Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 912,850 A→G *277* (TAA→TAG) 
R337R (CGT→CGC) 		 amiD →
ybjS ←		 N‑acetylmuramoyl‑L‑alanine amidase AmiD
NAD(P)‑dependent oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268912,8500AG99.6% 2451.1 / NA 708*277* (TAA→TAG) 
R337R (CGT→CGC) 		amiD
ybjS		N‑acetylmuramoyl‑L‑alanine amidase AmiD
NAD(P)‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (406/298);  minor base T (2/1);  total (409/299)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 728 total aligned reads displayed.

GATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGA  >  NZ_CP025268/912713‑912986
                                                                                                                                         |                                                                                                                                        
gATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGcg                                                                                                                                        >  1:8645845/1‑140 (MQ=255)
     gACACCGCGCGAGCAGCGGCGCGTGATTAGGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCa                                                                                                                                   <  1:1989977/140‑1 (MQ=255)
      acacCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCTACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGACATACGGGCAGGATTAGCGCGGCAg                                                                                                                                  >  1:2366861/1‑140 (MQ=255)
       cacCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGt                                                                                                                                 >  2:8299471/1‑140 (MQ=255)
        acCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGtt                                                                                                                                >  1:7577344/1‑140 (MQ=255)
        acCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGtt                                                                                                                                >  2:396990/1‑140 (MQ=255)
        acCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGtt                                                                                                                                <  1:2256716/140‑1 (MQ=255)
          cgcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGtttt                                                                                                                              <  1:317309/140‑1 (MQ=255)
           gcgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGTCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGATATACGGGCAGGATTAGCGCGGCAGTTTTc                                                                                                                             >  1:7369028/1‑140 (MQ=255)
            cgcgAGCCGCGGCGCGTGCTTATGGCATTCCAGATGCAGTTCCGCCCGACGGGAGATAACGGCGAAGCGGATGCAGACCCGCTGGCGAGTGCCGTAGCATTGCTGGTGAAATACGGGCAGGAGTAGCGCGGCAGGTGTcc                                                                                                                            >  2:8049387/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGCTTGCCGAAGCATTGCTGGAGAATTACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  2:8753183/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  1:4313999/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  2:9636305/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  1:7683767/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  2:8558242/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  2:2644994/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  2:5817889/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  2:7766661/1‑140 (MQ=255)
            cgcgAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTcc                                                                                                                            >  2:7431942/1‑140 (MQ=255)
                     cggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTcgcg                                                                                                                   >  1:9852513/1‑140 (MQ=255)
                      ggcgCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGt                                                                                                                  >  2:28858/1‑140 (MQ=255)
                       gcgcGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTa                                                                                                                 >  1:6569005/1‑140 (MQ=255)
                                tATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAggcgg                                                                                                        <  1:2918891/140‑1 (MQ=255)
                                       ttCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtttt                                                                                                 <  2:1785008/140‑1 (MQ=255)
                                       ttCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGtttt                                                                                                 >  1:6844670/1‑140 (MQ=255)
                                        tCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAATTACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTATtc                                                                                                >  2:6887402/1‑140 (MQ=255)
                                        tCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                                                                                >  1:1869674/1‑140 (MQ=255)
                                        tCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtc                                                                                                >  1:10818850/1‑140 (MQ=255)
                                         ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtct                                                                                               >  1:9029025/1‑140 (MQ=255)
                                         ccAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTtct                                                                                               >  2:5488897/1‑140 (MQ=255)
                                            gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGATATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  2:10478610/140‑1 (MQ=255)
                                            gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  1:6207939/140‑1 (MQ=255)
                                            gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  2:10417837/140‑1 (MQ=255)
                                            gATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  2:5329064/140‑1 (MQ=255)
                                            gATGCATGTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGa                                                                                            <  2:4267813/140‑1 (MQ=255)
                                            caTGCATATCCCCCCGACGTTCAATAACGGCGAAGCGGATGCAGACACTCACGCGATTGCCGACGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCAGTGCTCGCGTCGCCAGGCGGCAGTTTACTCGa                                                                                            <  2:3420384/139‑1 (MQ=255)
                                               gCATTTCCGCCCGTCGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGAGTCGCGCGGCAGTTTTCCGTGGTCGCGTAGCCCGGCGGCAGTTTTCTCGATAc                                                                                         >  2:2118282/1‑140 (MQ=255)
                                               gCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGGGGCAGTTGTCCGTGGTCGCGTAGCCAGGCGGCCGTTGTCTCGATAc                                                                                         >  2:10731693/1‑140 (MQ=255)
                                                 atgtCCGCCCGACGTGAAATCACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCCTAGCGGGAGACATCCGGGCAGGATTAGCGCGGCAGATTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTGCTCGATACCt                                                                                       <  2:5616083/137‑1 (MQ=255)
                                                   ttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc                                                                                     <  1:1685710/140‑1 (MQ=255)
                                                   ttCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTc                                                                                     <  1:7129993/140‑1 (MQ=255)
                                                    tCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCa                                                                                    >  1:5364750/1‑140 (MQ=255)
                                                    tCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCa                                                                                    >  1:4997601/1‑140 (MQ=255)
                                                      cGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATc                                                                                  >  1:6960158/1‑140 (MQ=255)
                                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                                 <  1:10664355/140‑1 (MQ=255)
                                                       gCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATcc                                                                                 <  1:5244297/140‑1 (MQ=255)
                                                        cccGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCa                                                                                >  2:1063909/1‑140 (MQ=255)
                                                        cccGACGTTATATAACGGCGAAGCGGATGAAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCa                                                                                <  2:897488/140‑1 (MQ=255)
                                                           gACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAggg                                                                             >  1:2730269/1‑140 (MQ=255)
                                                                  tataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAc                                                                      <  2:1242012/140‑1 (MQ=255)
                                                                   ataACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGAcc                                                                     <  1:1043251/140‑1 (MQ=255)
                                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt                                                                 >  2:4715729/1‑140 (MQ=255)
                                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt                                                                 >  2:9134860/1‑140 (MQ=255)
                                                                       cGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCt                                                                 >  2:5040737/1‑140 (MQ=255)
                                                                        ggCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTg                                                                >  1:5831242/1‑140 (MQ=255)
                                                                         gCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGa                                                               <  1:3968539/140‑1 (MQ=255)
                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATAcc                                                                                        >  2:3183782/1‑114 (MQ=255)
                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                              >  1:4183989/1‑140 (MQ=255)
                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                              >  2:4976459/1‑140 (MQ=255)
                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                              >  2:6139060/1‑140 (MQ=255)
                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                              >  1:9571094/1‑140 (MQ=255)
                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                              >  1:4731864/1‑140 (MQ=255)
                                                                          cGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGAt                                                              >  1:7931552/1‑140 (MQ=255)
                                                                            aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATaa                                                            >  2:2987293/1‑140 (MQ=255)
                                                                            aaGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATaa                                                            >  2:3701509/1‑140 (MQ=255)
                                                                            aaGAGGATTCACAAACTCAGGCTATAGCCTAAGCAAAGCAGGAGAAATACGGGCAGGATTATCGCGGCAGTTTTCAGAGGACTCGAAGCCAGACGGCAGTTTTCTCGATACATTCATAAAGGGTGATGAAAGCCTGATaa                                                            >  2:9376324/1‑140 (MQ=255)
                                                                             aGCGGATGCAGAAACTCAGGCGATTGCCGATGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAAc                                                           <  1:8893480/140‑1 (MQ=255)
                                                                             aGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAAc                                                           <  2:8143639/140‑1 (MQ=255)
                                                                               cGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCt                                                         <  1:10301855/140‑1 (MQ=255)
                                                                                 gATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTaa                                                       >  1:1190860/1‑140 (MQ=255)
                                                                                   tGCAGACACTCAGGCGAGTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAAct                                                     <  2:7566804/140‑1 (MQ=255)
                                                                                   tGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAAct                                                     >  1:3374043/1‑140 (MQ=255)
                                                                                            tCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTgcg                                            <  2:1318990/140‑1 (MQ=255)
                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  2:1671585/140‑1 (MQ=255)
                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  1:3732789/140‑1 (MQ=255)
                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  2:4576128/140‑1 (MQ=255)
                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  2:1963534/140‑1 (MQ=255)
                                                                                               ggCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGAAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  1:5679960/140‑1 (MQ=255)
                                                                                               ggCGATTGCCGAAGCAGTGCTGGAGACAGACGGGCAGGATTAGCGCGGCAGTGTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAc                                         <  2:7068975/140‑1 (MQ=255)
                                                                                                gCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAcg                                        >  1:9136145/1‑140 (MQ=255)
                                                                                                 cGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCAcgc                                       <  1:7487310/140‑1 (MQ=255)
                                                                                                    ttGCCGAAGCATTGCTGGAGAAATACTGGCAGGATTAGCGCGGCAGTTTTCCGTTGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                    <  2:2256702/140‑1 (MQ=255)
                                                                                                    ttGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                    <  1:3573399/140‑1 (MQ=255)
                                                                                                    ttGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                    >  1:6400904/1‑140 (MQ=255)
                                                                                                    ttGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTa                                    >  2:596038/1‑140 (MQ=255)
                                                                                                     tGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTaa                                   >  1:1433733/1‑140 (MQ=255)
                                                                                                        cGAAGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAatat                                >  1:851375/1‑140 (MQ=255)
                                                                                                          aaGCATTGCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATcc                              >  1:10087514/1‑140 (MQ=255)
                                                                                                                 gCTGGAGAAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTaa                       <  1:8314405/140‑1 (MQ=255)
                                                                                                                      agaAAGACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCa                  <  2:9800800/140‑1 (MQ=255)
                                                                                                                       gaAATACGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCaa                 >  1:9344786/1‑140 (MQ=255)
                                                                                                                            aCGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTc            <  1:5478293/140‑1 (MQ=255)
                                                                                                                             cGGGCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCa           >  2:6748219/1‑140 (MQ=255)
                                                                                                                               ggCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc         >  2:6331285/1‑140 (MQ=255)
                                                                                                                               ggCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc         >  2:1518107/1‑140 (MQ=255)
                                                                                                                               ggCAGGATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAAc         >  1:9648451/1‑140 (MQ=255)
                                                                                                                                    gATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGga    >  2:4111539/1‑140 (MQ=255)
                                                                                                                                    gATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGga    <  2:2402333/140‑1 (MQ=255)
                                                                                                                                    gATTAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGga    >  1:10001883/1‑140 (MQ=255)
                                                                                                                                      ttAGCGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGgaga  <  2:1138316/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GATATGACACCGCGCGAGCAGCGGCGCGTGATTATGGCATTCCAGATGCATTTCCGCCCGACGTTATATAACGGCGAAGCGGATGCAGAAACTCAGGCGATTGCCGAAGCATTGCTGGAGAAATACGGGCAGGATTAACGCGGCAGTTTTCCGTGGTCGCGTAGCCAGGCGGCAGTTTTCTCGATACCTTCATCCAGGGTGATGACCGGCTGATAACCTAACTCTTCCTGCGCACGCGTAATATCCAGCGTAAAGTCAAAATTCAACTTGGAGA  >  NZ_CP025268/912713‑912986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑