BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,084,342	C→T	D42D (GAC→GAT)	loiP →	metalloprotease LoiP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,084,342	0	C	T	99.8%	2099.1 / NA	550	D42D (GAC→GAT)	loiP	metalloprotease LoiP
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (271/278); total (271/279)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 561 total aligned reads displayed.

GAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGACGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTCAGC  >  NZ_CP025268/3084206‑3084478

gAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGAtgcg                                                                                                                                          <  1:566697/140‑1 (MQ=255)

  agagAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCa                                                                                                                                        >  2:4752361/1‑140 (MQ=255)

  agagAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCa                                                                                                                                        <  1:2334971/140‑1 (MQ=255)

  agagAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCa                                                                                                                                        <  1:641593/140‑1 (MQ=255)

  agagAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCa                                                                                                                                        >  1:121002/1‑140 (MQ=255)

      aaaaCATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTg                                                                                                                                    <  1:8952693/140‑1 (MQ=255)

      aaaaCATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTg                                                                                                                                    >  1:4426511/1‑140 (MQ=255)

       aaaCATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGa                                                                                                                                   <  2:6032035/140‑1 (MQ=255)

            tGAAAATTCGCGCCTTATTGCTAGCAATGAGCGTGGCAACGGTACTGACAGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGTAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAAcc                                                                                                                              <  2:7873327/140‑1 (MQ=255)

             gAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAAccc                                                                                                                             >  1:9703276/1‑140 (MQ=255)

              aaaaTTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCt                                                                                                                            <  2:1195334/140‑1 (MQ=255)

               aaaTTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTg                                                                                                                           <  2:3648366/140‑1 (MQ=255)

                   tCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCg                                                                                                                       <  1:4135531/140‑1 (MQ=255)

                     gcgcCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGAt                                                                                                                     <  2:9426231/140‑1 (MQ=255)

                        ccTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAg                                                                                                                  <  2:6706469/140‑1 (MQ=255)

                          ttATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGGGTGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGc                                                                                                                <  2:388018/140‑1 (MQ=255)

                          ttATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGc                                                                                                                >  1:6652003/1‑140 (MQ=255)

                             ttGGTAGCAATGAGCGTGGCAACGGTACTGACTGGGTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGTGCGATCAGGCATg                                                                                                             <  2:4963467/140‑1 (MQ=255)

                               ggTAGCACTGAGCGTGGCAACGGTACTGACTGGGTGCCAGAATAGGGACACCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTc                                                                                                           <  1:8446716/140‑1 (MQ=255)

                               ggTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTc                                                                                                           <  1:3345339/140‑1 (MQ=255)

                               ggTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTc                                                                                                           <  1:2936356/140‑1 (MQ=255)

                               ggTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTc                                                                                                           <  1:8784633/140‑1 (MQ=255)

                                 tAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑Ag                                                                                                      >  2:8307296/1‑140 (MQ=255)

                                  aGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑Agg                                                                                                     <  1:3166407/140‑1 (MQ=255)

                                    cAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGgag                                                                                                   <  2:87337/140‑1 (MQ=255)

                                     aaTGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGgaga                                                                                                  >  2:5616547/1‑140 (MQ=255)

                                     aaTGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGgaga                                                                                                  >  2:912219/1‑140 (MQ=255)

                                       tGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATg                                                                                                >  1:9476148/1‑140 (MQ=255)

                                       tGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATg                                                                                                >  1:1957252/1‑140 (MQ=255)

                                         aGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGa                                                                                              >  1:3849293/1‑140 (MQ=255)

                                         aGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGa                                                                                              <  2:6514263/140‑1 (MQ=255)

                                           cGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACCGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACa                                                                                            >  2:4557261/1‑140 (MQ=255)

                                             tGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGc                                                                                          <  1:10861284/140‑1 (MQ=255)

                                             tGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGc                                                                                          <  2:9615755/140‑1 (MQ=255)

                                             tGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGc                                                                                          <  2:2063696/140‑1 (MQ=255)

                                                   cGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGcg                                                                                    <  2:2356162/140‑1 (MQ=255)

                                                   cGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGcg                                                                                    >  1:6302043/1‑140 (MQ=255)

                                                   cGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGcg                                                                                    >  1:2816167/1‑140 (MQ=255)

                                                       actgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGcgacga                                                                                <  2:10753726/140‑1 (MQ=255)

                                                        ctgactgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGAt                                                                               >  1:3984866/1‑140 (MQ=255)

                                                           actgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTgc                                                                            >  1:7495464/1‑140 (MQ=255)

                                                             tgGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTgcgc                                                                          >  1:5381488/1‑140 (MQ=255)

                                                             tgGTTGACAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTgcgc                                                                          <  1:1673603/140‑1 (MQ=255)

                                                              ggTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCgcc                                                                         >  1:682604/1‑140 (MQ=255)

                                                              ggTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCgcc                                                                         >  2:8913900/1‑140 (MQ=255)

                                                                ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCgccag                                                                       >  2:8871102/1‑140 (MQ=255)

                                                                ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCgccag                                                                       >  2:3057867/1‑140 (MQ=255)

                                                                ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCgccag                                                                       >  1:10747115/1‑140 (MQ=255)

                                                                ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCgccag                                                                       >  2:1828631/1‑140 (MQ=255)

                                                                ttGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCgccag                                                                       >  2:697256/1‑140 (MQ=255)

                                                                  gCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCgccagcc                                                                     >  1:9194105/1‑140 (MQ=255)

                                                                         tatGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCg                                                              >  1:7051836/1‑140 (MQ=255)

                                                                          atGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGa                                                             <  1:10445560/140‑1 (MQ=255)

                                                                           tGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGaa                                                            <  1:1266686/140‑1 (MQ=255)

                                                                              aCTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATAc                                                         >  2:9796216/1‑140 (MQ=255)

                                                                                tCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCTCAGGTGAAAACCATGAGCGATCAGGCATGTC‑‑‑AGTAGATGGACAGCAAGGCGACGATTGAGCCAGCCAATAGCGAATACGc                                                       >  1:8186415/1‑140 (MQ=255)

                                                                                 ccAACGGACTGCTCTCATCAGGAGCGGACGCTTGTCAGGCGTACAGTTGGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGGCAGCCAATCGCGACTCCGCt                                                      >  2:7352543/1‑140 (MQ=255)

                                                                                  cAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTa                                                     <  2:3988458/140‑1 (MQ=255)

                                                                                   aaCGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTaa                                                    >  1:3680398/1‑140 (MQ=255)

                                                                                   aaCGGACGGCTCTCATCAGGAGCGGAAGCTGTTCAGGCTTCCAGGTTGAGTGATGCGCAGGTGCAAACCATGAGCGTTCAGGCATGAC‑‑‑AGTAGATGGCCAGCCAGGCGAGGATTGCGGCTGCCAATAGCGTATACGCTaa                                                    >  2:2894708/1‑140 (MQ=255)

                                                                                     cGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAAc                                                  >  2:8093656/1‑140 (MQ=255)

                                                                                      ggACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACg                                                 <  1:5826043/140‑1 (MQ=255)

                                                                                       gACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGt                                                >  2:10184321/1‑140 (MQ=255)

                                                                                        aCTGCGCTCTTCAGGTGCGGAAGCGTTGCCGGGTTACAGGTTGAGTGATGCGCTGGTGAAATCCCTGCGCGATCCGGCAGGTC‑‑‑AGGAGATGGAGAGCTAGGCGACGATTGCGCCAGCCACTTGCGAATCCGCTATTCGTc                                               >  2:6247859/1‑140 (MQ=255)

                                                                                         cTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCt                                              <  2:4409721/140‑1 (MQ=255)

                                                                                          tGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAg                                                                                           >  2:3491975/1‑94 (MQ=255)

                                                                                           gCTCGCCTCAGGATCTGATGTTTTTGAGGCTGACTGTTTTCGTGTTGCGCCGTTGATCACCATGATCTTTATGGCTTGTT‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGa                                            <  2:4334080/140‑1 (MQ=255)

                                                                                            ctctCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGAc                                           >  2:3279304/1‑140 (MQ=255)

                                                                                            ctctCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGAc                                           >  2:1566375/1‑140 (MQ=255)

                                                                                            ctctCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGAc                                           >  1:3518883/1‑140 (MQ=255)

                                                                                             tctcATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACa                                          <  1:6101972/140‑1 (MQ=255)

                                                                                             tctcATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGAC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAAAACGCTAAACGTCTGACa                                          >  1:2553854/1‑140 (MQ=255)

                                                                                               tcatcaGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAAc                                        >  2:8457936/1‑140 (MQ=255)

                                                                                                     ggAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGc                                  <  1:9343494/140‑1 (MQ=255)

                                                                                                     ggAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGc                                  <  2:1694582/140‑1 (MQ=255)

                                                                                                      gAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGcc                                 >  1:10613779/1‑140 (MQ=255)

                                                                                                         cGGAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAAt                              >  1:8028854/1‑140 (MQ=255)

                                                                                                         cGGAAGCTTTTAAGGCTTACAGTTTGATTGATGCGCAGGTGAAAACCCTGAGAGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAAGACGCTAATCGTCTGACAACTATTGCCAAt                              >  2:177794/1‑140 (MQ=255)

                                                                                                           gAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgc                            >  1:10061708/1‑140 (MQ=255)

                                                                                                           gAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgc                            >  2:10395032/1‑140 (MQ=255)

                                                                                                           gAAGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgc                            >  2:216414/1‑140 (MQ=255)

                                                                                                             aGCTTTTCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATgcgc                          <  2:7473682/140‑1 (MQ=255)

                                                                                                                ttttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAg                       <  2:10893335/140‑1 (MQ=255)

                                                                                                                 tttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAgg                      <  1:8875009/140‑1 (MQ=255)

                                                                                                                  ttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGc                     <  1:501914/140‑1 (MQ=255)

                                                                                                                  ttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGc                     <  2:2678138/140‑1 (MQ=255)

                                                                                                                  ttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGc                     <  2:582735/140‑1 (MQ=255)

                                                                                                                  ttCAGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGc                     <  1:9856807/140‑1 (MQ=255)

                                                                                                                     aGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCTACGATTGCGCCAGCCAATAGCGAATACTCTAAACGTCTGACAACTATTGCCATTGCGCTAGGcaac                  <  2:2567186/140‑1 (MQ=255)

                                                                                                                     aGGCTTACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGcaac                  <  1:2650891/140‑1 (MQ=255)

                                                                                                                         ttACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAata              >  1:472388/1‑140 (MQ=255)

                                                                                                                          tACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAatat             >  1:861937/1‑140 (MQ=255)

                                                                                                                          tACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAatat             >  1:7811493/1‑140 (MQ=255)

                                                                                                                          tACAGTTTGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAatat             >  2:6367984/1‑140 (MQ=255)

                                                                                                                               tttGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAATCGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACg        >  2:10485773/1‑140 (MQ=255)

                                                                                                                                ttGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACgg       >  1:6898590/1‑140 (MQ=255)

                                                                                                                                 tGAGTGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGt      >  2:526175/1‑140 (MQ=255)

                                                                                                                                     tGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTCAGGAGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTc     <  2:5406061/140‑1 (MQ=255)

                                                                                                                                     tGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTCAGc  >  1:4626152/1‑140 (MQ=255)

                                                                                                                                     tGATGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTCAGc  <  1:10234733/140‑1 (MQ=255)

GAAGAGAAAACATGAAAATTCGCGCCTTATTGGTAGCAATGAGCGTGGCAACGGTACTGACTGGTTGCCAGAATATGGACTCCAACGGACTGCTCTCATCAGGAGCGGAAGCTTTTCAGGCTTACAGTTTGAGTGACGCGCAGGTGAAAACCCTGAGCGATCAGGCATGTC‑‑‑AGGAGATGGACAGCAAGGCGACGATTGCGCCAGCCAATAGCGAATACGCTAAACGTCTGACAACTATTGCCAATGCGCTAGGCAACAATATCAACGGTCAGC  >  NZ_CP025268/3084206‑3084478

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`