BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,236,662	T→C	A277A (GCA→GCG)	hlyE ←	hemolysin HlyE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,236,662	0	T	C	99.8%	5660.5 / NA	1617	A277A (GCA→GCG)	hlyE	hemolysin HlyE
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (950/664); minor base A (2/1); total (952/665)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 1621 total aligned reads displayed.

GTTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCTGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCG  >  NZ_CP025268/1236526‑1236798

  ttttAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGcttc                                                                                                                                     <  2:130047/140‑1 (MQ=255)

   tttAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGcttct                                                                                                                                    >  2:1118517/1‑140 (MQ=255)

    ttAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGcttctt                                                                                                                                   >  1:6056077/1‑140 (MQ=255)

     tAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCttt                                                                                                                                  >  2:25429/1‑140 (MQ=255)

           tgATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCaa                                                                                                                            >  1:3936239/1‑140 (MQ=255)

               aaaCCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCaaagaa                                                                                                                        <  2:8242252/140‑1 (MQ=255)

               aaaCCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCaaagaa                                                                                                                        <  1:271503/140‑1 (MQ=255)

                     tataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCAtt                                                                                                                  >  1:1577628/1‑140 (MQ=255)

                      ataCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTa                                                                                                                 >  1:6454047/1‑140 (MQ=255)

                        aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:7864382/140‑1 (MQ=255)

                        aCCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTaaa                                                                                                               <  1:2969002/140‑1 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGANTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGAAAAGAAAGCATTAAatcat                                                                                                           >  1:10133938/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  1:116829/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  2:10768750/1‑140 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcag                                                                                                           >  2:9717907/1‑139 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcag                                                                                                           >  1:10590699/1‑139 (MQ=255)

                            tGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACATCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTGTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcat                                                                                                           >  2:8762692/1‑140 (MQ=255)

                             gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  1:769192/1‑140 (MQ=255)

                             gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  2:3077039/1‑140 (MQ=255)

                             gAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          >  2:5070129/1‑140 (MQ=255)

                                tACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATa                                                                                                       >  1:9880682/1‑140 (MQ=255)

                                  cATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     <  1:2304879/140‑1 (MQ=255)

                                  cATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     <  1:1411330/140‑1 (MQ=255)

                                   aTGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATc                                                                                                    >  1:10848615/1‑140 (MQ=255)

                                   aTGGAGAGAATAATAGCTTATCAGACTTCAGGTACCTAAAAGTGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTTCAGGTGTTAATCA‑TTTTTTTGTCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATATTc                                                                                                    <  2:6565797/140‑1 (MQ=255)

                                    tGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCa                                                                                                   >  2:5298723/1‑140 (MQ=255)

                                     ggAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCaa                                                                                                  >  2:4542138/1‑140 (MQ=255)

                                     ggAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCaa                                                                                                  >  1:10306489/1‑140 (MQ=255)

                                     ggAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCaa                                                                                                  >  1:6912257/1‑140 (MQ=255)

                                       agagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                <  1:5836637/140‑1 (MQ=255)

                                       agagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                >  2:8965696/1‑140 (MQ=255)

                                       agagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                <  1:32494/140‑1 (MQ=255)

                                       agagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACg                                                                                                <  1:7331781/140‑1 (MQ=255)

                                        gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGTGTGTATTTTTACAGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGTAAGCATTAAATCATCATAATCAACGt                                                                                               >  2:2720264/1‑140 (MQ=255)

                                        gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  1:7177926/1‑140 (MQ=255)

                                        gagaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGt                                                                                               >  1:2509722/1‑140 (MQ=255)

                                         agaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTa                                                                                              <  2:2906674/140‑1 (MQ=255)

                                         agaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTa                                                                                              <  1:9228292/140‑1 (MQ=255)

                                          gaATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCATTTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTa                                                                                              >  1:10002999/1‑140 (MQ=255)

                                                tCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTg                                                                                       >  2:5480332/1‑140 (MQ=255)

                                                   cTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgtt                                                                                    >  1:10681824/1‑140 (MQ=255)

                                                     tATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAatcatc                                                                                                          <  2:5656449/116‑1 (MQ=255)

                                                     tATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGgttgt                                                                                  >  2:4347948/1‑140 (MQ=255)

                                                           aCTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGt                                                                            >  2:9314339/1‑140 (MQ=255)

                                                             ttCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGttt                                                                          <  1:8044401/140‑1 (MQ=255)

                                                             ttCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGttt                                                                          <  1:8145260/140‑1 (MQ=255)

                                                             ttCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGttt                                                                          <  1:67450/140‑1 (MQ=255)

                                                              tCAGGTACCTCAAAGAGTGTGTTTTTACCGTGTCTTTTCTGATACTCAGTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATACTCAACGTAGAATCTGGTTGTTTCAGtttg                                                                         >  2:846366/1‑139 (MQ=255)

                                                              tCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTc                                                                         >  1:3512633/1‑140 (MQ=255)

                                                              tCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTc                                                                         >  1:5385225/1‑140 (MQ=255)

                                                               cAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTcc                                                                        <  1:3684543/140‑1 (MQ=255)

                                                                aGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAGAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCg                                                                       >  1:9449664/1‑140 (MQ=255)

                                                                 ggTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGt                                                                      <  2:10025178/140‑1 (MQ=255)

                                                                  gTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGtt                                                                     >  2:3690021/1‑140 (MQ=255)

                                                                       tCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTAt                                                                >  1:2132582/1‑140 (MQ=255)

                                                                             agTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCAcc                                                          >  1:130586/1‑140 (MQ=255)

                                                                              gtgtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCg                                                         >  2:1958631/1‑140 (MQ=255)

                                                                               tgtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTAt                                                                >  1:4498112/1‑132 (MQ=255)

                                                                                gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGTATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       >  2:6878280/1‑140 (MQ=255)

                                                                                gtCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGAt                                                       <  2:4085225/140‑1 (MQ=255)

                                                                                gtCATTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCAACGAt                                                       >  2:5939989/1‑140 (MQ=255)

                                                                                 tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATg                                                      <  1:8317185/140‑1 (MQ=255)

                                                                                 tCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATg                                                      >  2:8081983/1‑140 (MQ=255)

                                                                                 tCTTTTTACCGTGTATATTCTTATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCATCTTTTAGCAAAGAAAGCATTAAATCATCAAAATCAAAGTAGAATCTGTTTGTTTCTGTTTCCGTTTTTATCTCACAGATg                                                      >  2:7608865/1‑140 (MQ=255)

                                                                                   tttttACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATggc                                                    <  2:2395399/140‑1 (MQ=255)

                                                                                   tttttACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATggc                                                    <  2:1518921/140‑1 (MQ=255)

                                                                                   tttttACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATggc                                                    <  1:8893105/140‑1 (MQ=255)

                                                                                     tttACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATggcgg                                                  >  2:2153488/1‑140 (MQ=255)

                                                                                      ttACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATggcggc                                                 >  1:4701830/1‑140 (MQ=255)

                                                                                      gtcccGGGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATggcggc                                                 <  2:6335831/137‑1 (MQ=255)

                                                                                           gtgtCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTAttt                                            >  1:3153121/1‑140 (MQ=255)

                                                                                           gtgtCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTAttt                                            >  1:4286266/1‑140 (MQ=255)

                                                                                           gtgtCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTAttt                                            >  1:9676210/1‑140 (MQ=255)

                                                                                            tgtCTTTTCTGATTCTCATTACAGGTGTTAATCA‑TTTTTTTGGGCGCTTCTTTTAGCACTGAAAGCATTATATCATCCTATTCAACGTAGTATCTGGTTGTTTCAGTTTCCGAGTTTTTCTCACCGTTGTCGGCTAtttg                                           >  2:10415413/1‑139 (MQ=255)

                                                                                             gtCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCg                                          >  1:8482080/1‑140 (MQ=255)

                                                                                             gtCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCg                                          <  1:1948631/140‑1 (MQ=255)

                                                                                              tCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCgg                                         <  1:1743009/140‑1 (MQ=255)

                                                                                              tCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCgg                                         <  2:7256404/140‑1 (MQ=255)

                                                                                                 tttCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGGAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCggtgg                                      <  2:470580/140‑1 (MQ=255)

                                                                                                       aTACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCaaagaa                                                                                                                        >  2:7912966/1‑52 (MQ=255)

                                                                                                       aTACTCATTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAAtt                                <  1:8756295/140‑1 (MQ=255)

                                                                                                             aTTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAAtt                          >  1:6240053/1‑140 (MQ=255)

                                                                                                             aTTACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAAtt                          >  1:5131066/1‑140 (MQ=255)

                                                                                                               tACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTg                        >  2:3911276/1‑140 (MQ=255)

                                                                                                               tACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTg                        >  2:7707820/1‑140 (MQ=255)

                                                                                                               tACAGGTGTTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTg                        >  2:6160790/1‑140 (MQ=255)

                                                                                                                    gtgtTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTggcggc                   <  2:7464331/140‑1 (MQ=255)

                                                                                                                     tgtTAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCa                  <  2:7060527/140‑1 (MQ=255)

                                                                                                                       ttAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  1:911760/1‑140 (MQ=255)

                                                                                                                       ttAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATc                >  2:3529263/1‑140 (MQ=255)

                                                                                                                        tAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCg               <  1:747680/140‑1 (MQ=255)

                                                                                                                        tAATCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCg               <  2:329901/140‑1 (MQ=255)

                                                                                                                         aaTCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAAt                                                                                                     >  2:10228495/1‑53 (MQ=255)

                                                                                                                         aaTCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTTTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              <  2:9099955/140‑1 (MQ=255)

                                                                                                                         aaTCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATggcggc                                                 >  1:4908330/1‑105 (MQ=255)

                                                                                                                         aaTCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              >  2:10647080/1‑140 (MQ=255)

                                                                                                                         aaTCA‑TTTTTTTGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGa              <  1:5629643/140‑1 (MQ=255)

                                                                                                                                tttgtgggCCGCTTCTGTTAGCAAAGAATGCAGGAAAGCATCATAATCAACGTAGAATCTGGTTGTTGCTGGTTCAGTGTTTAGATCACCGCTGGCGGCTATTTCGGGTGTTAATGTCAAATTGGCAGCATCGATATCtg        >  2:6732697/7‑139 (MQ=255)

                                                                                                                                     tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTc   <  2:4819176/140‑1 (MQ=255)

                                                                                                                                     tGGCCGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTc   <  2:1792459/140‑1 (MQ=255)

GTTTTTAAATGTGATAAACCTTATACCTTGAGTACATGGAGAGAATAATCGCTTATCAGACTTCAGGTACCTCAAAGAGTGTCTTTTTACCGTGTCTTTTCTGATACTCATTACAGGTGTTAATCA‑TTTTTTTGGCTGCTTCTTTTAGCAAAGAAAGCATTAAATCATCATAATCAACGTAGAATCTGGTTGTTTCAGTTTCCGTTTTTATCTCACCGATGGCGGCTATTTCGGTGGTTAATTTCAATTTGGCGGCATCGATATCTTTATTCG  >  NZ_CP025268/1236526‑1236798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`