BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,224,095	+GA	coding (248/249 nt)	CXP41_RS06440 →	protein YmgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,224,094	1	.	A	99.4%	8308.7 / 26.1	2177	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (8/6); new base A (866/1296); total (874/1303)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	1,224,094	2	.	G	99.4%	7611.4 / 27.0	2177	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (8/6); new base G (865/1297); total (874/1303)
Fisher's exact test for biased strand distribution p-value = 2.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2317 total aligned reads displayed.

TCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑A‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGAC  >  NZ_CP025268/1223955‑1224233

||

    gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaa                                                                                                                                           >  2:5608751/1‑137 (MQ=255)

    gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑agaa                                                                                                                                           >  2:3662829/1‑137 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTa                                                                                                                                   <  1:9031782/140‑1 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAg                                                                                                                                 >  2:9508856/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAg                                                                                                                                 >  1:4949549/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑AGACCTTGAGTAAg                                                                                                                                 >  2:1989585/1‑140 (MQ=255)

               gCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAgg                                                                                                                                >  1:8850360/1‑140 (MQ=255)

                  tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGttt                                                                                                                             <  2:7672011/140‑1 (MQ=255)

                       aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGaa                                                                                                                        <  1:2827495/140‑1 (MQ=255)

                       aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGaa                                                                                                                        <  2:10451032/140‑1 (MQ=255)

                       aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGaa                                                                                                                        <  2:2730096/140‑1 (MQ=255)

                       aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGaa                                                                                                                        <  2:9064070/140‑1 (MQ=255)

                         gATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATa                                                                                                                      <  2:7460232/140‑1 (MQ=255)

                            gAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACAGTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAAc                                                                                                                   >  1:6164129/1‑140 (MQ=255)

                             aaaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACa                                                                                                                  >  1:1296399/1‑140 (MQ=255)

                             aaaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACa                                                                                                                  <  1:763032/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  2:2950926/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  2:5054023/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  1:727113/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  1:3799024/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  1:1067359/140‑1 (MQ=255)

                                ccAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCa                                                                                                               <  1:237571/140‑1 (MQ=255)

                                 cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                              <  1:3673832/140‑1 (MQ=255)

                                    tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTNCACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                           >  1:5908941/1‑140 (MQ=255)

                                    tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                           <  1:9579713/140‑1 (MQ=255)

                                    tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                           >  2:8474851/1‑140 (MQ=255)

                                            tttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCa                                                                                                   <  2:6788121/140‑1 (MQ=255)

                                            tttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCa                                                                                                   <  1:10202658/140‑1 (MQ=255)

                                            tttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCa                                                                                                   >  1:1106309/1‑140 (MQ=255)

                                              tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                                 <  2:6540018/140‑1 (MQ=255)

                                              tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                                 <  2:1263711/140‑1 (MQ=255)

                                                   tctcTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGAt                                                                                            <  1:1819567/140‑1 (MQ=255)

                                                   tccctATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGAt                                                                                            <  1:5423428/137‑1 (MQ=255)

                                                       tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                                        >  2:10000548/1‑140 (MQ=255)

                                                       tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                                        >  1:3986497/1‑140 (MQ=255)

                                                             cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                                  <  2:539023/140‑1 (MQ=255)

                                                             cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                                  <  2:4910702/140‑1 (MQ=255)

                                                             cAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAg                                                                                  <  1:7441270/140‑1 (MQ=255)

                                                                    tGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCa                                                                           <  2:2157592/140‑1 (MQ=255)

                                                                       aGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGc                                                                        >  1:918305/1‑140 (MQ=255)

                                                                       aGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGc                                                                        >  1:1982692/1‑140 (MQ=255)

                                                                           cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                                    >  1:4685155/1‑140 (MQ=255)

                                                                           cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                                    >  2:5790471/1‑140 (MQ=255)

                                                                             ttGTAATGCATATGACTACACTAACGTATATCATTATTACTGACAGGGAATCCGTGCTCTCTTAGA‑A‑GTTGAGTAAGGTTTTAGAATAAACATCTGGTGTATCACCATGCTGATTGTAAGTGAGCATTTCAGGCGTTATg                                                                  <  1:6860884/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGACTCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  2:4088996/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  2:3132847/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  1:4798947/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  1:10000548/140‑1 (MQ=255)

                                                                              tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGttt                                                                                                                             >  2:3855057/1‑80 (MQ=255)

                                                                              tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                                 >  2:17455/1‑140 (MQ=255)

                                                                              tGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAAACCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGc                                                                 >  2:10216263/1‑140 (MQ=255)

                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                                <  1:8429556/140‑1 (MQ=255)

                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                                <  1:2571649/140‑1 (MQ=255)

                                                                                aaaTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCtt                                                               >  2:8197444/1‑140 (MQ=255)

                                                                                   tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                            >  1:3466045/1‑140 (MQ=255)

                                                                                   tGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCt                                                            >  1:3220143/1‑140 (MQ=255)

                                                                                    gCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtt                                                           <  2:10922483/140‑1 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                          >  1:4542883/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                          <  1:6719111/140‑1 (MQ=255)

                                                                                        aaGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                           <  1:6557655/88‑1 (MQ=255)

                                                                                         aGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTtattat                                                      <  1:8043006/140‑1 (MQ=255)

                                                                                         aGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTtattat                                                      <  2:2367239/140‑1 (MQ=255)

                                                                                          gACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATg                                                     >  1:6331118/1‑140 (MQ=255)

                                                                                          gACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTAGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATg                                                     >  1:1710854/1‑140 (MQ=255)

                                                                                            cTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTc                                                   <  2:7122445/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                                 <  2:6307547/93‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                                  >  1:8284672/1‑140 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                                  <  2:5965604/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                                  <  2:9255615/140‑1 (MQ=255)

                                                                                              acacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                                 <  2:865515/140‑1 (MQ=255)

                                                                                              acacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                                 <  2:5217142/140‑1 (MQ=255)

                                                                                              acacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                                 <  2:4301689/140‑1 (MQ=255)

                                                                                              acacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                                 <  2:10697569/140‑1 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGACTCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                                >  1:4306414/1‑140 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                                >  1:5232116/1‑124 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                           >  1:10352888/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCCTATTATGTCCGCAatat                                           >  2:3331305/1‑140 (MQ=255)

                                                                                                     cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                          >  2:7309533/1‑140 (MQ=255)

                                                                                                     cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                          >  1:215511/1‑140 (MQ=255)

                                                                                                     cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                          <  2:2537924/140‑1 (MQ=255)

                                                                                                     cGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATc                                          >  1:4147944/1‑140 (MQ=255)

                                                                                                      ggATATCATTATTAATGACCGGGCATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTGTAGTATAAACATCCGCTGTATCCCCATGCTGATGCCATGTGAGCAGTTCGGGCGGTATGCTGTCGTGTTATGGGCGCAatagct                                         >  2:6102928/1‑137 (MQ=255)

                                                                                                      ggATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCa                                         <  2:465212/140‑1 (MQ=255)

                                                                                                       gATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAg                                        >  1:5606469/1‑140 (MQ=255)

                                                                                                       gATATCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAg                                        <  2:7005328/140‑1 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                                  >  2:8851278/1‑128 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                                      >  2:4470025/1‑140 (MQ=255)

                                                                                                            cATTATTAATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTc                                   >  1:1532067/1‑140 (MQ=255)

                                                                                                              ttattaATGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCaa                                 >  1:10514015/1‑140 (MQ=255)

                                                                                                                    aTGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATgg                           <  1:9721337/140‑1 (MQ=255)

                                                                                                                    aTGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATgg                           <  1:7060161/140‑1 (MQ=255)

                                                                                                                     tGACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGga                          <  1:2990946/140‑1 (MQ=255)

                                                                                                                      gACAGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag                         <  1:5807379/140‑1 (MQ=255)

                                                                                                                         aGGGAATCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGt                      >  1:8612323/1‑140 (MQ=255)

                                                                                                                             aaTCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATACACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtctc                  <  2:1503507/140‑1 (MQ=255)

                                                                                                                             aaTCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtctc                  <  1:9357374/140‑1 (MQ=255)

                                                                                                                             aaTCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtctc                  <  1:5060236/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg                 <  1:7813280/140‑1 (MQ=255)

                                                                                                                                ccGTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGct               <  2:7887465/140‑1 (MQ=255)

                                                                                                                                  gTGCTCTCTTAGA‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGctct             <  2:974660/140‑1 (MQ=255)

||

TCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCTT‑‑A‑A‑CTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGAC  >  NZ_CP025268/1223955‑1224233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`