Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2260CA21.9% 333.7 / 22.6 187intergenic (+126/+247)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base C (62/84);  new base A (41/0);  total (103/84)
Fisher's exact test for biased strand distribution p-value = 2.78e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 203 total aligned reads displayed.

GATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGG  >  NZ_CP025268/2233088‑2233365
                                                                                                                                           |                                                                                                                                            
gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcg                                                                                                                                             >  2:1881490/1‑140 (MQ=255)
 atgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgc                                                                                                                                            >  2:8450593/1‑140 (MQ=255)
   gatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccg                                                                                                                                          <  1:10061654/140‑1 (MQ=255)
   gatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccg                                                                                                                                          <  1:4821973/140‑1 (MQ=255)
                 aattaatGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATcgccgcc                                                                                                                                        <  1:1912028/128‑1 (MQ=255)
                       tGTCATCAGGACCGAAAAGCACGAGAATATTTCAGTCACTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCggg                                                                                                                      <  2:392347/140‑1 (MQ=25)
                         tcatcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGct                                                                                                                    <  1:5450504/140‑1 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGGCCAA‑AGCCGCCCCCCCCCGCAccccccgcccac                                                                                                                  >  1:9273920/1‑133 (MQ=37)
                            tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCGGCACCCCCGGGCTCTg                                                                                                                 >  2:10593337/1‑140 (MQ=255)
                            tcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCCCCCCCCCCAGCACCCCCCGGCTCTg                                                                                                                 >  2:4384225/1‑140 (MQ=37)
                                  ccGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAAc                                                                                                          <  2:9252105/140‑1 (MQ=255)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCTTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAAcc                                                                                                         >  2:8307173/1‑139 (MQ=255)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGGGGGCCATCGCCGCCCCCCCCCGCACCCCCCGGCGCTGGG‑Gcaca                                                                                                         >  2:2980129/1‑140 (MQ=25)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGGGCCATCGCCCCCGCCCCCACCAACCCCCGGCGCAGGC‑GAACa                                                                                                         >  1:10478946/1‑140 (MQ=25)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGGCCCATCGCCGCCGCCCCCAGCCCCCCCCGGCGCTGGC‑ggcca                                                                                                         >  1:8294809/1‑136 (MQ=25)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAAAGCCCCCCCCCCCAGCACCCCCCCGCGCGGGC‑Gcaca                                                                                                         >  1:8027454/1‑140 (MQ=25)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGC‑GAACa                                                                                                         >  2:10227605/1‑140 (MQ=255)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCAACGCCGCCGCCCCCAGCACCCCCCGGCTCTGGC‑GAACa                                                                                                         >  2:10337700/1‑140 (MQ=255)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAACGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GGACaaa                                                                                                       >  2:6514779/1‑140 (MQ=255)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTGCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCATAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGC‑GAACaaa                                                                                                       >  2:2551588/1‑140 (MQ=25)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGGGGCCAAAAGCCCCCGCCCCCAGCACCCCCCCGCTCAGGG‑GGACaaaaaaac                                                                                                  >  1:792825/1‑136 (MQ=25)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAACCCCCCGCCCCCAGCACCCCCCGGCACTGGC‑GACAaaaaaagc                                                                                                  >  2:5908325/1‑136 (MQ=25)
                                           aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGGGGGGGGGGCCCAACGCCGCCCCCCCCACCCCCCCCCCGCACAGGCGGAACaaaaaccc                                                                                                  >  1:4502308/1‑136 (MQ=255)
                                              agaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCt                                                                                              <  1:4671199/140‑1 (MQ=255)
                                               gaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCACGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCTGGCTCTGGC‑GAACAAAATCGCCGCTg                                                                                             <  2:4686239/140‑1 (MQ=255)
                                                   atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCTTGTGCATTGCTTCTTATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGcgcg                                                                                         <  2:409516/140‑1 (MQ=255)
                                                   atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGcgcg                                                                                         <  1:10267111/140‑1 (MQ=255)
                                                   atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGGGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGcgcg                                                                                         <  1:8527731/140‑1 (MQ=255)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                  >  2:9836804/1‑140 (MQ=255)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                  <  1:7017774/140‑1 (MQ=255)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCATCAACCCCCGGCTCAGGC‑GAAAAAAAACGCAGCAGCCCCGTGccca                                                                                  >  2:7209172/1‑139 (MQ=18)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCGCCCCCTGCACCCCCCGGCTCAGGC‑GAACAAAAAAGCCCCTGCGCGGTGccca                                                                                  >  2:3501015/1‑139 (MQ=18)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCCCCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                  >  2:701485/1‑140 (MQ=255)
                                                              tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGc                                                                              <  1:4182126/140‑1 (MQ=255)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGCGGCCAATCGCCGCCCCCCCCTGCAACCCCGGGCTCTGGC‑GAACAAAATAGCCGCTGCGCGGTGCCCTCGGCt                                                                             >  2:2815916/1‑140 (MQ=255)
                                                                aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCtt                                                                            <  1:10337700/140‑1 (MQ=255)
                                                                aTCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCtt                                                                            <  2:1505567/140‑1 (MQ=255)
                                                                 tCCTGGTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                           <  1:1227870/140‑1 (MQ=255)
                                                                      ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCGTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCt                                                                      >  2:10386968/1‑140 (MQ=255)
                                                                      ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGCGCATGC‑GAACCAAAAAGCCGCTGCGCGGTGGCCCCGGCCTATacca                                                                      >  2:4536818/1‑139 (MQ=18)
                                                                           cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGTGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                                 <  1:2051628/140‑1 (MQ=255)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCCAACCCCGCCGCCCCCCGCACCCCCCGGCGCAGGC‑GCACAAAAACACCGCCGCGCGGTGCCCACGGCGTATCCCATACCGCTACCCGGTCg                                                      >  2:3647287/1‑140 (MQ=14)
                                                                                        cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCTGCCAATATCCCCCCCCCCCGGCACCCCCCGGCGCAGGC‑GAACAAAAAATCCCCGGCGCGGTGCCCACCGCGTAAACCATAAAGCTACCCCGTCggg                                                    >  2:1381243/1‑140 (MQ=9)
                                                                                        cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGGCCATACCCCCCCCCCCCATCACCCCCCCGCTCAAGC‑GAAAAAAAACTCCGCTGCGCGGTGGCCAAGGCTTATAACTAACCGCTACCCGGTAggg                                                    >  2:6821468/1‑140 (MQ=9)
                                                                                        cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                    <  2:5100206/140‑1 (MQ=255)
                                                                                          ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                  <  1:7209172/140‑1 (MQ=255)
                                                                                          ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                  <  1:3769317/140‑1 (MQ=255)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCGGGGTGGGGCCAAACGCCGCCGCCCCCAGCACCCCCGTGCTATGGC‑GCACAAAATCGCAGGTGCGCGGGGGCCTCGGCTGTTACCATTCGGCTTCCGGGGCGGgggc                                                 >  2:7879106/1‑140 (MQ=9)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                 <  2:8199471/140‑1 (MQ=255)
                                                                                             cTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCCGCTAATGC‑GCAAAAAAACGCCCCGTGGCGGTGCCCACGGCGTATACCCTACGGCAAACAGGGAGGGcgcgc                                               >  2:3200435/1‑139 (MQ=9)
                                                                                             cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGGCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGC‑GAAAAAAATAGACTCTGAGCGGTGCCCTCGGGGTATCCCGGAAGGCTCACGTGTCGGGcgcgg                                               >  2:6415083/1‑139 (MQ=14)
                                                                                             cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                               <  2:3748933/140‑1 (MQ=255)
                                                                                               tCATATAATCACTGGCGCAAGGCGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAgg                                             >  2:8296009/1‑140 (MQ=255)
                                                                                                cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCCATCGCCGCCCCCCCCAGCACCCCCCGGCGCTTGA‑GAACAAAAAACCCGCCGCGCGGTGCCCACCGCGTATCCCAAACGGCGAACCGGGCGGGCGCGACGt                                            >  2:5211112/1‑140 (MQ=9)
                                                                                                cATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCAGCGCGGTGCCCTCGGCATAACCCTTACGGCGACCGGGTCGGGCGCGTGGt                                            >  2:6446747/1‑140 (MQ=14)
                                                                                                  tataATCACTGGCGCAAGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                          <  2:158071/140‑1 (MQ=37)
                                                                                                  tataATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                          <  2:3582726/140‑1 (MQ=37)
                                                                                                   ataATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAAACCCGCCGCCCCCAGCACCCCCCGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCACGGCTTATACCATACGGCTACCCGGTCGGGCGCGAGGTAAc                                         >  2:8927042/1‑140 (MQ=11)
                                                                                                     aaTCACTGGCGCAAGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                       <  1:3103725/140‑1 (MQ=21)
                                                                                                      aTCACTGGCGCAAGGAGCGC‑GCCGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGGGCCCTCGGCTTTTCCCTTACGGCGACCGGGGCGGGCGCGCGGGAAccgc                                      >  2:1800463/1‑137 (MQ=11)
                                                                                                       tCACTGGCGCATGGAGCGC‑GCTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                     <  1:4909784/140‑1 (MQ=18)
                                                                                                       tCACTGGCGCATGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                     <  1:2980129/140‑1 (MQ=18)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATAGCCGCCGCCCCCAGCCCCCCCGGGCTCTGGC‑GAAAAAAATAGCCGCTGCGCGGTGCCCACGGCGTATACCTTAAGGCTACCGGGTCGGGCGCGAGGGAACATcc                                     >  2:5744643/1‑140 (MQ=11)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGCCGCCCAAACCCCCCCCCCCCAGCACCCCCCCGCTCAGGC‑GAACAAAAACGCCGCATCGCGGTGCCCACGGCATATCCCAAAACGCTAACCGGTCCGGCGCCAGGTAACATcc                                     >  2:6054732/1‑140 (MQ=2)
                                                                                                          cTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTg                                  <  1:6054732/140‑1 (MQ=32)
                                                                                                          cTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTTGC‑GAAAAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCATACGGCTACCCGGTCGGGCGCGAGGTAACaaaccag                                  >  2:10002905/1‑134 (MQ=11)
                                                                                                           tGGCGCAAGGAGCGC‑GCAGGGGGGGGCCAAAAGCCCCCCCCCCCCGCACCCCCCGGCTCTGGC‑GAACAAAAAACCCCCAGCCCGGGTCCCACCGCTTAACCCATAACGCTACCCTGTCGGGCGCGAGCTAACATcccact                                 >  2:743997/1‑137 (MQ=2)
                                                                                                           tGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCATCACCCCCCGGCTCTGGC‑GAACAAAAACGCCGCTGCGCGGTGCCCACCGCTTATCCCATACGGCTACCCGGTCGGGCGCGAGGTAACaaaccagt                                 >  2:790445/1‑133 (MQ=11)
                                                                                                            ggCGCAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCCCCCCCAGCACCCCCCGGCGCTGGC‑GAACAAAATAGCCGCAGCGCGGTGCCCACGGCGTATACCTTACGGCAAACCGGTCGGGCGCGCGGTAACAACCCAGTa                                >  2:915250/1‑140 (MQ=11)
                                                                                                             gcgcAAGGAGCGC‑GCAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGC‑GCACAAAATACCCGCCGCGCGGTGCCCACGGCTTATCCCGTAACGCTACCCGGTCGGGCGCGAGGTAACAAACCAGTaa                               >  2:8656759/1‑140 (MQ=11)
                                                                                                                 aaGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                           <  2:677017/140‑1 (MQ=32)
                                                                                                                    gAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                        <  1:3302321/140‑1 (MQ=32)
                                                                                                                       cgc‑gcCGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                     <  1:5316675/140‑1 (MQ=17)
                                                                                                                       cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                     <  1:10013362/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                     <  2:10723439/140‑1 (MQ=32)
                                                                                                                         c‑gcAGGGGGCGGCCAATCGCCGCCCCCCCCAGCACCCCCCGGCTCTGGC‑GAACAAAATCGCCGCGGCGCGGTGCCCACGGCGTATACCGTACGGCTACCGGGTCGGGCGCGAGGTAACAACCCAGTAAAACGCGCCcaca                   >  2:1938926/1‑140 (MQ=11)
                                                                                                                          cggaGGGGGCGGCCCATCGCCCCCGCCCCCTGCACCCCCCGGCTCTGGC‑GCACAAAATCGCCGCTGCGCGGGGACCGCGGCGTATACCTTAAGGCTCCCCGGTGGGGCGCGGGGTAACCTCCCAGTAAAAAGCGCCcaca                   >  2:10475340/4‑140 (MQ=2)
                                                                                                                            ctgggggCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                 <  1:5075946/138‑1 (MQ=17)
                                                                                                                            cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                 <  2:8760627/140‑1 (MQ=32)
                                                                                                                             aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                <  1:840395/140‑1 (MQ=32)
                                                                                                                             aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                <  2:8046214/140‑1 (MQ=32)
                                                                                                                             aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                <  2:792825/140‑1 (MQ=32)
                                                                                                                             aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                <  2:5450688/140‑1 (MQ=32)
                                                                                                                               ggggCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCca              <  1:8927042/140‑1 (MQ=32)
                                                                                                                                 ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca            <  2:3043685/140‑1 (MQ=32)
                                                                                                                                 ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca            <  1:5213243/140‑1 (MQ=32)
                                                                                                                                 ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca            <  2:10870095/140‑1 (MQ=32)
                                                                                                                                 ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca            <  2:10890754/140‑1 (MQ=32)
                                                                                                                                 ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca            <  1:4536818/140‑1 (MQ=32)
                                                                                                                                 ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca            <  1:4022917/140‑1 (MQ=32)
                                                                                                                                 ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGATTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca            <  2:8346049/140‑1 (MQ=17)
                                                                                                                                    ggcCAATCGCCGCCGCCCCCTGCACCCCCGGGGTCTGGC‑GACCAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCGGTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTACAACGCGCCCTCAGCCcagagcc         >  2:5390312/1‑135 (MQ=11)
                                                                                                                                     gcCAATCGGCGCCGCCCCCTGCACCCCCGGGCTCTTGC‑GAACAAAATCGCCGCTGCGCGGGGCCCTCGGCGAATACCATAAGGCAACCTGTTACGGCGCGAGGTTACAACCCTGTAAAACGCGCCCTCAACCCACCTCCa        >  1:6838528/1‑140 (MQ=11)
                                                                                                                                      ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt       <  2:333716/140‑1 (MQ=32)
                                                                                                                                         aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                          >  2:10117419/1‑102 (MQ=32)
                                                                                                                                         aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg    >  2:7683949/1‑140 (MQ=32)
                                                                                                                                         aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg    >  1:10816602/1‑140 (MQ=32)
                                                                                                                                           cgccgccgcCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg  <  1:7487005/140‑1 (MQ=32)
                                                                                                                                           cgccgccgcCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg  >  1:1084496/1‑140 (MQ=32)
                                                                                                                                           cgccgccgcCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg  >  2:5905450/1‑140 (MQ=32)
                                                                                                                                           |                                                                                                                                            
GATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGC‑GAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGG  >  NZ_CP025268/2233088‑2233365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑