BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,769,852	+C	intergenic (‑52/‑89)	yfjM ← / → rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,769,848	1	.	C	96.3%	1693.2 / 51.3	513	intergenic (‑48/‑93)	yfjM/rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA
Reads supporting (aligned to +/- strand): ref base . (6/13); new base C (219/272); total (225/288)
Fisher's exact test for biased strand distribution p-value = 3.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 541 total aligned reads displayed.

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NZ_CP025268/2769710‑2769982

 cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑cc                                                                                                                                        <  1:7784997/140‑1 (MQ=255)

 cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑cc                                                                                                                                        <  1:9150276/140‑1 (MQ=255)

 cTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑cc                                                                                                                                        <  1:2054205/140‑1 (MQ=255)

 cTTGTATGCTGGCGTATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTTCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑cc                                                                                                                                        <  1:632262/140‑1 (MQ=255)

   tGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGTTCCTGCCGTATAGTTGGAGTG‑cc‑cc                                                                                                                                     >  1:7396916/1‑140 (MQ=255)

            gCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAgg                                                                                                                            <  2:454796/140‑1 (MQ=255)

               aataatTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTgg                                                                                                                         <  1:1319500/140‑1 (MQ=255)

                    ttACTTAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACTTCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTaaa                                                                                                                    <  2:9537438/140‑1 (MQ=255)

                    ttACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTaaa                                                                                                                    <  1:10006644/140‑1 (MQ=255)

                          aGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGc                                                                                                              >  1:8292042/1‑140 (MQ=255)

                           gTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCa                                                                                                             <  2:10078561/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTCGACGATCCTGCCGTATCGTGGGAGTGCCC‑CC‑GAGTCAGGTGGCTACATTTAGCacct                                                                                                          >  2:10644344/1‑137 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          >  1:1715009/1‑140 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAAt                                                                                                          <  2:9207399/140‑1 (MQ=255)

                              tCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTGTGTGGACTGCCCCCCCGGTAGACGATCATGCCCTAGAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTCGCAAAt                                                                                                          >  2:1613472/1‑140 (MQ=255)

                                 actcgctgctGGGGGAGCGCAGGAAGACGTGACCGACACGATGGACATCATCACCAAATGTGTGGACTGCACCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGTTGTATAACTTGAGCAAATCac                                                                                                       <  2:9647861/136‑2 (MQ=255)

                                 aGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCaa                                                                                                       <  2:10364969/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:6794825/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:8561635/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:4091774/140‑1 (MQ=255)

                                   ttGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATa                                                                                                     <  1:10546794/140‑1 (MQ=255)

                                   gtgctgcTTGTTGAGAACAGGAAGATGTTACCGAAACGATGTACATCATCCCCCATTTTGTTGACTGCCCCCCCGGTAGACGATCCTGCCCTATATTTGGAGTGCCC‑CC‑TTTTCAGGTGGCTAAATTTAGCAAAAAAATa                                                                                                     <  2:4138470/139‑1 (MQ=255)

                                    tgctgcTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TAGTCAGGTGGCTAAATTTAGCAAATCAATac                                                                                                    >  2:4825336/1‑140 (MQ=255)

                                     gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                                   <  1:7101102/140‑1 (MQ=255)

                                     gctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTGTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATaca                                                                                                   <  1:1622662/140‑1 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TCTTCAGGGGGCTAAATTTAGCAAATCAATacac                                                                                                  >  1:5485272/1‑140 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                  >  2:1287148/1‑140 (MQ=255)

                                      ctgctTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATacac                                                                                                  >  1:2898790/1‑140 (MQ=255)

                                      ctgctTGGAGAGATCAGGAAGTGGTTACCGAAACGATGGACATCATCCCCCATGTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGTGTGCCC‑CC‑TATTCAGGTGGCGAAATTTAGCAAATCAATaccc                                                                                                  >  2:10901530/1‑138 (MQ=255)

                                          ttGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCa                                                                                              <  1:10364621/140‑1 (MQ=255)

                                             gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  2:7555997/140‑1 (MQ=255)

                                             gTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAggg                                                                                           <  1:3305170/140‑1 (MQ=255)

                                              tGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggg                                                                                          >  2:2894032/1‑140 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAGACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  1:5110083/140‑1 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  2:2409285/140‑1 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  2:2228131/140‑1 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        >  2:8564614/1‑140 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  1:4446805/140‑1 (MQ=255)

                                                agaTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCAGTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAgggggg                                                                                        <  2:6790181/140‑1 (MQ=255)

                                                 gctCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTGTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGAGGCTAAATTTAGCAAATCACTACTCTACAGGGGGGt                                                                                       <  2:8862069/138‑1 (MQ=255)

                                                  aTCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGta                                                                                      >  1:4278422/1‑140 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     <  2:9686301/140‑1 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     >  1:4587969/1‑140 (MQ=255)

                                                   tCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTGGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtat                                                                                     >  2:7983554/1‑140 (MQ=255)

                                                         agagGTTACCGAAACGATGGACATCATCCCCCATATTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGt                                                                               <  2:9765157/140‑1 (MQ=255)

                                                          catgTTACCGACAAGATGGAATTCATCCCCCATTTTGTGTACTGCCACCCAGGTAGACGATCCTGCCCTATAGTTGGAGTGACC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGTGGTTTTATTGTa                                                                              <  2:6648253/137‑1 (MQ=255)

                                                             gTTACCGAAACGATGGACCTCATCCCCCATTGTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCGCTAGTTGGAGTG‑CC‑CCATATTCAGGGGGCTAATTTTAGCACATCAATAAACGTCAGGGGGGGATTGTTGTagag                                                                           >  2:8440572/1‑140 (MQ=255)

                                                             gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTCTAGTGGGAGTGCCC‑CC‑TAGTCAGGTGGCTAAATTTAGCAAATCAATCCACTTCAGGGGGGTATTATTGTagag                                                                           >  1:2687929/1‑140 (MQ=255)

                                                             gTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TAGTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTCTTATTGTagag                                                                           >  1:8394892/1‑140 (MQ=255)

                                                               tACCGAAACGTTGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGGAGACGTTCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGTGGGGTATTATTGTAGAGtt                                                                         <  2:4644584/140‑1 (MQ=255)

                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGtattat                                                                                  >  2:3898402/1‑131 (MQ=255)

                                                               tACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGtt                                                                         <  1:8776086/140‑1 (MQ=255)

                                                                aCCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGAGTATTGTAGAGttt                                                                        >  1:9238942/1‑140 (MQ=255)

                                                                 ccGCAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCAGGTATACGATCCTGACCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCACTACACTTCAGGGGGGTATTTTTGTAGAGTTTc                                                                       <  2:7672676/140‑1 (MQ=255)

                                                                  cGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                      <  2:3488567/140‑1 (MQ=255)

                                                                     aaCGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCa                                                                   >  1:10874425/1‑140 (MQ=255)

                                                                       ctaTGTACATCATCCCCCTTTTTGTGTACTGCCCCCCGGGTAGACTATCCTGCCCTTTCGTTGGATTGCCC‑CC‑TATTCTGGTGGATAATTTTTGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                                                                 <  2:4456300/138‑1 (MQ=255)

                                                                       cGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                                                                 <  1:8116774/140‑1 (MQ=255)

                                                                       cGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                                                                 <  1:10596205/140‑1 (MQ=255)

                                                                       cGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCata                                                                 <  1:7308804/140‑1 (MQ=255)

                                                                        gATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGATTCTTGTAGAGTTTCCCCctat                                                                >  2:10507142/1‑140 (MQ=255)

                                                                         aTGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATg                                                               >  1:2110581/1‑140 (MQ=255)

                                                                               atcatcCCCCATTTTTTGGACTGCCCCCACGGTAGACTATCCTGCCCTATAGTTGGAGGGACC‑CA‑TATTCAGGTGGCTAAATATAGCAAATCTATACACTTCAGGGGTGTATTATTGTAGAGGTTCCCCATATGTTTCTa                                                         <  2:8149529/140‑1 (MQ=255)

                                                                               atcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTa                                                         <  1:5256252/140‑1 (MQ=255)

                                                                                tcatcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAAGTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTGTCTAt                                                        <  2:3338709/140‑1 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                       <  1:9956762/140‑1 (MQ=255)

                                                                                 catcCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                                       <  1:8169056/140‑1 (MQ=255)

                                                                                          ttttGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAgg                                              <  1:6121268/140‑1 (MQ=255)

                                                                                            ttGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaa                                            >  1:7048572/1‑140 (MQ=255)

                                                                                              gtgGACTGCCCCCCCGGTAGACGATCCTGCCCTATCGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTGTCTATGGGATCCAGGAAAt                                          >  2:2515712/1‑140 (MQ=255)

                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt                                    >  2:8963083/1‑140 (MQ=255)

                                                                                                    tGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt                                    >  2:5922940/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTcc                                                                      <  1:10231297/104‑1 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCa                                  >  1:3836496/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATACAGGAAATGACAATCa                                  >  2:3621652/1‑140 (MQ=255)

                                                                                                      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTAGGGGAGCCAGGAAATGACAATCa                                  >  2:6508175/1‑140 (MQ=255)

                                                                                                           ccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGt                             <  1:2281462/140‑1 (MQ=255)

                                                                                                            cGGTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGtt                            >  2:7162459/1‑140 (MQ=255)

                                                                                                              gTAGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACACTCAGGAGTTAc                          >  2:7906760/1‑140 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:9634888/140‑1 (MQ=255)

                                                                                                                aGACGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaa                        <  1:5718068/140‑1 (MQ=255)

                                                                                                                 gaatttCCTTCACTATAGGTGGATTT‑CCACC‑TATTCCTGTGGATAACATTAGCAAATCAATACACTTAAGGGGTGTATGATTGTAGAGTTTCCCCATATGTTTCTATTGGATCCAGGAAATGACAATCAGGAGTTACaaa                       <  2:6307476/135‑1 (MQ=255)

                                                                                                                  aCGATCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaaa                      >  1:10716157/1‑140 (MQ=255)

                                                                                                                     aTCCTGCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTCTGTGATACAGGAAATGACAATCAGGAGTTACAAAAACt                   >  2:7205116/1‑140 (MQ=255)

                                                                                                                          gCCCTATAGTTGGAGTGCCC‑CC‑TATTCANGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAt              <  1:5880286/140‑1 (MQ=255)

                                                                                                                          gCCCTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAt              <  1:2564936/140‑1 (MQ=255)

                                                                                                                           cccTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATc             >  1:5567426/1‑140 (MQ=255)

                                                                                                                            ccTATAGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCt            >  2:5452649/1‑140 (MQ=255)

                                                                                                                                tcgTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATGTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCAGATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  2:6019289/138‑1 (MQ=255)

                                                                                                                                taGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:5190147/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  1:3407663/140‑1 (MQ=255)

                                                                                                                                taGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:5741196/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        <  1:4667236/140‑1 (MQ=255)

                                                                                                                                taGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  2:1869819/1‑140 (MQ=255)

                                                                                                                                taGTTGGAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTc        >  1:4849386/1‑140 (MQ=255)

                                                                                                                                     ggAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTATCAAATCAATACTCTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAAGGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  2:1698408/1‑140 (MQ=255)

                                                                                                                                     ggAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:660265/1‑140 (MQ=255)

                                                                                                                                     ggAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:759807/1‑140 (MQ=255)

                                                                                                                                     ggAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc   >  1:8990390/1‑140 (MQ=255)

                                                                                                                                      gAGTGCCC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa  <  1:3580719/140‑1 (MQ=255)

TCTTGTCTGCTGGCGAATAATTACTAAGTATCGAGTTGCTGCTTGGTGAGATCAGGAAGAGGTTACCGAAACGATGGACATCATCCCCCATTTTGTGGACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTG‑CC‑CC‑TATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCA  >  NZ_CP025268/2769710‑2769982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`