BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,002,534	T→C	329 (TAA→TAG)	dcyD ←	D‑cysteine desulfhydrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,002,534	0	T	C	99.7%	2335.1 / NA	671	329 (TAA→TAG)	dcyD	D‑cysteine desulfhydrase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (313/355); minor base A (2/0); total (316/355)
Fisher's exact test for biased strand distribution p-value = 2.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 671 total aligned reads displayed.

CGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT  >  NZ_CP025268/2002397‑2002670

cgcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTa                                                                                                                                        >  1:10897031/1‑140 (MQ=255)

cgcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGGTGTCTACTTTCTTGCATGAGCGTTTCCGGAGCTa                                                                                                                                        >  2:9485852/1‑140 (MQ=255)

cgcgAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAAGCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCAGGAGCTa                                                                                                                                        >  2:9824988/1‑140 (MQ=255)

   gagaATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAAc                                                                                                                                     <  2:4939280/140‑1 (MQ=255)

      aaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg                                                                                                                                  <  2:5030152/140‑1 (MQ=255)

      aaTAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg                                                                                                                                  <  2:3598022/140‑1 (MQ=255)

         aaaCCCGATCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAATGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgggg                                                                                                                               <  2:10201890/140‑1 (MQ=255)

         aaaCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTgggg                                                                                                                               <  1:4163753/140‑1 (MQ=255)

           aCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat                                                                                                                             <  2:4669626/140‑1 (MQ=255)

           aCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGgat                                                                                                                             <  1:4276266/140‑1 (MQ=255)

           aCCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTAGACCCAGCCCCTTTCAGCAGGAACGGCATGGAATCAATATCCCGTGGTATACTGTCGTGAATGAGCGTTTCCGGAGCTAAACGTGGGgat                                                                                                                             <  2:6591595/140‑1 (MQ=255)

                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGCATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAg                                                                                                                        <  1:8214665/140‑1 (MQ=255)

                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAg                                                                                                                        >  1:5135574/1‑140 (MQ=255)

                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAg                                                                                                                        >  1:6764538/1‑140 (MQ=255)

                aGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAg                                                                                                                        <  1:3362086/140‑1 (MQ=255)

                   aGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCg                                                                                                                     <  2:4038790/140‑1 (MQ=255)

                   aGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCg                                                                                                                     <  2:4853429/140‑1 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                                   <  1:1911076/140‑1 (MQ=255)

                     tAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGaa                                                                                                                   <  1:1758205/140‑1 (MQ=255)

                      aaGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAAc                                                                                                                  <  1:1603346/140‑1 (MQ=255)

                       aGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACa                                                                                                                 >  1:2677245/1‑140 (MQ=255)

                               aaCATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGGGGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGgc                                                                                                         >  2:7176635/1‑140 (MQ=255)

                                     ccgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                                   <  2:6914179/140‑1 (MQ=255)

                                     ccgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccg                                                                                                   <  1:5937483/140‑1 (MQ=255)

                                      cgccgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGGTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCAccgc                                                                                                  <  2:4571768/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCTTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  2:1470955/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  2:10887444/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  2:3010400/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  2:836310/140‑1 (MQ=255)

                                         cgATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  1:3873044/140‑1 (MQ=255)

                                         cgATACTGAGTAGCACCCTATACCCAGCCCCATTCAGCAGGAACGGCAAGGAATCAATAACCACTTGTATACTATCTTGCAAGAGCCTTTCCGGAGCTAAACGTGCGGATGATAGGCGAACAGCGCAGGCGCACCGCCgg                                                                                               <  2:7457489/140‑1 (MQ=255)

                                           aTACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTa                                                                                             <  1:10794811/140‑1 (MQ=255)

                                           aTACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGGTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTa                                                                                             <  2:2369672/140‑1 (MQ=255)

                                              cTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGa                                                                                          <  2:2125267/140‑1 (MQ=255)

                                              cTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCGAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGa                                                                                          <  2:560760/140‑1 (MQ=255)

                                               tGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGaa                                                                                         <  1:9733760/140‑1 (MQ=255)

                                                  gtggCAGCGGAGACCCAGCCCCTGTCAGCAGGAACGGCCAGGAAGCACTCACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATaa                                                                                      <  2:2686632/137‑1 (MQ=255)

                                                  gTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCTAACAGCGCAGGCGCACCGCCGGTATGAATaa                                                                                      >  2:5246229/1‑140 (MQ=255)

                                                  gTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATaa                                                                                      >  2:2778784/1‑140 (MQ=255)

                                                   ttGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATaaa                                                                                     <  2:1238613/140‑1 (MQ=255)

                                                   ttGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATaaa                                                                                     <  1:8385612/140‑1 (MQ=255)

                                                     gCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACa                                                                                   >  1:3994887/1‑140 (MQ=255)

                                                      cAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAg                                                                                  >  2:8022618/1‑140 (MQ=255)

                                                      cAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAg                                                                                  >  1:553118/1‑140 (MQ=255)

                                                         cGTATACCCAGCCCAGATCAGCAGCTACGGCAACCACACCATCACCAGTTGTATACAAAACAGCATGAGCGTATCCGGAGCGAAACCTTGGTATGATAGGCGCACAGCGCTGGCGCACCGCCGGTATGAATAAAccgcct                                                                               <  2:7795766/140‑6 (MQ=255)

                                                           tataCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCg                                                                             >  1:2616208/1‑140 (MQ=255)

                                                            ataCCCAGCCCCTATCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCgg                                                                            <  2:10584300/140‑1 (MQ=255)

                                                             taCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGc                                                                           >  1:4706767/1‑140 (MQ=255)

                                                             taCCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGc                                                                           >  1:6231330/1‑140 (MQ=255)

                                                                 cAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCtt                                                                       <  1:561833/140‑1 (MQ=255)

                                                                     cccTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTg                                                                                                                                  >  1:4890415/1‑77 (MQ=255)

                                                                       cTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAAt                                                                               <  1:229755/126‑1 (MQ=255)

                                                                         ttCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTg                                                               >  2:1126754/1‑140 (MQ=255)

                                                                           cagcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGaa                                                             <  1:5365164/140‑1 (MQ=255)

                                                                           cagcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGaa                                                             <  1:2943014/140‑1 (MQ=255)

                                                                           cagcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGaa                                                             <  1:7148894/140‑1 (MQ=255)

                                                                             gcagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGc                                                           <  1:2180391/140‑1 (MQ=255)

                                                                              cagGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCg                                                          >  1:3534178/1‑140 (MQ=255)

                                                                                         aGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTctgactga                                               <  2:9332861/140‑1 (MQ=255)

                                                                                          ggAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGAt                                              <  2:2616208/140‑1 (MQ=255)

                                                                                           gAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATa                                             >  2:4199268/1‑140 (MQ=255)

                                                                                           gAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATa                                             >  2:10006420/1‑140 (MQ=255)

                                                                                           gAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATa                                             >  1:4456215/1‑140 (MQ=255)

                                                                                               cAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGACTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGt                                         <  1:2855602/140‑1 (MQ=255)

                                                                                               cAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGt                                         <  1:8566562/140‑1 (MQ=255)

                                                                                                 aTAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCa                                       >  1:759917/1‑140 (MQ=255)

                                                                                                      cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCCATCAg                                  >  2:5879711/1‑140 (MQ=255)

                                                                                                      cAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAg                                  >  1:10478698/1‑140 (MQ=255)

                                                                                                        gTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                                >  2:3800965/1‑140 (MQ=255)

                                                                                                        gTTGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGcc                                >  1:1502048/1‑140 (MQ=255)

                                                                                                         ttGTATACTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGccc                               >  2:8210062/1‑140 (MQ=255)

                                                                                                            tataCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGc                            >  2:5761260/1‑140 (MQ=255)

                                                                                                             ataCTTTCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGcc                           <  1:10015600/140‑1 (MQ=255)

                                                                                                                 tttCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCg                       >  1:7336956/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGCTAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCCTCGCt                     >  1:10655251/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  1:10235236/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  2:6057873/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  1:652834/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  1:7022517/1‑140 (MQ=255)

                                                                                                                   tCTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTGTCTGACTGATACCGTCAATCAGCCCCGCCATCGCt                     >  2:8819809/1‑140 (MQ=255)

                                                                                                                    cTTGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCCTCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCta                    <  2:1962017/140‑2 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGTAGCTAAACGTGGGGATGATAGGCGAACATCGCAGGCGCACCTCCGGTATTAATAAAAATAATCGTCCCTTAATATTTGAAGCGGTTCTGACTGATAACGTCAATCATCACCGCCATCGCttat                  >  2:3250859/1‑138 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  <  1:9055433/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  <  2:9737006/140‑1 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  >  2:7114777/1‑140 (MQ=255)

                                                                                                                      tGCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCtttt                  <  1:4598279/140‑1 (MQ=255)

                                                                                                                       gCATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTc                 <  1:9587179/140‑1 (MQ=255)

                                                                                                                        cATGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTcc                >  2:8986827/1‑140 (MQ=255)

                                                                                                                         aTGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCg               <  2:1684130/140‑1 (MQ=255)

                                                                                                                          tGAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCgg              >  1:5787268/1‑140 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACTGAATCGGCCCTTCATCTTTGAAGCGTTTCTGTCTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  1:4969853/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             >  1:1291736/1‑140 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  1:2412702/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  1:7723814/140‑1 (MQ=255)

                                                                                                                           gAGCGTTTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  1:8979358/140‑1 (MQ=255)

                                                                                                                           gAGCGTGTCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGt             <  2:6849499/140‑1 (MQ=255)

                                                                                                                                tttCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATaca        <  2:6231330/140‑1 (MQ=255)

                                                                                                                                  tCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGACTCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca      <  2:7611714/140‑1 (MQ=255)

                                                                                                                                  tCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca      >  1:3114249/1‑140 (MQ=255)

                                                                                                                                  tCCGGAGCTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATAcaca      >  1:209266/1‑140 (MQ=255)

CGCGAGAATAAACCCGAGCAGTAAGCCAAAAAACATGCCGCCGATACTGAGTTGCAGCGTATACCCAGCCCCTTTCAGCAGGAACGGCAAGGAATCAATAACCAGTTGTATACTTTCTTGCATGAGCGTTTCCGGAGTTAAACGTGGGGATGATAGGCGAACAGCGCAGGCGCACCGCCGGTATGAATAAACAGAATCGGCCCTTCATCTTTGAAGCGTTTCTGACTGATACCGTCAATCAGCCCCGCCATCGCTTTTCCGGTATACACAGGAT  >  NZ_CP025268/2002397‑2002670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`