Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2390TA34.7% 304.0 / 67.1 200intergenic (+139/+234)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base T (32/92);  new base A (66/0);  total (108/92)
Fisher's exact test for biased strand distribution p-value = 6.73e-27
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 206 total aligned reads displayed.

TGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTT  >  NZ_CP025268/2233111‑2233378
                                                                                                                                  |                                                                                                                                           
tGTCATCAGGACCGAAAAGCACGAGAATATTTCAGTCACTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCACTCGCCGCCGCCCCCTGCACCCCCggg                                                                                                                                  <  2:392347/140‑1 (MQ=25)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGATCGC‑G‑CAGGGGGCGGCCCATAGCCGCCGCCCCCTGCACCCCCGGGcact                                                                                                                              >  2:6041375/1‑140 (MQ=255)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGG‑CGGGGGGGGGCCAA‑AGCCGCCCCCCCCCGCAccccccgcccac                                                                                                                              >  1:9273920/1‑133 (MQ=37)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCCGGGGGGGGCCAA‑CGCCCCCCCCCCCCGCccccccccgcgct                                                                                                                              >  1:6887288/1‑133 (MQ=37)
    atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAAACTCCCCCCCCCCCAGCAcccccccgctaa                                                                                                                              >  1:7087134/1‑133 (MQ=37)
         gTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGGGGGCAAACGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGa                                                                                                                         >  2:5212581/1‑140 (MQ=255)
            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑GGGGGGGGGGGCCAACACCCCCCCCCCCACCACCCCCCGGCGCCGGGGAACa                                                                                                                      >  1:5397508/1‑140 (MQ=25)
            cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑GCGGGGGGGGGCCATCGCCGCCCCCCCCCGCACCCCCCGGCGCTGGGGcaca                                                                                                                      >  2:2980129/1‑140 (MQ=25)
              aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTGCATATAATCACTGGCGCAAGGAGCGC‑G‑CCGGGGGCGGCCCATAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAACaaa                                                                                                                    >  2:2551588/1‑140 (MQ=25)
                   aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CCGGGGGCGGCCCATCGCCGCCGCCCCCAGCCCCCCCGGGCTCTGGCGAACaaaaaagc                                                                                                               >  2:5415490/1‑136 (MQ=25)
                   aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAAACGCCCCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAAATagc                                                                                                               >  2:1608692/1‑140 (MQ=255)
                    aCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CCGGGGGCGGCCCATAGCCCCCGCCCCCAGCCCCCCCCCGCGCTGGCGAACAAAATcacc                                                                                                              >  2:15269/1‑140 (MQ=21)
                       agaATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCt                                                                                                           <  1:4671199/140‑1 (MQ=255)
                                  gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                                <  1:10427357/140‑1 (MQ=255)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑GCGGGGGCGGCCAATAGCCCCCCCCCCCACCACCCCCCCGCGCAGGCGCACAAAAACGCCGCAGGGCCGGGccca                                                                                               >  1:8684119/1‑139 (MQ=255)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCCATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                               >  2:9836804/1‑140 (MQ=255)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCCATAGCCGCCCCCCCCAACACCCCCCGGCGCAGGGGAAAAAAAAAACCCCAGCGCGGGGcccg                                                                                               >  2:4182126/1‑139 (MQ=18)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATAGCCGCCGCCCCCATCAACCCCCGGCTCAGGCGAAAAAAAACGCAGCAGCCCCGTGccca                                                                                               >  2:7209172/1‑139 (MQ=18)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAAAAGCCGCCGCCCCCTGCACCCCCCGGCTCAGGCGAACAAAAAAGCCCCTGCGCGGTGccca                                                                                               >  2:3501015/1‑139 (MQ=18)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCCCCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCt                                                                                               >  2:701485/1‑140 (MQ=255)
                                   tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAAAAGCCCCCGCCCCCAGCACCCCCCGGCTCAGGCGAACAAAAACGCCGCAGCGCGGTGccca                                                                                               >  2:4630999/1‑139 (MQ=18)
                                     tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CCGGGGGCGGCCAAAACCCCCCCCCCCCAGCACCCCCCGGCACAGGCGAACAAAAAAGCCGCAGCGCCGTGcccacc                                                                                             >  1:10870095/1‑137 (MQ=18)
                                       tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCAGCCAATAGCCGCCCCCCCCAGCAACCCCCGGCGGTGGCGAAACAAATAGCCGCTGCGCCGTGCCCACGGc                                                                                           >  1:7360121/1‑140 (MQ=18)
                                        cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CATGGGGCGGCCAATCGCCGCCCCCCCCTGCAACCCCGGGCTCTGGCGAACAAAATAGCCGCTGCGCGGTGCCCTCGGCt                                                                                          >  2:2815916/1‑140 (MQ=255)
                                           ccTGTTGCGCTCCTGTCATGTGCATTGCTGCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTTGCGAACAAAATCCCCGCTGCGCGGTGCCCTCGGCTTAt                                                                                       >  2:606807/1‑140 (MQ=255)
                                             tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGCGCGC‑G‑GAGGGGGCGGCCAATCGCCGCCGCCCCCTGGACCCCCCGGCTAAGGCGAACCAAAACGCCGCTGCGCGGTGCCCTCGGCGTATcc                                                                                     >  2:7929404/1‑140 (MQ=18)
                                              gTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CATGGGGCGGCCAATACCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAAAAGCCGCATCGCGGTGCCCACGGCGTAAccc                                                                                    >  2:10601076/1‑140 (MQ=18)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAAAAGCCCCCGCCCCCATCACCCCCCCGCACTTGCGAACAAAAACGCCCCTGCGCGGTGCCCACGGCTTATccca                                                                                   >  2:6447351/1‑139 (MQ=18)
                                               ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAAAAGCCCCCCCCCCCAGCACCCCCCGGCGCATGCGAACCAAAAAGCCGCTGCGCGGTGGCCCCGGCCTATacca                                                                                   >  2:4536818/1‑139 (MQ=18)
                                                 gcgcTCCTGTCATGTGCTGTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                                 <  1:10601076/140‑1 (MQ=255)
                                                    cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGTGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                                              <  1:2051628/140‑1 (MQ=255)
                                                    cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACgg                                                                              >  2:9108996/1‑140 (MQ=255)
                                                         gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCGGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGGCCTCCGCTTATCCCGTAAGGCTAcc                                                                         >  2:10400506/1‑140 (MQ=17)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CCGGGGGCGGCCCAACCCCGCCGCCCCCCGCACCCCCCGGCGCAGGCGCACAAAAACACCGCCGCGCGGTGCCCACGGCGTATCCCATACCGCTACCCGGTCg                                                                   >  2:3647287/1‑140 (MQ=14)
                                                               tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCCATCGCCGCCGCCCCCAGGACCCCCCGGCTCTGGCGACCAAAAACGCCGCGGCGCGGAGCCCACGGCATATCCCATACGGCTACCCCGTCg                                                                   >  2:6794017/1‑140 (MQ=14)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCTGCCAATATCCCCCCCCCCCGGCACCCCCCGGCGCAGGCGAACAAAAAATCCCCGGCGCGGTGCCCACCGCGTAAACCATAAAGCTACCCCGTCggg                                                                 >  2:1381243/1‑140 (MQ=9)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                 <  2:5100206/140‑1 (MQ=255)
                                                                 cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCggg                                                                 <  2:1035298/140‑1 (MQ=255)
                                                                  aTTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgc                                                                <  1:5231144/140‑1 (MQ=255)
                                                                   ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                               <  2:4471332/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                              <  1:3792904/140‑1 (MQ=255)
                                                                    tGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcgc                                                              <  2:8199471/140‑1 (MQ=255)
                                                                     gCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGcgcg                                                             <  2:6446674/140‑1 (MQ=255)
                                                                     gCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGcgcg                                                             <  2:7905296/140‑1 (MQ=255)
                                                                     gCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGcgcg                                                             <  1:4252855/140‑1 (MQ=255)
                                                                      cTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CCGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCCGCTAATGCGCAAAAAAACGCCCCGTGGCGGTGCCCACGGCGTATACCCTACGGCAAACAGGGAGGGcgcgc                                                            >  2:3200435/1‑139 (MQ=9)
                                                                      cTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGa                                                            <  2:3748933/140‑1 (MQ=255)
                                                                      cTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAAAAGCCCCGGCGCGGTGCCCACGGCGTATACCATACGGCTACCCGGTCGGGCGCGa                                                            >  2:1872600/1‑140 (MQ=14)
                                                                      cTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATAGCCGCCGCCCCCAGCACCCCCCGGCTCTTGCGAACAAAATCGCCGCTGCGCGGTGCCCTCCGCTTATACCATACGGCAAACCGGTCGGGCGCGa                                                            >  2:5906163/1‑140 (MQ=14)
                                                                        tCATAGAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAAACGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCATACGGCTACCGGGTCGGGCGCGAgg                                                          >  2:8296080/1‑140 (MQ=11)
                                                                           tataATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                       <  1:6785339/140‑1 (MQ=37)
                                                                           tataATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                       <  2:3582726/140‑1 (MQ=37)
                                                                              aaTCACTGGCGCAAGGAGCGC‑G‑CTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                                    <  1:3103725/140‑1 (MQ=21)
                                                                                tCACTGGCGCATGGAGCGC‑G‑CTGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                                  <  1:4909784/140‑1 (MQ=18)
                                                                                tCACTGGCGCATGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATcc                                                  <  1:2980129/140‑1 (MQ=18)
                                                                                tCACTGGCGCAAGGAGCGC‑G‑GAGGGGGCGGCCCAAAGCCCCCCCCCCCATCACCCCCCGGCTAAGGCGAAAAAAAAAGCCGCTGCGCCGTTCCCACCGCCTATACCAAAACGCTAACCGGTCAGGCGCCAGGTaaaaaac                                                  >  2:10274504/1‑135 (MQ=255)
                                                                                tCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCCATCGCCGCCGCCCCCAGCACCCCCCGGCTCAGGCGAACCAAAAAGCCCCCGCGCCGTGCCCACCGCGGATCCCATACGGCTACCGGGTGCGGCGCGCGGTAAccacc                                                  >  2:7894682/1‑136 (MQ=2)
                                                                                tCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATAGCCGCCGCCCCCAGCCCCCCCGGGCTCTGGCGAAAAAAATAGCCGCTGCGCGGTGCCCACGGCGTATACCTTAAGGCTACCGGGTCGGGCGCGAGGGAACATcc                                                  >  2:5744643/1‑140 (MQ=11)
                                                                                   cTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTg                                               <  1:6054732/140‑1 (MQ=32)
                                                                                   cTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTTGCGAAAAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCATACGGCTACCCGGTCGGGCGCGAGGTAACaaaccag                                               >  2:10002905/1‑134 (MQ=11)
                                                                                    tGGCGCAAGGAGCGC‑G‑CAGGGGGGGGCCAAAAGCCCCCCCCCCCCGCACCCCCCGGCTCTGGCGAACAAAAAACCCCCAGCCCGGGTCCCACCGCTTAACCCATAACGCTACCCTGTCGGGCGCGAGCTAACATcccact                                              >  2:743997/1‑137 (MQ=2)
                                                                                      gcgcAAGGAGCGC‑G‑CAGGGGGCGGCCAAAAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGCACAAAATACCCGCCGCGCGGTGCCCACGGCTTATCCCGTAACGCTACCCGGTCGGGCGCGAGGTAACAAACCAGTaa                                            >  2:8656759/1‑140 (MQ=11)
                                                                                          aaGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                                        <  2:8322568/140‑1 (MQ=32)
                                                                                             gAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                                     <  1:3302321/140‑1 (MQ=32)
                                                                                                cgc‑g‑cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  1:7989460/140‑1 (MQ=32)
                                                                                                cgc‑g‑cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  1:4524068/140‑1 (MQ=32)
                                                                                                cgc‑g‑cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  1:10013362/140‑1 (MQ=32)
                                                                                                cgc‑g‑cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                                  <  1:8458147/140‑1 (MQ=32)
                                                                                                      ctgggggCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                              <  1:5075946/138‑1 (MQ=17)
                                                                                                      cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                              <  2:9245189/140‑1 (MQ=32)
                                                                                                      cAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGc                              <  2:8760627/140‑1 (MQ=32)
                                                                                                       aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                             <  1:840395/140‑1 (MQ=32)
                                                                                                       aGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                             <  2:8046214/140‑1 (MQ=32)
                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAACCGCGCCCTCAGCCcaca                         <  2:8441151/140‑1 (MQ=17)
                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:10890754/140‑1 (MQ=32)
                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:10870095/140‑1 (MQ=32)
                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:10478946/140‑1 (MQ=32)
                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  1:4022995/140‑1 (MQ=32)
                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  2:3043685/140‑1 (MQ=32)
                                                                                                           ggcggcCAATCGCCGCCGCCCCCTGCACCCCCGGGCCCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca                         <  1:1660871/140‑1 (MQ=17)
                                                                                                               gcCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAGCCa                     >  2:10777606/1‑140 (MQ=17)
                                                                                                                ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAATCGCGCCCTCAGCCCACATCCAt                    >  2:4836437/1‑140 (MQ=17)
                                                                                                                ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACAtggat                    <  1:10274709/140‑5 (MQ=17)
                                                                                                                ccAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAt                    <  2:333716/140‑1 (MQ=32)
                                                                                                                 cAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtg                   <  1:7888457/140‑1 (MQ=32)
                                                                                                                   aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                       >  2:10117419/1‑102 (MQ=32)
                                                                                                                   aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTaa                                                       <  1:10117419/102‑1 (MQ=32)
                                                                                                                   aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  1:8044593/1‑140 (MQ=32)
                                                                                                                   aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg                 >  2:7683949/1‑140 (MQ=32)
                                                                                                                     cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg               >  1:1084374/1‑140 (MQ=32)
                                                                                                                          ccgccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc          <  2:9268809/140‑1 (MQ=32)
                                                                                                                          ccgccCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGcc          >  1:5956591/1‑140 (MQ=32)
                                                                                                                            gccCCCTGCACCCCCGGGCTCTGGCGAACAAAAGCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTACAACGCGCCCTCAGCCCACATCCATGTGGGCTGcccc        <  2:8294809/140‑1 (MQ=17)
                                                                                                                             cccccTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCg       <  1:3716205/140‑1 (MQ=32)
                                                                                                                              ccccTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCgg      <  2:5534223/140‑1 (MQ=32)
                                                                                                                               cccTGCACCCCCGGGCTCTGGCGAACAACATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGc     <  2:9691077/140‑1 (MQ=17)
                                                                                                                                  tGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt  <  2:668255/140‑1 (MQ=32)
                                                                                                                                  tGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt  >  2:682367/1‑140 (MQ=32)
                                                                                                                                  tGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt  >  2:5860698/1‑140 (MQ=32)
                                                                                                                                  tGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCtt  <  2:1495864/140‑1 (MQ=32)
                                                                                                                                  |                                                                                                                                           
TGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑G‑CAGGGGGCGGCCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGGCTGCCCCGGCCTT  >  NZ_CP025268/2233111‑2233378

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑