAACGGAACACTGTGCGGTTTAATACCGCAGGCATg             >  1:7103883/1‑140 (MQ=255)

                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc          <  1:4392311/140‑1 (MQ=255)

                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCAc          <  1:1232114/140‑1 (MQ=255)

                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACGACGCGCCCCGCCCCCAATCCACGGCACTCTTTGCGGGTTAATTCCGCCGGCATGCACgg        >  1:1815184/1‑139 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc   >  1:10205080/1‑140 (MQ=255)

                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCAGCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAGACCGCAGGCATGCACGACAGcc   >  1:7156330/1‑140 (MQ=255)

                                                                                                                                   ccccACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCa  <  1:445244/140‑1 (MQ=255)

                                                                                                                                   ccccACAT‑‑CCCCCCCCGTACGGGACTCGAGCAGCGGACCGCTGATCTCCGCCAGCATCTGCCGCCAGTACGCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAAGACCGCAGGCATGCACGCCATCCa  <  2:5977925/140‑1 (MQ=255)

                                                                                                                                     ccccccacccccccccGACCGGTAAGCGAGCGGCTGCCCCCGCAGATCCGCCAGCATCAGACGCCAGAACTCACTACGCGCCCCGCCCCCAATCAACGTCACACTTTGCGCTTAACCACCGCAGGCAAGCACGACAGCCa  <  2:10763796/133‑1 (MQ=255)

||

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCCA  >  NZ_CP025268/3728661‑3728933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`