BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,915,287	C→T	P67S (CCC→TCC)	yebQ →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,915,287	0	C	T	99.4%	1759.0 / NA	462	P67S (CCC→TCC)	yebQ	MFS transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (219/239); minor base G (1/2); total (220/242)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 500 total aligned reads displayed.

TTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGT  >  NZ_CP025268/1915150‑1915423

ttGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATctcc                                                                                                                                        <  1:4881531/140‑1 (MQ=255)

 tgttATTGGTATTTCGATGGCAGTCCTTGACGGCGCAATCGCCAACGTCGCCCTTCCAACAATCGCCACTGAACTTCATTCCAAGCCAGCAAGTTCCATCTGGGTAGTGAAAGCCTATCAAATCGCCATTGTCATctccc                                                                                                                                       >  2:460799/1‑140 (MQ=255)

 tgtgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAAGGCCTATCAAATCGCCATTGTCATctccc                                                                                                                                       >  1:7839205/1‑140 (MQ=255)

 tgtgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATctccc                                                                                                                                       >  1:883210/1‑140 (MQ=255)

 tgtgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATctccc                                                                                                                                       >  2:1995945/1‑140 (MQ=255)

 tgtgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATctccc                                                                                                                                       >  2:5129424/1‑140 (MQ=255)

 tgtgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCAGTGTCATctccc                                                                                                                                       >  1:6729433/1‑140 (MQ=255)

  gtgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCt                                                                                                                                      <  1:899248/140‑1 (MQ=255)

   tgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTg                                                                                                                                     >  2:9819161/1‑140 (MQ=255)

   tgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTg                                                                                                                                     >  1:9465932/1‑140 (MQ=255)

   tgATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTg                                                                                                                                     >  1:4658282/1‑140 (MQ=255)

     aTTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGct                                                                                                                                   <  2:3513363/140‑1 (MQ=255)

     aTTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGct                                                                                                                                   >  2:3808611/1‑140 (MQ=255)

      ttGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGctc                                                                                                                                  >  2:2242627/1‑140 (MQ=255)

      ttGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGctc                                                                                                                                  <  1:6908872/140‑1 (MQ=255)

        ggTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCtctc                                                                                                                                >  1:7249120/1‑140 (MQ=255)

        ggTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCtctc                                                                                                                                <  1:6380998/140‑1 (MQ=255)

        ggTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCtctc                                                                                                                                <  1:4522191/140‑1 (MQ=255)

            tttCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCtcgttt                                                                                                                            <  1:2615775/140‑1 (MQ=255)

             ttCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCtcgtttt                                                                                                                           >  2:5502944/1‑140 (MQ=255)

                   ggCCGTCCTTGACGGCGCACTCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTc                                                                                                                     <  2:9704621/140‑1 (MQ=255)

                       gTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTggg                                                                                                                 <  1:9263347/140‑1 (MQ=255)

                        tCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCCGCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTGTTCGTTTCTGGGc                                                                                                                >  2:1219613/1‑140 (MQ=255)

                        tCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGc                                                                                                                >  1:5087604/1‑140 (MQ=255)

                         ccTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                               <  1:7045445/140‑1 (MQ=255)

                         ccTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCg                                                                                                               <  1:7720824/140‑1 (MQ=255)

                           ttGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGat                                                                                                             <  1:3099143/140‑1 (MQ=255)

                               cGGCGCAAACGCCAACGTCGCCCTGCCACCAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCGGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCGCCCTGCTCTCGTTTGCGTTTCTGGGCGATATGt                                                                                                         <  2:7393762/140‑1 (MQ=255)

                                 gcgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGttt                                                                                                       <  2:10631970/140‑1 (MQ=255)

                                 gcgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGttt                                                                                                       <  2:9132262/140‑1 (MQ=255)

                                 gcgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGttt                                                                                                       <  1:1172852/140‑1 (MQ=255)

                                  cgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTg                                                                                                      >  2:8424902/1‑140 (MQ=255)

                                  cgcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTg                                                                                                      >  1:1630899/1‑140 (MQ=255)

                                   gcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTgg                                                                                                     >  1:3811658/1‑140 (MQ=255)

                                   gcAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTgg                                                                                                     <  2:3334442/140‑1 (MQ=255)

                                    cAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGc                                                                                                    >  2:15398/1‑140 (MQ=255)

                                           cAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                             <  1:10041733/140‑1 (MQ=255)

                                           cAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGAc                                                                                             <  2:9302313/140‑1 (MQ=255)

                                            aaCGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACg                                                                                            >  2:10901027/1‑140 (MQ=255)

                                              cGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGACCGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTa                                                                                          <  2:8839537/140‑1 (MQ=255)

                                              cGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTa                                                                                          >  1:10290046/1‑140 (MQ=255)

                                              cGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTa                                                                                          >  1:2384651/1‑140 (MQ=255)

                                               gTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTAt                                                                                         >  2:8719672/1‑140 (MQ=255)

                                                 cGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTAttt                                                                                       >  1:4233287/1‑140 (MQ=255)

                                                   cccTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTtat                                                                                     >  2:580096/1‑140 (MQ=255)

                                                   cccTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTtat                                                                                     >  1:10692666/1‑140 (MQ=255)

                                                        ccAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGTGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATg                                                                                <  1:9631006/140‑1 (MQ=255)

                                                        ccAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATg                                                                                >  2:9597346/1‑140 (MQ=255)

                                                         caacaaTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGc                                                                               <  1:9819161/140‑1 (MQ=255)

                                                            caaTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGt                                                                            <  1:340840/140‑1 (MQ=255)

                                                            caaTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGt                                                                            <  1:6026520/140‑1 (MQ=255)

                                                              aTCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCt                                                                          >  1:1090255/1‑140 (MQ=255)

                                                                cGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTgg                                                                        >  1:1293644/1‑140 (MQ=255)

                                                                  ccACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTTAAAGCCTATCAAATCGCCATTGTCATCTCCCTGCTATCGTTTTCTTTTATGGGCGATATTTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTc                                                                      >  2:4461251/1‑140 (MQ=255)

                                                                       gACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGtttt                                                                 >  1:5235732/1‑140 (MQ=255)

                                                                          cgtCAGGCCACGCCAGCCAGTTCCATCTGGGTAGTGACCGCCTATCAAAGCGCCATGGTCATCTCCCTGCTCGCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGCCGTATTTAAAAATGCGGTCTGGTCGTTTTTCt                                                              <  2:6103441/138‑1 (MQ=255)

                                                                          cTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACTTATTTATAAATGCGGTCTGGTCGTTTTTCt                                                              >  2:7762356/1‑140 (MQ=255)

                                                                          cTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCt                                                              <  2:7136207/140‑1 (MQ=255)

                                                                            tCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTTGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgt                                                            <  1:5758934/140‑1 (MQ=255)

                                                                               tGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgttgt                                                         <  2:2420329/140‑1 (MQ=255)

                                                                               tGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgttgt                                                         <  2:6463972/140‑1 (MQ=255)

                                                                               tGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTGTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgttgt                                                         <  2:1438735/140‑1 (MQ=255)

                                                                               tGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATGGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgttgt                                                         <  2:6622718/140‑1 (MQ=255)

                                                                               tGCCACGCCAGCCAGTTCCATCTGGGAAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgttgt                                                         <  2:6259890/140‑1 (MQ=255)

                                                                               agCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCtgttgt                                                         <  1:10561646/139‑1 (MQ=255)

                                                                                 ccACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCt                                                       >  1:2298123/1‑140 (MQ=255)

                                                                                  cACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCtt                                                      >  2:5588523/1‑140 (MQ=255)

                                                                                   aCGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTc                                                     >  1:6636245/1‑140 (MQ=255)

                                                                                    cGCCAGCCAGTTCCTTCTGGGTAGTGAACGCCTATAAATTCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCa                                                    <  2:10240656/140‑1 (MQ=255)

                                                                                      ccagccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTTGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCGTCACt                                                  >  2:10069404/1‑140 (MQ=255)

                                                                                      ccagccagTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACt                                                  <  1:7543641/140‑1 (MQ=255)

                                                                                      ccagccagTTCCATCTGGGTAGAGAACGCCTATCACAGCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACt                                                  <  2:5897338/140‑1 (MQ=255)

                                                                                               tCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTgcgc                                         <  1:1995945/140‑1 (MQ=255)

                                                                                                     tGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTc                                   <  1:6583571/140‑1 (MQ=255)

                                                                                                      gggTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATCTGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTGTCTGTTGTCTTCACGGTGCTGCGCCCTTTCt                                  >  1:7897112/1‑140 (MQ=255)

                                                                                                        gTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTTTGTTAACTGTTCTGAGCCCGTTCTgt                                >  1:2033929/1‑139 (MQ=255)

                                                                                                        gTAGTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGa                                >  2:2643170/1‑140 (MQ=255)

                                                                                                           gTGATCGCCTATCAACTCGCCATTGTCATATCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTACACTGTTCTGCGCCCTTTCTGAtac                             <  2:4876372/140‑3 (MQ=255)

                                                                                                           gTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTc                             <  2:732938/140‑1 (MQ=255)

                                                                                                           gTGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGATTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTc                             <  2:9465932/140‑1 (MQ=255)

                                                                                                            tGAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTTTTTTATTCACATTTCTGCGCCCTTTCTGAtaag                            >  1:1859279/1‑137 (MQ=255)

                                                                                                             gAACGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGc                           >  1:2735542/1‑140 (MQ=255)

                                                                                                              aaCGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCt                                                                          >  1:2901881/1‑92 (MQ=255)

                                                                                                              aaCGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCt                                                                          <  2:2901881/92‑1 (MQ=255)

                                                                                                                cGCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTGTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGc                        <  2:10799024/140‑1 (MQ=255)

                                                                                                                 gCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCa                       <  2:8172084/140‑1 (MQ=255)

                                                                                                                 gCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCa                       >  1:8033101/1‑140 (MQ=255)

                                                                                                                 gCCTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCa                       <  2:6926092/140‑1 (MQ=255)

                                                                                                                   cTATCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCGTTCTGATTCGCTGCaaa                     >  1:8236511/1‑140 (MQ=255)

                                                                                                                      tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATTTGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCGCTTTCTGATTCGCTGCAAATGc                  >  2:5281285/1‑140 (MQ=255)

                                                                                                                      tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAAttc                  >  1:5965397/1‑138 (MQ=255)

                                                                                                                      tCAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGc                  >  2:1177882/1‑140 (MQ=255)

                                                                                                                       cAAATCGCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCt                 <  2:4233287/140‑1 (MQ=255)

                                                                                                                        cactCGCCATTGGCATCTCCCTGCTCTCGAGTTCGTGTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGAGTCGCTGCAAATGCTc                <  1:3555764/137‑1 (MQ=255)

                                                                                                                             gCCATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCt           >  1:5605156/1‑140 (MQ=255)

                                                                                                                               cATTGTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTGGGGTGTGTTCACTGTTCTGCGCCCGTTATGATTCGCTGCAAATGCTCACCCTTg         >  1:4025395/1‑140 (MQ=255)

                                                                                                                                   gTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGcgcg     <  2:2991085/140‑1 (MQ=255)

                                                                                                                                   gTCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGcgcg     <  1:8719672/140‑1 (MQ=255)

                                                                                                                                    tCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCaa                      <  2:4123698/122‑1 (MQ=255)

                                                                                                                                    tCATCTCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTGTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCAGCAAATGCTCACCCTAGCGCgt    >  2:9233327/1‑140 (MQ=255)

TTGTGATTGGTATTTCGATGGCCGTCCTTGACGGCGCAATCGCCAACGTCGCCCTGCCAACAATCGCCACGGACCTTCATGCCACGCCAGCCAGTTCCATCTGGGTAGTGAACGCCTATCAAATCGCCATTGTCATCCCCCTGCTCTCGTTTTCGTTTCTGGGCGATATGTTTGGCTATCGACGTATTTATAAATGCGGTCTGGTCGTTTTTCTGTTGTCTTCACTGTTCTGCGCCCTTTCTGATTCGCTGCAAATGCTCACCCTTGCGCGTGT  >  NZ_CP025268/1915150‑1915423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`