BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	581,197	+G	intergenic (+50/+4)	CXP41_RS24355 → / ← CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	581,196	1	.	G	97.7%	2702.5 / 45.1	792	intergenic (+49/+5)	CXP41_RS24355/CXP41_RS03085	tail fiber assembly protein/class I SAM‑dependent methyltransferase
Reads supporting (aligned to +/- strand): ref base . (12/6); new base G (444/329); total (456/336)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 810 total aligned reads displayed.

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑T‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTG  >  NZ_CP025268/581057‑581332

     ggAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑T‑ggatg                                                                                                                                     <  1:6704844/140‑5 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                <  1:6574061/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                <  2:10456819/140‑1 (MQ=255)

                           gAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaa                                                                                                                >  1:969462/1‑140 (MQ=255)

                             aCCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                              <  1:3606638/140‑1 (MQ=255)

                              ccGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAAc                                                                                                             >  1:8817065/1‑140 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACCTGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                           >  2:6937431/1‑140 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                           <  1:5051146/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                           <  1:6056014/140‑1 (MQ=255)

                                gtgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                           >  2:5096634/1‑140 (MQ=255)

                                gtggtgAGACATCAACTGCACCTGCTATCGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGAGTATGCCGCAGCCCGTCTTAAGCAAGAACAGGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                           <  1:1152225/140‑1 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGGACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                          >  1:7755816/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                          >  1:2517317/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                          >  2:6104872/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                          >  2:1554672/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                          <  2:2577857/140‑1 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                          >  2:3870649/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                          >  1:5171832/1‑140 (MQ=255)

                                 tgtTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAtt                                                                                                          >  2:4277834/1‑140 (MQ=255)

                                   tagATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCATGGAGTAATCATTGGGATTATGCCGCAGCACGTCATAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCATGAAGCGGAAAACATtac                                                                                                        >  2:9445098/3‑140 (MQ=255)

                                       tACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACa                                                                                                    <  1:5799612/140‑1 (MQ=255)

                                       tACATCAACTGAACCTGATATTGAGTGGCATACGAACCCTGTCAGTGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAATATTACTACa                                                                                                    <  2:9792794/140‑1 (MQ=255)

                                        aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTTAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                   <  2:3493204/140‑1 (MQ=255)

                                        aCATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAg                                                                                                   <  1:1744409/140‑1 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGTAG‑GATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                >  1:9290166/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACAt                                                                                                           <  1:4406874/129‑1 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                >  1:4654514/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                >  2:2905896/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                >  2:5274704/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                >  2:4954382/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                >  1:1010393/1‑140 (MQ=255)

                                           tCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAc                                                                                                >  2:7763564/1‑140 (MQ=255)

                                            cAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTAcc                                                                                               <  2:8292467/140‑1 (MQ=255)

                                                tGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGa                                                                                           >  1:6347349/1‑140 (MQ=255)

                                                tGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGa                                                                                           >  1:5560845/1‑140 (MQ=255)

                                                  cACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAAc                                                                                         >  2:4093827/1‑140 (MQ=255)

                                                    ccTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCt                                                                                       <  1:8182042/140‑1 (MQ=255)

                                                     cTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCtt                                                                                      <  2:7501512/140‑1 (MQ=255)

                                                             gAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTaa                                                                              <  1:896423/140‑1 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGTACCTTGGTTTTAAc                                                                             >  2:9671722/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                             >  1:3888087/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                             >  1:9969491/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                             >  1:5956069/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                             >  1:2878084/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                             >  1:3096937/1‑140 (MQ=255)

                                                              aGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAAc                                                                             >  2:7057244/1‑140 (MQ=255)

                                                               gTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACa                                                                            >  1:2339928/1‑140 (MQ=255)

                                                                  gCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGACAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtc                                                                         >  2:6739590/1‑140 (MQ=255)

                                                                   ccTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATtct                                                                        >  1:7609690/1‑140 (MQ=255)

                                                                       cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                    <  2:5500293/140‑1 (MQ=255)

                                                                       cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                    >  1:9866910/1‑140 (MQ=255)

                                                                       cGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                    >  2:568085/1‑140 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                   <  2:8014990/140‑1 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                   <  2:7395656/140‑1 (MQ=255)

                                                                        gAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaaa                                                                   >  2:1140100/1‑140 (MQ=255)

                                                                            ccTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGct                                                               <  1:4006577/140‑1 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                              >  2:10135354/1‑140 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                              >  1:320350/1‑140 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                              >  1:10703699/1‑140 (MQ=255)

                                                                             cTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGACTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctc                                                              >  2:10400629/1‑140 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGaaaa                                                                                                              <  2:1846747/91‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGaa                                                                    <  1:1921747/133‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                             <  2:455072/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                             <  1:8301725/140‑1 (MQ=255)

                                                                              tGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGctct                                                             >  2:5825672/1‑140 (MQ=255)

                                                                               gTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTg                                                            >  2:4848122/1‑140 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                           <  2:3217278/140‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                           <  1:10610823/140‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                           <  1:2213678/140‑1 (MQ=255)

                                                                                tCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                           <  2:8915194/140‑1 (MQ=255)

                                                                                gcAGGGAGTAATCAGTGGGATTATGCCGCAGCACGGCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                           <  1:8446103/139‑1 (MQ=255)

                                                                                 cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCtt                                                                                             >  2:9116540/1‑105 (MQ=255)

                                                                                 cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCtt                                                                                             <  1:9116540/105‑1 (MQ=255)

                                                                                 cAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTgag                                                          >  1:9514428/1‑140 (MQ=255)

                                                                                   gggAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGagag                                                        <  1:4964698/140‑1 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                     >  1:2113932/1‑140 (MQ=255)

                                                                                      aGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGtat                                                     <  1:7956387/140‑1 (MQ=255)

                                                                                        taagccttGGGATGATGCCGCTGCACGTCTTAAGCCAGAACATGCTGCGGT‑TGGATGCTCTTTTTTTCCTGAAGCGGTAAACATTACTACAGTACCTTGAACAGTGGGTTTAACATTCTCGAACTGCGCGGCGAGTagat                                                   >  2:6197401/7‑140 (MQ=255)

                                                                                        tAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAATCATTACTACAGTACCTTGAACCTTGGTTTTATCATTCTCGAAATGCTCTGAGAGTatat                                                   >  2:5950235/1‑140 (MQ=255)

                                                                                          aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                 >  1:1506981/1‑140 (MQ=255)

                                                                                          aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                 >  2:8730498/1‑140 (MQ=255)

                                                                                          aTCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATAtgt                                                 >  1:7097588/1‑140 (MQ=255)

                                                                                              ttGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTa                                             >  1:5460115/1‑140 (MQ=255)

                                                                                                gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGGGTATATGTGTTAAg                                           >  1:10803795/1‑140 (MQ=255)

                                                                                                gggATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAg                                           >  2:2180310/1‑140 (MQ=255)

                                                                                                       tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttctt                                    >  1:10210372/1‑140 (MQ=255)

                                                                                                       tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttctt                                    >  1:450849/1‑140 (MQ=255)

                                                                                                       tGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCcttctt                                    >  2:2408030/1‑140 (MQ=255)

                                                                                                                 cGTCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGGGTTAAGCCTTCTTCGGAATCttt                          >  1:7332266/1‑140 (MQ=255)

                                                                                                                   tCTTAAGCAAGATCATGCTGCGGT‑TGGATGCTATTTTTGTCCTGACGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtg                        <  2:10837733/140‑1 (MQ=255)

                                                                                                                   tCTTAAGCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTtg                        >  1:4862518/1‑140 (MQ=255)

                                                                                                                         gCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGtttt                  >  2:9484347/1‑139 (MQ=255)

                                                                                                                         gCAAGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTg                  >  2:3106348/1‑140 (MQ=255)

                                                                                                                            aGAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGaaa               >  1:6955475/1‑140 (MQ=255)

                                                                                                                             gAACATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGtt                                              <  2:2752954/108‑1 (MQ=255)

                                                                                                                              aaCATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTga                                                           >  1:4258845/1‑94 (MQ=255)

                                                                                                                              aaCATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg             >  2:649189/1‑140 (MQ=255)

                                                                                                                              aaCATGCTGCGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAg             >  1:3721957/1‑140 (MQ=255)

                                                                                                                                      gcGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCttt     <  1:6152452/140‑1 (MQ=255)

                                                                                                                                      gcGGT‑TGGATGCTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTACGGAATCTTTTGTGTTTGAAAAGATGCCttt     <  2:9217301/140‑1 (MQ=255)

AGCCTGGAAAAAATATCGGGTGTTGCTGAACCGTGTTGATACATCAACTGCACCTGATATTGAGTGGCCTACGAACCCTGTCAGGGAGTAATCATTGGGATTATGCCGCAGCACGTCTTAAGCAAGAACATGCTGCGGT‑T‑GATGTTATTTTTTTCCTGAAGCGGAAAACATTACTACAGTACCTTGAACCTTGGTTTTAACATTCTCGAAATGCTCTGAGAGTATATGTGTTAAGCCTTCTTCGGAATCTTTTGTGTTTGAAAAGATGCCTTTCTG  >  NZ_CP025268/581057‑581332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`