BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,374,862	(T)_8→9	intergenic (+40/‑172)	pspE → / → ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,374,854	1	.	T	94.4%	1703.4 / 90.4	544	intergenic (+32/‑180)	pspE/ycjM	thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand): ref base . (13/17); new base T (285/221); total (298/246)
Fisher's exact test for biased strand distribution p-value = 1.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.42e-02

Only 100 of 594 total aligned reads displayed.

GGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCG  >  NZ_CP025268/1374715‑1374989

 gACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAGGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑t                                                                                                                                          >  2:1334465/1‑140 (MQ=255)

        gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttt‑t‑ttt                                                                                                                                 <  1:9222021/140‑1 (MQ=255)

        gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttt‑t‑ttt                                                                                                                                 >  2:8065840/1‑140 (MQ=255)

        gTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttt‑t‑ttt                                                                                                                                 >  1:3035981/1‑140 (MQ=255)

             ggCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttt‑t‑ttttctgt                                                                                                                            >  2:9450750/1‑135 (MQ=255)

             ggCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttt‑t‑ttttctgt                                                                                                                            <  1:3691791/140‑6 (MQ=255)

              gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTc                                                                                                                            <  1:6241360/140‑1 (MQ=255)

              gcaagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTc                                                                                                                            <  1:2855033/140‑1 (MQ=255)

               caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTca                                                                                                                           <  2:964560/140‑1 (MQ=255)

               caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTca                                                                                                                           <  1:4505413/140‑1 (MQ=255)

               caagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTca                                                                                                                           <  2:4231903/140‑1 (MQ=255)

               caagcaaAAGAGATCCTGAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACCTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTca                                                                                                                           <  1:5294994/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTcac                                                                                                                          <  2:9842784/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTcac                                                                                                                          <  1:6923237/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTcac                                                                                                                          <  1:2736995/140‑1 (MQ=255)

                aagcaaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTcac                                                                                                                          <  2:9106225/140‑1 (MQ=255)

                   caaAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACAcc                                                                                                                       >  2:10751884/1‑140 (MQ=255)

                        gagaTCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTAtt                                                                                                                  <  1:10309704/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACa                                                                                                              <  1:6512141/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACa                                                                                                              <  2:7003671/140‑1 (MQ=255)

                            tCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACa                                                                                                              <  2:10365367/140‑1 (MQ=255)

                             ccTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTATGTCACACCATATTTACAt                                                                                                             >  1:1101196/1‑140 (MQ=255)

                                tAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATccc                                                                                                          <  1:6686896/140‑1 (MQ=255)

                                    gagaTGGGATATACCCACTTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTAAAAGGTTAAAAGAAGTCACCATGAGCTGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATa                                                                                                      <  2:8684685/140‑1 (MQ=255)

                                    gagaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATa                                                                                                      <  2:4385502/140‑1 (MQ=255)

                                      gaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAACGGTTAAAAGAATTCACCATGAGCGTCGCTGATGCGCCG‑‑TTTTAT‑TTTCTGTCACACCTTATTTACATCCCCATAGa                                                                                                    <  2:661472/140‑1 (MQ=255)

                                      gaTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGTGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGa                                                                                                    >  2:3896407/1‑140 (MQ=255)

                                       aTGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGat                                                                                                   >  1:10464445/1‑140 (MQ=255)

                                          ggATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGattat                                                                                                <  1:4104027/140‑1 (MQ=255)

                                            atatACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTAttt                                                                                              >  1:5749604/1‑140 (MQ=255)

                                            atatACCCAAGTTGAGAACGCCTGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTAAAAAGAATACACCATGAGCGGCGCTTCTGCGCCG‑‑TTTT‑TGTTTCTGTCACACGTTATTTACATCCCCATAGATTAatt                                                                                              >  2:1465618/1‑140 (MQ=255)

                                                aCCCACGTTGAGAACGCCGTTGGCCTGAAAGACATCGCAATGCCGAATGTCAAAGTTTAAAAGAATTCACCATGAGCGGAGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                          <  2:1819173/140‑1 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTGAAACCTTTTTTACATCCCCATAGATTATTTGCGt                                                                                          >  2:2157114/1‑140 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGt                                                                                          <  1:9545263/140‑1 (MQ=255)

                                                aCCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTATGTCACACCGTATTTACATCCCCATAGATTATTTTCGt                                                                                          >  1:3305089/1‑140 (MQ=255)

                                                  ccACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCa                                                                                        >  2:3176497/1‑140 (MQ=255)

                                                   cACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAg                                                                                       <  1:9743115/140‑1 (MQ=255)

                                                    aCGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCGTATTTACATCCCCATAGATTATTTGCGTCAGc                                                                                      >  1:5422767/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCCTGAGCGGCGCTTAGGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:2309515/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  1:3494387/1‑140 (MQ=255)

                                                     cGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCt                                                                                     >  2:5596364/1‑140 (MQ=255)

                                                      gTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTTGT‑TTTCTGTCACACCTTAGTTACATCCCCATAGATTATTTGCGTCAGCTc                                                                                    >  2:9249374/1‑140 (MQ=255)

                                                       ttGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGTTTATTTGCGTCAGCTca                                                                                   <  1:4980220/140‑1 (MQ=255)

                                                        tGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTcac                                                                                  <  2:8519938/140‑1 (MQ=255)

                                                         gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttt‑t‑tttt                                                                                                                                >  2:4764547/1‑91 (MQ=255)

                                                         gagaACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑tttt‑t‑tttt                                                                                                                                <  1:4764547/92‑2 (MQ=255)

                                                                ccGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGTTTTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACg                                                                           >  2:4879975/1‑140 (MQ=255)

                                                                acGGTGGCCTTAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAATAATTCACCATGAGCGGAGCATATGCGCCGT‑TTTT‑T‑TTTATTTCAAAACTTATTTACATCCCCATAGATTATTTTCTTCAGCTAAAAAATAAGc                                                                          >  2:3050455/2‑140 (MQ=255)

                                                                    tGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCtttt                                                                      >  2:3398177/1‑140 (MQ=255)

                                                                       ccTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTcc                                                                   <  1:6748174/140‑1 (MQ=255)

                                                                         tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCt                                                                 <  1:2037765/140‑1 (MQ=255)

                                                                         tGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTATGTCACACCTTATTTACATACCCAAATTTTATTTTCGTCAGCTCACAAATAAGCTTTTTaccg                                                                 >  1:598009/1‑139 (MQ=255)

                                                                           aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGa                                                                                                    <  2:2615340/103‑1 (MQ=255)

                                                                           aaaGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGCTTATTTGCGTCAGCTCACAAATACGCTTTTTcgcggg                                                               >  1:8781586/1‑135 (MQ=255)

                                                                                cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaa                                                          >  1:1646235/1‑140 (MQ=255)

                                                                                cATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTACCTGGTaaaa                                                          >  2:7891515/1‑140 (MQ=255)

                                                                                 aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTGTGTCACACATTATATACAACCAAATAGATTGTTTGCGTCAGCTCTCAAATAGGCTTTTTCCCTGGTaaaaa                                                         >  2:8820854/1‑140 (MQ=255)

                                                                                 aTCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTATGTCACACCGTATTTAAAAACCCATAGATTATTTTCGGCAGCTCACAAATAAGCTATTTTCCAGGGaaaaa                                                         >  1:7547266/1‑140 (MQ=255)

                                                                                  tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTaaaaaa                                                        >  2:1194783/1‑140 (MQ=255)

                                                                                  tCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCGTATGCGCCGT‑TTTT‑T‑TTTGTGTGAACCCGTATTTACAACCCCAAAGATTATTTTCGTCAGCGCACAAATACGCATTTTAACAGGTaaaaaa                                                        >  1:5140755/1‑140 (MQ=255)

                                                                                   cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                       >  2:10696754/1‑140 (MQ=255)

                                                                                   cGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAAt                                                       >  2:3924061/1‑140 (MQ=255)

                                                                                         gCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTc                                                 >  2:7116680/1‑140 (MQ=255)

                                                                                             aaGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGc                                             >  1:9151388/1‑140 (MQ=255)

                                                                                              aGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCg                                            <  1:3924061/140‑1 (MQ=255)

                                                                                                gTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTg                                          <  1:7803099/140‑1 (MQ=255)

                                                                                                  cAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGA‑ATTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGAc                                        <  1:10220087/140‑1 (MQ=255)

                                                                                                    aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCAGCGTGACTa                                      >  1:1745371/1‑140 (MQ=255)

                                                                                                    aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTATGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTACTGCGTGTCTa                                      >  1:9925827/1‑140 (MQ=255)

                                                                                                    aaGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTATGTAACACCCTATTTACATACCCAAATATTATTTGAGGAAGCTCACAAAAAAGCTTTTTACCTGGTAAAAAAATATTTAATGCGTGACTa                                      >  1:2473229/1‑140 (MQ=255)

                                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTTTGTCAAACCGTATTTACAAACCCAAAGATTATTTGCGTCATCTCACAAATAAGCTTTTTAACTGGTAAAAAATGTTTTACTGCGTGACTaaaaaac                                >  1:4321727/1‑137 (MQ=255)

                                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                                >  2:685026/1‑140 (MQ=255)

                                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                                >  2:3576112/1‑140 (MQ=255)

                                                                                                          aaaaGAATTCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTATTTTACACCTTATTTAAATACCCATAGTTTATTTTCGTCAGCTCACAAATAAGCATTTTACCAGGTAAAAAAAGATTTAAATCGTGACTAAAAacc                                >  1:1074005/1‑140 (MQ=255)

                                                                                                          aaaaGAATTCACAATGAGCGGCGCGTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCGTATTTACATCCCCAAAGATTATTTGCGTCAGCTCACAAATACGCTTTTTAACTGGTAAAAAATGATTTCCTGCGTGACTAAAAccc                                >  2:10534786/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTTTGAAACCTAATTTAAATACCCATAGATTAATTGGGTCAGCTCACAAATAAGCTTTTTACCTTGTAAAAAATGATTTAGAGCGTGACAAAAAACCAtgtggt                         >  1:2284612/1‑138 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                         >  2:4135211/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                         >  1:7243305/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                         >  2:1733667/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCt                         >  2:8276546/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTACCTGGTAGAAAATGTTTTCCTGCGTGACTAAGACCCTTGTGCt                         >  1:8869202/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTACCTGGTAAAAAATGATTTACTGCGTGACTAAAACCCTAGTGCt                         >  2:7798413/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTAAATTACCTCCCCATAGAATAATTGCGTCAGCTCACAAATACGCATTTTACCTGGGAAAAAATTATTTACAGCCTGACTAAAACCCATGTGCt                         >  2:6257412/1‑140 (MQ=255)

                                                                                                                 ttCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCGTATTTACATACCCAAAGATTATTTGCGTCAGCACACAAAAAAGCTTTTTCCCTGGTAAAAAAATATTTCCTGCGTGACTAAAACCCTATTGCt                         >  1:4241514/1‑140 (MQ=255)

                                                                                                                  tCACCATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTc                        >  2:6062458/1‑140 (MQ=255)

                                                                                                                     ccATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAAt                     <  1:7074852/140‑1 (MQ=255)

                                                                                                                      cATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTGTGTCACACCTTATTTAAATACCCATAGATTATTTTCGTCAGCACACAAAAAAGCATTTTCCCTGGTAAAAAATTATTTACAGCGTGTCTAAAAACCATTTGCTCAAtt                    >  1:1425279/1‑140 (MQ=255)

                                                                                                                      cATGAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACGAAATCCCTTGTGCTCAAtt                    >  2:6959010/1‑140 (MQ=255)

                                                                                                                         gAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCa                                                                                                        >  2:8385558/1‑53 (MQ=255)

                                                                                                                         gAGCGGCGCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGAc                 <  1:10068952/140‑1 (MQ=255)

                                                                                                                           gcggcgCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTTCGTCAGCACACAAATACGCTTTTTCACTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAg               >  2:7398381/1‑140 (MQ=255)

                                                                                                                           gcggcgCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAAACCTTGTGCTCAAATGACat               >  2:5803001/1‑139 (MQ=255)

                                                                                                                            cggcgCTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGt              <  2:10237637/140‑1 (MQ=255)

                                                                                                                              gcgcTTATGCGCCGT‑TTTT‑T‑TTTCTGTCCCACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCAGGTAAAAAATGATTTCCTGCGTGACAAAAACCCGTGTGCTCAATTGGCAGttt            >  2:5323672/1‑140 (MQ=255)

                                                                                                                              gcgcTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGttt            <  1:3196190/140‑1 (MQ=255)

                                                                                                                                gcTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACAACTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATAAGCTTTTTCCCTGGTAAAAAATTATTTACTGCGTGACTAAAACCCATGTGCTCAATTGTCAGTTTAt          >  1:10821948/1‑140 (MQ=255)

                                                                                                                                 cTTATGCGCCGT‑TTTT‑T‑TTTCTGTCTCACATTATTTACTTGCACATAGTTTATTTGCTTAAGCTCTGTAATAAGCTTTTTAACTGGTAAAATTTGATTTGATGCGTGACTAAAACCCTTGTGCTCTTTTTTCAGtttttt         <  1:8585041/140‑1 (MQ=255)

                                                                                                                                 cTTATGCGCCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTAtt         <  1:8065840/140‑1 (MQ=255)

                                                                                                                                      gcgcCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg    <  1:8298097/140‑1 (MQ=255)

                                                                                                                                      gcgcCGT‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTg    <  1:1259574/140‑1 (MQ=255)

GGACGCCAGTCAGGGCAAGCAAAAGAGATCCTTAGCGAGATGGGATATACCCACGTTGAGAACGCCGGTGGCCTGAAAGACATCGCAATGCCGAAGGTCAAAGGTTAAAAGAATTCACCATGAGCGGCGCTTATGCGCCG‑‑TTTT‑T‑TTTCTGTCACACCTTATTTACATCCCCATAGATTATTTGCGTCAGCTCACAAATACGCTTTTTCCCTGGTAAAAAATGATTTCCTGCGTGACTAAAACCCTTGTGCTCAATTGACAGTTTATTTTCTGCG  >  NZ_CP025268/1374715‑1374989

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`