BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,660,178	+C	coding (314/351 nt)	ynfA ←	YnfA family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,660,174	1	.	C	98.0%	1756.5 / 22.7	512	coding (318/351 nt)	ynfA	YnfA family protein
Reads supporting (aligned to +/- strand): ref base . (9/1); new base C (224/278); total (233/279)
Fisher's exact test for biased strand distribution p-value = 6.75e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 560 total aligned reads displayed.

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

cGAAAAATGCCGGAGTGCTCACAATAAAAGGCGTATATTTGCGCTGTGAAGGGTTGACTCATGTTGAAAGAGAATACCTTTTGTCAGCTGAGAGTTCCTCTTATCTTCTGGATTAAGTGGGTTTGTGTTCGTACGTggg‑c                                                                                                                                        >  2:4176750/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                        >  2:3461955/1‑140 (MQ=255)

cGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTgcg‑c                                                                                                                                        >  1:5522585/1‑140 (MQ=255)

 gAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑c                                                                                                                                      >  1:10202476/1‑140 (MQ=255)

   aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccc                                                                                                                                    <  1:9157340/140‑1 (MQ=255)

   aaaaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑ccc                                                                                                                                    <  1:8532754/140‑1 (MQ=255)

     aaaGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCG‑c‑cccca                                                                                                                                  <  1:9828970/140‑3 (MQ=255)

            gAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGca                                                                                                                            >  1:10712339/1‑140 (MQ=255)

            gAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGca                                                                                                                            >  2:6632117/1‑140 (MQ=255)

             aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGATATAGAAGACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaa                                                                                                                           <  2:2502378/140‑1 (MQ=255)

             aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaa                                                                                                                           <  2:5060668/140‑1 (MQ=255)

             aGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaa                                                                                                                           <  2:4451607/140‑1 (MQ=255)

               tGATCACATAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaaca                                                                                                                         <  2:7450588/140‑1 (MQ=255)

               tGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGcaaca                                                                                                                         <  1:9515166/140‑1 (MQ=255)

                       aaaaaaGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCaa                                                                                                                 >  2:2940026/1‑140 (MQ=255)

                               cGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGc                                                                                                         <  1:8332746/140‑1 (MQ=255)

                                gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                        >  1:6066227/1‑140 (MQ=255)

                                gTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCa                                                                                                        >  2:8853378/1‑140 (MQ=255)

                                       tGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCa                                                                                                 <  1:4464491/140‑1 (MQ=255)

                                         cgcTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAt                                                                                               >  1:4036063/1‑140 (MQ=255)

                                              tgAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACg                                                                                          >  2:5758888/1‑140 (MQ=255)

                                                aaTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCa                                                                                        >  1:6964795/1‑140 (MQ=255)

                                                 aTGGTTGACAAAAGATGAAATAGCATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       <  1:7732995/140‑1 (MQ=255)

                                                 aTGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCAc                                                                                       <  2:7144241/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACACTGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  2:8530778/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:4393838/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:2066241/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:7997535/140‑1 (MQ=255)

                                                      tGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTc                                                                                  <  1:156701/140‑1 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                <  2:9699530/140‑1 (MQ=255)

                                                        aCAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCa                                                                                >  2:2091003/1‑140 (MQ=255)

                                                            aaGATGAAATAGAATACCTGTTGTCAGCTGACACTTCCTCTTAGCATATTGATAAAATGGATTTCTGTTCCTACGTGCGCC‑CCCAGCCCGCCACACAGATCAACAGACCGAAAAGAGCCATCAACGCTCGCGGCCAGTCa                                                                            >  2:9174442/1‑140 (MQ=255)

                                                              gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                          <  2:5644335/140‑1 (MQ=255)

                                                              gATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATa                                                                          <  1:1174573/140‑1 (MQ=255)

                                                              gATGAAATAGAATACCATTTGTCAGCTGACACTTGCTCTTATCTTATTGATAAAATGGATTGATGTTCCTCCGTGCGCC‑CCCAGCCCTCAACAATGATCAACATGCCGCAAAGCGAAATCAACGCACCCGTCCAGTCATa                                                                          <  2:6165148/140‑1 (MQ=255)

                                                               aTGAAATAGAATACCTTTTGTCAGCTGACACTTCGTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACACTGATCAACATGCCGCAAAGCGCAATCTACGCACCCGTCCAGTCATat                                                                         >  2:10530569/1‑139 (MQ=255)

                                                               aTGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaa                                                                         >  2:5941317/1‑140 (MQ=255)

                                                                tGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATaaa                                                                        <  1:5259538/140‑1 (MQ=255)

                                                                   aaTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAagag                                                                     >  2:1177917/1‑140 (MQ=255)

                                                                    aTAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGt                                                                    <  1:1446689/140‑1 (MQ=255)

                                                                        aaTACCTTTTGTCAGCTGACACTTCCTCTTCTCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCTCGCAACAATGATCATCATGCCGCAAAGCGCAATCAACGCCCCCGTCCAGTCATAATGAGTCAGt                                                                >  2:8717923/1‑140 (MQ=255)

                                                                        aaTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGt                                                                >  2:9223629/1‑140 (MQ=255)

                                                                         aTACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGtt                                                               >  1:3421310/1‑140 (MQ=255)

                                                                           aCCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTc                                                             <  2:10192671/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  1:10493438/140‑1 (MQ=255)

                                                                              ttttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACg                                                          <  1:6781800/140‑1 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  1:10031083/1‑140 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  1:3168837/1‑140 (MQ=255)

                                                                               tttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGc                                                         >  2:820553/1‑140 (MQ=255)

                                                                                ttGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGcc                                                        <  2:4358109/140‑1 (MQ=255)

                                                                                     aGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCca                                                   <  2:5547152/140‑1 (MQ=255)

                                                                                       cTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCcaca                                                 >  1:2585519/1‑140 (MQ=255)

                                                                                       cTGACACTTACTATTATCTTTTTTATAAAATGGATTTATGTTCCTACGTTCG‑CACCCAGACCGCAACAATGATCAACATGCCGCAAAGCGAAATCAACTCACCAGTCAATTCATAAAGAGTCAGTTTCAAGCCATCcaaa                                                 <  2:1919971/140‑3 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              <  2:942298/140‑1 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              >  1:10636394/1‑140 (MQ=255)

                                                                                          acacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcg                                              >  1:6610029/1‑140 (MQ=255)

                                                                                          acacGTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATCAAGAGTCAGTTTCACGCCATCCACAAcg                                              <  1:3749887/140‑1 (MQ=255)

                                                                                           cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                             <  1:5781523/140‑1 (MQ=255)

                                                                                           cacTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgc                                             <  2:3777075/140‑1 (MQ=255)

                                                                                            acgtCCTCTTATCTTATTGATAAATTGGATTTATGTTCCTACGAGCGCC‑CCCAGCCCGCAACAATGATCACCATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  2:10867888/137‑1 (MQ=255)

                                                                                            acTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAAcgcg                                            <  2:6345019/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACCTGCCGCAAAGCGCAATCAACGCACCCGTCCCGTCAGAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:3037809/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        >  1:8258880/1‑140 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:9052558/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  1:9993694/140‑1 (MQ=255)

                                                                                                ccTCTTATCTTATTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGc                                        <  2:5148702/140‑1 (MQ=255)

                                                                                                ccTCTGATCTTATTGATAAAATGGATTTATGGTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCCAAGCGCACTCAACGCACCCGTCCAGTCATCAAGAGTCAGTTTCACGCCTTCCACACCGCGCAGc                                        <  2:1635173/140‑1 (MQ=255)

                                                                                                  tcttatcttaTTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCca                                      >  1:7067596/1‑140 (MQ=255)

                                                                                                    ttatcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCcaca                                    >  1:8756844/1‑140 (MQ=255)

                                                                                                     tatcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACAt                                   >  2:2327706/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCACTCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  2:2766669/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  1:6190781/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATc                                  >  2:4084703/1‑140 (MQ=255)

                                                                                                      atcttatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGGCAGTTTCACGCCATCCACCACGCGCCGCCACATc                                  >  2:6985467/1‑140 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCCCGCCATCCACAACGCGCTGCCACATCAACg                              <  2:7969784/140‑1 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  2:10449145/140‑1 (MQ=255)

                                                                                                          tatTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACg                              <  1:2905328/140‑1 (MQ=255)

                                                                                                          tatTGATAAAATGGATTGAGGTTCCTACGTGCGCC‑CCCCGCCCGCAACAAGGACCAACCTTCCGCAACGCGCCATCAACGCACCCGTCCAGTCAGAAAGAGGCAGTTTCACGCCAGCCACCACGCGCAGCCACATCaagg                              >  2:4881783/1‑138 (MQ=255)

                                                                                                           atTGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCCTCCACAACGCGCAGCCACATCAACGc                             >  1:7520189/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                            >  1:3574629/1‑140 (MQ=255)

                                                                                                            ttGATAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGcc                            >  1:10400522/1‑140 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  1:2381410/140‑1 (MQ=255)

                                                                                                               aTAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGAAACATTGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTg                         <  2:1347079/140‑1 (MQ=255)

                                                                                                                tAAAATGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGc                        <  1:4212327/140‑1 (MQ=255)

                                                                                                                 aaaaTGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCa                       <  2:4651106/140‑1 (MQ=255)

                                                                                                                  aaaTGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAg                      <  1:5171219/140‑1 (MQ=255)

                                                                                                                  aaaTGGATTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAg                      <  2:6899502/140‑1 (MQ=255)

                                                                                                                       gATTTATGTTCCTACGTGCGCC‑CCCAGCCCGAAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATa                 <  2:3214932/140‑1 (MQ=255)

                                                                                                                        aTTTATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaa                >  1:4900934/1‑140 (MQ=255)

                                                                                                                         tttATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAAACAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATaaa               <  2:7774558/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  1:9373997/140‑1 (MQ=255)

                                                                                                                           tATGTTCCTACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACg             <  2:3649398/140‑1 (MQ=255)

                                                                                                                                 cctACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       <  1:2953121/138‑1 (MQ=255)

                                                                                                                                 cctACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGccacca       >  1:8892295/3‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGTGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      <  1:7802418/139‑1 (MQ=255)

                                                                                                                                  ctACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      >  1:564244/2‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      >  1:4569419/2‑140 (MQ=255)

                                                                                                                                  ctACGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCAt      >  1:7217131/2‑140 (MQ=255)

                                                                                                                                     cGTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAg   <  1:6324241/140‑1 (MQ=255)

                                                                                                                                      gTGCGCC‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGc  <  1:3815438/140‑1 (MQ=255)

CGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTCTTATCTTATTGATAAAATGGATTTATGTTCTTACGTGCG‑C‑CCCAGCCCGCAACAATGATCAACATGCCGCAAAGCGCAATCAACGCACCCGTCCAGTCATAAAGAGTCAGTTTCACGCCATCCACAACGCGCAGCCACATCAACGCCGTGCAGACATAAACGCCACCATAAGC  >  NZ_CP025268/1660036‑1660308

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`