BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,292,848	A→T	noncoding (11/132 nt)	CXP41_RS06830 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,292,848	0	A	T	77.3%	320.4 / 187.4	366	noncoding (11/132 nt)	CXP41_RS06830	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (60/20); new base T (46/227); total (115/251)
Fisher's exact test for biased strand distribution p-value = 6.35e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 369 total aligned reads displayed.

TTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTC  >  NZ_CP025268/1292711‑1292987

ttattTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATg                                                                                                                                           <  2:7135759/140‑1 (MQ=255)

 tattTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                                                          >  1:9841231/1‑140 (MQ=255)

  attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  1:7061171/140‑1 (MQ=14)

  attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  2:9336444/140‑1 (MQ=14)

  attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  1:7593080/140‑1 (MQ=14)

  attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  2:2009866/140‑1 (MQ=14)

  attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  2:2789208/140‑1 (MQ=14)

  attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  2:6874830/140‑1 (MQ=14)

  attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                                                         <  2:3953754/140‑1 (MQ=255)

  attGAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                                                         <  2:8079737/140‑1 (MQ=11)

   tttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGc                                                                                                                                        >  2:3726989/1‑140 (MQ=11)

    ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                                                       <  1:5264221/140‑1 (MQ=11)

    tgaaGGCAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                                                       <  2:9890441/138‑1 (MQ=11)

    tgaaGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                                                       <  1:1924172/138‑1 (MQ=11)

    tcacgtaaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGAGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCACAAGTCGAACCATGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                                                       <  1:688601/134‑1 (MQ=11)

      aaGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGa                                                                                                                                     <  2:2199950/140‑1 (MQ=11)

      aaGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGa                                                                                                                                     <  1:4830552/140‑1 (MQ=11)

             cGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTATCTCGCTGTCGCTCCAGTCGAACCTTCGTCGAAGCTTCTCATCCTGCCCCTCGTGGGCAGACTAGTTg                                                                                                                              >  2:10805048/1‑140 (MQ=11)

                                    cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGACAGCGGCAACGCTCTCTCGCTGGCGCTAAAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTGTGAGAATTc                                                                                                       >  2:8402299/1‑140 (MQ=11)

                                      cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCg                                                                                                     <  2:4667181/140‑1 (MQ=11)

                                        gcgcggcgcgCGTCGCCCTTCGGGTCGGGGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGACGCTTCTCATCCGTCCCCGCTGGGGCAGAATATTTGATTGCGGATTCGTGTGAGAAGTCCggg                                                                                                   <  2:8585051/140‑1 (MQ=11)

                                              cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCtttt                                                                                             <  2:6652333/140‑1 (MQ=11)

                                              cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCAAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCCCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCtttt                                                                                             >  1:6449607/1‑140 (MQ=11)

                                               gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTg                                                                                            <  1:2122952/140‑1 (MQ=11)

                                                 gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGaa                                                                                          <  1:6394186/140‑1 (MQ=11)

                                                  cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGaaa                                                                                         <  2:5577636/140‑1 (MQ=11)

                                                    tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGt                                                                                       <  1:6644863/140‑1 (MQ=11)

                                                      gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGa                                                                                     <  1:7498424/140‑1 (MQ=11)

                                                       cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAt                                                                                    <  1:10092022/140‑1 (MQ=11)

                                                       cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAt                                                                                    <  2:7995628/140‑1 (MQ=11)

                                                        ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtg                                                                                   <  2:8881303/140‑1 (MQ=11)

                                                        ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtg                                                                                   <  1:896637/140‑1 (MQ=11)

                                                         cTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtgg                                                                                  <  1:2217507/140‑1 (MQ=11)

                                                            cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                                                               <  1:8594715/140‑1 (MQ=11)

                                                             gggTCGTTGCCTGCGGCAACGCTCTCTTGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                                                              <  2:8457204/140‑1 (MQ=11)

                                                             gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATTTTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                                                              <  2:6577311/140‑1 (MQ=11)

                                                             gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                                                              <  2:4592991/140‑1 (MQ=11)

                                                                tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCACGTCGAACCTTGGTCGAAGCGTCTCAGCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGAGGTggg                                                                           <  2:6374266/140‑1 (MQ=11)

                                                                tCGTTGCCTGCGGCAACGCTCTCGCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggg                                                                           <  2:2649498/140‑1 (MQ=11)

                                                                gcGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggg                                                                           <  2:7708706/139‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  2:7515181/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  2:4182154/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:264080/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:4238364/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:996436/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:4659132/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:9318360/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:1633366/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                                                         <  1:5582851/140‑1 (MQ=11)

                                                                  gTTGCCTGCGGCAACGCACACTCGCGGGCGCTCAAGTCGAAACGTGGTCGAAGCTTATCATCCATACCCCCCTGGGCAGAATATTTGATTGCGGATTCGATTGAGAATTCCGGGGCTTTTTAAAGTGAtggtggtggtgg                                                                         >  1:587465/1‑140 (MQ=11)

                                                                    tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtggt                                                                              >  2:10421289/1‑133 (MQ=11)

                                                                    tGCCTGCGGCAACGCACACTCGCTGGCGCACAAGTCGAACCTTGGTCGAACCTTCTCAACCATCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTTGTGAATTCAGGGGATTTTTAAAGTGATGGTGGTGGGGGaa                                                                       >  1:7293296/1‑140 (MQ=11)

                                                                       cTGCGTCATCGCTCTCTCGCTGGCTCTCACGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGAGTCGTTTGAGAATTCCGGGGCTTTTGAACGTGATGGTGGTGGGGGAAGGa                                                                    <  2:6726557/140‑1 (MQ=11)

                                                                       cTGCGGCTACGCTCTCTCGCTGGCTCTCATGTCGAAAGTTGGTCGTATCTTCTGATCCTTACCCGTTTGGGCAGATTAATTTATTTCGGATTCGTTTGAGAATTCCTGTTATTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  2:7816722/140‑1 (MQ=2)

                                                                       cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGCACCATGGTCGAAGCTTCTCCTCCTTCCCCGCGTGGGCAGAATATTTGATTGCGGATACGTTGTAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  2:3600097/140‑1 (MQ=11)

                                                                       cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  1:10531940/140‑1 (MQ=11)

                                                                       cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCTCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  2:8275232/140‑1 (MQ=11)

                                                                       cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                                                    <  2:5702391/140‑1 (MQ=11)

                                                                            gCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                                                               <  1:7530449/140‑1 (MQ=14)

                                                                                    ctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTg                                                       <  2:10275515/140‑1 (MQ=14)

                                                                                     tctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCAGCCGTCCCCTCTTGGGCAGAATAATTCATTTCGGATTCGTTTGAGAATTCCGTGGCTATAGAACGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                                                      <  2:10054238/140‑1 (MQ=14)

                                                                                     tctcGCTGACGCTCGAGCCGAAACTTAGTCGTAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                                                      <  2:6041477/140‑1 (MQ=11)

                                                                                      ctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcg                                                     <  1:9541505/140‑1 (MQ=14)

                                                                                      ctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcg                                                     >  2:1255705/1‑140 (MQ=14)

                                                                                       tcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGGTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgc                                                    >  1:5219409/1‑140 (MQ=17)

                                                                                        cGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                                                   <  2:2439275/140‑1 (MQ=11)

                                                                                        cGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                                                   <  1:4121940/140‑1 (MQ=14)

                                                                                           tGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt                                                <  2:1761672/140‑1 (MQ=14)

                                                                                           tGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt                                                >  1:2439275/1‑140 (MQ=14)

                                                                                            ggCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGa                                                                        <  1:3532884/115‑1 (MQ=14)

                                                                                            gacgcTCGAGCCGAACCTTAGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc                                               <  1:5358440/138‑1 (MQ=11)

                                                                                             gcgcTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                                              <  2:4780929/140‑1 (MQ=11)

                                                                                             gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                                              <  1:655953/140‑1 (MQ=14)

                                                                                             gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                                              >  1:1321417/1‑140 (MQ=14)

                                                                                             gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg                                              <  1:3974195/140‑1 (MQ=14)

                                                                                             acgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt                                                 <  2:7740572/136‑1 (MQ=255)

                                                                                               gcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAGTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGcc                                            >  1:8264017/1‑140 (MQ=11)

                                                                                               gcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGcc                                            <  2:10596521/140‑1 (MQ=14)

                                                                                                   gAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                                        <  1:10024510/140‑1 (MQ=255)

                                                                                                   gAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc                                        >  1:10592541/1‑140 (MQ=255)

                                                                                                      tcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCggg                                     <  1:3102590/139‑1 (MQ=255)

                                                                                                         aaCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCGTCGCTTCGCTCCTCACCCTTCGGGCCg                                  >  2:10154473/1‑140 (MQ=14)

                                                                                                         aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCg                                  >  2:7850831/1‑140 (MQ=255)

                                                                                                              tggtCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGcc                             <  1:3391382/137‑1 (MQ=255)

                                                                                                              tagtCGAAGCTTCTGATCCGTCCCCGCCGGGGCAGATGCTTTGATTGCGGATGCGCTTGAGCGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCCTCGCCCTTCGGGTCGTTGcc                             <  1:7866115/137‑1 (MQ=11)

                                                                                                              tagtCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGcc                             <  1:551375/137‑1 (MQ=255)

                                                                                                               agtCGAAGCTTCTNATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                            >  1:9406326/3‑140 (MQ=255)

                                                                                                               agtCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                            >  1:10334780/3‑140 (MQ=255)

                                                                                                                      gCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCaa                     >  2:4040676/1‑140 (MQ=255)

                                                                                                                       cTTCTCCTCCCTGCCCGCTTGGGCAGACTTTTTTATCGCGGATTCGTATGACAATTCATGGGCTAATGAATGTGATGGTGGAGGGGGAAGGATTACTCTGCGCTGCGCGCTTCGCCCTTCGGGCCGTTGCCAGTGGCAAc                    <  2:7049247/140‑1 (MQ=2)

                                                                                                                           tcATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctc                >  2:9714525/1‑140 (MQ=255)

                                                                                                                               ccTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAGGGTGGTGGGGGACGGATTAATCAGCGCGGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCACAGCtctctc            >  2:6308258/1‑140 (MQ=11)

                                                                                                                                 ttCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGc          >  1:3787419/1‑140 (MQ=255)

                                                                                                                                 ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGc          >  1:6071306/1‑140 (MQ=255)

                                                                                                                                 ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGc          >  2:3406264/1‑140 (MQ=255)

                                                                                                                                  tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCt         >  1:7092447/1‑140 (MQ=255)

                                                                                                                                   ccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTg        >  1:8050633/1‑140 (MQ=255)

                                                                                                                                   ccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCATTCGGGTCGTTGCCGGCGGCAACGCACGCTCGcgg        >  1:4479144/1‑138 (MQ=14)

                                                                                                                                       gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCCAAGCTCTCACGCTGgcgc    >  1:6792130/1‑140 (MQ=11)

                                                                                                                                         aTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTc  >  2:5240106/1‑140 (MQ=255)

TTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTC  >  NZ_CP025268/1292711‑1292987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`