BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,826,683	(A)_7→8	intergenic (‑247/+51)	chbB ← / ← osmE	PTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,826,676	1	.	A	94.0%	2776.4 / 141.0	770	intergenic (‑240/+58)	chbB/osmE	PTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE
Reads supporting (aligned to +/- strand): ref base . (38/8); new base A (474/248); total (514/256)
Fisher's exact test for biased strand distribution p-value = 2.28e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 789 total aligned reads displayed.

GTGGCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826538‑1826810

          ggAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑a‑aaaa‑a‑aaccg                                                                                                                              <  1:2184189/140‑5 (MQ=255)

                      ataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCg                                                                                                                  >  2:3925447/1‑140 (MQ=255)

                        aGACCTGAGCAATAGTATTTCTTTGACCTTCTAAATTGTTTCTCCTTGACCTGTGGTTCTGACCCTCTTCACATTTCGGGCAAATAATTCGAGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAA‑A‑ATCCGGCGCAATGGCCGGt                                                                                                                >  2:6454415/1‑140 (MQ=255)

                        aGACCTGAGCAATAGTATTTCTTTGACCTTATATATTGTTTCTCCTTGAACTGTGGTTATGACCCTCTTCAAAATTCGGGCAAATAATTAGCGAAGATCATTAAGAACTCAGGC‑C‑‑A‑AAAA‑A‑ATCCGGCGCAATGGCCGGt                                                                                                                >  2:8969778/1‑140 (MQ=255)

                        aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGt                                                                                                                >  1:10642069/1‑140 (MQ=255)

                        aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGt                                                                                                                >  1:1848219/1‑140 (MQ=255)

                        aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGt                                                                                                                >  2:6625277/1‑140 (MQ=255)

                        aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCGTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAA‑ATA‑CCGGCGCAATGGCCGGt                                                                                                                >  2:7275688/1‑140 (MQ=255)

                        aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTATCTCCTTGACCTGTGGTTATGACCCTCTACACATTTAGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGt                                                                                                                >  1:10002134/1‑140 (MQ=255)

                        aGACCTGAGCAAGAGGATTTCTTTGACCGTATCAAGTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCACATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGt                                                                                                                >  2:3247944/1‑140 (MQ=255)

                          aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:7026852/1‑140 (MQ=255)

                          aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  2:5870849/1‑140 (MQ=255)

                          aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:5939591/1‑140 (MQ=255)

                          aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:802711/1‑140 (MQ=255)

                          aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:7344218/1‑140 (MQ=255)

                          aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:7244803/1‑140 (MQ=255)

                           ccTGAGCAAGAGTATTTCTTTGACCTTATAACTTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTc                                                                                                             <  2:1090717/140‑1 (MQ=255)

                               aGCACTAGTATTTCTTTGACCTAATACATTGTTTCTCCTTGACCTGTGGTTAGGACCCTCTTCACAGTTCGGGCAAATAATTCGCGAAGCGACTTAAGAACTCAGGC‑C‑‑ATAAAA‑A‑A‑CCGGCGCTAGTGCCGGTTTCCgtt                                                                                                         >  2:2996064/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCCAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAATA‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:8260709/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:8705898/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:8801073/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:9224486/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:1059382/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:4998673/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:4405160/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:4242591/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:3965496/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:4694951/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:3407416/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:2435388/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:8730846/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:920673/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:9408905/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:10285438/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑a‑aaaa‑a‑aaccgg                                                                                                                             >  1:7126016/1‑115 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAA‑ATA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:981585/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAA‑A‑ATCCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:6613949/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGAGAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCggt                                                                                                         >  1:6524222/1‑138 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACATGTGGTTATTACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAAGTAAGAACTCAGGC‑C‑‑A‑AAAA‑A‑ATCCGGCGCAATGGCCGGTGTCCgtt                                                                                                         >  2:5999207/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAAGTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAA‑ATA‑CCGGCGCAATGGCCGGTTTCGgtt                                                                                                         >  1:9846762/1‑140 (MQ=255)

                               aGCAATAGTATTTCTTTGACCTTATAAAGTGTTTCTCATTGACCGGTGTTTATGACCCTCTTCACATTTCGGGCAATTAATTCGCGAAGATAATTATGAACTCAGGG‑C‑‑A‑AAAA‑ATA‑CCGGCGCAATGGCCGGTGTCCggt                                                                                                         >  2:7101718/1‑138 (MQ=255)

                                         tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                               >  1:4103269/1‑140 (MQ=255)

                                         tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                               >  2:10342493/1‑140 (MQ=255)

                                           tCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                             <  2:10056156/140‑1 (MQ=255)

                                           tCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAATA‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCt                                                                                             <  2:5781174/140‑1 (MQ=255)

                                              ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTc                                                                                          >  1:3165823/1‑140 (MQ=255)

                                              ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTc                                                                                          >  1:8381743/1‑140 (MQ=255)

                                                gACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAg                                                                                        >  2:1851425/1‑140 (MQ=255)

                                                 aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGa                                                                                       <  1:1049466/140‑1 (MQ=255)

                                                 aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATAAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGATTCCGTTGATACTCAAGATTACAGa                                                                                       >  2:9289148/1‑140 (MQ=255)

                                                  ccTTATAACTTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  2:8558847/140‑1 (MQ=255)

                                                  ccTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAc                                                                                      <  2:2712271/140‑1 (MQ=255)

                                                      ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                                  >  1:3841100/1‑140 (MQ=255)

                                                      ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                                  >  2:4898344/1‑140 (MQ=255)

                                                       taAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAtt                                                                                 <  1:9108323/140‑1 (MQ=255)

                                                             gTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTGTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTAc                                                                           >  1:4375890/1‑140 (MQ=255)

                                                              tttCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACt                                                                          >  2:2483505/1‑140 (MQ=255)

                                                              tttCTCCTTGACCAGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTAATCAAGCTTTCAGACGAATTGATTACt                                                                          >  1:5862965/1‑140 (MQ=255)

                                                                tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCACTGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGAGGATTTGATttcttc                                                                        >  1:7759582/1‑140 (MQ=255)

                                                                tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  1:3695206/1‑140 (MQ=255)

                                                                 ctctTTTACTTGTGTTAATGACCTTCTTAACATTTCGGGCAATTATTTGGCTATTATAATTAAGAAATCAGGCAA‑‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  2:6204973/140‑1 (MQ=255)

                                                                 ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCACGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  1:6900628/140‑1 (MQ=255)

                                                                  tcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGAAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGc                                                                      <  1:4612602/140‑1 (MQ=255)

                                                                   ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:8522810/140‑1 (MQ=255)

                                                                   ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  2:7448095/140‑1 (MQ=255)

                                                                        aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                >  2:5359436/1‑140 (MQ=255)

                                                                             tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATACTTATGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAAAGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTggct                                                           >  2:6748919/1‑138 (MQ=255)

                                                                             tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           <  2:1595610/140‑1 (MQ=255)

                                                                             tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           >  2:3449100/1‑140 (MQ=255)

                                                                             tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           >  1:7466106/1‑140 (MQ=255)

                                                                                  aTGACCCTCTTCAGATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGCTCAgtg                                                      >  1:9395721/1‑140 (MQ=255)

                                                                                  aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  2:10457979/1‑140 (MQ=255)

                                                                                  aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      >  1:1300865/1‑140 (MQ=255)

                                                                                  aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      <  2:5290860/140‑1 (MQ=255)

                                                                                    gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  2:4211331/140‑1 (MQ=255)

                                                                                    gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:5471616/140‑1 (MQ=255)

                                                                                    gACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    >  1:491933/1‑140 (MQ=255)

                                                                                         tctTCACATTTCGGGCAAATAATTCGCGAAGAAACTTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTAtt                                               <  1:9510259/140‑1 (MQ=255)

                                                                                          ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  1:566428/1‑140 (MQ=255)

                                                                                          ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  2:96339/1‑140 (MQ=255)

                                                                                             cacaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGc                                           >  1:1435560/1‑140 (MQ=255)

                                                                                              acttttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:2773885/137‑1 (MQ=255)

                                                                                              acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:5560624/140‑1 (MQ=255)

                                                                                              acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:3575101/140‑1 (MQ=255)

                                                                                                aTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CAAA‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGcac                                         >  2:2363144/1‑140 (MQ=255)

                                                                                                aTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGcaca                                        <  2:292310/140‑1 (MQ=255)

                                                                                                   tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGt                                     <  1:3276557/140‑1 (MQ=255)

                                                                                                   tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGt                                     <  1:5345234/140‑1 (MQ=255)

                                                                                                    cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCGGTGGATCAGTGTCGTATTCAGCACAGGTc                                    >  2:1361894/1‑140 (MQ=255)

                                                                                                     gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                   <  1:258682/140‑1 (MQ=255)

                                                                                                            taatTCGCGAAGATAATTAAGAACTCAGGC‑CAAA‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAAc                             <  2:7676737/140‑1 (MQ=255)

                                                                                                                 cgcgAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGt                       >  1:295818/1‑140 (MQ=255)

                                                                                                                    gAAGATAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGCCCGGTAACCGTAGTGACACAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGGCGTATTCAGCACAGGACAGATAACCGGAGTtga                    >  2:3792840/1‑140 (MQ=255)

                                                                                                                        aTAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:163678/140‑1 (MQ=255)

                                                                                                                        aTAATTAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  2:10692533/140‑1 (MQ=255)

                                                                                                                             tAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  1:1084800/140‑1 (MQ=255)

                                                                                                                             tAAGAACTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTc           <  2:692595/140‑1 (MQ=255)

                                                                                                                                 aaCTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGt       >  2:8326960/1‑140 (MQ=255)

                                                                                                                                  aCTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  2:7974840/1‑140 (MQ=255)

                                                                                                                                  aCTCAGGC‑CA‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  1:6244505/1‑140 (MQ=255)

GTGGCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGC‑C‑‑A‑AAAA‑A‑A‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826538‑1826810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`