Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,3950AT23.3% 426.8 / 20.8 218intergenic (+30/‑166)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (87/78);  new base T (0/50);  total (87/131)
Fisher's exact test for biased strand distribution p-value = 4.25e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 235 total aligned reads displayed.

GAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTT  >  NZ_CP025268/164256‑164534
                                                                                                                                           |                                                                                                                                           
gAGCGTACCGTGAGGTGCAAAAAGAGATGACAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                             <  1:8418950/140‑1 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                             <  1:5810427/140‑1 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                             <  1:2458947/140‑1 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGACATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGgaga                                                                                                                                             <  2:8991930/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                          <  2:516456/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAggg                                                                                                                                          >  1:5144691/1‑140 (MQ=255)
    gTACGGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                         <  1:6783792/140‑1 (MQ=255)
    gTACCTTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                         <  1:1148884/140‑1 (MQ=255)
    gTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGc                                                                                                                                         <  1:8876611/140‑1 (MQ=255)
     tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                        >  1:2560937/1‑140 (MQ=255)
     tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                        >  2:7016530/1‑140 (MQ=255)
     tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGcc                                                                                                                                        <  2:2611797/140‑1 (MQ=255)
      aCCGTGCGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTCCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCg                                                                                                                                       <  1:6388203/140‑1 (MQ=255)
        cGTGAGGTGCAATAAGAGATGAATGGGCGTTATCCCAAACATGTGTGGCCGGACGACCGGGCAAATACTGCACCGACGCGACGTACGAAAAAGTATTCGTAAGGTTGGTTTTATCCCTCTCCCTGTGGGATTGGGCCggg                                                                                                                                     <  2:7518150/140‑1 (MQ=255)
          tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGt                                                                                                                                   >  1:8242753/1‑140 (MQ=255)
          tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTGTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGt                                                                                                                                   >  2:8686038/1‑140 (MQ=255)
               tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGACAATGTATTCGTGAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAggg                                                                                                                              >  1:8823448/1‑140 (MQ=255)
                 cAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGcc                                                                                                                            >  1:7710105/1‑139 (MQ=255)
                     aagtgttGAATGGGCGTTATCCCAAACATGTCTGGCCTGACGACCCGGCAAATACTGCACCGACGCGACGGACGAATAAGTATTCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAg                                                                                                                        <  2:9852293/134‑1 (MQ=255)
                         gttGAATGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGTGGGCCTCAgcgcg                                                                                                                    <  2:8861057/138‑1 (MQ=38)
                         gaTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                    >  1:4226994/1‑140 (MQ=255)
                         cctGACAGGGCGTTAGCCCACCCATGTCGGGCCGGACGCCCCGGCACATCCTGCACCGACCCGACGGACGAAAAATTATTCGTAAGGTTGGTGTTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcg                                                                                                                    <  2:10331647/138‑1 (MQ=25)
                           tGAATGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                  <  2:1315036/140‑1 (MQ=255)
                             aaaGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACg                                                                                                                >  2:2641204/1‑140 (MQ=255)
                               tgggCGTTATCCCAAACTGGTGTGGCCGGACGACCGGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCGTGTGGGTGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGtt                                                                                                              <  2:10419590/139‑1 (MQ=255)
                               cggccGTCATCCCCACCATGTCGGCCCGGCCGACCCGGCAAAGAATGCACCGAGGCGACGCACGAAAAAGTATTCGTAAGGGTGTTTTTCTCCATCTCCCGGTGGGATAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                              <  2:7923681/136‑1 (MQ=21)
                               aGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTTTTCGTAAGTTTGGTTTTCTCCCTCTCCCGGGGGTTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtt                                                                                                              <  2:7801304/140‑1 (MQ=38)
                                  gCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTCAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTtcac                                                                                                           >  2:9734787/1‑140 (MQ=255)
                                  gCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtggcc                                                                                                           >  1:3104960/1‑136 (MQ=255)
                                   cGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGAATTCGTAAGGTGTGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACggggacc                                                                                                          >  1:199553/1‑134 (MQ=37)
                                    tttttCCAAAACTTGGATGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTTTTCGTATGGTTGGTTTTCTCCCACGCCCTGTGGGTGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTtcaccc                                                                                                         <  2:5828091/136‑1 (MQ=255)
                                    gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggaccc                                                                                                         >  2:4984018/1‑140 (MQ=255)
                                     ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTTGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  2:428589/140‑1 (MQ=255)
                                     ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccca                                                                                                        >  2:4605260/1‑139 (MQ=255)
                                     ttATCCCAAACATGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGAATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  2:6552386/140‑1 (MQ=255)
                                     ttATCCAAAACTGGTCGGGCCGGACGACCGGCAAAATACGCCACCGCCGCGCGGTACTAAAAATTTTTCTTAAGGTTGTTTTTCTCCCTCTCCGTGGGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  1:3766559/140‑1 (MQ=16)
                                     taaccccAAAAATGTCGGGCCGGACGCCCCGGAAAATACTGCACCGCCGCGACGGACGAAAAAGTTTTCGTATGTTTGGTTTTCTCCCTCCCCCTGTGGGTGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTtcaccct                                                                                                        <  1:7368305/136‑1 (MQ=255)
                                        ccccAACCATGTCGGGCCGCACGACCCGGCAAATCCTCCACCGCCGCCACGCACGACAAAGTATTCGACAGGGGGGTTTACTCCCTCTCCCTGTGTGAGAGGGCCGGGGTGAGGCCATCAGCGCGCACGTtcaccctcac                                                                                                     <  2:8473193/139‑1 (MQ=255)
                                           cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgggcccgcacccc                                                                                                  >  1:656776/1‑139 (MQ=255)
                                                tgttcgggCCGGACGACCCGGCAAATACTGCACCTCCGCGACGGACTAAAAAGTTTTCGTAAGGTTGGTTTTCGCCCTCTCCCTGTGGTTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                             <  2:7864689/134‑1 (MQ=37)
                                                 tgtggggCCGGACGACCCGGCAAATCCTGCACCGCCCCGCGGGCCCAAAAAGTTTACGAATGGTTGGTTTTCTCCCCCACCCTGTGGGAGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCct                                                                                            <  1:8262109/135‑1 (MQ=255)
                                                  ggcTGGCCGCCCGCCCCGGCCAATCCTGCACCGACGCGACGGACGCAAAAGTATTCGTAAGGTTGGTTTTCCCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                           <  2:5973738/138‑1 (MQ=37)
                                                  gTCTGGCCTGACGACACGCCAAATACTCCACCGACGCGACGTACGAAAAATTATTCGTATGGTTGTTTTACTCCCTAACCCTGTGGTAGAGGGCAGGGGTGAGGGCATCATCGCGCACGTTCACCCTCACCCTAACCctc                                                                                           <  2:6818874/140‑1 (MQ=255)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCTAAcccac                                                                                           >  2:7407540/1‑138 (MQ=255)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAAcccac                                                                                           >  1:9193515/1‑138 (MQ=255)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCctc                                                                                           >  2:7212536/1‑140 (MQ=255)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCACCCAAAcacac                                                                                           >  1:7017183/1‑136 (MQ=37)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACAACCAAAaccaa                                                                                           >  2:3674970/1‑138 (MQ=37)
                                                  gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACCCCCAAAaccac                                                                                           >  1:8119688/1‑138 (MQ=37)
                                                  gTCTGGCCGGACGACCCGCCAAATACTCCACCGACGCGAGGGACTAAAATTTTTTGTTATGGTTGGTTTTCTCCCTCCCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                           <  1:1752933/140‑1 (MQ=25)
                                                  gTCGGGCGGGACGACCCGGAAAATCCTGCCCCGACGAGACGGACTAAAAATTATTCTTATGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGTCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                           <  2:3532351/140‑1 (MQ=25)
                                                    cgggccggccGCCCCGGAAAATACGGCACCGACGCGACGTCCGAAAAAGTTTGCGTATGGTTGGTTTTCTCCCTCTCCGTGTGGGAGTGGGCGGGGTTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                         <  2:3073411/138‑1 (MQ=255)
                                                    cgggccggccGACCCGGAAAATACTGCACCGACGCGACGGACGAAAACGTATTCGTAGGGTTGTTTTTCTCCCTCTCCCTGGGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                         <  2:1415793/138‑1 (MQ=37)
                                                    cTGGCCGGACGCCCCGGAAAATACTCCACCGACGCGACGTACGAAAATGTATTCGTACGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                         <  1:4776356/140‑1 (MQ=37)
                                                    cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                         <  2:7127878/140‑1 (MQ=255)
                                                    cTGGCCGGACGACCCGGCAAATACTCCACCGACGCGACGGACGAATATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCTCCCTCACCCTAACCCtctc                                                                                         <  2:2847639/140‑1 (MQ=255)
                                                       gCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCt                                                                                      <  2:3774748/140‑1 (MQ=38)
                                                       gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                      <  2:571452/140‑1 (MQ=255)
                                                       gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTGGGTTTTCTCCCGCCCCCTGTGGGAGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                      <  2:6388718/140‑1 (MQ=255)
                                                        ccGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTGCACCCACAACCAAAACCACAcccac                                                                                     >  2:1948501/1‑138 (MQ=37)
                                                          ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCGGCGCGCACGTTCACCCTCACCCTAACCCTCTcccgcca                                                                                   >  2:9054129/1‑136 (MQ=255)
                                                           gccgacCCGGCAAAAACTGCCCCGACCCGACGGACGAAAAAGTATTCGTACGGTTGGTTTACCCCCCCCCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                  <  1:7183195/138‑1 (MQ=255)
                                                           gacgacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                  <  2:6416889/140‑1 (MQ=255)
                                                              gacCCGGCAAATACTGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga                                                                               <  1:8620561/140‑1 (MQ=255)
                                                              gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga                                                                               <  1:10807102/140‑1 (MQ=255)
                                                               acccttcAAATACTGCACCGACGCGACGTAGGATATTTTATTCTTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                              <  2:9478754/134‑1 (MQ=25)
                                                               acCCGGCAAATCCGGCACCGACGCGACGGACTAAAAAGTTTTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                              <  2:9551281/140‑1 (MQ=38)
                                                               acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                              <  2:6072426/140‑1 (MQ=255)
                                                                 ccGGCAAATCCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                            <  2:4712166/140‑1 (MQ=255)
                                                                          aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCAAACCCACACCCGCCAGGGAGAGGGGCCCGAt                                                                   >  1:9909492/1‑140 (MQ=37)
                                                                          aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCAAAACCTCTCCCTCACGGGCGAGGGGTccccg                                                                   >  2:10704610/1‑137 (MQ=25)
                                                                                cggacgcgaggGACTAAAATGTTTCCTTTTGGTGGGTTTGCTCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                             <  1:8068684/138‑1 (MQ=25)
                                                                                ccgccgCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                             <  2:9336278/140‑1 (MQ=38)
                                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGCCCCACACCCCAAACCACTCCCACAAGGGGGGGGGGGCCGCTAGAGCACaa                                                          >  1:10178566/1‑140 (MQ=21)
                                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGCCCCCACACCCAAACCCAAACCCACAAGGGGGTGGGGACCGGTCGCGCACaa                                                          >  1:7284432/1‑140 (MQ=25)
                                                                                   acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCTAACCCACTCCCTCAAGGGAGAGGGGACCGATCGAGCACaa                                                          >  2:9679960/1‑140 (MQ=255)
                                                                                      cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAACCCGCTCCCACAAGGGAGAGGGGGCCGATAGAGCCCAAAtt                                                       >  2:1707528/1‑140 (MQ=37)
                                                                                        acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAAtttt                                                     <  2:10248294/140‑1 (MQ=255)
                                                                                           gacgAAAAATTATGCGTATGGTTGGTTGTCTCCCTCTCCGTGTGGGTGGGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgag                                                  <  1:9425218/140‑1 (MQ=38)
                                                                                           gacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCAACCGCACCCAAAACCACACCCCCACTGGGGTGGGGGCCCATCGAGCCCAAAATTTgtg                                                  >  1:9157749/1‑138 (MQ=18)
                                                                                             ctaaaatGTATGCGTATGGTTGTTTTTCTCCCTCGCCGTGTGGTTGCGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                <  1:10336509/138‑1 (MQ=37)
                                                                                             ctaaaaaGTTTGCTTACGTTTGGTTTTCTCCCTCACCCTGGGGGAGAGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCACGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                <  2:1994272/138‑1 (MQ=37)
                                                                                             ctaaaaaGTATTCGTATGGTTGGTTTTCACCCACTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTgagag                                                <  1:10563698/138‑1 (MQ=39)
                                                                                             cgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGAAACCACACCCGAACCCTCAACCGCATGGGAGAGGGGAACGATGGAGCACAAATTTTgagag                                                >  2:2758342/1‑140 (MQ=25)
                                                                                             cgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCCCCCTCACCCTAACCCACTACCTCAAGGGCGAGGGGACCGATCGAGCCCAAATTTTgtgag                                                >  2:9122591/1‑140 (MQ=37)
                                                                                               aaaatttATTCTTATGTTGGGTTTTCTCCCTCCCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                              <  1:1707528/134‑1 (MQ=37)
                                                                                               aaaaaTTATTAGTTTGTTTTGTTTCCTCCATCTCCCGGTGGTTGTTGGCCGGTGTGAGGGCATCAGCGCGCACGTTCACCCTCAACCTAACACTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGat                                              <  1:6520778/140‑1 (MQ=255)
                                                                                                 aaaGTATTCTTTCGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                            <  1:10785407/140‑1 (MQ=255)
                                                                                                 aaaGAATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGCGTGGGCCGGGGTTTGGTCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGatat                                            <  2:4314312/140‑1 (MQ=39)
                                                                                                     ttttCGTACGGTTGGTTGTCTCCCTCGCCCTGGGGGCGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttc                                        <  2:2371965/138‑1 (MQ=39)
                                                                                                       ttcttttggttgtttTTCGCCCTCCCCGTGGGGTGGTGGGCGGGGGGGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                      <  1:24136/133‑1 (MQ=37)
                                                                                                       ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttctt                                      <  2:959041/140‑1 (MQ=255)
                                                                                                        tCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATAtcttcttc                                     <  2:7474730/140‑1 (MQ=255)
                                                                                                        tCGTAAGGTTGGTTTTCTCCATCTCCCTGTGGGATTGGGCCGGGTTGTGGGCATCAGCGCGCCAGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAACGATCGAGCACAAATTTTGAGTTATAtcttcttc                                     <  2:9193284/140‑1 (MQ=255)
                                                                                                          gTATGGTTGGTTTTCCCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTg                                   <  1:587896/140‑1 (MQ=255)
                                                                                                             aGGTTGGTTTTCGCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCt                                <  2:6748384/140‑1 (MQ=255)
                                                                                                                     tttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                        <  2:4498514/140‑1 (MQ=255)
                                                                                                                       actccctcaccctGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                      <  1:5554768/139‑1 (MQ=255)
                                                                                                                                          gagGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCCCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt   <  1:6148174/140‑1 (MQ=255)
                                                                                                                                           agGGCCGGGGTGAGGGCATCAGCGCGCACGTGCCCCCGCACCCCAACCCACACCCACAAGGGTGTGGGGGCCCCACGAGCCCACATTTTGTTAGATAACATATTATGGAGTGTAACAGAAGCACAGAAAAAAGGGCgttt  >  2:1621827/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                           
GAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTT  >  NZ_CP025268/164256‑164534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑