BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,576,736	A→G	F43F (TTT→TTC)	gadB ←	glutamate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,576,736	0	A	G	99.7%	1073.3 / NA	319	F43F (TTT→TTC)	gadB	glutamate decarboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (93/225); minor base T (0/1); total (93/226)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

CTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCATCATTGATAATCTGAAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAAC  >  NZ_CP025268/1576600‑1576871

      tGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACa                                                                                                                                <  1:9431249/140‑1 (MQ=255)

        tCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACAtc                                                                                                                              <  1:1820377/140‑1 (MQ=255)

           aTCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGATCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACAtcgtc                                                                                                                           >  2:8377053/1‑140 (MQ=255)

             ccAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTcgc                                                                                                                         >  1:3029252/1‑140 (MQ=255)

             ccAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTcgc                                                                                                                         <  1:9734226/140‑1 (MQ=255)

                  ttttGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCAtt                                                                                                                    >  1:8172184/1‑140 (MQ=255)

                      gTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGt                                                                                                                >  1:9121320/1‑140 (MQ=255)

                        tAATGGATAAATCCATCAATTTGTGGNCATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTNGCGCATTTCGTGc                                                                                                              <  1:5858155/140‑1 (MQ=255)

                        tAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGc                                                                                                              <  1:3532003/140‑1 (MQ=255)

                        tAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGc                                                                                                              <  1:6520291/140‑1 (MQ=255)

                         caTGGAAAAATCCATCCATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAAGTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCa                                                                                                             <  2:10020685/139‑1 (MQ=14)

                         aaTGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCa                                                                                                             <  2:650545/140‑1 (MQ=255)

                         aaTGGATAAATCCATCAATGTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCa                                                                                                             <  1:8480671/140‑1 (MQ=255)

                               cacaTCCATCAATTTGAGGACAATTTCGTCGTCCCAGGTCTGGCAGCAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGAAATAATTCGTCAATGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGaa                                                                                                       <  2:5447858/137‑1 (MQ=11)

                                 aaTCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGaaaa                                                                                                     <  1:2311371/140‑1 (MQ=255)

                                 aaTCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGaaaa                                                                                                     <  1:4976205/140‑1 (MQ=255)

                                       tCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGtttt                                                                                               >  2:5619955/1‑140 (MQ=255)

                                         aatatttgGACATTTTAGTCGTACCAGGTCTTGAAGTTAGTGGCCATGTTCTGACGTGCGTTGCCTTCAAGATATTATTCGTCTTTGATACTCTGGAAATCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGa                                                                                             <  1:5359187/134‑1 (MQ=2)

                                         aaTTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGa                                                                                             <  2:4423369/140‑1 (MQ=255)

                                         aaTTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGa                                                                                             <  1:4774232/140‑1 (MQ=255)

                                           tttGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGAtt                                                                                           <  2:10676171/140‑1 (MQ=255)

                                              gtgGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTg                                                                                        <  2:9177271/140‑1 (MQ=255)

                                              gtgGACATTTACGTCGTCCCAGGTCGGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTg                                                                                        <  2:6687368/140‑1 (MQ=255)

                                                ggACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCg                                                                                      <  1:8680985/140‑1 (MQ=255)

                                                ggACATTTGCGTCGGCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATGGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCg                                                                                      <  2:9675510/140‑1 (MQ=14)

                                                ggACAATTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCg                                                                                      <  2:8112068/140‑1 (MQ=255)

                                                 gACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGa                                                                                     >  2:8801853/1‑140 (MQ=255)

                                                  tcaatttCGACGTCCCAGGTCGGGCATATAGTGTGCACGATCTGTCGTGCGTGGCCATCTTGCTATTCTTCGTCTTTTATTTTCTGGTCTGGGACTTCGTCGAGCAATTCGTGCAGCGGAAAACGTTTTGATTCTGCGAt                                                                                    <  2:4979727/136‑1 (MQ=255)

                                                  aCATTTTCGTCGTCCCAGGTCTGGCCGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATTATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGATTTGATTCTGCGAt                                                                                    <  1:722904/140‑1 (MQ=14)

                                                    aTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAg                                                                                  <  2:4668845/140‑1 (MQ=255)

                                                    aTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAg                                                                                  <  2:2437555/140‑1 (MQ=255)

                                                    aTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAg                                                                                  <  1:1466906/140‑1 (MQ=255)

                                                    aTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAg                                                                                  <  1:2856883/140‑1 (MQ=255)

                                                    aTTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAg                                                                                  <  1:383049/140‑1 (MQ=255)

                                                    aTTTTCGTCGTCCCAGGTCGGGCAGAATGTGGCCAGGTTCTGAAGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGGGCAGCGGCAAACGTTTTGAGTCTGCGATAg                                                                                  <  2:1322808/140‑1 (MQ=11)

                                                              tcCCGGGTCTGGCAGAAAGTGGCCCGGTTCTGACTAGCGTTGCCATCAAGATATAATGCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGGTGTGATTCTGCGATTGTGGAAAttgg                                                                        >  2:5910748/1‑137 (MQ=14)

                                                                       tGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCAcc                                                               <  1:5710293/140‑1 (MQ=255)

                                                                       gggCAGAAAGTGGCCAGGTTCTGACGAGCGGTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCAcc                                                               <  2:5372189/139‑1 (MQ=255)

                                                                        ggCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCa                                                              <  1:443763/140‑1 (MQ=255)

                                                                         gCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCaa                                                             >  1:5517676/1‑140 (MQ=255)

                                                                         gCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCaa                                                             >  1:802949/1‑140 (MQ=255)

                                                                         gCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCa                                                              <  2:9345901/139‑1 (MQ=255)

                                                                                 tGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGaa                                                     <  2:2037264/140‑1 (MQ=255)

                                                                                 tGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGaa                                                     <  1:1969729/140‑1 (MQ=255)

                                                                                 tGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGAGTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGaa                                                     <  1:6108087/140‑1 (MQ=255)

                                                                                 tGGCCAGGTTCTGACGAGCGGTGCCATCAAGATATTATTAGTCATGGATTATCTGGACTGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTGGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGaa                                                     <  2:4924353/140‑1 (MQ=17)

                                                                                 gggCCAGGTTCTGACGCGCGGTGCCATCAAGATATAACTCGTCATTGATAATCTGGACTGCGACATCGGCGCGCATTTCGTGCAGCGGAAAACGTGTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGaa                                                     <  2:9130043/139‑1 (MQ=17)

                                                                                   gCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATc                                                   >  2:10797563/1‑140 (MQ=255)

                                                                                           cTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGtt                                           <  1:4209040/140‑1 (MQ=255)

                                                                                           cTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGtt                                           <  2:2857811/140‑1 (MQ=255)

                                                                                           cTGACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGtt                                           >  2:7443064/1‑140 (MQ=255)

                                                                                             gACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTcc                                         <  1:9346449/140‑1 (MQ=255)

                                                                                             gACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTcc                                         <  2:7561025/140‑1 (MQ=255)

                                                                                             gACGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTcc                                         <  2:1651963/140‑1 (MQ=255)

                                                                                             gACGAGCGTTGCCATCAAGATATAAGTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGAGTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTcc                                         <  2:5391876/140‑1 (MQ=255)

                                                                                              aCGAGCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCg                                        <  2:1484398/140‑1 (MQ=255)

                                                                                                  gCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCt                                    >  1:8398223/1‑140 (MQ=255)

                                                                                                  gCGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCt                                    >  1:4170785/1‑140 (MQ=255)

                                                                                                   cGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCtt                                   >  2:7747560/1‑140 (MQ=255)

                                                                                                   cGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCtt                                   >  2:2489323/1‑140 (MQ=255)

                                                                                                   cGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCtt                                   <  2:3029252/140‑1 (MQ=255)

                                                                                                   cGTTGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCtt                                   >  2:3353257/1‑140 (MQ=255)

                                                                                                   cGTTGCCAACAAGATATAAGTCGTCATTGAGAAACTGGACTGCGACATCGTCGCGCATTTCGAGCAGCGGAAAACGTTTAGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCtt                                   <  2:6087067/140‑1 (MQ=18)

                                                                                                     ttGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTaa                                 >  2:3683315/1‑140 (MQ=255)

                                                                                                     ttGCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTaa                                 <  1:6374589/140‑1 (MQ=255)

                                                                                                       gCCATCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAAt                               <  1:3216498/140‑1 (MQ=255)

                                                                                                          aTCAAGCTATACTTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGAGTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCg                            <  2:5749198/140‑1 (MQ=18)

                                                                                                          aTCAAGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCg                            <  1:1549944/140‑1 (MQ=255)

                                                                                                          aTCAAGATATAAGTCGTCATTGATAATCTGGAAGGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCg                            <  1:10445039/140‑1 (MQ=255)

                                                                                                           tCAAGATATACTTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATATCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGt                           <  2:954479/140‑1 (MQ=255)

                                                                                                             aaGATATAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTa                         >  2:8537826/1‑140 (MQ=255)

                                                                                                                atatAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACtt                      <  1:10226026/140‑1 (MQ=255)

                                                                                                                atatAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACtt                      <  2:7241715/140‑1 (MQ=255)

                                                                                                                atatAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACtt                      >  2:6465292/1‑140 (MQ=255)

                                                                                                                atatAATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCAGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACtt                      >  2:1846759/1‑140 (MQ=255)

                                                                                                                  ataATTCGTCATTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGc                    >  2:1190113/1‑140 (MQ=255)

                                                                                                                         gtcatTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTAt             <  2:4629611/139‑1 (MQ=255)

                                                                                                                         gtcatTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTAt             >  1:5087719/2‑140 (MQ=255)

                                                                                                                         gtcatTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTAt             <  1:788305/139‑1 (MQ=255)

                                                                                                                         gtcatTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTAt             >  2:10212630/2‑140 (MQ=255)

                                                                                                                         gtcatTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTAt             <  2:6364238/139‑1 (MQ=255)

                                                                                                                         gtcatTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTAt             <  1:1387180/139‑1 (MQ=255)

                                                                                                                         gtcatAGATAATCTGGAATGCGACATCGGCGCGCATTTCGTGCAGCGGAAAACGATTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTAt             <  2:10475158/139‑1 (MQ=255)

                                                                                                                         ggcagtGATAATCGGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAATACGTTTTGATACTGCGAGAGTGGAAATAGACTTCGCACCCAACCGTGAATCTAGTAGTTCCGACCTTCAATCCGTTACTTGCTTCTTAt             <  2:2555619/135‑1 (MQ=21)

                                                                                                                          tcatTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATc            >  1:7767897/1‑140 (MQ=255)

                                                                                                                          tcatTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATc            >  1:3325762/1‑140 (MQ=255)

                                                                                                                           catTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGa                                                                                             <  1:4697817/58‑1 (MQ=255)

                                                                                                                           catTGATAATCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATcc           >  2:8054864/1‑140 (MQ=255)

                                                                                                                                  aaTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaa    <  1:9615880/140‑1 (MQ=255)

                                                                                                                                  aaTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaa    <  1:2637208/140‑1 (MQ=255)

                                                                                                                                   ctCTGGAATGCGACATCGTCGCGCCTTTCGTGCAGCGGAAAACGTTGTGATTCTGCGAGAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGGTACTTGCTTCTTATCCATTTTaaa   <  2:7134463/139‑1 (MQ=25)

                                                                                                                                   aTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa   <  1:2094716/140‑1 (MQ=255)

                                                                                                                                   aTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa   <  1:1213930/140‑1 (MQ=255)

                                                                                                                                   aTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa   <  1:1006728/140‑1 (MQ=255)

                                                                                                                                   aTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa   <  1:3273830/140‑1 (MQ=255)

                                                                                                                                   aTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa   <  1:7488815/140‑1 (MQ=255)

                                                                                                                                   aTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa   <  2:2183796/140‑1 (MQ=255)

                                                                                                                                   aTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa   <  2:2634256/140‑1 (MQ=255)

                                                                                                                                   aTCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTaaa   <  2:4175929/140‑1 (MQ=255)

                                                                                                                                    tCTGGAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAAc  <  2:10358005/140‑1 (MQ=255)

CTTCTTTGTCGATCCAGTTTTTGTTAATGGATAAATCCATCAATTTGTGGACATTTTCGTCGTCCCAGGTCTGGCAGAAAGTGGCCAGGTTCTGACGAGCGTTGCCATCAAGATATAATTCATCATTGATAATCTGAAATGCGACATCGTCGCGCATTTCGTGCAGCGGAAAACGTTTTGATTCTGCGATAGTGGAAATAGACTTCGCACCAAAACGTGAATCGAGTAGTTCCGACCTTAAATCCGTTACTTGCTTCTTATCCATTTTAAAC  >  NZ_CP025268/1576600‑1576871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`