BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,931,596	A→G	221 (TAA→TAG) D686D (GAT→GAC)	exoX → ptrB ←	exodeoxyribonuclease X oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,931,596	0	A	G	99.4%	2214.2 / ‑5.1	638	221 (TAA→TAG) D686D (GAT→GAC)	exoX ptrB	exodeoxyribonuclease X oligopeptidase B
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (266/367); minor base C (0/4); total (266/372)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 638 total aligned reads displayed.

TTTGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAT  >  NZ_CP025268/1931459‑1931728

tttGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTc                                                                                                                                    >  1:7656945/1‑140 (MQ=255)

 ttGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTcc                                                                                                                                   <  1:6642691/140‑1 (MQ=255)

  tGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCg                                                                                                                                  >  1:3980497/1‑140 (MQ=255)

   ggCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGc                                                                                                                                 <  2:4598317/140‑1 (MQ=255)

   ggCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGc                                                                                                                                 >  1:3228687/1‑140 (MQ=255)

   ggCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGc                                                                                                                                 >  2:8900547/1‑140 (MQ=255)

         tACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGt                                                                                                                           <  1:549442/140‑1 (MQ=255)

         tACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGt                                                                                                                           >  1:5028862/1‑140 (MQ=255)

          aCCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTa                                                                                                                          <  1:672125/140‑1 (MQ=255)

           ccGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAg                                                                                                                         <  2:839378/140‑1 (MQ=255)

            cGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGc                                                                                                                        <  2:10098297/140‑1 (MQ=255)

              tGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCgg                                                                                                                      >  1:6412361/1‑140 (MQ=255)

              tGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCgg                                                                                                                      >  1:7216979/1‑140 (MQ=255)

              tGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCgg                                                                                                                      >  2:1590159/1‑140 (MQ=255)

              tGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCgg                                                                                                                      >  2:5669572/1‑140 (MQ=255)

              tGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCgg                                                                                                                      <  2:4963926/140‑1 (MQ=255)

              tGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCgg                                                                                                                      >  2:7574662/1‑140 (MQ=255)

                   aaGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTCTGTCATACCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAGCATTAGCTGGAAAGTACTTAGTCCGCAGGCGGAGCGGGtcag                                                                                                                 >  2:2013070/1‑137 (MQ=255)

                    aGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATg                                                                                                                >  2:2586015/1‑140 (MQ=255)

                        gTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTCATGTTcc                                                                                                            >  1:6002052/1‑140 (MQ=255)

                         tttCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTccc                                                                                                           <  2:5763483/140‑1 (MQ=255)

                         tttCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTccc                                                                                                           <  2:5283649/140‑1 (MQ=255)

                                  ttGCCGAACGCGANCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCNTAGCGGGTAATGTTCCCTGCGCCAgc                                                                                                  >  1:5854591/1‑140 (MQ=255)

                                   tGCCGAAGGCGTTGCGGGGTAGCTGCTCTGGTTATTTAATAACCTGGAGAGCATGAGCACGGAGCTGCGTTTTACAGTGTAACAGTCTCTGGAAATTAGTTAGTCCGCTGGCGTAGCGGGTTATGTTCCCTGCGCCAgcg                                                                                                 >  2:4417030/1‑140 (MQ=255)

                                     ccGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGcgcg                                                                                               >  1:356786/1‑140 (MQ=255)

                                       gAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGAc                                                                                             <  1:9140498/140‑1 (MQ=255)

                                        aaCGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGAcc                                                                                            >  1:9284466/1‑140 (MQ=255)

                                           gcgATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGa                                                                                         <  2:2277620/140‑1 (MQ=255)

                                            cgATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGaa                                                                                        <  1:6897610/140‑1 (MQ=255)

                                               tCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTCATGTTCCCTGCGCCAGCGCGACCAGAAAAg                                                                                     >  2:621494/1‑140 (MQ=255)

                                               tCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAg                                                                                     >  1:8944316/1‑140 (MQ=255)

                                                ccGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGc                                                                                    <  2:8485575/140‑1 (MQ=255)

                                                 cGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCa                                                                                   <  2:1044738/140‑1 (MQ=255)

                                                       aTCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                             <  2:9571617/140‑1 (MQ=255)

                                                       aTCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACAGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTcc                                                                             <  2:3300301/140‑1 (MQ=255)

                                                         cTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCAt                                                                           >  1:4714636/1‑140 (MQ=255)

                                                         cTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCAt                                                                           >  1:8123539/1‑140 (MQ=255)

                                                            cgcTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGc                                                                        >  1:2475756/1‑140 (MQ=255)

                                                             gcTGGTTATTTTATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCt                                                                       >  1:2659419/1‑140 (MQ=255)

                                                             gcTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCt                                                                       >  1:426299/1‑140 (MQ=255)

                                                             gcTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCt                                                                       >  1:9570094/1‑140 (MQ=255)

                                                              cTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATACCATCGCTa                                                                      >  2:6414193/1‑140 (MQ=255)

                                                                  ttATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGcc                                                                  >  1:5677518/1‑140 (MQ=255)

                                                                  ttATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGcc                                                                  >  1:1542150/1‑140 (MQ=255)

                                                                  ttATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGcc                                                                  >  2:8132952/1‑140 (MQ=255)

                                                                  ttATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGcc                                                                  >  2:7025711/1‑140 (MQ=255)

                                                                       taatcaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTCTCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGt                                                             <  1:1060722/140‑1 (MQ=255)

                                                                       taataaCCTGGACAGCATGAGCCCGGAGCTGCGTTTACCACTGAAACATGATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGGTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGt                                                             <  2:10803144/140‑1 (MQ=255)

                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGa                                                         >  2:2712145/1‑140 (MQ=255)

                                                                           aaCCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGa                                                         >  1:3808065/1‑140 (MQ=255)

                                                                               tGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTa                                                     >  2:680029/1‑140 (MQ=255)

                                                                                ggACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTaa                                                    >  1:7803349/1‑140 (MQ=255)

                                                                                 gACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGATACGCATTCGTACGATTTaaa                                                   <  2:7169403/140‑1 (MQ=255)

                                                                                      cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGAc                                              >  2:5123337/1‑140 (MQ=255)

                                                                                      cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGAc                                              >  1:4477050/1‑140 (MQ=255)

                                                                                      cATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGAc                                              >  1:2194744/1‑140 (MQ=255)

                                                                                       aTGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGAcc                                             <  2:10551933/140‑1 (MQ=255)

                                                                                       aTGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGAcc                                             <  1:1748514/140‑1 (MQ=255)

                                                                                       aTGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGAcc                                             <  2:2828257/140‑1 (MQ=255)

                                                                                       aTGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCAGCTTCCATCGCTACGCCTTCGTACGATGTAAAGCGAcc                                             >  2:10204227/1‑140 (MQ=255)

                                                                                       aTGAGCCCGGAGCTGCGTTTAAAACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGAcc                                             <  1:523092/140‑1 (MQ=255)

                                                                                        tGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTCCGCCTTCGTACGATTTAAAGCGACCa                                            <  1:9758231/140‑1 (MQ=255)

                                                                                        tGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTCCGCCTTCGTACGATTTAAAGCGACCa                                            >  2:8672608/1‑140 (MQ=255)

                                                                                           gCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGAt                                         >  1:1112114/1‑140 (MQ=255)

                                                                                              cGGAGCTGCGTTTAACACTGAAACATTCTCTGGAAAATACTCAGTCCGCCGGCGAAGCGGGTAATGTTCCCTGCGCCCGCGCGACCAGAAAAGCATATTCCATCGCTACCCCTTCGTACGATTTAACGCGACCAGATTTa                                      <  1:1249848/140‑1 (MQ=255)

                                                                                              cGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTa                                      <  2:6065816/140‑1 (MQ=255)

                                                                                                gAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTAcc                                    >  1:10009275/1‑140 (MQ=255)

                                                                                                   cTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGcc                                 <  1:581414/140‑1 (MQ=255)

                                                                                                    tGCGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCa                                <  1:7401775/140‑1 (MQ=255)

                                                                                                      cGTTTAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATg                              >  2:1381724/1‑140 (MQ=255)

                                                                                                        tttAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGc                            <  1:8040810/140‑1 (MQ=255)

                                                                                                          tAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGTGTATTGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGTTTTAAAGCGACCAGATTTACCGCCATGGCCt                          <  2:7886133/140‑1 (MQ=255)

                                                                                                          tAACACTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCt                          <  1:10810687/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTCGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACTATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  1:1028969/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  1:4199655/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  2:3430885/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  1:9937659/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  2:632429/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  1:8685493/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  1:3103987/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTACGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  1:412662/140‑1 (MQ=255)

                                                                                                               cTGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCAATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  1:2744577/140‑1 (MQ=255)

                                                                                                               atGAAACATTATCTGTAAAATACTTAGTCCGCTGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTc                     <  1:8449937/139‑1 (MQ=255)

                                                                                                                tGAAACATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTcc                    >  2:370935/1‑140 (MQ=255)

                                                                                                                  aaaCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTa                                                            >  2:28697/1‑98 (MQ=255)

                                                                                                                  aaaCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCAt                  >  1:2271360/1‑140 (MQ=255)

                                                                                                                   aaCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTa                                                                      <  1:6218010/87‑1 (MQ=255)

                                                                                                                    aCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCGTATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGt                <  2:9223753/140‑1 (MQ=255)

                                                                                                                    aCATTATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGt                <  2:1218044/140‑1 (MQ=255)

                                                                                                                        tATCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGt            >  2:3909330/1‑140 (MQ=255)

                                                                                                                         aTCTGGAAAATACTTCGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCAGGGCCTGAGTCCAGGTCGGTa           <  2:4798324/140‑1 (MQ=255)

                                                                                                                         aTCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa           <  1:4616628/140‑1 (MQ=255)

                                                                                                                         aTCTGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTa           <  2:426299/140‑1 (MQ=255)

                                                                                                                            tGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACag        <  1:5528/140‑1 (MQ=255)

                                                                                                                            tGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACag        <  2:1817906/140‑1 (MQ=255)

                                                                                                                            tGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACag        <  1:2025471/140‑1 (MQ=255)

                                                                                                                            tGGAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATGTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTCCag        >  2:9043482/1‑140 (MQ=255)

                                                                                                                             ggAAAATACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACaga       <  2:5474778/140‑1 (MQ=255)

                                                                                                                              caaaaTACCTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACagag      <  1:7005996/139‑1 (MQ=255)

                                                                                                                                aaaTACTTAGTCCGCAGGCGTAGCGGGTAATGTTCCCTTCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCa    <  2:9788156/140‑1 (MQ=255)

TTTGGCAAATACCGTGGCAAAGCGGTTTCCGACGTTGCCGAACGCGATCCGGGCTATCTGCGCTGGTTATTTAATAACCTGGACAGCATGAGCCCGGAGCTGCGTTTAACACTGAAACATTATCTGGAAAATACTTAATCCGCAGGCGTAGCGGGTAATGTTCCCTGCGCCAGCGCGACCAGAAAAGCATATTCCATCGCTACGCCTTCGTACGATTTAAAGCGACCAGATTTACCGCCATGGCCTGAGTCCATGTCGGTACAGAGCAAT  >  NZ_CP025268/1931459‑1931728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`