BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,001	A→G	noncoding (36/132 nt)	CXP41_RS06835 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,001	0	A	G	99.7%	965.0 / NA	341	noncoding (36/132 nt)	CXP41_RS06835	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (156/183); minor base T (1/0); total (158/183)
Fisher's exact test for biased strand distribution p-value = 4.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 341 total aligned reads displayed.

TGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGT  >  NZ_CP025268/1292866‑1293134

tGCGGATTCGTTTGAGCATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   <  1:4110614/140‑1 (MQ=255)

tGCGGATTCGTTTGAGATTTCGGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   <  2:5785070/140‑1 (MQ=14)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGTTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   <  1:5473214/140‑1 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   >  1:7445819/1‑140 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   <  1:6408483/140‑1 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   >  2:9170527/1‑140 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   <  2:8724000/140‑1 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   >  2:8472095/1‑140 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   >  2:1516091/1‑140 (MQ=255)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGAGAGCTTCTCCCTTCTGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACAATAGTCGa                                                                                                                                   >  2:1852225/1‑140 (MQ=14)

tGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACACAGCGCTGCGCGCTACGCCCAACGGGTCGTTTCCAGCCGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   >  1:8365421/1‑140 (MQ=14)

tGCGGATTCGATTGAGAATTCCGTGGATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGa                                                                                                                                   <  1:7567326/140‑1 (MQ=255)

 gCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGaa                                                                                                                                  <  1:4922428/140‑1 (MQ=255)

  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                                 >  1:7386896/1‑140 (MQ=255)

  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                                 >  1:4369155/1‑140 (MQ=255)

  cGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAAAGCTCTCACTCTGACCCACAAACCGATCCTTCGTCGAAg                                                                                                                                 >  2:4594397/1‑140 (MQ=14)

  cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTAGTTGCCAGCGGCAACGATCTCTCCCTGACGCTCGAAACGAACCATAGTCGAAg                                                                                                                                 >  2:1033708/1‑140 (MQ=11)

    gATTCGTTTGAGTATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                                               <  1:3425515/140‑1 (MQ=255)

    gATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                                               <  1:7257339/140‑1 (MQ=255)

      gtCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTtc                                                                                                                             <  2:6821666/139‑1 (MQ=255)

       tCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGTGGCAACGCTCTCTCGCTTTCGCTCGATTCGAACCTTAGTCGAAGGTtct                                                                                                                            <  1:2199109/140‑1 (MQ=14)

         gtttGAGAATTCCGGGGCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                                          <  2:2714503/138‑1 (MQ=255)

          tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                         <  1:1460011/139‑1 (MQ=255)

                 aTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTccc                                                                                                                  <  2:1907472/140‑1 (MQ=38)

                     cGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCa                                                                                                              >  1:8104341/1‑140 (MQ=25)

                         tcTTTTGAAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                          <  2:9384820/139‑1 (MQ=12)

                         ggttttGTCAGTGTTGGTGGTGTGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                          <  1:2252146/138‑1 (MQ=11)

                         gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                          <  1:7035929/140‑1 (MQ=18)

                          cTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                         >  2:8062404/1‑140 (MQ=17)

                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                        <  1:5744650/140‑1 (MQ=17)

                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                        <  1:8278054/140‑1 (MQ=17)

                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                        <  1:10080067/140‑1 (MQ=17)

                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                        <  1:10156650/140‑1 (MQ=17)

                           ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCa                                                                                                        <  1:6850186/140‑1 (MQ=17)

                             ttGAAAGTGATGGTGGTGGGGGAATGATTACTCAGAGCTGCGAGCTTCTCCCTTAGGGTCGTTTCATGCGGCAACTCTCTCTCGCTTACGCTCGATAAGTACATTAGTAGAAGATTCTCATCCTTCCCCGCTTGGTCAGa                                                                                                      >  2:5598086/1‑140 (MQ=2)

                              tggaaGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGaa                                                                                                     <  1:9629779/137‑1 (MQ=17)

                                        ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTg                                                                                           <  1:10738938/140‑1 (MQ=21)

                                                    aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGATTCTCATACTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                               >  2:9773532/1‑140 (MQ=11)

                                                      gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  1:4053463/140‑1 (MQ=255)

                                                      gATTACTCAGCGCTGCGCGCGTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCACTCTCTCTGACGCTCGGGACGACACTTAGGCGAAGATTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  2:442365/140‑1 (MQ=14)

                                                         tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGATGCTTCTCAGCCTTGCCCGCAGGGGCAGTATATTTGATTGCGGATTCGGTGGGGAGt                                                                          >  2:9305095/1‑140 (MQ=11)

                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAATATTTAATTGCGGCTTCGTTGGGAAGTTc                                                                        >  2:8793560/1‑140 (MQ=14)

                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGGTTCTCATCCTTCCCCGCATGGGCCCAATATTTAATTGCGGATTAGTTTGGAAGtta                                                                        >  2:1219087/1‑139 (MQ=11)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                   <  1:4596808/140‑1 (MQ=255)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGa                                                                   >  2:515553/1‑140 (MQ=11)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTGTCGCTGGCGCGCGAGGCGAACCTTGGTCGATGCTTCTCATCCTTCCCCGCCGGGGCCGAGTAGTTTGTTGGGGATTGGGTGGGAGGTTCAgggg                                                                   >  2:3869613/1‑139 (MQ=2)

                                                                 gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGAc                                                                  >  2:3634132/1‑140 (MQ=255)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                              >  1:8321715/1‑140 (MQ=255)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTAATTGCGGATTAGTTGGGAAGGTCAGGGACtttt                                                              >  1:10796350/1‑140 (MQ=14)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTAGTACCTTGGTAGAAGCTTCTCATACTTCACCGCATTGGCCGAATATGTAATTGCGGATTAGTTGGGAAGTTCAGGGGCtttt                                                              >  2:2528714/1‑140 (MQ=14)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGCACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGTGCAGAATATTTTATTTCGGATTCGTTTGGAAGTTCAGGGACgttt                                                              >  2:4066772/1‑140 (MQ=11)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTGGGGAGTTCAGGGACtttt                                                              >  2:7175162/1‑140 (MQ=11)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCAAACTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTAAGGGACtttt                                                              >  2:2392746/1‑140 (MQ=14)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCGCGAGGCGAACCGTGGTCGAAGCGTCTCATCCTTCCCCGCCTGGGCAGGATATTTAATTGGGGTTTGGTTGGGGAGGTGAGGGTCtttt                                                              >  1:1748663/1‑140 (MQ=11)

                                                                      gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTGGGGACGTTCAGGGCCTTTTg                                                             <  2:300216/140‑1 (MQ=14)

                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                           <  1:8927753/140‑1 (MQ=255)

                                                                        gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaa                                                           <  2:5955799/140‑1 (MQ=255)

                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGaaa                                                          <  1:8017689/140‑1 (MQ=255)

                                                                          ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAATCTTCTCATCCATCCCCCCATGGGCCGAATATTTAATTTCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAg                                                         >  1:8640348/1‑140 (MQ=14)

                                                                            cGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGGTTCGGTGGGTAGTTCAGGGACGTTTGTAAGTg                                                       >  1:9975733/1‑140 (MQ=11)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                                      <  2:536139‑M1/140‑1 (MQ=255)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATTGGCAGAATATTGAATTGCGGATACGTTGGGAAGTTCAGGGCCGTTTTATAGTgg                                                      >  2:9026560/1‑139 (MQ=14)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                                      >  2:8270480‑M1/1‑140 (MQ=255)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                                      <  1:1343630‑M1/140‑1 (MQ=255)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGA                                                      <  1:3367857‑M1/140‑1 (MQ=255)

                                                                              cccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAT                                                     <  1:3026680‑M1/140‑1 (MQ=255)

                                                                                  tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTG                                                 <  2:6629786‑M1/140‑1 (MQ=255)

                                                                                  tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATTGTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTGTTGAAAGTGATGGTG                                                 >  2:6295669‑M1/1‑140 (MQ=255)

                                                                                    gggTCGTTGCCGGCGGCAACGCGCTCTCGCTGACGCTCGAGCCCAACCTTAGTCGAAGCTTCTCAACCTTCCCCGCAAGGGCCGAATATTTGATTGCGGCTTCGGTTGGGAGTTCAGGGACGTTTGAAAGTGAtggtggg                                               >  1:3747992/1‑139 (MQ=255)

                                                                                        cGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGCATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGTAAGTGATGGGGGTGGGG                                           >  2:3968874‑M1/1‑140 (MQ=255)

                                                                                          gtgCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGATTCTCATCCTTACCCGCTTGGGCAGAATATTTAATTGCGGATGCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGA                                         <  2:5888686‑M1/139‑1 (MQ=255)

                                                                                           tGCCTGCGGCAAAGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGTCTCTTCTCATCCTTACCCGCATGGGCAGAATATTTATTTGCGGATTAGTTGGGTAGTTCAGGGACTTTTGATAGTGTTGGTGGTGGGGGac                                        >  2:2210730/1‑139 (MQ=11)

                                                                                                 cGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCATCCCCCCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTTTAAGTGATGGTGGTGGGGGAAGGAttc                                  >  1:6716853/1‑139 (MQ=14)

                                                                                                    cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTGAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgac                               >  2:7546694‑M1/1‑136 (MQ=255)

                                                                                                       cGCTCTCTCGCTGGCGCTCGAGTCGACCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaacct                            >  1:9344043‑M1/1‑133 (MQ=255)

                                                                                                       cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                 <  1:6791079/87‑1 (MQ=11)

                                                                                                       cGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaacct                            >  2:1373214‑M1/1‑133 (MQ=255)

                                                                                                            tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaa                       >  1:6356590‑M1/1‑128 (MQ=255)

                                                                                                             ctcGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGCTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaag                      <  2:10749836‑M1/140‑14 (MQ=255)

                                                                                                             ctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaag                      <  1:4834126‑M1/140‑14 (MQ=255)

                                                                                                              tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagt                     >  2:3518683‑M1/1‑126 (MQ=255)

                                                                                                                gCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATTTTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGTTGGTGGTGGGGGAAGGATTcgaaccttcgaagtcg                   >  2:1670869‑M1/1‑124 (MQ=255)

                                                                                                                 cTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                             <  1:4217242/81‑1 (MQ=14)

                                                                                                                  tggcgctCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTACCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgat                 >  1:2826996‑M1/4‑122 (MQ=255)

                                                                                                                  tggcgctCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgat                 <  1:1628235‑M1/137‑19 (MQ=255)

                                                                                                                  tggcgctCAAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgat                 <  1:10318900‑M1/137‑19 (MQ=255)

                                                                                                                   ggcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatg                <  1:8809337‑M1/138‑20 (MQ=255)

                                                                                                                   ggcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatg                >  1:10832561‑M1/3‑121 (MQ=255)

                                                                                                                   ggcGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGAt                                                                                      >  2:4909580/3‑70 (MQ=11)

                                                                                                                    gcgCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatga               >  1:7691672‑M1/2‑120 (MQ=255)

                                                                                                                     cGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgac              <  1:3634132‑M1/140‑22 (MQ=255)

                                                                                                                       cTCGATTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTGGTTATTTCCGGTACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg            <  2:4733901‑M1/140‑24 (MQ=255)

                                                                                                                       cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg            <  2:7092447‑M1/140‑24 (MQ=255)

                                                                                                                       cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg            <  1:52073‑M1/140‑24 (MQ=255)

                                                                                                                       cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGCAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacgg            <  2:8979777‑M1/140‑24 (MQ=255)

                                                                                                                         cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgangtcgatgacggca          <  1:5859103‑M1/140‑26 (MQ=255)

                                                                                                                         cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggca          <  2:7785641‑M1/140‑26 (MQ=255)

                                                                                                                             tcgAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagatt      <  1:2513093‑M1/139‑30 (MQ=255)

                                                                                                                              cGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggctgattt     >  1:9277873‑M1/1‑110 (MQ=255)

                                                                                                                                 aCCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagatttaca  <  2:9719704‑M1/140‑34 (MQ=255)

TGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGT  >  NZ_CP025268/1292866‑1293134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`