BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,137,127	A→G	N231S (AAT→AGT)	yghT →	dTMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,137,127	0	A	G	99.5%	2072.4 / ‑5.0	601	N231S (AAT→AGT)	yghT	dTMP kinase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (294/303); minor base C (0/3); total (295/306)
Fisher's exact test for biased strand distribution p-value = 2.49e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 601 total aligned reads displayed.

AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGCT  >  NZ_CP025268/3136991‑3137262

aaGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTc                                                                                                                                       <  2:129369/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACTACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:8952453/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  1:4771240/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  1:10197131/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:8492723/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:689928/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:5719950/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:2152569/140‑1 (MQ=255)

                 acaGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCg                                                                                                                      <  2:1480349/140‑1 (MQ=255)

                  caGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGa                                                                                                                     >  1:965740/1‑140 (MQ=255)

                  caGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGa                                                                                                                     >  1:2444991/1‑140 (MQ=255)

                     ttAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCg                                                                                                                  >  1:4457855/1‑140 (MQ=255)

                     ttAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCg                                                                                                                  >  1:10512873/1‑140 (MQ=255)

                      tcaCCTTTGGTGGTGCACAACTGGTTGATATCGCGGCCACTCAGACACTGGACCATGAGTTGGTTGATGCAGAAAAAGCGAGTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGc                                                                                                                 <  2:10535166/138‑1 (MQ=255)

                      tAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGc                                                                                                                 >  2:2499001/1‑140 (MQ=255)

                      tAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGc                                                                                                                 <  1:9923229/140‑1 (MQ=255)

                            ttGGTGGTGCACAACTGGTTGATATCGATGCTAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                                           >  1:6810219/1‑140 (MQ=255)

                            ttGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAc                                                                                                           >  2:9689056/1‑140 (MQ=255)

                             tggtggTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGAcc                                                                                                          >  2:9860079/1‑140 (MQ=255)

                                   tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGTCCGGGcct                                                                                                    >  1:9134118/1‑138 (MQ=255)

                                   tgCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAt                                                                                                    <  1:9293868/140‑1 (MQ=255)

                                    gCACAACTGGTTGATCTCGCTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                   <  2:5926870/140‑1 (MQ=255)

                                    gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                   >  2:3333799/1‑140 (MQ=255)

                                    gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                   >  1:5960887/1‑140 (MQ=255)

                                    gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                   >  2:4436110/1‑140 (MQ=255)

                                    gCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAtt                                                                                                   >  2:4703558/1‑140 (MQ=255)

                                     cacaACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCAttt                                                                                                  >  1:3712346/1‑140 (MQ=255)

                                          ccggTGGATATCGATGCCACTCAGCCACTGGAACAGGGGTTGGTTGATGCAGAACAAGCGATTACCGAATTTAGGACCGCGC‑GTGGTTATCACTAGTCAAATATGGAAATGCCCGATCGCCAGGACCGGGCCTTTTCAgg                                                                                             <  2:7942880/138‑1 (MQ=255)

                                          cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                             <  2:139596/140‑1 (MQ=255)

                                          cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                             <  1:2143307/140‑1 (MQ=255)

                                          cTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAgg                                                                                             >  1:7020080/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  1:3574699/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  1:9040182/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  2:4812989/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  2:8364417/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGA‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAg                                                                                          >  2:7669475/1‑140 (MQ=255)

                                             gTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGAGTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTGTCAGGAAg                                                                                          >  1:1990227/1‑140 (MQ=255)

                                                  tatCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTaa                                                                                     >  1:5562039/1‑140 (MQ=255)

                                                    tCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTATCAGGACGGTTAAAt                                                                                   >  2:7483993/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                >  1:7786033/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCaa                                                                                >  1:8516400/1‑140 (MQ=255)

                                                       aTGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCACGATCGCCAGGACCGGGCATTTTCAGTAAGGAtaaataaa                                                                                >  1:5675230/1‑140 (MQ=255)

                                                        tGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAAc                                                                               >  2:2000205/1‑140 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  1:10719213/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  1:937005/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  1:878806/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  2:6045471/140‑1 (MQ=255)

                                                          ccAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTg                                                                             <  2:8806102/140‑1 (MQ=255)

                                                           aaaTCAGCCACGGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAATTGCCCGATCGCCAGGACCGGGCATTTTCAGGACGGTTAAATCAACTGc                                                                            >  2:7058213/2‑140 (MQ=255)

                                                            aaTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGT‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                                           <  2:3916269/140‑1 (MQ=255)

                                                            aaTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCa                                                                           <  1:5385777/140‑1 (MQ=255)

                                                             aTCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCaa                                                                          <  2:3686200/140‑1 (MQ=255)

                                                               cAGCCACTGGAACCGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  2:2293695/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:8461733/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:814461/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:7857895/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  2:8213397/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:1467867/140‑1 (MQ=255)

                                                               cAGCCACTGGAACAGGTGATGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAACTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATg                                                                        <  1:8018769/140‑1 (MQ=255)

                                                                 gCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTGTCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCt                                                                      <  1:4249031/140‑1 (MQ=255)

                                                                  ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                     <  2:10401529/140‑1 (MQ=255)

                                                                  ccACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTa                                                                     >  1:10874256/1‑140 (MQ=255)

                                                                      tGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCa                                                                 >  2:2891739/1‑140 (MQ=255)

                                                                        gAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGt                                                               >  1:957955/1‑140 (MQ=255)

                                                                         aaCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTa                                                              <  2:316085/140‑1 (MQ=255)

                                                                          aCAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCAGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTAc                                                             >  1:8686607/1‑140 (MQ=255)

                                                                           cAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACa                                                            <  1:6483986/140‑1 (MQ=255)

                                                                            aGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAg                                                           >  2:554312/1‑140 (MQ=255)

                                                                              gtgtTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGcc                                                         >  2:1723944/1‑140 (MQ=255)

                                                                              gtgtTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGcc                                                         >  2:6503515/1‑140 (MQ=255)

                                                                                gttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTATATCAACTGCAATGCTATCCAGTACAGCcca                                                       >  2:5449337/1‑140 (MQ=255)

                                                                                 ttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccac                                                      <  2:7809504/140‑1 (MQ=255)

                                                                                 ttggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccac                                                      <  1:2291194/140‑1 (MQ=255)

                                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                     >  1:5143489/1‑140 (MQ=255)

                                                                                  tggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacc                                                     <  2:10733601/140‑1 (MQ=255)

                                                                                   ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATTCACGATCGCCAGGACCGGGCATGTTCAGGATGGTGAAATCAACTGCAATGCTATCCATTACAGCcaacca                                                    >  2:7312947/1‑140 (MQ=255)

                                                                                   ggttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCccacca                                                    <  1:6133358/140‑1 (MQ=255)

                                                                                     ttgATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCaga                                                  <  1:9820093/140‑1 (MQ=255)

                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                               >  1:3330426/1‑140 (MQ=255)

                                                                                        aTGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTGCAGGAAGGTGAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaag                                               >  2:5102471/1‑140 (MQ=255)

                                                                                         tGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCagaaaga                                              >  2:427320/1‑140 (MQ=255)

                                                                                             gAAAAAGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGaaaaa                                          <  1:2693748/140‑1 (MQ=255)

                                                                                               aaaaGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAAtt                                        >  1:343830/1‑140 (MQ=255)

                                                                                                  aGCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTgcc                                     <  2:1025230/140‑1 (MQ=255)

                                                                                                   gCGATTACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATTCTATCCAGTACAGCCCACCAGAAAGAAAAATTgccg                                    >  1:6190164/1‑140 (MQ=255)

                                                                                                        tACGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgt                               <  1:3849837/140‑1 (MQ=255)

                                                                                                         actgATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgta                              <  2:9325004/137‑1 (MQ=255)

                                                                                                         acggcatttATGACCCCGCGG‑GGTTATCACTACTCAAAACGGGACAGGCCCCCTCGCCAGGACCCGGCATCTTCAGGATGGATAAATCAACTGCAATGCTAACCAGTACATCCCACCTGAAAGAAAAACTGCCGCCGgta                              <  2:1122208/134‑1 (MQ=255)

                                                                                                          cGGATTTTATGACCGCGC‑GTGGTTATCACTAGTCAAAACTGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGgtaa                             <  2:3480805/140‑1 (MQ=255)

                                                                                                                  ctGACCGCGC‑GTGGTGATCACTAGTCAAAACTGGAAAGGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATa                     <  2:9491166/139‑1 (MQ=255)

                                                                                                                  aTGACCGCGC‑GTGGTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATa                     <  1:129476/140‑1 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTACAGTAAATACCCACGc              >  1:2950278/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc              <  1:4058182/135‑1 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc              >  1:7148111/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGc              >  1:1611635/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGAAAAGTAAATACCCACGc              >  1:2041962/6‑140 (MQ=255)

                                                                                                                          cgcgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCAAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAATATTGCCGCCGGTAAAGTAAATACCCACGc              >  2:3101365/6‑140 (MQ=255)

                                                                                                                            cgtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCCGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTCAAGTACATACCCACGCCa            >  2:5359913/4‑140 (MQ=255)

                                                                                                                             gtggTTATCACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCAt           <  1:7556963/138‑1 (MQ=255)

                                                                                                                                     cACTAGTCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGc   >  1:6600681/1‑140 (MQ=255)

AAGATTGCCATAACGCCACAGTTAACCTTTGGTGGTGCACAACTGGTTGATATCGATGCCAATCAGCCACTGGAACAGGTGTTGGTTGATGCAGAAAAAGCGATTACGGATTTTATGACCGCGC‑G‑GGTTATCACTAATCAAAAATGGAAATGCCCGATCGCCAGGACCGGGCATTTTCAGGAAGGTTAAATCAACTGCAATGCTATCCAGTACAGCCCACCAGAAAGAAAAATTGCCGCCGGTAAAGTAAATACCCACGCCATCAGGATGCT  >  NZ_CP025268/3136991‑3137262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`