BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,466,923	(C)_8→9	intergenic (+242/‑123)	fadL → / → CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,466,915	1	.	C	96.3%	808.2 / 22.7	243	intergenic (+234/‑131)	fadL/CXP41_RS12955	long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand): ref base . (0/9); new base C (42/192); total (42/201)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 322 total aligned reads displayed.

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGC  >  NZ_CP025268/2466778‑2467049

ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc                                                                                                                                       <  1:4177518/140‑1 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGATAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑c                                                                                                                                     <  1:1975130/140‑1 (MQ=255)

 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑c                                                                                                                                     <  1:2378188/140‑1 (MQ=255)

  gCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑cc                                                                                                                                    <  1:2649137/140‑1 (MQ=255)

   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAACTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccc                                                                                                                                   <  2:4046770/140‑1 (MQ=255)

   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccc                                                                                                                                   >  1:9233515/1‑140 (MQ=255)

   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccc                                                                                                                                   <  1:7957455/140‑1 (MQ=255)

   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccc                                                                                                                                   <  1:7566127/140‑1 (MQ=255)

   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccc                                                                                                                                   <  2:963485/140‑1 (MQ=255)

    aaaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGATTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑cccc                                                                                                                                  <  1:7216495/140‑1 (MQ=255)

     accAGCCTGTTTGCAGCTTGCCCATTCAAATCAATAAGCGTTATCCACAGACAAAACTTTATGGAAAGGCATATGTTCAGGCGTTCTGATCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccc                                                                                                                                 <  2:8338399/138‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccc                                                                                                                                 <  1:6813209/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccc                                                                                                                                 <  2:121162/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccc                                                                                                                                 <  2:2384952/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccc                                                                                                                                 <  1:2536653/140‑1 (MQ=255)

     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTACTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccc                                                                                                                                 <  1:59867/140‑1 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCGGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccccc                                                                                                                               <  1:3545717/140‑2 (MQ=255)

       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccccc                                                                                                                               <  1:1002763/140‑2 (MQ=255)

        aGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccccct                                                                                                                              >  1:2166184/1‑138 (MQ=255)

          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGCCAAACCTGAAGTGAAAGGCATATTTTCAGTCGTTCTGCTCGTCCTTCTCAAAGAGTTTAATTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccccctgg                                                                                                                            <  2:7210978/140‑5 (MQ=255)

          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccccc                                                                                                                               <  1:1281142/137‑2 (MQ=255)

          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccccc                                                                                                                               >  2:1281142/1‑136 (MQ=255)

          ccTGTTGGCAGCTTGCCCTTTCAAATCAAGAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑cc‑ccccccctgg                                                                                                                            <  2:2197741/140‑5 (MQ=255)

          ccTGTTGGCAGCTGGCCCAATCATATCAATCCGCGGTCTCCACCGACAAACCCGAAGGGAAAGGCCTACTCTCAGGCGTTCTGCTCGTCCGTCTCCACGAGTTGACTTGGCGGCCTTTCCACCAT‑AC‑T‑‑cc‑ccccccctgg                                                                                                                            <  2:1898369/140‑5 (MQ=255)

             gTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTa                                                                                                                          <  1:5663554/140‑1 (MQ=255)

               tGGCAGCTTGCCCTTTCAAATCACTCAGCGTTATCCACAGACTAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTGTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTAtt                                                                                                                        <  2:6064522/140‑1 (MQ=255)

                gtcAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACTGACTAACCTGAAGGGAAAGGCTTATTTTCAGGCGTTCTGCTCTTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtg                                                                                                                       <  2:3589846/138‑1 (MQ=255)

                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCTTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtg                                                                                                                       <  2:4221498/140‑1 (MQ=255)

                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtg                                                                                                                       <  2:7277426/140‑1 (MQ=255)

                  caccTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGCAGGGAAAGGCATATTTACAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtgtg                                                                                                                     <  2:8165454/137‑1 (MQ=255)

                  cAGCTTGCCCTTTCACATCCATAAGCGGTATCCACAGACAAACCTGAAGGGACAGGCATATTTTCCGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtgtg                                                                                                                     <  2:4351295/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACCGACAAACCGGAAGGTAAAGGCATATTTACAGGCGTTCGGCTCGTCCTTCTCAACGAGTTTACTTTCCTGCATTTCCAGGATAAC‑C‑‑CC‑CCCCCCTGGCTATtgtg                                                                                                                     <  2:5215633/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACATACAAACCTGAAGTGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtgtg                                                                                                                     <  1:4000100/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCACAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtgtg                                                                                                                     <  1:2845775/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtgtg                                                                                                                     <  1:4098796/140‑1 (MQ=255)

                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATtgtg                                                                                                                     <  1:7291090/140‑1 (MQ=255)

                  cAGCTTGCCCTTTAAAATCAATAAGCGTTATCCACAGACAAACCTGATGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑CCACCCCCCTGGCTATtgtg                                                                                                                     <  2:8217449/140‑1 (MQ=255)

                  cAGCTTGCCCTTGCAAAGCAATACGCGGGATCCACAGACAACCCTGCAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCACAGAGTTTCCTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCGGGCTATggtg                                                                                                                     <  2:6453292/140‑1 (MQ=255)

                  aaGCTTGCCCTTTCAAATCAATATGCGGTATCAACATACAAACATGAAGGGAAAGGAATATTTTATGGCGTTCTGCTCGTCCTTATAAAATATTTTACTTTTATGAATTTCAAGGAT‑AA‑TC‑CC‑CCCCCCTGGCTATtgtg                                                                                                                     <  1:10715202/139‑1 (MQ=255)

                   aGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTgc                                                                                                                    <  1:6656057/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTgcg                                                                                                                   <  2:1353371/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTgcg                                                                                                                   <  1:4763504/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTgcg                                                                                                                   <  1:7006495/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTgcg                                                                                                                   <  1:9511384/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTgcg                                                                                                                   <  1:8866778/140‑1 (MQ=255)

                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGATTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTgcg                                                                                                                   <  2:5719945/140‑1 (MQ=255)

                     cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCGATTGTgcgc                                                                                                                  >  1:3595036/1‑140 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCa                                                                                                               <  2:7109159/140‑1 (MQ=255)

                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCa                                                                                                               <  1:5495139/140‑1 (MQ=255)

                         cccTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCAt                                                                                                              <  2:1609857/140‑1 (MQ=255)

                            tttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCACATAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATaca                                                                                                           <  2:6831844/140‑1 (MQ=255)

                            tttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATaca                                                                                                           <  1:9173322/140‑1 (MQ=255)

                               cAAATCACTAAGCGGTATCCACAGACAAACCTGAAGCGCCTCGCAAATTTTAAGGCGTTCTGCTCGTCCTTCTACTAGAGTATACTATTCAGCATTTCCAGGTT‑AC‑TC‑CC‑CCCCCCGGGCTATTGGGCGCTCAAACACTc                                                                                                        <  2:7477273/140‑1 (MQ=255)

                                          gcgcgaTCCCCAGACACACCTCACGGGAAAGGCATCTTTTCCGGCCGTCTGCGCTTCCATCTCCAACAGTGTACTTTTCTCCAGTTCCAGGAT‑ACGC‑‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCCAAATACAGAt                                                                                             <  2:10236946/135‑1 (MQ=255)

                                          gCGGTATCCACAGCCAAACCTGACGGGAAAGGCATATTTACAGGCGTTCTGCACGACCTTCTCACAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCGGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                             <  1:1645723/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGATAGGCATATATTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                             <  2:5000297/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                             <  1:2080196/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                             <  1:1148393/140‑1 (MQ=255)

                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                             <  1:6757711/140‑1 (MQ=255)

                                          gCGGTATCCACAGAAAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                             <  2:4592985/140‑1 (MQ=255)

                                            ggTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAg                                                                                           >  2:10037433/1‑140 (MQ=255)

                                             gTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                          <  2:3399017/140‑1 (MQ=255)

                                             gTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                          <  1:1937539/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                        <  1:6849722/140‑1 (MQ=255)

                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                        <  2:9028103/140‑1 (MQ=255)

                                                     aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTa                                                                                                                          <  1:6321765/100‑1 (MQ=255)

                                                     aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCGGGCGAGTGTGCGCGCCGACACTCAAAGTAACGATAGGTTCtaaat                                                                                  >  1:1412039/1‑140 (MQ=255)

                                                       aCAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataa                                                                                >  1:778282/1‑140 (MQ=255)

                                                        cAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataaa                                                                               >  1:10507461/1‑140 (MQ=255)

                                                            ccTGAAGGGAAAGGCATATTTTCATGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAATTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                           <  2:3508376/140‑1 (MQ=255)

                                                               gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGt                                                                        >  1:3372426/1‑140 (MQ=255)

                                                                     aaaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                  >  2:7655616/1‑140 (MQ=255)

                                                                      caGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                 <  1:8695963/139‑1 (MQ=255)

                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                                 <  1:10819109/140‑1 (MQ=255)

                                                                        ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                               <  1:6511553/140‑1 (MQ=255)

                                                                        ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                               <  1:9946856/140‑1 (MQ=255)

                                                                        ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGATTTTTGATa                                                               <  2:476293/140‑1 (MQ=255)

                                                                         gCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                                                              <  1:5163776/140‑1 (MQ=255)

                                                                         gCATATGTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATACATGAGCGTTTTTTGATAg                                                              <  2:8822611/140‑1 (MQ=255)

                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                          <  1:9622878/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                     <  2:3149121/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                     <  2:2689103/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                     <  1:8063861/140‑1 (MQ=255)

                                                                                  cAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                     <  1:7860733/140‑1 (MQ=255)

                                                                                   agtcGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                    <  2:10371486/137‑1 (MQ=255)

                                                                                   agtcGTTCATCTCGTCCTTATCAAAGAGATAACTTTTCTGCATTTACTGGAT‑AC‑TC‑CC‑CCCCCCTGGCTTTTGTGCGCTCATACCATCAAATTCAAGAACGGGTCTAAATAAATGACCGATTTTTGATAGTCTATTTCAt                                                    <  2:6199242/137‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                    <  1:10117322/140‑1 (MQ=255)

                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                    <  2:20403/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                   <  1:7487431/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                   <  2:8518781/140‑1 (MQ=255)

                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                   <  1:3816158/140‑1 (MQ=255)

                                                                                     gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TCCCC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                   <  1:4402456/140‑1 (MQ=255)

                                                                                     gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa                                                  <  1:1820269/140‑1 (MQ=255)

                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                              <  1:2345360/140‑1 (MQ=255)

                                                                                         tCTGCTCGTCCTTCTAAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                              <  2:5466444/140‑1 (MQ=255)

                                                                                                     tctcAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGt                                  >  2:9301075/1‑140 (MQ=255)

                                                                                                     tctcAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCGATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGt                                  >  2:3025392/1‑140 (MQ=255)

                                                                                                                         tGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTCTTGTGCGCTCATACACTCAAATTAAATATAGGTTCTAAATAAATGAGCGTTTTTTGATATTCTATTTCATTAGTTAATATATATTTGTAACAAATCAATAAAAATTGa              >  1:8444147/1‑140 (MQ=255)

                                                                                                                         tGCATTTCCAGGAT‑AC‑TC‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAtttaa                                                     >  2:753853/1‑99 (MQ=255)

                                                                                                                               tCCACGAC‑AC‑TC‑CC‑CCCCCCTGTCTTGTGTGCGATCATCAACTCAAATTAAAGATAGTTTCTAAATAAATGAGCGTTTTATGAGAGACTATTTCATTAGGTAATATATAATTGTAACAAATCAATCCAAAGGGAATaaaa        <  2:9661307/140‑1 (MQ=255)

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AC‑T‑‑CC‑CCCCCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCATGC  >  NZ_CP025268/2466778‑2467049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`