BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,690,360	(C)_5→6	pseudogene (1047/1403 nt)	fumC ←	class II fumarate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,690,355	1	.	C	98.4%	2362.7 / 24.5	689	pseudogene (1052/1403 nt)	fumC	class II fumarate hydratase
Reads supporting (aligned to +/- strand): ref base . (5/6); new base C (338/340); total (343/346)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 743 total aligned reads displayed.

TCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑CC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCA  >  NZ_CP025268/1690217‑1690489

tCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAgc‑g‑c                                                                                                                                          <  1:6502757/140‑1 (MQ=255)

tCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAgc‑g‑c                                                                                                                                          <  2:7927871/140‑1 (MQ=255)

   cTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTGTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTTGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑cc‑cc                                                                                                                                      <  2:3570167/140‑1 (MQ=255)

     cACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGTAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑cc‑cc‑cc                                                                                                                                   <  2:8489740/140‑2 (MQ=255)

     cACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑cc‑cc‑cc                                                                                                                                   <  2:4514486/140‑2 (MQ=255)

      aCGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑cc‑cc‑cca                                                                                                                                  <  1:115732/140‑3 (MQ=255)

        gATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑cc‑cc‑ccatg                                                                                                                                <  1:9590421/140‑5 (MQ=255)

             ggTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGAt                                                                                                                           >  2:1712789/1‑140 (MQ=255)

                  aaTACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCa                                                                                                                      >  2:10164977/1‑140 (MQ=255)

                    tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCAcg                                                                                                                    >  1:1801849/1‑140 (MQ=255)

                    tACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCAcg                                                                                                                    >  1:855585/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAGATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGtt                                                                                                               >  1:6899359/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGtt                                                                                                               >  1:3194694/1‑140 (MQ=255)

                         aCTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGtt                                                                                                               >  1:2307408/1‑140 (MQ=255)

                            gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTccc                                                                                                            >  2:3331318/1‑140 (MQ=255)

                            gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTccc                                                                                                            <  1:358257/140‑1 (MQ=255)

                            gcgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTccc                                                                                                            >  2:10231240/1‑140 (MQ=255)

                             cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGACCTTGTGGATCACGATAGGACGGAAGACGTTCAGTTCAAAGTGACCGGAAGC‑G‑CCGCC‑C‑ATGTTGATCGCCTCGTCGTTcccc                                                                                                           >  2:6118570/1‑140 (MQ=255)

                             cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTcccc                                                                                                           >  1:1112177/1‑140 (MQ=255)

                             cgcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTcccc                                                                                                           <  1:10806917/140‑1 (MQ=255)

                              gcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCa                                                                                                          >  1:1064552/1‑140 (MQ=255)

                              gcAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCa                                                                                                          >  1:5473600/1‑140 (MQ=255)

                               cAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCAt                                                                                                         <  1:4585975/140‑1 (MQ=255)

                               cAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCAt                                                                                                         <  1:4731665/140‑1 (MQ=255)

                                 gtgtTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCa                                                                                                       >  1:3057029/1‑140 (MQ=255)

                                  tgtttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCAc                                                                                                      <  1:10010031/140‑1 (MQ=255)

                                    tttgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCt                                                                                                    >  1:2546305/1‑140 (MQ=255)

                                      tgttAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGa                                                                                                  <  1:7251744/140‑1 (MQ=255)

                                         tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGCAC‑CC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                               <  2:2279446/140‑1 (MQ=255)

                                         tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                               <  2:8121752/140‑1 (MQ=255)

                                         tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                               <  1:8734007/140‑1 (MQ=255)

                                         tAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcag                                                                                               <  2:1368055/140‑1 (MQ=255)

                                            aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                            >  2:4068546/1‑140 (MQ=255)

                                            aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                            >  1:8329818/1‑140 (MQ=255)

                                            aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                            >  2:20744/1‑140 (MQ=255)

                                            aaCTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGAcagcag                                                                                            >  2:5148905/1‑140 (MQ=255)

                                               tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                         <  1:6752044/140‑1 (MQ=255)

                                               tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                         <  1:9340179/140‑1 (MQ=255)

                                               tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                         <  1:8153075/140‑1 (MQ=255)

                                               tttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGc                                                                                         <  1:9842754/140‑1 (MQ=255)

                                                ttCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                        >  1:5710645/1‑140 (MQ=255)

                                                gtCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCa                                                                                        <  1:9120686/139‑1 (MQ=255)

                                                 tCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAATGTTAGCTGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCAGCTGACAGCAGAGCAt                                                                                       >  2:8779106/1‑140 (MQ=255)

                                                    aTGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                                                                    <  1:4192369/140‑1 (MQ=255)

                                                    aTGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGt                                                                                    <  2:9004127/140‑1 (MQ=255)

                                                      gCCATCTGCCATCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:948663/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:1857111/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  1:1037941/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:10872986/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  1:8047003/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:5336360/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  1:5170844/140‑1 (MQ=255)

                                                      gCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTa                                                                                  <  2:7500567/140‑1 (MQ=255)

                                                        cATCTGCCAGCAAGCGCACCGATTGCAGGATATTGTTGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                                <  2:10472866/140‑1 (MQ=255)

                                                        cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                                <  1:1592218/140‑1 (MQ=255)

                                                        cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                                <  1:5626064/140‑1 (MQ=255)

                                                        cATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAAt                                                                                <  2:5505441/140‑1 (MQ=255)

                                                         aTCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATg                                                                               >  2:7245915/1‑140 (MQ=255)

                                                           cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                             <  1:2422141/140‑1 (MQ=255)

                                                           cTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGcc                                                                             <  1:4069661/140‑1 (MQ=255)

                                                               cAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcac                                                                         <  2:9746003/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  1:5063490/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  2:2755976/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  1:2844096/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAATTGAGGATCACCATTGGACGGAAGACGTTCACATCAAAGTTACCGGCAGC‑GCCC‑CC‑C‑CTGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  2:8206954/140‑1 (MQ=255)

                                                                aGCAAGCGCACCGATTGCAGGAAAGTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑CTGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTcaca                                                                        <  2:64578/140‑1 (MQ=255)

                                                                  cAAGCGCACCGATGGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACt                                                                      >  1:6034197/1‑140 (MQ=255)

                                                                    aGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACtgt                                                                    >  2:2820074/1‑140 (MQ=255)

                                                                     gcgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑CC‑CC‑CGATGTTGATCGCCACGTCGTTACCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACtgtg                                                                   >  2:9345833/1‑140 (MQ=255)

                                                                      cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                                  >  1:7822692/1‑140 (MQ=255)

                                                                      cgcACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTgtgt                                                                  >  1:1345788/1‑140 (MQ=255)

                                                                        cACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTg                                                                >  1:6281990/1‑140 (MQ=255)

                                                                          ccGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                              <  1:2182311/140‑1 (MQ=255)

                                                                          ccGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTggg                                                              <  1:7594374/140‑1 (MQ=255)

                                                                            gATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGtt                                                            >  1:7112433/1‑140 (MQ=255)

                                                                                gCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTccc                                                                                                            <  1:10268874/88‑1 (MQ=255)

                                                                                     aaaTTGTGTATCACCATGTGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTccc                                                   <  2:5954052/140‑1 (MQ=255)

                                                                                       aTTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCg                                                 >  2:502661/1‑140 (MQ=255)

                                                                                        ttGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCgg                                                <  1:8956484/140‑1 (MQ=255)

                                                                                          gtgGATCACCATTGGACGGAAGACGTTCCGTTCACAGTTACCTGAAGC‑GCCC‑CC‑C‑ATGGTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTGTCCCCGGCa                                              <  2:6329029/140‑1 (MQ=255)

                                                                                           tgGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCAt                                             >  1:4910900/1‑140 (MQ=255)

                                                                                            ggATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATg                                            >  2:316538/1‑140 (MQ=255)

                                                                                             gATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGa                                           <  2:5819436/140‑1 (MQ=255)

                                                                                             gATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGa                                           <  2:658966/140‑1 (MQ=255)

                                                                                              aTCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGAt                                          >  2:4754993/1‑140 (MQ=255)

                                                                                                  ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTgg                                                               >  1:3833013/1‑115 (MQ=255)

                                                                                                  ccATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑CC‑CCAC‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTGTCCCCGGCATGATTGAg                                      <  2:920555/140‑1 (MQ=255)

                                                                                                          cGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCgg                              >  2:2542774/1‑140 (MQ=255)

                                                                                                             aaGACGTTCCGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  2:4011991/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTTGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  2:430892/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  1:9454012/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  2:3038397/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  1:8653247/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  2:7701977/1‑140 (MQ=255)

                                                                                                             aaGACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTc                           >  1:44057/1‑140 (MQ=255)

                                                                                                               gACGTTCAGTTCAAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCAt                         >  1:2060480/1‑140 (MQ=255)

                                                                                                                   ttcagttcaAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTc                     >  1:3334873/1‑140 (MQ=255)

                                                                                                                   ttcagttcaAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTc                     >  2:4149387/1‑140 (MQ=255)

                                                                                                                          caAAGTTACCGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGAtt              <  1:5348762/140‑1 (MQ=255)

                                                                                                                                  ccGGAAGC‑GCCC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTc      >  2:6837861/1‑140 (MQ=255)

TCGCTCACGATTCGGTTCAATACCCACTGCGCAGTGTTTGTTAAAACTTTCCATGCCATCTGCCAGCAAGCGCACCGATTGCAGGAAATTGTGGATCACCATTGGACGGAAGACGTTCAGTTCAAAGTTACCGGAAGC‑G‑CC‑CC‑C‑ATGTTGATCGCCACGTCGTTCCCCATCACCTGACAGCAGAGCATGGTTAATGCCTCACACTGTGTTGGGTTCACTTTCCCCGGCATGATTGAGCTGCCCGGCTCATTTTCCGGGATTGAGATTTCACCA  >  NZ_CP025268/1690217‑1690489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`