BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,179,162	T→C	141 (TAA→TAG)	yqiB ←	DUF1249 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,179,162	0	T	C	99.8%	1980.2 / NA	568	141 (TAA→TAG)	yqiB	DUF1249 family protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (306/261); minor base A (0/1); total (306/262)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 568 total aligned reads displayed.

TGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGCG  >  NZ_CP025268/3179026‑3179296

 gTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCCAATGGTGTCCTTAGGTTTCACGACGCtaat                                                                                                                                    >  2:4173308/1‑140 (MQ=255)

 gTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTACAAATGTTGTCCTTAGGTTTCACGACGCtaat                                                                                                                                    >  2:4652613/1‑140 (MQ=255)

  tttACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCtaata                                                                                                                                   <  2:2837842/140‑1 (MQ=255)

    tACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAGTCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATaaa                                                                                                                                 >  1:6729572/1‑140 (MQ=255)

    tACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATaaa                                                                                                                                 <  1:10313796/140‑1 (MQ=255)

    tACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATaaa                                                                                                                                 <  1:5368342/140‑1 (MQ=255)

    tACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATaaa                                                                                                                                 >  1:2805287/1‑140 (MQ=255)

     aCCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAAc                                                                                                                                >  1:6956295/1‑140 (MQ=255)

     aCCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAAc                                                                                                                                >  2:753075/1‑140 (MQ=255)

          tAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTTTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGaa                                                                                                                           >  1:195801/1‑140 (MQ=255)

                ggCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTACAATCCTGACTCTGGCCTCACCAGCGAGAGGTAGGGTTAACAGGCTTTCCAAATGGTGTACTTAGGGTTATCGACGCTAATAAACCGGAATCGccc                                                                                                                     >  2:6459017/1‑139 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCTGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTCATAAACCGGAATCGCCAt                                                                                                                    >  1:7341842/1‑140 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCAt                                                                                                                    <  1:3458114/140‑1 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCAt                                                                                                                    >  1:4363824/1‑140 (MQ=255)

                 gCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCAt                                                                                                                    >  1:906943/1‑140 (MQ=255)

                  cTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATc                                                                                                                   <  1:2982571/140‑1 (MQ=255)

                  cTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATc                                                                                                                   <  2:2176610/140‑1 (MQ=255)

                   ttCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCg                                                                                                                  >  1:3161509/1‑140 (MQ=255)

                   ttCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCg                                                                                                                  <  2:5257798/140‑1 (MQ=255)

                    tCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGc                                                                                                                 >  2:560030/1‑140 (MQ=255)

                     cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCTCGACGCTAATAAACCGGAATCGCCATCGCt                                                                                                                >  1:8170121/1‑140 (MQ=255)

                     cGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCt                                                                                                                >  2:4874871/1‑140 (MQ=255)

                      gTGCTTTTGTGAAAAAAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCATCCATAGGAAGGGTTAAAAGGCTTTCCAAATGTTGTCCTTTGGTTTCACGACGCTAATAAAACGGAATCGCCATCGCTc                                                                                                               >  2:4282169/1‑140 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAACTGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                              <  2:9398308/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                              <  1:228463/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                              <  1:7688610/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                              <  1:2148252/140‑1 (MQ=255)

                       tGCTTTTGTGCAAACAGGAGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAACTGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTcc                                                                                                              <  2:160751/140‑1 (MQ=255)

                        gCTTTTGTGCAAACAGGTGAGTGTCGGTAATGTGTAACATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCa                                                                                                             <  2:8122381/140‑1 (MQ=255)

                            ttGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCAtgtg                                                                                                         <  1:8770102/140‑1 (MQ=255)

                                 tataCAGGTGAGTGTCTGTAATTTGTAAAATCCTTACTATTGCCTAACCAGCCAGAGGAAGTGTTAACTGTCTTTACAAATGGTTTCCTTAGGTTTCACGACGCTTATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                    <  1:1879080/137‑1 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                    <  1:10128821/140‑1 (MQ=255)

                                 cAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTaaa                                                                                                    <  1:3576587/140‑1 (MQ=255)

                                  aaaCAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAAc                                                                                                   <  2:10214018/140‑1 (MQ=255)

                                   ctcAGGTGAGTGTCCGTACATAGTACACTCCTGACTCCGGCCCCCCCACCCAGACGACGGGCTAACAGGCTTTCCAACTGCTGTCCATAGCTTACCCGACGCTCATAAACCGGAATCGCCATCGCTCCAAGTGCTAAACa                                                                                                  <  2:10103021/138‑1 (MQ=255)

                                   accAGGTGAGTGTCGGTAATTTGTAAAANCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACa                                                                                                  <  1:5876475/138‑1 (MQ=255)

                                        gTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATc                                                                                             >  1:10601463/1‑140 (MQ=255)

                                                cGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAg                                                                                     >  1:4438132/1‑140 (MQ=255)

                                                cGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAg                                                                                     >  1:4732228/1‑140 (MQ=255)

                                                 ggTACTTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAAACGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGt                                                                                    <  2:382404/140‑1 (MQ=255)

                                                  gTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTc                                                                                   >  2:5391106/1‑140 (MQ=255)

                                                  gTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTc                                                                                   >  2:8998427/1‑140 (MQ=255)

                                                           aaaaTCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTaaaaa                                                                          <  2:4474880/140‑1 (MQ=255)

                                                           aaaaTCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTaaaaa                                                                          <  1:7795219/140‑1 (MQ=255)

                                                             aaTCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACt                                                                        >  2:1491718/1‑140 (MQ=255)

                                                                ccTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGAt                                                                     <  1:597573/140‑1 (MQ=255)

                                                                       ctGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTg                                                              <  2:2008512/140‑1 (MQ=255)

                                                                       ctGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTg                                                              <  1:10444199/140‑1 (MQ=255)

                                                                         ggCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGAt                                                            >  2:6106761/1‑140 (MQ=255)

                                                                         ggCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGAt                                                            >  1:117340/1‑140 (MQ=255)

                                                                         ggCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGAt                                                            >  2:10662973/1‑140 (MQ=255)

                                                                          gCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAATATCTGAGTAATTTGATg                                                           >  1:10531523/1‑140 (MQ=255)

                                                                              cACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCttt                                                       <  2:3378584/140‑1 (MQ=255)

                                                                                    ccagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTtcgtcg                                                 >  1:5265594/1‑140 (MQ=255)

                                                                                    ccagAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCAGCTAAAAACTGATTAATTTGATGCTTTtcgtcg                                                 >  2:7263693/1‑140 (MQ=255)

                                                                                       gagGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGt                                              <  1:1713240/140‑1 (MQ=255)

                                                                                         ggAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTg                                            >  2:7825303/1‑140 (MQ=255)

                                                                                             gggTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGc                                        >  2:4417858/1‑140 (MQ=255)

                                                                                                  aaCAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACtt                                   >  1:7553270/1‑140 (MQ=255)

                                                                                                      tgCTTTCCAAATGGTGTCCTTATGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTtta                               <  1:2597665/139‑1 (MQ=255)

                                                                                                      ggCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAACCCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTtta                               <  2:1237683/140‑1 (MQ=255)

                                                                                                       gCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttat                              <  2:9544544/140‑1 (MQ=255)

                                                                                                       gCTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttat                              <  1:9943472/140‑1 (MQ=255)

                                                                                                       gCTTTCCAAAGGGTGTCCTTAGGTTTCACGACGCTACTAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttat                              <  2:5858602/140‑1 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                             <  1:7115437/140‑1 (MQ=255)

                                                                                                        cTTTCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTttatt                             >  2:3061635/1‑140 (MQ=255)

                                                                                                          ttCCACATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           <  1:6425337/140‑1 (MQ=255)

                                                                                                          ttCCAACTGGTGTCCTTAGGTTTAACGACGCTAATCAACCGGAATCGCCATCGCTCCATGTGCTACACAGTATCGCAACCAGTCCGCTAAAAACTTATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           <  2:8428989/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGCCTCTCATACACCGGAATCGACATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTTATTTGTTGCTGTTCGTCGCGTTGATGCTCCTTTTTATTAg                           <  2:2573338/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATCAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           <  1:8028288/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           <  2:8921710/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           >  1:375383/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           >  2:10832070/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           <  2:8223325/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           <  1:6111767/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           >  1:8667733/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           <  1:8840552/140‑1 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAg                           >  2:2085274/1‑140 (MQ=255)

                                                                                                          ttCCAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTATTTATTAg                           <  2:2362526/140‑1 (MQ=255)

                                                                                                          gtCCACATGGTGACCTTACCATTCACGACGCTAATACACCGGAATCGCCATCGCTCCATGTGCTACCCAGTCACGCAACCCGTCCGCTAAAAACAGCTTACTTTGATGCTTTTCGTCGCGTAGCTGCAACTTTTTATTAg                           <  2:3482930/139‑1 (MQ=255)

                                                                                                            ccAACTGGTGTCCTTACGTTTCACGACGCGAAAAAACCGGACTCGCCATCGCTCCCTGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                         <  2:10520587/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                         <  2:5677931/140‑1 (MQ=255)

                                                                                                            ccAAATGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGa                         <  1:9725420/140‑1 (MQ=255)

                                                                                                               aaTGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATaa                      <  2:7386618/140‑1 (MQ=255)

                                                                                                                 tGGTGTCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATc                    >  1:934231/1‑140 (MQ=255)

                                                                                                                   gtgtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCATCTTTTTATTAGGATAATCAt                  >  1:126338/1‑140 (MQ=255)

                                                                                                                   gtgtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCAt                  >  2:1778288/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGACTCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCACCTTTTTATTAGGATAATCATAc                >  2:5955645/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAc                >  2:5039151/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAc                >  2:2640001/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAc                >  1:4141102/1‑140 (MQ=255)

                                                                                                                     gtCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAc                >  2:5521938/1‑140 (MQ=255)

                                                                                                                      tCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATaac               >  1:10737296/1‑138 (MQ=255)

                                                                                                                      tCCTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATAcc               >  2:6579840/1‑140 (MQ=255)

                                                                                                                       ccTTAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACcg              >  1:153505/1‑140 (MQ=255)

                                                                                                                         ttAGGTTTCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACcgcg            <  1:9526965/140‑1 (MQ=255)

                                                                                                                               ttCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGa      <  1:5728430/140‑1 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa     >  2:7071891/1‑140 (MQ=255)

                                                                                                                                tCACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGaa     >  2:1880624/1‑140 (MQ=255)

                                                                                                                                 cACGACGCTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAg    >  1:8290816/1‑140 (MQ=255)

TGTTTACCCCTAACAGGGCTTCGTGCTTTTGTGCAAACAGGTGAGTGTCGGTAATTTGTAAAATCCTGACTCTGGCCTCACCAGCCAGAGGAAGGGTTAACAGGCTTTCCAAATGGTGTCCTTAGGTTTCACGACGTTAATAAACCGGAATCGCCATCGCTCCATGTGCTAAACAGTATCGCAACCAGTCCGCTAAAAACTGATTAATTTGATGCTTTTCGTCGCGTTGATGCAACTTTTTATTAGGATAATCATACCGCGCTTTGAAGCG  >  NZ_CP025268/3179026‑3179296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`