BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	2,960,614	C→T	A192A (GCG→GCA)	ptrA ←	pitrilysin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	2,960,614	0	C	T	99.7%	2288.4 / NA	599	A192A (GCG→GCA)	ptrA	pitrilysin
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (297/300); minor base A (2/0); total (299/300)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 614 total aligned reads displayed.

TTACTGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAT  >  NZ_CP025268/2960477‑2960750

ttaCTGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTgtgc                                                                                                                                        <  1:2440980/140‑1 (MQ=255)

   cTGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCgg                                                                                                                                     >  2:496841/1‑140 (MQ=255)

    tGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCggg                                                                                                                                    >  2:5382725/1‑140 (MQ=255)

     gTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGt                                                                                                                                   >  1:1731575/1‑140 (MQ=255)

      tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:10791565/1‑140 (MQ=255)

      tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  2:1664838/1‑140 (MQ=255)

      tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:2845223/1‑140 (MQ=255)

      tAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGtt                                                                                                                                  >  1:7567473/1‑140 (MQ=255)

       aaaTAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTa                                                                                                                                 <  2:2707293/140‑1 (MQ=255)

             ccGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGtt                                                                                                                           <  1:10636647/140‑1 (MQ=255)

             ccGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGtt                                                                                                                           >  1:3690293/1‑140 (MQ=255)

             ccGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACATTGAACCGGGGTGTGCCGGGTTAATGGtt                                                                                                                           <  1:739073/140‑1 (MQ=255)

             ccGCCTTCATCAAATTGGCGGAATAGTACTTCTAGTGGAAATCTTTCAGCGCCTGCTGCACCGTATTACCAGGTTTGTCGCTTAAATTTTCGAGGTTACCACCAGAAAAATTTGAACCGGGGTGTGCCGGGTTAATGGtt                                                                                                                           >  2:3960391/1‑140 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:5599296/140‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:4874942/140‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:6883396/140‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:2681172/140‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          >  1:929137/1‑140 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  1:9764070/140‑1 (MQ=255)

              cGCCTTCATCAAATTGGCGGAATAGTACTACTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGGCGCTAAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGtttt                                                                                                                          <  2:7438221/140‑1 (MQ=255)

              cGCCTTCATCAAAGTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGttt                                                                                                                          <  2:3044723/140‑1 (MQ=255)

                ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCCGAAAACTTTGAACCGGGGTGTGCCGGTTTAATGGTTTCt                                                                                                                        >  2:8558072/1‑140 (MQ=255)

                ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        >  2:2845008/1‑140 (MQ=255)

                ccTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCt                                                                                                                        <  2:1513314/140‑1 (MQ=255)

                  ttCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgc                                                                                                                      >  2:2568406/1‑140 (MQ=255)

                  ttCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgc                                                                                                                      >  1:5411784/1‑140 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:8250037/140‑1 (MQ=255)

                   tcatcaAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTgcg                                                                                                                     <  1:10023013/140‑1 (MQ=255)

                        aaaTTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGCGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGAc                                                                                                                <  1:6737356/140‑1 (MQ=255)

                           ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGTACCGGGGTGTGCCGGGTTCATGGTTTCTGCGCTGAGCtg                                                                                                             >  1:9589845/1‑140 (MQ=255)

                           ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGTCCtg                                                                                                             >  2:7852766/1‑140 (MQ=255)

                           ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCtg                                                                                                             >  1:1179800/1‑140 (MQ=255)

                           ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCtg                                                                                                             >  1:8521551/1‑140 (MQ=255)

                           ttGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCtg                                                                                                             >  1:3387780/1‑140 (MQ=255)

                                    tAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATgcg                                                                                                    >  2:3289648/1‑140 (MQ=255)

                                     aGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATgcgc                                                                                                   <  1:8616755/140‑1 (MQ=255)

                                           tctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCg                                                                                             <  2:4158398/140‑1 (MQ=255)

                                            ctcGTGGAAATCTTTCGGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCgg                                                                                            >  2:8848648/1‑139 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCTGGTTTGTCGCTTATAGTTTCGAGGTTACCACCAGACAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGTCCGGGGCCATGCGCTTCGCGt                                                                                            >  2:4820507/1‑140 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAATGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  2:3788813/1‑140 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            <  1:311623/140‑1 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  1:8060989/1‑140 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            >  2:9531329/1‑140 (MQ=255)

                                            ctcGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGt                                                                                            <  2:2009492/140‑1 (MQ=255)

                                              cGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCa                                                                                          >  1:8738588/1‑140 (MQ=255)

                                                 ggAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCAcgc                                                                                       >  2:8710239/1‑140 (MQ=255)

                                                 ggAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCAcgc                                                                                       >  1:5995113/1‑140 (MQ=255)

                                                 ggAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCAcgc                                                                                       >  2:10009123/1‑140 (MQ=255)

                                                    aaTCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                                    <  1:3971289/140‑1 (MQ=255)

                                                    aaTCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                                    <  2:9792334/140‑1 (MQ=255)

                                                    aaTCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTa                                                                                    <  2:10314720/140‑1 (MQ=255)

                                                       cTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTAcgc                                                                                 >  2:9329602/1‑140 (MQ=255)

                                                        tttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTAcgcg                                                                                <  2:9906618/140‑1 (MQ=255)

                                                        tttCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTAcgcg                                                                                <  2:7595155/140‑1 (MQ=255)

                                                          tCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGcc                                                                              <  2:2482704/140‑1 (MQ=255)

                                                               gcCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGt                                                                         <  1:4082128/140‑1 (MQ=255)

                                                                ccTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGtt                                                                        >  2:10615571/1‑140 (MQ=255)

                                                                 ctgctgCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTa                                                                       <  1:8905861/140‑1 (MQ=255)

                                                                 ctgctgCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGGTa                                                                       >  2:9209538/1‑140 (MQ=255)

                                                                   gctgcACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAAt                                                                     <  1:2207949/140‑1 (MQ=255)

                                                                    ctgcACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAAtt                                                                    >  1:5769160/1‑140 (MQ=255)

                                                                      gcACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCa                                                                  <  1:1350293/140‑1 (MQ=255)

                                                                        aCCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTCCGCGCCATGGTTAATTCAGc                                                                >  2:1388971/1‑140 (MQ=255)

                                                                         ccGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCg                                                               >  1:5215800/1‑140 (MQ=255)

                                                                          cGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAACACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGt                                                              <  2:8315499/140‑1 (MQ=255)

                                                                          cGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCTCCATGGTTAATTCAGCGt                                                              <  2:5165389/140‑1 (MQ=255)

                                                                           ggATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGtt                                                             >  1:1471232/1‑140 (MQ=255)

                                                                              ttACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCAc                                                          <  1:9469667/140‑1 (MQ=255)

                                                                              ttACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCAc                                                          >  2:653351/1‑140 (MQ=255)

                                                                                   aGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGACAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCAt                                                     <  1:500823/140‑1 (MQ=255)

                                                                                    ggTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGa                                                                                                                 >  1:634374/1‑79 (MQ=255)

                                                                                      tttGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTAc                                                  >  2:8106615/1‑140 (MQ=255)

                                                                                      tttGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCATCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTAc                                                  >  1:3778064/1‑140 (MQ=255)

                                                                                         gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                               <  1:3556130/140‑1 (MQ=255)

                                                                                         gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                               <  1:2001489/140‑1 (MQ=255)

                                                                                         gTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCt                                               >  1:10260344/1‑140 (MQ=255)

                                                                                            gCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCAc                                            <  1:8337210/140‑1 (MQ=255)

                                                                                               tAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGtt                                         >  2:10652013/1‑140 (MQ=255)

                                                                                               tAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGtt                                         <  2:3533746/140‑1 (MQ=255)

                                                                                               tAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGtt                                         <  1:5129228/140‑1 (MQ=255)

                                                                                                   gTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGc                                     <  1:4463917/140‑1 (MQ=255)

                                                                                                   gTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGc                                     <  2:2128827/140‑1 (MQ=255)

                                                                                                      tCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCata                                  >  2:6030924/1‑140 (MQ=255)

                                                                                                          ggTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTc                              <  2:7230997/140‑1 (MQ=255)

                                                                                                          ggTTACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTc                              <  1:4747351/140‑1 (MQ=255)

                                                                                                             tACCACCAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTg                           <  2:6869478/140‑1 (MQ=255)

                                                                                                              accaccAGACCACTTTGACCCGGGGGGTGCCGGGTTACTGGTGTCTGCGCTGACCTGTGCCTGGCGCATCCCGTCACGCGTAGGCGAGATGGTGCATTCCGCGTGCACCGCATTACGCTCCCGTGCGGCAGATTTCTTGt                          >  2:4583367/1‑140 (MQ=255)

                                                                                                              accaccAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGt                          >  2:5677987/1‑140 (MQ=255)

                                                                                                              accaccAGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGt                          >  1:2543714/1‑140 (MQ=255)

                                                                                                                caccaGAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCg                        <  1:7135087/140‑1 (MQ=255)

                                                                                                                     gAAAACTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTAAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCaa                   <  2:6123620/140‑1 (MQ=255)

                                                                                                                      aaaaCTTTGAACAGGGGTGTTCCGTGTAAATGGTTTCTGCGCTGACCTGTGACATGCGCATCCAGTCACGCGTACTCGCAATTGTTAATTCAGATATCACAGAATTACTCTCACGTTCGGCATATTTCTTGTCGATCaaa                  >  2:4350655/1‑140 (MQ=255)

                                                                                                                         aCTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGt               <  2:1220256/140‑1 (MQ=255)

                                                                                                                         aCTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGt               >  2:8987892/1‑140 (MQ=255)

                                                                                                                          cTTTGATCCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGtt              <  2:7073357/140‑1 (MQ=255)

                                                                                                                          cTTTGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGCTCGGCATATTTCTTGTCGAGCAAAGGtt              <  1:7931683/140‑1 (MQ=255)

                                                                                                                           tttGAACCGGGGTGTGCCGGGTTAATTGCTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTc             <  1:5861750/140‑1 (MQ=255)

                                                                                                                           tttGAACCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACTCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTGGTCGAGCAAAGGTTc             <  2:3454021/140‑1 (MQ=255)

                                                                                                                               aaCCGGGGTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCa         <  2:4500936/140‑1 (MQ=255)

                                                                                                                                    gggTGTGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAga    >  2:10731016/1‑139 (MQ=255)

TTACTGTAAATAACCGCCTTCATCAAATTGGCGGAATAGTACTTCTCGTGGAAATCTTTCAGCGCCTGCTGCACCGGATTACCAGGTTTGTCGCTTAAAGTTTCGAGGTTACCACCAGAAAACTTTGAACCGGGGTGCGCCGGGTTAATGGTTTCTGCGCTGACCTGTGCCATGCGCATCCCGTCACGCGTACGCGCCATGGTTAATTCAGCGTTCACCGCATTACGCTCACGTTCGGCATATTTCTTGTCGAGCAAAGGTTCAGCAATAGCAT  >  NZ_CP025268/2960477‑2960750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`