BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,293,160	G→A	noncoding (55/132 nt)	CXP41_RS06840 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,293,160	0	G	A	76.7%	329.4 / 90.4	203	noncoding (55/132 nt)	CXP41_RS06840	RtT sRNA
Reads supporting (aligned to +/- strand): ref base G (21/20); new base A (29/106); total (57/146)
Fisher's exact test for biased strand distribution p-value = 5.93e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.35e-01

Only 100 of 203 total aligned reads displayed.

TTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCT  >  NZ_CP025268/1293039‑1293286

ttgaTTGCGGATTCGCTGGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGATCTCTCGCTGGCGCGCGAGTCGAACCttg                                                                                                              >  2:1122767/4‑139 (MQ=11)

              gctagagaGTACAGGGACTTATGACAGCGATCGTGGTGGGGGAACGATTACACAGCTCTGCGCTCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCa                                                                                                <  2:6735860/133‑1 (MQ=11)

               cttgagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                               <  2:2117452/134‑1 (MQ=11)

               cttgagaGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                               <  1:1255705/134‑1 (MQ=11)

                      gTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTccc                                                                                        >  2:2652209/1‑140 (MQ=255)

                      gTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAGGATTACTCAGCGCTGAGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCGCATccagccc                                                                                        >  2:399185/1‑135 (MQ=11)

                       ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCACTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATGGTCGAAGCTTCTCATCCTTcccc                                                                                       >  2:9392258/1‑140 (MQ=11)

                       ttCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCAACGCCCTTCGGGGCGTTGCCTGAGGCAACGCTCAATCGGTGTCGCGACATATGAACCTTGCTCGAAGCTCATCATCCtacgac                                                                                       >  2:5203973/1‑135 (MQ=2)

                         cAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCTCTGCGCGCTTCGCCCTTCGGGTCGTTTCAGGCGGCAACGCTAACTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATACTTCCCCGc                                                                                     >  1:3859625/1‑140 (MQ=11)

                         cAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                     <  2:548233/140‑1 (MQ=255)

                         cAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                     <  1:9687649/140‑1 (MQ=255)

                            ggACTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGGGGCACCGCTGTCTCGCTGGCGCTCGGGTGGTAGCTTGGTGGTAGCGTCTCTTCCTTCCCCggcgg                                                                                  >  1:8445141/1‑136 (MQ=11)

                               cTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                               >  2:6898561/1‑140 (MQ=12)

                               cTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGAGCTCGAGTCGCACCGTGGTCGAAGCTTCTCATCCTTCCCCGCCTGGGc                                                                               >  2:6288210/1‑140 (MQ=11)

                                 tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAg                                                                             >  2:10701121/1‑140 (MQ=12)

                                                              tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCTGCGGCAACGCGATCTCGCTGGCGCTCAAGTCGAACCTTGGTAGAAGCTTATCATCCATCCCCGCTTGGGCCGTATATTTTATTGCGGATTGGTTTgagtat                                                >  2:13941/1‑137 (MQ=1)

                                                              tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGAGAGt                                                >  2:904214/1‑140 (MQ=255)

                                                              tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGGAGAATATTTGATTGCGGATTCGCTTGTGAGt                                                >  2:9305132/1‑140 (MQ=255)

                                                                   aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                           <  1:10421074/140‑1 (MQ=21)

                                                                   aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAgg                                           <  1:7304280/140‑1 (MQ=21)

                                                                    gcgcTGCGCGCTTCGCCCTGCGGGTCGTTGCCTGCGCCAACGCTCTCGCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAggg                                          <  1:8862523/140‑1 (MQ=11)

                                                                     cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                         <  1:799986/140‑1 (MQ=255)

                                                                     cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCCTCCTTCCCCGACTGGGCCGAATATTGGATTGCGGATTCGCGTGAGAGATAAgggc                                         >  2:9863713/1‑139 (MQ=11)

                                                                     cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGa                                         >  1:6629786/1‑140 (MQ=255)

                                                                        tGCGCGCTTCGCCGTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCATAGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtt                                      >  1:9414752/1‑140 (MQ=11)

                                                                        tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAACCTTCCCCGCATGGGCACAATATTTGATTGCGGATTCGCTTTAGAGTTCAGGGACtt                                      >  1:9110165/1‑140 (MQ=11)

                                                                        tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGCTTGCGGAATCGCTTGAGAGTTCAGGGACtt                                      >  2:5211877/1‑140 (MQ=255)

                                                                          cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCCACGCTCTCTCGCTGGCGCGCGAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCGCAGGGGCCGACATTTTAATTGGGGCTTGGGTTGGGAGTTCAgggtgtgtg                                    >  1:6189312/1‑134 (MQ=2)

                                                                          cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTGAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAAGATTTGATTGCGGATTCGCTGGGGAGTTCAGGGAcaggt                                    >  2:8331025/1‑136 (MQ=11)

                                                                          cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGTGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                    <  2:9445175/140‑1 (MQ=255)

                                                                          cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGAGAGGTCAGGGACtgtt                                    >  2:2805722/1‑140 (MQ=11)

                                                                             gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                 <  1:9822384/140‑1 (MQ=255)

                                                                             gcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                 <  1:7422760/140‑1 (MQ=255)

                                                                             agacagCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                 <  2:587465/135‑1 (MQ=11)

                                                                              cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                <  2:9474902/140‑1 (MQ=255)

                                                                              cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                <  2:5350242/140‑1 (MQ=255)

                                                                              cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTGAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                <  1:7688306/140‑1 (MQ=255)

                                                                               ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                               <  1:4118618/140‑1 (MQ=255)

                                                                               ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                               <  2:5006426/140‑1 (MQ=255)

                                                                               ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTACCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                               <  1:8352476/140‑1 (MQ=255)

                                                                               ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGACCCTTAGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAAGATTTGATGGGGGATTCGGTTGATAGTTGAGGGGCGTTTGTAAg                               >  1:5679605/1‑140 (MQ=11)

                                                                               ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCAGACGCTCGAGCCGAACCATAGTCGAAGCTTCTCATACTTCCCCCCAAGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTGTGAAAg                               >  1:9707236/1‑140 (MQ=11)

                                                                                 cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTg                             <  2:5760938/140‑1 (MQ=255)

                                                                                  gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTGa                            <  2:8859062/140‑1 (MQ=12)

                                                                                  gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGTATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGa                            <  1:10336865/140‑1 (MQ=255)

                                                                                  gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGa                            <  2:3810006/140‑1 (MQ=255)

                                                                                  gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGa                            <  2:9841231/140‑1 (MQ=255)

                                                                                    ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATGCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtg                          <  1:3129998/140‑1 (MQ=255)

                                                                                     cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtgg                         <  1:1753104/140‑1 (MQ=11)

                                                                                     cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtgg                         <  1:9228866/140‑1 (MQ=255)

                                                                                      ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCTCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggt                        <  1:34518/140‑1 (MQ=255)

                                                                                      ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggt                        <  1:452376/140‑1 (MQ=255)

                                                                                      ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggt                        <  2:4906569/140‑1 (MQ=255)

                                                                                       tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtg                       <  1:3640022/140‑1 (MQ=255)

                                                                                        cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCACATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGAtggtgg                      <  1:10690190/140‑1 (MQ=255)

                                                                                         tgttCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                     <  1:5158958/137‑1 (MQ=11)

                                                                                         gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCTTCGAAGCTTCTCAGCCTTCCCCGCATGGGCAGAATTTTTGATTGCGGATTCGATTGAGAGTTCCGGGACTTTTGAAAGTGAtggtggt                     <  2:251970/140‑1 (MQ=11)

                                                                                         gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCATAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                     <  1:3115099/140‑1 (MQ=255)

                                                                                         gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                     <  2:6008301/140‑1 (MQ=255)

                                                                                         gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                     <  1:10443782/140‑1 (MQ=255)

                                                                                         gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                     <  2:4369155/140‑1 (MQ=255)

                                                                                         gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                     <  2:4727655/140‑1 (MQ=255)

                                                                                         gggTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggt                     <  1:6715523/140‑1 (MQ=255)

                                                                                            tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                  <  2:75465/140‑1 (MQ=255)

                                                                                            tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                  <  2:3191632/140‑1 (MQ=255)

                                                                                            tCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                  <  1:6495817/140‑1 (MQ=255)

                                                                                              gtggCCCGCGGCACCGCTCTCGCGCTGCCCCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGAGTGCGGCTTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  2:8631081/137‑1 (MQ=11)

                                                                                              gtcgCCCGCGGCAACGCTCTCTCGCTGTNGCTCGAGCCGAACCTTAGTCGAAGCTTCTCTTACTTCCGCGGTTGGGCAGAATTTTTGATTGCGGATTCGCTTGAGAGTTCCGGTACTTTTGAAAGTGATGGTGGTggggg                <  1:5880433/137‑1 (MQ=11)

                                                                                              gTTGCCTGCGGCTACGCTCTCTCGCTGAAGCTCGCGCCGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGTATTCGATTGAGAGTTCAGTGACTTTTGCAAGTGATGGTGGTggggg                <  1:1833481/140‑1 (MQ=11)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGACGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  2:2330508/140‑1 (MQ=255)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAATTGATTGCTGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  2:8259403/140‑1 (MQ=11)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  2:7741069/140‑1 (MQ=255)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGACTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  1:5949911/140‑1 (MQ=255)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  1:2698365/140‑1 (MQ=255)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  1:10805048/140‑1 (MQ=255)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  1:10514686/140‑1 (MQ=255)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  1:3194054/140‑1 (MQ=255)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAACCTTCCCCGCATGGGGCGAAAATTGGATTGCGGATGCGCGTGAGAGATCCGGGAAGTTTGCATCTGGTGGTGGTggggg                >  2:7366909/1‑140 (MQ=11)

                                                                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTGAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  2:3033316/140‑1 (MQ=255)

                                                                                                 gCCTGCGGCATCGCTCTCTAGCTTTCGATCCTGCCGAACCTTCTTCTTAGCTTCTCATCCTTCCCCGTTTTGGCAGAATTTTTGATTGGGGATTCGATTGAGTGTTGAGGGACTTTTGTAAGTGATGGTGGTGGGGGAAg             <  2:7392543/140‑1 (MQ=2)

                                                                                                 gCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg             <  2:1447174/140‑1 (MQ=11)

                                                                                                  ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAgg            <  1:8658972/140‑1 (MQ=255)

                                                                                                   gtGCGGCTACGCACTCTCGCTGAAGCTCGACCCTACCCTTACTCAAAGATTCTGATCCTTCCCCGCATTGGCATAATATTTTATTGCGGCTTCGCTAGAGTTTTCTCTGAATTTTGTAAGTGATGGTGGTGGGGGAAGGa           <  1:3452039/139‑1 (MQ=2)

                                                                                                   cTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa           <  1:8229297/140‑1 (MQ=255)

                                                                                                   cTGCGGCAACGCTCTCTCGCTGACGCTCGCGCCGAACCTTAGTCGCAGCTTCTCAGCCTTCCCCGCATGGGCAGAATATTGGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa           <  2:6185729/140‑1 (MQ=11)

                                                                                                   cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa           <  1:6785952/140‑1 (MQ=255)

                                                                                                   cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa           <  1:3999810/140‑1 (MQ=255)

                                                                                                   cTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAAGATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa           <  2:3752424/140‑1 (MQ=255)

                                                                                                   cTGCGGCAACGCTCTCGCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa           <  2:7515238/140‑1 (MQ=255)

                                                                                                   cTGCGGCAACGCACTCTCTCTGACGCTCGAGCCGAACCGTTGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGAATAATTGATTGCGGATTCGCTTGAGAGTTCATGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGa           <  2:4185288/140‑1 (MQ=11)

                                                                                                        gCAACGCTCTCTCGCTGACGCTCGCGCCGAAGCTGTGTCGCTGCTTCTCATCCGTGCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttact      <  2:7144149/140‑4 (MQ=1)

                                                                                                        gCAACGCTCTCGCGCTGCCGCTCGAGCCGAACCGAAGTCGAAGCTTCTCCTCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttact      <  2:10298323/140‑4 (MQ=11)

                                                                                                         cAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttactc     <  2:5219409/140‑5 (MQ=255)

                                                                                                         cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCCGAATATTTGATTGCGGATTCGCGTGCGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttactc     >  2:2454978/1‑136 (MQ=11)

                                                                                                          aaCGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGATTCTCATCCTTCCCCGCATGGGCAGAATTTTTTATTGCGGATTCGCTTGAGAGTTCATGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttactca    <  1:2092850/140‑6 (MQ=11)

                                                                                                           aCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAttactcag   <  1:8402146/140‑7 (MQ=255)

                                                                                                            cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATCTTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGTAAGTGATGGGGGTGGGGGAAGGAttactcagc  >  1:8588884/1‑133 (MQ=11)

                                                                                                            cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGGGGTGGGGGAAGGAttactcagc  >  1:4687600/1‑133 (MQ=11)

                                                                                                                       tGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAGGGACTTTTGAAAGTGATGGTGGTggggg                >  1:6365428/1‑115 (MQ=11)

                                                                                                                       tGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAGGGACTTTTGAAAGTGATGGTGGTggggg                <  2:6365428/115‑1 (MQ=11)

TTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCT  >  NZ_CP025268/1293039‑1293286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`