BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,846,593	T→C	157 (TAA→TAG)	hycI ←	hydrogenase maturation peptidase HycI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,846,593	0	T	C	99.7%	2179.0 / NA	626	157 (TAA→TAG)	hycI	hydrogenase maturation peptidase HycI
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (359/265); minor base G (1/1); total (360/266)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 628 total aligned reads displayed.

GAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGC  >  NZ_CP025268/2846457‑2846729

gAACGCCTTATCCGGCCTACCAGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTAc                                                                                                                                       <  2:7435475/140‑1 (MQ=255)

gAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTAc                                                                                                                                       <  1:10015736/140‑1 (MQ=255)

gAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTAc                                                                                                                                       <  1:5885941/140‑1 (MQ=255)

gAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGAGAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTAc                                                                                                                                       <  1:5446382/140‑1 (MQ=255)

       ttATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCATTTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTcttctt                                                                                                                                <  1:2821831/140‑1 (MQ=255)

       ttATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTcttctt                                                                                                                                <  1:10140764/140‑1 (MQ=255)

       ttATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTcttctt                                                                                                                                >  1:6605111/1‑140 (MQ=255)

       ttATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTcttctt                                                                                                                                <  2:3845412/140‑1 (MQ=255)

       ttATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTcttctt                                                                                                                                <  2:6579115/140‑1 (MQ=255)

       ttATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCAACGGGCAATTTAGCATTTGGCATCAGAGCTGTTTCACCCCATCAAGAACATCCATGTCCTGATTCCTTAATGAAAAACTACTcttctt                                                                                                                                <  1:3509378/140‑1 (MQ=255)

        tATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCttcttc                                                                                                                               <  1:8471212/140‑1 (MQ=255)

         aTCCGGCCTACCTGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              <  2:8140675/140‑1 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              >  1:8094260/1‑140 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              <  2:9447864/140‑1 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              >  2:477240/1‑140 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              <  1:4843128/140‑1 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              >  1:7236747/1‑140 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              <  2:6378508/140‑1 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              >  1:1640391/1‑140 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              <  1:2437216/140‑1 (MQ=255)

         aTCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTcc                                                                                                                              <  1:7648209/140‑1 (MQ=255)

              gCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGc                                                                                                                         >  2:5848198/1‑140 (MQ=255)

               ccTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCGGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCt                                                                                                                        >  1:1400516/1‑140 (MQ=255)

                cTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTa                                                                                                                       >  1:3103503/1‑140 (MQ=255)

                cTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTa                                                                                                                       >  1:2172965/1‑140 (MQ=255)

                 aaCATGTCGATCTATCCAGGAAGGCCAGATAAGGGCTGCGCATCGGGCTATTTATCATTTGGCAGCAGAGCTGGTTCTCCCCATAAAGAAAATCCAGGTCCTGATTCCTTAATGAAAAACTACTCTTCTTACACCGCTaa                                                                                                                      <  2:9209113/139‑1 (MQ=255)

                        cGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGcgcg                                                                                                               <  1:1308639/140‑1 (MQ=255)

                         gCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTAAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGa                                                                                                              >  1:7021234/1‑140 (MQ=255)

                         gCACGAACCATGAAGGCCCGAAAAGAGCAGCGCAACGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATAAAGAACATCACTGTCCATATTCCTTAATGAAAAACTACTATTCTTCCACCTCTAACTGCGCGa                                                                                                              >  1:6427705/1‑140 (MQ=255)

                          cACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTACTTCCACCGCTAACTGCGCGaa                                                                                                             >  2:7346287/1‑140 (MQ=255)

                             gAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAgcc                                                                                                          >  1:6772270/1‑140 (MQ=255)

                              aaCCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAgccg                                                                                                         >  2:7829387/1‑140 (MQ=255)

                                ccATGACGGCCCGAGCAGCGCAGCGCATCGGGCAATTGAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACGACTCTTCTTCCACCGCTAACTGCGCGAAgccgcc                                                                                                       <  2:8711734/140‑1 (MQ=255)

                                ccATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCCTTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAgccgcc                                                                                                       <  2:10746871/140‑1 (MQ=255)

                                         cccGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATttccttc                                                                                              >  2:8299166/1‑140 (MQ=255)

                                          ccGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATttccttcc                                                                                             <  2:5659055/140‑1 (MQ=255)

                                           cGATAAGCGCAGCGCATCGGGCCCTTTAGCCTGAGGCCTCAGAGCTGGTTCACCCCATCAAGAACAGCCCGGTCCTGCGTCCTTAATGAAACACTACGCTTCTTCCCCCGCTAACTGCGCGAAGCCGCCATTTCCTTccc                                                                                            >  2:556953/1‑140 (MQ=255)

                                           cGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTccc                                                                                            >  1:4637096/1‑140 (MQ=255)

                                                  cgcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                     <  1:1201447/140‑1 (MQ=255)

                                                  cgcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTc                                                                                     >  2:8991289/1‑140 (MQ=255)

                                                   gcagcgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTcc                                                                                    >  1:8847811/1‑140 (MQ=255)

                                                      gcgcaTCGGGCAATTTAGCATGTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGt                                                                                 <  2:6009001/140‑1 (MQ=255)

                                                       cgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCCTCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAggc                                                                                >  2:9684835/1‑138 (MQ=255)

                                                       cgcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTc                                                                                >  2:10157606/1‑140 (MQ=255)

                                                        gcaTCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCg                                                                               <  2:4201414/140‑1 (MQ=255)

                                                               gCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATaa                                                                        <  1:4179887/140‑1 (MQ=255)

                                                                 aaTTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAAc                                                                      >  1:3749315/1‑140 (MQ=255)

                                                                   tttAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCGAGTCGTTGATAAACgg                                                                    >  2:2244558/1‑140 (MQ=255)

                                                                   tttAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACgg                                                                    >  1:4330077/1‑140 (MQ=255)

                                                                        cATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCGTCTTCCACCGCTACCTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGAGAAACGGTGTCt                                                               >  2:7620032/1‑140 (MQ=255)

                                                                             ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGc                                                          >  1:10339302/1‑140 (MQ=255)

                                                                             ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGc                                                          <  1:10575712/140‑1 (MQ=255)

                                                                             ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGc                                                          >  2:10435868/1‑140 (MQ=255)

                                                                             ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGGTGATCAACGGTTTCTACCGc                                                          >  2:9587602/1‑140 (MQ=255)

                                                                             ggCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTAAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTAGTTGATAAACTGTTTCTACCGc                                                          >  1:8351282/1‑140 (MQ=255)

                                                                             ggCATCAGAGCTGGGTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTACCACCGCTAACTGCGCGAAGCCGCCATTTCATTCCCAGCCTACCAGTCGTTGAAAAACCGTTTCTACAGc                                                          >  1:1799466/1‑140 (MQ=255)

                                                                               cATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCAt                                                        <  1:6307047/140‑1 (MQ=255)

                                                                                     agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTaa                                                  >  2:100222/1‑140 (MQ=255)

                                                                                     agCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTaa                                                  >  1:9551329/1‑140 (MQ=255)

                                                                                       cTGGTTCACCCCATCAAGAACAACCCGGTCCTGAGTCCATAATGAAAAACGACTCTACTTCCAACGCTATCTTCGCGAAGCCGCCATTTCCTGCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATc                                                <  2:7074230/140‑1 (MQ=255)

                                                                                        tGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCg                                               >  2:1300760/1‑140 (MQ=255)

                                                                                               ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGt                                        >  2:10534112/1‑140 (MQ=255)

                                                                                               ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGt                                        >  1:10722219/1‑140 (MQ=255)

                                                                                               ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGt                                        >  2:1861470/1‑140 (MQ=255)

                                                                                               ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGt                                        >  1:9686715/1‑140 (MQ=255)

                                                                                               ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGt                                        >  2:2773099/1‑140 (MQ=255)

                                                                                               ccccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCAGCCATTTCCTTCGCAGCCTTCAAGTCGTGGATCAACGGTGTCTACCGCATCTTTATTCGGCTgggg                                        >  2:6934012/1‑139 (MQ=255)

                                                                                                 ccATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCa                                      <  1:7820878/140‑1 (MQ=255)

                                                                                                  cATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCAt                                     <  2:305830/140‑1 (MQ=255)

                                                                                                    tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAactact                                                                                                                                      <  2:3954403/41‑1 (MQ=255)

                                                                                                    tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCg                                   <  2:10385188/140‑1 (MQ=255)

                                                                                                    tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCg                                   >  2:36376/1‑140 (MQ=255)

                                                                                                    tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCg                                   <  2:3135216/140‑1 (MQ=255)

                                                                                                    tCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCg                                   >  1:3689360/1‑140 (MQ=255)

                                                                                                     cAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCgg                                  <  2:1652184/140‑1 (MQ=255)

                                                                                                     cAAGAACATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCgg                                  >  1:2034948/1‑140 (MQ=255)

                                                                                                         aaCATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgtag                              >  1:4452163/1‑140 (MQ=255)

                                                                                                          aCATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgtagt                             <  2:5138231/140‑1 (MQ=255)

                                                                                                          aCATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgtagt                             >  2:432024/1‑140 (MQ=255)

                                                                                                          aCATCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATGTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgtagt                             >  1:2581188/1‑140 (MQ=255)

                                                                                                           cctCCCTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGgtagta                            <  2:8042367/138‑1 (MQ=255)

                                                                                                              cccTGTCCTGATTCCTTAATGAACAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCAGTTCCGTCCCAGCCTTCCAGTAGTTGAGAAACGGTGTCTACCGCCTCTGTCATCGGCTGGGTCATCGGGGAGGaaaa                         >  2:1460493/1‑140 (MQ=255)

                                                                                                              cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTaaaa                         >  1:5045712/1‑140 (MQ=255)

                                                                                                              cccTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTaaaa                         >  1:6212562/1‑140 (MQ=255)

                                                                                                              cccTGACCTGATACCTTAATGAAAAACTACTCTGCTTCCACCGCTAACTGCGCGAAGCCGCCATTACCTTCCAAGCCTTCCCGTCGTTGATAAACGGTTTCTACCGCATCTTAAAACGGATGGGTCATCGGGGAGTaaaa                         >  2:3267516/1‑140 (MQ=255)

                                                                                                                cTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGc                       >  1:3839059/1‑140 (MQ=255)

                                                                                                                cTGTCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGc                       >  1:5683550/1‑140 (MQ=255)

                                                                                                                   tCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCcga                    >  1:5133156/1‑140 (MQ=255)

                                                                                                                   tCCTGATTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCcga                    >  2:3449400/1‑140 (MQ=255)

                                                                                                                        aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGata               >  1:6904920/1‑140 (MQ=255)

                                                                                                                        aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGata               >  1:3414993/1‑140 (MQ=255)

                                                                                                                        aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGata               >  1:9334180/1‑140 (MQ=255)

                                                                                                                        aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTGTAATCGGCTGGGTCAGCGGGTCGTAAAAGCCGACGata               >  2:2554793/1‑140 (MQ=255)

                                                                                                                        aTTCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCAAGCCTTCCAGTCGTTGATACACGGTTTCTACCGCATCTTTAATCGGCAGGGTCATCGGGTAGTAAAAGCCGACGata               >  2:525422/1‑140 (MQ=255)

                                                                                                                        aTTCCTTAATGAAAAACTACTCATCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTTATAAACGGTTTCAACCCCATCTTTACTCGGCTGGGTCATCGGGTAGTAAAAGCCGACGata               >  2:5000616/1‑140 (MQ=255)

                                                                                                                          tCCTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATc             <  1:4024682/140‑1 (MQ=255)

                                                                                                                           ccTTAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATcc            >  1:1876130/1‑140 (MQ=255)

                                                                                                                             ttAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCgg          >  1:9000223/1‑140 (MQ=255)

                                                                                                                             ttAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCgg          >  2:8441055/1‑140 (MQ=255)

                                                                                                                             ttAATGAAAAACTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCgg          <  2:4508176/140‑1 (MQ=255)

GAACGCCTTATCCGGCCTACATGTCGCACGAACCATGAAGGCCCGATAAGCGCAGCGCATCGGGCAATTTAGCATTTGGCATCAGAGCTGGTTCACCCCATCAAGAACATCCCTGTCCTGATTCCTTAATGAAAAATTACTCTTCTTCCACCGCTAACTGCGCGAAGCCGCCATTTCCTTCCCAGCCTTCCAGTCGTTGATAAACGGTTTCTACCGCATCTTTAATCGGCTGGGTCATCGGGTAGTAAAAGCCGACGATATCCGGCTGAATGC  >  NZ_CP025268/2846457‑2846729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`