BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	550,382	A→G	298 (TAA→TAG)	ybcF →	carbamate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	550,382	0	A	G	99.7%	2393.6 / NA	685	298 (TAA→TAG)	ybcF	carbamate kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (297/386); minor base T (1/1); total (298/387)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 685 total aligned reads displayed.

CCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTA  >  NZ_CP025268/550246‑550518

 cGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGt                                                                                                                                      <  1:8081562/140‑1 (MQ=255)

    tGGTTCGCTGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAgg                                                                                                                                   <  1:1192525/140‑1 (MQ=255)

    tGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAgg                                                                                                                                   <  1:492561/140‑1 (MQ=255)

      gTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCa                                                                                                                                 <  1:6892013/140‑1 (MQ=255)

      cttCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCTGAAGCATTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCAGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCa                                                                                                                                 <  2:1512570/139‑1 (MQ=255)

         cGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGGGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTa                                                                                                                              >  1:6163152/1‑140 (MQ=255)

          gATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAg                                                                                                                             >  1:5333606/1‑140 (MQ=255)

           aTGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGa                                                                                                                            <  1:3052894/140‑1 (MQ=255)

           aTGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGa                                                                                                                            <  2:3267375/140‑1 (MQ=255)

               ggCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACAtt                                                                                                                        >  2:7141548/1‑140 (MQ=255)

                gCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACAttt                                                                                                                       >  1:6367596/1‑140 (MQ=255)

                gCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACAttt                                                                                                                       >  2:10326234/1‑140 (MQ=255)

                  cGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTtgt                                                                                                                     <  1:6718348/140‑1 (MQ=255)

                    aaTGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGc                                                                                                                   >  2:9304770/1‑140 (MQ=255)

                      tGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCt                                                                                                                 >  1:10452662/1‑140 (MQ=255)

                       gTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTg                                                                                                                <  2:4334946/140‑1 (MQ=255)

                         aaCGGCGGTGCGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGAGTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGACGCGGGGCCCTGTATTTCGCTGTAGTCGTAGGCAGTAGACATTTGTGCCTGAt                                                                                                              >  2:8680045/1‑140 (MQ=255)

                         aaCGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGAt                                                                                                              >  1:7550798/1‑140 (MQ=255)

                          aCGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATg                                                                                                             >  2:10769372/1‑140 (MQ=255)

                           cggcggTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGc                                                                                                            <  1:8519175/140‑1 (MQ=255)

                                gTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTAGTTCGCTGTAGTCGTAGGCATTAGACCTTTGTGCCTGATGCGACGc                                                                                                       >  2:6791391/1‑140 (MQ=255)

                                   aGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTg                                                                                                    >  1:4055912/1‑140 (MQ=255)

                                   aGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTg                                                                                                    >  1:9106716/1‑140 (MQ=255)

                                    gTGGTTATGTCAGAAGCCGTGGTAAACCCGCTTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                   <  2:5161145/140‑1 (MQ=255)

                                    gTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGa                                                                                                   <  1:2676126/140‑1 (MQ=255)

                                        ttATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGACGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGAcgcg                                                                                               <  1:1785007/140‑1 (MQ=255)

                                        ttATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGAcgcg                                                                                               <  2:465988/140‑1 (MQ=255)

                                             tCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGACTTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTa                                                                                          >  1:7789942/1‑140 (MQ=255)

                                             tCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTa                                                                                          >  1:9333410/1‑140 (MQ=255)

                                             tCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTa                                                                                          >  1:3301328/1‑140 (MQ=255)

                                              cAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                         <  2:9369616/140‑1 (MQ=255)

                                              cAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                         <  1:7322908/140‑1 (MQ=255)

                                              cAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTAt                                                                                         <  1:5461320/140‑1 (MQ=255)

                                               aGACGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACAATTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  2:3708684/140‑1 (MQ=255)

                                               aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  2:8002096/140‑1 (MQ=255)

                                               aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  1:5770073/140‑1 (MQ=255)

                                               aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  2:8444051/140‑1 (MQ=255)

                                               aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  2:10507961/140‑1 (MQ=255)

                                               aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  1:3888026/140‑1 (MQ=255)

                                               aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        >  1:3481723/1‑140 (MQ=255)

                                               aGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGGGCCTGATGCGACGCTTGACGCGTCTTATc                                                                                        <  2:2329283/140‑1 (MQ=255)

                                                 aaGCCGTGGTACACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGCCCTGTATTTCGCTGTAGTCGTAGGCAGTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                      >  2:6300860/1‑140 (MQ=255)

                                                 aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                      >  2:980832/1‑140 (MQ=255)

                                                 aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                      >  1:8007312/1‑140 (MQ=255)

                                                 aaGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAg                                                                                      >  1:4267580/1‑140 (MQ=255)

                                                      gTGGTAAACCCGCGTGGATTGGGGCGGTATCGAGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGAAATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTa                                                                                 >  2:1927188/1‑140 (MQ=255)

                                                       tGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc                                                                                >  2:773475/1‑140 (MQ=255)

                                                       tGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc                                                                                >  2:3991288/1‑140 (MQ=255)

                                                       tGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTAc                                                                                >  2:2526497/1‑140 (MQ=255)

                                                              cccGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGt                                                                         >  1:9647107/1‑140 (MQ=255)

                                                                cgcgTGGAGTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGc                                                                       <  2:8913400/140‑1 (MQ=255)

                                                                  cgTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCg                                                                     >  1:1380040/1‑140 (MQ=255)

                                                                     ggATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCAt                                                                  <  1:8838342/140‑1 (MQ=255)

                                                                          ggggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGc                                                             <  1:2877732/140‑1 (MQ=255)

                                                                           gggCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCa                                                                                       >  2:5697966/1‑113 (MQ=255)

                                                                                ttATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTgg                                                       >  1:7379316/1‑140 (MQ=255)

                                                                                ttATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTgg                                                       >  2:1713033/1‑140 (MQ=255)

                                                                                 tATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGt                                                      >  1:3675675/1‑140 (MQ=255)

                                                                                 tATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGt                                                      >  2:9063534/1‑140 (MQ=255)

                                                                                  aTCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTg                                                     >  1:4237875/1‑140 (MQ=255)

                                                                                     gcgACTTGACGAGACGCTGGCGCGCGTAGCGGTGAGCTGTTTTTCGCTGTAGTCTTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGtac                                                  <  2:10203707/140‑1 (MQ=37)

                                                                                     gcgAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGcac                                                  <  2:7881104/140‑1 (MQ=255)

                                                                                        aaTTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAAt                                               <  1:4441199/140‑1 (MQ=255)

                                                                                           tGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGcc                                            <  2:3006804/140‑1 (MQ=255)

                                                                                                agaCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatg                                       <  2:4427982/140‑1 (MQ=255)

                                                                                                 gaCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgc                                      >  1:1977323/1‑140 (MQ=255)

                                                                                                  aCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcg                                     <  1:2434181/140‑1 (MQ=255)

                                                                                                  aCGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTgatgcg                                     <  1:10614681/140‑1 (MQ=255)

                                                                                                       ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCt                                <  1:4732971/140‑1 (MQ=255)

                                                                                                       ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCt                                >  2:3427223/1‑140 (MQ=255)

                                                                                                       ggcgggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCt                                >  1:1541751/1‑140 (MQ=255)

                                                                                                          gggcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGa                             <  2:1599403/140‑1 (MQ=255)

                                                                                                            gcgAAGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACg                           <  1:10854720/140‑1 (MQ=255)

                                                                                                               aaGCGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCAt                        <  2:2213059/140‑1 (MQ=255)

                                                                                                                  cGGGGACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCtt                     >  2:4230840/1‑140 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTGCAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                   <  2:959875/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                   <  2:6064690/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                   <  1:655942/140‑1 (MQ=255)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGACTACAACCGGTGCCGCATCAGGATTTTGGTGCACAATGCCTTATGCGATGCTTGACGCATCTTAt                   <  2:1094662/140‑1 (MQ=38)

                                                                                                                    gggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTAGTGCCTGCTGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                   <  2:2936188/140‑1 (MQ=255)

                                                                                                                    gggACCTGTAGTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTAt                   <  2:8800004/140‑1 (MQ=255)

                                                                                                                     ggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATc                  >  2:10037339/1‑140 (MQ=255)

                                                                                                                     ggACCTGTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATc                  >  2:3822512/1‑140 (MQ=255)

                                                                                                                           gTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCt            <  1:10214425/140‑1 (MQ=255)

                                                                                                                           gTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCt            <  2:6879285/140‑1 (MQ=255)

                                                                                                                           gTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCt            <  1:4602934/140‑1 (MQ=255)

                                                                                                                           gTATTTCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCt            <  1:9503710/140‑1 (MQ=255)

                                                                                                                            tATTTCGCTGTAGTCGTAGGCATTGGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCAGCCGGCGATTGGTGCACTCTGCCTGATGCGCTGCTTGACGCTTCGTATGAgggcga           >  2:5895439/1‑136 (MQ=37)

                                                                                                                               ttCGCTGTAGTCGTAGGCCTGAGACATTTGTGCCTGATGCGACGCGTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACaa        >  2:135875/1‑140 (MQ=255)

                                                                                                                               ttCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACaa        <  2:4825259/140‑1 (MQ=255)

                                                                                                                                tCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  1:1670921/140‑1 (MQ=255)

                                                                                                                                tCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  1:5101720/140‑1 (MQ=255)

                                                                                                                                tCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  1:9846250/140‑1 (MQ=255)

                                                                                                                                tCGCTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  1:8290394/140‑1 (MQ=255)

                                                                                                                                tCGCTGTAGTCGTAGGCATTAGACATTTGGGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAAt       <  1:10060380/140‑1 (MQ=255)

                                                                                                                                   cTGTAGTCGTAGGCATTAGTCCTTTGTGCCTGATGCGACGCTTGACGCGTCTTATCCGGCCTACAACCGGTGCCGCATCCGGCGATTGGTGCACAATGCCTGATGCGAGGCTTGACGCATCTTCTCAGGCCTACAATggg    >  2:7408453/1‑140 (MQ=37)

                                                                                                                                   cTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGt                                                                                              >  2:5353153/1‑50 (MQ=255)

                                                                                                                                   cTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATggg    >  1:2241845/1‑140 (MQ=255)

                                                                                                                                   cTGTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCGTCTCAGGCCTACAATggg    >  2:6640533/1‑140 (MQ=255)

                                                                                                                                     gTAGTCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTa  <  2:4527395/140‑1 (MQ=255)

CCGATGGTTCGATGGGGCCGAATGTAACGGCGGTGAGTGGTTATGTCAGAAGCCGTGGTAAACCCGCGTGGATTGGGGCGTTATCGCGAATTGAAGAGACGCTGGCGGGCGAAGCGGGGACCTGTATTTCGCTGTAATCGTAGGCATTAGACATTTGTGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACAACCGGTGCCGCATCCGGCAATTGGTGCACAATGCCTGATGCGATGCTTGACGCATCTTATCAGGCCTACAATGGGTA  >  NZ_CP025268/550246‑550518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`