BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	160,613	T→C	235 (TAA→TAG)	sfsA ←	DNA/RNA nuclease SfsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	160,613	0	T	C	98.9%	1883.4 / NA	546	235 (TAA→TAG)	sfsA	DNA/RNA nuclease SfsA
Reads supporting (aligned to +/- strand): ref base T (0/1); major base C (300/239); minor base A (3/3); total (303/243)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 576 total aligned reads displayed.

GGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCGCGG  >  NZ_CP025268/160476‑160749

ggggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                          <  1:4471146/140‑1 (MQ=255)

ggggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                          >  1:3237984/1‑140 (MQ=255)

ggggAAAAATGAGGCCGATATAAATAGCAGATGCTTGTCCGGATAGCAATTATCTAAACGTACCACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                          <  2:443193/140‑1 (MQ=255)

  ggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACa                                                                                                                                        <  2:1489291/140‑1 (MQ=255)

  ggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACa                                                                                                                                        <  1:8705061/140‑1 (MQ=255)

  ggAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACa                                                                                                                                        <  1:1802590/140‑1 (MQ=255)

    aaaaaTGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAAt                                                                                                                                      >  1:280234/1‑140 (MQ=255)

      aaaTGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAATTTAACAAGTTACTGACTTTACTAAAATGt                                                                                                                                    >  2:7819297/1‑140 (MQ=255)

      aaaTGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGt                                                                                                                                    >  2:1681048/1‑140 (MQ=255)

        aTGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTaa                                                                                                                                  <  1:10784201/140‑1 (MQ=255)

         tGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAAc                                                                                                                                 <  2:2368110/140‑1 (MQ=255)

                        tatcAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGAtttttt                                                                                                                  <  2:1904538/137‑1 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCCGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                               >  2:4885100/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                               >  1:691881/1‑140 (MQ=255)

                           cAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTAACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                               >  2:9432047/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTCTCTACACGTAACACTTGACCACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                            >  2:5131927/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTTCCACTTGACAACTGTGTGAGGAAAATCGAATTTGCGCAGGCGACAAGAATGTAAATTAACCAGGTACTTGCTTTAAGACAATGTAACAGGCTGTGTTTGTTTAagagg                                                                                                            >  2:6682025/1‑139 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                            >  1:5041868/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                            >  2:3719400/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                            >  1:4711488/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                            >  2:6868897/1‑140 (MQ=255)

                              aTGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGAACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGc                                                                                                            >  2:563983/1‑140 (MQ=255)

                               tGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGcc                                                                                                           <  2:1128609/140‑1 (MQ=255)

                                      cctgATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCtt                                                                                                    <  1:1155020/137‑1 (MQ=255)

                                           tAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcagca                                                                                               <  2:8239493/140‑1 (MQ=255)

                                              cAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGaa                                                                                            >  1:3862873/1‑140 (MQ=255)

                                              cAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGaa                                                                                            >  1:6824739/1‑140 (MQ=255)

                                                aTTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAt                                                                                          >  1:10192820/1‑140 (MQ=255)

                                                aTTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAAt                                                                                          >  1:2085796/1‑140 (MQ=255)

                                                     cttaaCGTCACACTTGACACCTGTGTGAGGAACCGCGTATTTGCGCACGCGACAAGACTGTAAATTACACATTGACTTACTTGAATCCAATGTAACCGGCAGTGATATATTAAGATCCATGCCTTCAGCAGAAATTTCCg                                                                                     <  2:1740977/137‑1 (MQ=255)

                                                        aaCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                               <  1:2634456/111‑1 (MQ=255)

                                                        aaCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAag                                                                                                               >  2:2634456/1‑111 (MQ=255)

                                                        aaCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCtt                                                                                  >  1:9594051/1‑140 (MQ=255)

                                                        aaCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCtt                                                                                  >  1:8996358/1‑140 (MQ=255)

                                                        aaCGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCtt                                                                                  >  1:755455/1‑140 (MQ=255)

                                                             aaCACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGAAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCAGCTTTGTaa                                                                             >  2:7395604/1‑140 (MQ=255)

                                                              acacTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAg                                                                            >  2:1397279/1‑140 (MQ=255)

                                                              acacTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAg                                                                            >  2:9203095/1‑140 (MQ=255)

                                                              aaacTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTTCTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAg                                                                            >  2:1071011/3‑140 (MQ=255)

                                                                acTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGcc                                                                          <  1:935417/140‑1 (MQ=255)

                                                                  ttGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAg                                                                        <  2:8170818/140‑1 (MQ=255)

                                                                  ttGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAg                                                                        >  1:5609742/1‑140 (MQ=255)

                                                                   tGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGa                                                                       <  1:7785528/140‑1 (MQ=255)

                                                                   tGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGa                                                                       <  1:162417/140‑1 (MQ=255)

                                                                     aCAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTcagca                                                                                               <  2:10893352/114‑1 (MQ=255)

                                                                         cTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCt                                                                 <  1:699445/140‑1 (MQ=255)

                                                                          tgtgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑a                                                              <  2:2837423/140‑1 (MQ=255)

                                                                            tgtgAGGAACAGCGTCTTTGCGCCCGCGACCAGAATGTACATTAACCCGTTACTTACTTTACTCCCATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTGCAGCAGACTTTTCCGCTGTGTAAGCCAGACGGTCT‑‑Acc                                                            >  2:6474727/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTttacta                                                                                                                                          >  2:3987571/1‑64 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑Acc                                                            >  1:1469571/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑Acc                                                            >  1:8098062/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑Acc                                                            >  2:7941424/1‑140 (MQ=255)

                                                                            tgtgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑Acc                                                            >  1:633140/1‑140 (MQ=255)

                                                                              tgAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑Acccc                                                          >  2:9701726/1‑140 (MQ=255)

                                                                                aGGAAAAGCGTATTTGCGCACGCGACCAGACTGTCAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑Acccccc                                                        >  2:1030745/1‑140 (MQ=255)

                                                                                   aaaaGCGTATATGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATAATTTAAGAGCCAAGCCTTCTGCAGAAATTTCCGCGTTGGAAGCCAGAATTTCT‑‑ACCCCCctct                                                     >  2:2748351/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGtt                                                  >  2:4147859/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGtt                                                  >  2:7453496/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGtt                                                  >  1:4107174/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGtt                                                  >  2:2252696/1‑140 (MQ=255)

                                                                                      aGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGtt                                                  >  1:9795242/1‑140 (MQ=255)

                                                                                        cGTATTTGCGCACGCGACCAGAATTTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGtt                                                  <  1:83063/138‑1 (MQ=255)

                                                                                         gTATTTGCGCACGCGACCAGAATTTAAATTAACCAGTTACTTAATTTCCTACACTGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATGTCCGCGTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAg                                               <  2:2097064/140‑1 (MQ=255)

                                                                                         gTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAg                                               <  2:10509869/140‑1 (MQ=255)

                                                                                         gTATTTGCGCACGCGAACTGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAg                                               <  1:2239601/140‑1 (MQ=255)

                                                                                            tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCtt                                            <  1:4406023/140‑1 (MQ=255)

                                                                                            tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCtt                                            <  2:5478498/140‑1 (MQ=255)

                                                                                            tttGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCtt                                            >  2:8817043/1‑140 (MQ=255)

                                                                                                 gcACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGAc                                       <  2:342961/140‑1 (MQ=255)

                                                                                                  cACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACa                                      >  2:317923/1‑140 (MQ=255)

                                                                                                   aCGCGACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACaa                                     >  1:3087669/1‑140 (MQ=255)

                                                                                                    cgcgACCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCTACACCCCCCTCTGTTGAGCTTCTGACa                                      >  2:7196438/1‑140 (MQ=255)

                                                                                                        aCCAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGtt                                <  1:10496461/140‑1 (MQ=255)

                                                                                                         ccAGAATGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTg                               <  2:4537796/140‑1 (MQ=255)

                                                                                                             aaTGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGcgcg                           <  1:7390324/140‑1 (MQ=255)

                                                                                                               tGTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCNGTTGAGCTTCTGACAATAGTTGCGCGTa                         >  1:5879784/1‑140 (MQ=255)

                                                                                                                gTAAATTAACCAGTTACTTACTTTACTACACTGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAt                        <  1:3382554/140‑1 (MQ=255)

                                                                                                                gTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAt                        <  1:6004542/140‑1 (MQ=255)

                                                                                                                gTAAATTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTAt                        <  1:6524780/140‑1 (MQ=255)

                                                                                                                  ttattttccAGTTAATTACTTTACTACTATGATATAGGGTTTGTTTTTTTAAGTTCCTTTCCTTGAGCATTATTTTCCGCGTTGTTAGACAGAATTTAT‑‑ACCACCCTATGTTGAGCTTCTGACAATAGTTTCTAGTAttt                      <  2:5296399/133‑1 (MQ=255)

                                                                                                                   aaTTAACCAGTTACTTACTTTACTACAATTTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATGtc                     >  2:7263661/1‑140 (MQ=255)

                                                                                                                    aTTAACCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGAAGAAATTTCCGCTTTGTAAGCCAGAATTTAT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTtct                    <  2:3328596/140‑1 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGcgcg                           >  1:10387866/1‑130 (MQ=255)

                                                                                                                       aaCCAGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCAt                 >  1:4964788/1‑140 (MQ=255)

                                                                                                                           aGTTACTTACTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAt             >  1:6740605/1‑140 (MQ=255)

                                                                                                                             ttacttacTTTACTACAATGTACCCGGCCGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGCGCTTCTGACCATAGTTGCGCGTCTTTCTCATCGAtgt           >  2:6088435/1‑140 (MQ=255)

                                                                                                                             ttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCTCTTTGTAAGCCAGAATATCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGAGCGTATTTCTCATCGAtgt           >  2:10096414/1‑140 (MQ=255)

                                                                                                                             ttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAg                                               <  2:4718116/104‑1 (MQ=255)

                                                                                                                             ttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtgt           >  1:2436943/1‑140 (MQ=255)

                                                                                                                             ttacttacTTTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGGTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGAtgt           >  2:8502388/1‑140 (MQ=255)

                                                                                                                               acttacttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTgg         >  1:3611058/1‑140 (MQ=255)

                                                                                                                               acttacttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTGGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTgg         >  1:4631736/1‑140 (MQ=255)

                                                                                                                                 ttactttactACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcg       <  1:10002424/140‑1 (MQ=255)

                                                                                                                                   acttTACTACACTGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  1:8541934/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  2:5967632/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  1:5156234/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  1:5356936/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  2:3523430/140‑1 (MQ=255)

                                                                                                                                   acttTACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGgcgcg     <  1:7240897/140‑1 (MQ=255)

                                                                                                                                     tttACTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGcgcgcg   <  1:9166885/140‑1 (MQ=255)

GGGGAAAAATGAGGCCGCTATAAATAGCAGATGCTTTTCCGGATAGCAATTATCTAAACGTAACACTTGACAACTGTGTGAGGAAAAGCGTATTTGCGCACGCGACCAGAATGTAAATTAACCAGTTACTTACTTTATTACAATGTAACCGGCAGTGATTTTTTAAGAGCCATGCCTTCAGCAGAAATTTCCGCTTTGTAAGCCAGAATTTCT‑‑ACCCCCCTCTGTTGAGCTTCTGACAATAGTTGCGCGTATTTCTCATCGATGTGGCGCGCGG  >  NZ_CP025268/160476‑160749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`