Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,4440TA22.0% 343.5 / 15.3 145intergenic (+79/‑117)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (18/92);  new base A (30/1);  total (50/95)
Fisher's exact test for biased strand distribution p-value = 4.71e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 179 total aligned reads displayed.

TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCC  >  NZ_CP025268/164305‑164583
                                                                                                                                           |                                                                                                                                            
tgtggggCCGGACGACCCGGCAAATCCTGCACCGCCCCGCGGGCCCAAAAAGTTTACGAATGGTTGGTTTTCTCCCCCACCCTGTGGGAGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCct                                                                                                                                              <  1:8262109/135‑1 (MQ=255)
 gtgtGGCCGGCCCACCCGGAAAATACTGCACCGACGCGACGGCCTAAAATTTATTCGTAAGTTTGTTTTTCCCCCTCTCCCTGTGGGAGAGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  1:1783303/137‑1 (MQ=25)
 gacTGGCCGGACCACCCGGAAAATACCGCACCGACGCGACGGACGAAAAATTATTCGTAAGGTTGTTTTTCACCCTCACCCTGTGGGAGAGGGCGGGGGTGTGCGCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  1:6590561/138‑1 (MQ=255)
 gTCTGGCCTGACGACACGCCAAATACTCCACCGACGCGACGTACGAAAAATTATTCGTATGGTTGTTTTACTCCCTAACCCTGTGGTAGAGGGCAGGGGTGAGGGCATCATCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  2:6818874/140‑1 (MQ=255)
 gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAACAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCAAACCctc                                                                                                                                             >  2:2749512/1‑140 (MQ=255)
 gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTGACCCTCACCCAAACCctc                                                                                                                                             >  2:514650/1‑140 (MQ=255)
 gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCctc                                                                                                                                             >  2:7212536/1‑140 (MQ=255)
 gTCTGGCCGGACGACCCGGCAAATACGCCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  2:7725331/140‑1 (MQ=38)
 gTCGGGCGGGACGACCCGGAAAATCCTGCCCCGACGAGACGGACTAAAAATTATTCTTATGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGTCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  2:3532351/140‑1 (MQ=25)
   cTGGCCGGACGCCCCGGAAAATACTCCACCGACGCGACGTACGAAAATGTATTCGTACGGTTGTTTTTCTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                                                           <  1:4776356/140‑1 (MQ=37)
   cTGGCCGGACGACCCGGCAAATACTCCACCGACGCGACGGACGAATATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCTCCCTCACCCTAACCCtctc                                                                                                                                           <  2:2847639/140‑1 (MQ=255)
    gggCGGGACGCCCCGTAAAATACGCCACCGACGCGCCGGCCTAAAATGTATTCGTAAGGTTGGTTTACCCCCTCTCCCTGTGGGCGCGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                                                                          <  1:10750767/139‑1 (MQ=255)
      gCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCt                                                                                                                                        <  2:3774748/140‑1 (MQ=38)
      gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                                                        <  2:571452/140‑1 (MQ=255)
      gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTGGGTTTTCTCCCGCCCCCTGTGGGAGTGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCt                                                                                                                                        <  2:6388718/140‑1 (MQ=255)
       ccGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTGCACCCACAACCAAAACCACAcccac                                                                                                                                       >  2:1948501/1‑138 (MQ=37)
         ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACACCCAAAACCACTcccgcca                                                                                                                                     >  2:1406463/1‑136 (MQ=37)
             gacCCGGCAAATACTGCACCGCCGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga                                                                                                                                 <  1:8620561/140‑1 (MQ=255)
             gacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCACAGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga                                                                                                                                 <  1:10807102/140‑1 (MQ=255)
              ccccGGCAAATACTGCACCGACGCGACGGCCGAAAAAGTATTCGAAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                                <  2:8544370/139‑1 (MQ=255)
              acccttcAAATACTGCACCGACGCGACGTAGGATATTTTATTCTTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                                <  2:9478754/134‑1 (MQ=25)
              acCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                                <  2:6072426/140‑1 (MQ=255)
                ccGGCAAATCCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                              <  2:4712166/140‑1 (MQ=255)
                         aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGACCCACACCCAAACCCACAGCCGCACGGGCGAGGGGACCggt                                                                                                                     >  1:4592780/1‑138 (MQ=25)
                         aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACTCCCACAAGGGAGAGGGGACCGAt                                                                                                                     >  2:708460/1‑140 (MQ=255)
                             cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCGAACCCAAACCCACTACCGCAAGGGTGAGGGGACCGAACGAg                                                                                                                 >  2:5378980/1‑140 (MQ=25)
                               cggacgcgaggGACTAAAATGTTTCCTTTTGGTGGGTTTGCTCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑ca                                                                                                              <  1:8068684/138‑1 (MQ=25)
                               ccgccgCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑ca                                                                                                              <  2:9336278/140‑1 (MQ=38)
                                 gacgcgacgGACGAAAATGTATTCGAAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑caca                                                                                                            <  2:7913703/140‑1 (MQ=255)
                                 gacgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCAAAACCACACCCACCACGGGGAGGGGACCGCACGAG‑gacc                                                                                                            >  1:6537100/1‑139 (MQ=21)
                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGCCCCACACCCCAAACCACTCCCACAAGGGGGGGGGGGCCGCTAGAG‑CACaa                                                                                                           >  1:10178566/1‑140 (MQ=21)
                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCAAACCCACTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACaa                                                                                                           >  2:9680101/1‑140 (MQ=255)
                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCGCCCGCCACCAAACCCACGCCCCCCACGGGGGGGGGGCCCAGCGCG‑CACaa                                                                                                           >  1:9031582/1‑140 (MQ=18)
                                     ctccggccgAAAAAGTATGTTTATGGTTGTTTTTCTCCGTCACCCTGTGGGTGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAAtt                                                                                                        <  1:9164323/137‑1 (MQ=37)
                                     cgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAACCCGCTCCCACAAGGGAGAGGGGGCCGATAGAG‑CCCAAAtt                                                                                                        >  2:1707528/1‑140 (MQ=37)
                                       acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCGCAACCAAAACCACACCCCCAACGGTGAGGGGGCCGATCGAG‑GACcaaaatt                                                                                                      >  1:1470414/1‑136 (MQ=18)
                                            ctaaaaaGTATTCGTATGGTTGGTTTTCACCCACTCCCTGTGGGTGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                                 <  1:10563698/138‑1 (MQ=39)
                                            cgAAAAATTATTCGTATGGTTGGTTTTCTCCCTCGCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                                 <  2:656776/140‑1 (MQ=255)
                                            cgAAAAATTATTCGAACGGTTGTTTTACTCCCACACCCTGGGGGAGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                                 <  2:1993187/140‑1 (MQ=38)
                                            cgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGAAACCACACCCGAACCCTCAACCGCATGGGAGAGGGGAACGATGGAG‑CACAAATTTTgagag                                                                                                 >  2:2758342/1‑140 (MQ=25)
                                            cgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCCCCCTCACCCTAACCCACTACCTCAAGGGCGAGGGGACCGATCGAG‑CCCAAATTTTgtgag                                                                                                 >  2:9122591/1‑140 (MQ=37)
                                            cgAAAAAGTATTCGTAAGGTGGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                                 <  2:10905696/140‑1 (MQ=255)
                                              aaaatttATTCTTATGTTGGGTTTTCTCCCTCCCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGat                                                                                               <  1:1707528/134‑1 (MQ=37)
                                                aaaGTATTCTTTCGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGatat                                                                                             <  1:10785407/140‑1 (MQ=255)
                                                aaaGAATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGCGTGGGCCGGGGTTTGGTCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGatat                                                                                             <  2:4314312/140‑1 (MQ=39)
                                                    ttttCGTACGGTTGGTTGTCTCCCTCGCCCTGGGGGCGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttc                                                                                         <  2:2371965/138‑1 (MQ=39)
                                                      ttcttttggttgtttTTCGCCCTCCCCGTGGGGTGGTGGGCGGGGGGGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttctt                                                                                       <  1:24136/133‑1 (MQ=37)
                                                      ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttctt                                                                                       <  2:959041/140‑1 (MQ=255)
                                                       tCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                                                                      <  2:7474730/140‑1 (MQ=255)
                                                       tCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGTAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                                                                      <  1:8225026/140‑1 (MQ=255)
                                                         gTATGGTTGGTTTTCCCCCTCTCCCTGTGGGTGAGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTg                                                                                    <  1:587896/140‑1 (MQ=255)
                                                          tatgtttgtttTTCCCCCGCCCCCTGTGGGTGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGt                                                                                   <  1:5583054/135‑1 (MQ=37)
                                                            cggttggttTTCCCCCTCTCCCTGGGGGTGTGGGCGGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCt                                                                                 <  1:5620681/139‑1 (MQ=39)
                                                            aGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCt                                                                                 <  1:6443805/140‑1 (MQ=255)
                                                               ttggttTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTTCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                                                              <  2:6143905/140‑1 (MQ=255)
                                                                    tttCACCCTCTCCCTGTGGGAGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                                                                         <  1:2458560/140‑1 (MQ=255)
                                                                      acacccgctccctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                                                       <  1:7197919/137‑1 (MQ=255)
                                                                            tctccctGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAg                                                                 <  1:7320654/140‑1 (MQ=255)
                                                                                         gagGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCCCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt                                                    <  1:6148174/140‑1 (MQ=255)
                                                                                         gagGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt                                                    <  2:361767/140‑1 (MQ=255)
                                                                                          agGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAACCTCTCCCTAAAGGGGGATGGGAGCGATCGGGCCCCAAATTTTTTG‑GGTATATTATTATGTGTTGTTAAAGTCGAAAATAAAATAGGGCtttt                                                   >  2:9546693/1‑140 (MQ=255)
                                                                                           gggCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                                                  <  1:9272051/140‑1 (MQ=255)
                                                                                           gggCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                                                  <  1:7370805/140‑1 (MQ=255)
                                                                                           gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                                                  <  2:3734469/140‑1 (MQ=255)
                                                                                           gggCCGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                                                  <  1:3271623/140‑1 (MQ=255)
                                                                                            ggCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACACCCAAAACCACAACCACAAGGGAGAGTGGACCGAACGAT‑CACAAAATTTGAGCTATAAATTCTTGTTTCTTGTAAAAAAAGAACAGAAAAAAGGGcgatttg                                                 >  2:7664023/1‑134 (MQ=255)
                                                                                            ggCCGGGGTGAGGGCATCAGCGCGCACGTTCTCCCACACCCAAAACCAATACCAAAATGGTTAAGGGACCGCTCGTG‑CACACAATTTGAGAGATAAATTAGTTTTTCTTGTTACACAAAAACAGTAAAAAGGGGtttttc                                                 >  2:905136/1‑138 (MQ=255)
                                                                                              ccGGGGTGAGGGCCTCCGCGCGCACGTGCACCCACACCCAAACCCTCTCCCGCAAGGGCGCGGGGACCGATCGAG‑CCCAAATTTTGAGAGATATATTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                               >  2:10656122/1‑140 (MQ=255)
                                                                                              ccGGGGTGAGGGCCTCAGCGCGCACGTTCACCCACACCCAAAACCACACCCACAAGGGTGATGGGACCCCGCGGG‑CCAACATTTTTAGAGATATATTATTAGGTCGTGTAACAGAAAAACAGAAAATCGGGCattttagc                                               >  2:3864314/1‑136 (MQ=255)
                                                                                              ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                               <  1:10102546/140‑1 (MQ=255)
                                                                                              ccGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCACAACCACAACCAAAAGGGAGTGGGGAACCGTCGAG‑CCAAAATTGTGATAGATATATTATTATGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTtcgc                                               >  2:8090241/1‑140 (MQ=255)
                                                                                                ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt                                             <  2:9193515/140‑1 (MQ=255)
                                                                                                          cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg                                   <  1:4674926/140‑1 (MQ=255)
                                                                                                              aGCGCGCACGTTCCCCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGgaga                               <  2:1609894/140‑1 (MQ=255)
                                                                                                              aGCGCGCACGTGCACCCTCACCCTAACCCGCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATGTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAACTCGGGCTTGTGCGCCTGAATATTGCGgaga                               <  2:10401410/140‑1 (MQ=255)
                                                                                                                          caccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGcc                   <  2:9909492/140‑1 (MQ=255)
                                                                                                                          caccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGcc                   <  1:1114122/140‑1 (MQ=255)
                                                                                                                               tcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGaa              <  1:6112675/140‑1 (MQ=255)
                                                                                                                               tcaccctAACCCTATCCCTCAAGGGAGAGGGGACCTATCGAG‑CACAAATTTTGAGAGAGATCTTCTTCGGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGCGAAAAAGCATGGCCGGGaa              <  2:199553/140‑1 (MQ=255)
                                                                                                                                     tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcg        <  2:4929416/140‑1 (MQ=255)
                                                                                                                                     tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcg        <  1:1746083/140‑1 (MQ=255)
                                                                                                                                     tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcg        <  2:6194685/140‑1 (MQ=255)
                                                                                                                                     aaaCCCACACCCACAAGGGAGAGGGGACCGCTCGAG‑CACAAAATTTGAGATATAACTTCTTCTGTCTTGTAACAGAAGAACAGAAAAACGGGCTTTTTCGCCGGAATAATGCGGAGAAAAATCAATGCCGGGAAAGAccc        >  2:1241004/2‑139 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCATGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGACACTCGGGCTTTTGCGCCTGAAGATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg      >  2:6587570/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                               <  1:4242916/99‑1 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGACTGACcgcg      >  1:10287311/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg      >  1:7815682/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg      >  1:2225830/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCGGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg      >  1:9623931/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCGTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGACTGACcgcg      >  1:5422391/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGCAATAGCATGGCCGGGAATGACCGCGAGcc  >  1:8962596/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  2:5856309/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:1651194/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:3054521/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  2:10244896/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:6488568/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:8128217/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:8500156/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:9522396/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:9522468/1‑140 (MQ=255)
                                                                                                                                           |                                                                                                                                            
TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCC  >  NZ_CP025268/164305‑164583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑