BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,567,287	T→C	478 (TAA→TAG)	glgA ←	glycogen synthase GlgA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,567,287	0	T	C	99.7%	2023.7 / NA	583	478 (TAA→TAG)	glgA	glycogen synthase GlgA
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (322/258); minor base A (1/1); total (324/259)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 585 total aligned reads displayed.

TCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAATTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGCC  >  NZ_CP025268/3567150‑3567422

    gTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTc                                                                                                                                   >  1:397424/1‑140 (MQ=255)

    gTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTc                                                                                                                                   >  2:4261820/1‑140 (MQ=255)

    gTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTc                                                                                                                                   >  2:2934510/1‑140 (MQ=255)

       tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                <  2:6017101/140‑1 (MQ=255)

       tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                <  1:6496937/140‑1 (MQ=255)

       tATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAg                                                                                                                                <  1:838238/140‑1 (MQ=255)

        aTTGGCGACGCCCGGGGCCTATCCCAACGTAACCCTCAGCTAGAAAGCGATAGAGTGCTTCAGCTCTTCTACGCTAAGCGTGGGCGATGAATAGGTAAACGGAGCATTCATATAGGCGGTTCCTGAAAACTATTTCAAGc                                                                                                                               <  2:9387580/140‑1 (MQ=255)

         ttGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCg                                                                                                                              >  1:8823349/1‑140 (MQ=255)

            gCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATa                                                                                                                           >  2:3812934/1‑140 (MQ=255)

                    cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt                                                                                                                   <  1:211530/140‑1 (MQ=255)

                    cGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCt                                                                                                                   <  1:8916401/140‑1 (MQ=255)

                           tttCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTa                                                                                                            >  1:5704220/1‑140 (MQ=255)

                                 aTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACtt                                                                                                      >  1:1209978/1‑140 (MQ=255)

                                 aTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACtt                                                                                                      >  1:10527538/1‑140 (MQ=255)

                                 aTCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACtt                                                                                                      >  2:3232777/1‑140 (MQ=255)

                                       aaCATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATa                                                                                                                           >  1:10742652/1‑113 (MQ=255)

                                       aaCATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTcgccgc                                                                                                >  2:2558122/1‑140 (MQ=255)

                                        aCATCAGCTTGTAAGCGATAGTGTGCTTACGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCTTTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCcgcg                                                                                               <  2:611378/140‑1 (MQ=255)

                                          aTCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGAc                                                                                             <  1:4888393/140‑1 (MQ=255)

                                            cAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCt                                                                                           >  1:1597108/1‑140 (MQ=255)

                                            cAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCt                                                                                           >  1:2333002/1‑140 (MQ=255)

                                            cAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGAGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCAGCGACCt                                                                                           >  2:9942624/1‑140 (MQ=255)

                                               cTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCAGATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGcc                                                                                        >  1:6333772/1‑140 (MQ=255)

                                                 tGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAg                                                                                      >  1:6422629/1‑140 (MQ=255)

                                                   tAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCt                                                                                    >  1:3233693/1‑140 (MQ=255)

                                                    aaGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTa                                                                                   <  1:2566061/140‑1 (MQ=255)

                                                         aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  2:7883997/1‑140 (MQ=255)

                                                         aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  1:3798083/1‑140 (MQ=255)

                                                         aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  1:3444877/1‑140 (MQ=255)

                                                         aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  2:1160484/1‑140 (MQ=255)

                                                         aTAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGAGAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATc                                                                              >  2:10710768/1‑140 (MQ=255)

                                                          tAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATcc                                                                             >  2:5385751/1‑140 (MQ=255)

                                                           agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                            <  2:10163922/140‑1 (MQ=255)

                                                           agagTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCa                                                                            <  1:3639196/140‑1 (MQ=255)

                                                             agTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCAtt                                                                          >  2:4403862/1‑140 (MQ=255)

                                                             agTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCAtt                                                                          >  2:1916037/1‑140 (MQ=255)

                                                                 cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      >  2:9288184/1‑140 (MQ=255)

                                                                 cTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCa                                                                      >  2:10364690/1‑140 (MQ=255)

                                                                  ttAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCAt                                                                     >  2:5831232/1‑140 (MQ=255)

                                                                    aaGAGCTTCTACGCTAAGCGTGGGTGATGAATATGTAAACGGAGCATTCATATAGGCGTGTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAg                                                                   >  2:2355552/1‑140 (MQ=255)

                                                                    aaGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGANCTGCCAGCTAAAATCCATTGCCATAg                                                                   >  1:6667739/1‑140 (MQ=255)

                                                                    aaGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAg                                                                   >  1:9957033/1‑140 (MQ=255)

                                                                      gagCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGcc                                                                 <  2:1331890/140‑1 (MQ=255)

                                                                       agCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCt                                                                >  1:422314/1‑140 (MQ=255)

                                                                         cTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGa                                                              >  1:7616117/1‑140 (MQ=255)

                                                                          ttCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGGTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGTTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGAc                                                             <  2:3562144/140‑1 (MQ=255)

                                                                           tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCg                                                                                                                              <  2:5003686/74‑1 (MQ=255)

                                                                           tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACt                                                                                                       >  2:777869/1‑97 (MQ=255)

                                                                           tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACg                                                            >  1:7187775/1‑140 (MQ=255)

                                                                           tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACg                                                            >  1:409487/1‑140 (MQ=255)

                                                                           tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACg                                                            >  2:6941314/1‑140 (MQ=255)

                                                                           tCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCAGTGCCATAGCCTGACg                                                            >  2:8638485/1‑140 (MQ=255)

                                                                                gCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTACAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCAGACGTTGca                                                       <  2:3495021/140‑1 (MQ=255)

                                                                                    aGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTCACGTTGCACaaa                                                   >  2:7230174/1‑140 (MQ=255)

                                                                                      cGTGGGCGATGACTATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCAAAGCCTGACGTTGCACAAAcc                                                 >  2:8376240/1‑140 (MQ=255)

                                                                                        tGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGc                                               <  2:8872405/140‑1 (MQ=255)

                                                                                         gggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTTCCAGCTAAATTCCATTGCCATCGCCTGACGTTGCTCAAACCGcc                                              >  2:9816193/1‑140 (MQ=255)

                                                                                          ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             >  2:7671040/1‑140 (MQ=255)

                                                                                          ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             >  2:8069787/1‑140 (MQ=255)

                                                                                          ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             >  2:6799157/1‑140 (MQ=255)

                                                                                          ggCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCca                                             <  2:9195751/140‑1 (MQ=255)

                                                                                             gATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAg                                          <  1:9656249/140‑1 (MQ=255)

                                                                                               tGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTg                                        <  1:9283838/140‑1 (MQ=255)

                                                                                                gAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGa                                       <  2:9550754/140‑1 (MQ=255)

                                                                                                  atatGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAg                                     <  1:2224656/140‑1 (MQ=255)

                                                                                                  atatGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACCACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAg                                     <  1:6161282/140‑1 (MQ=255)

                                                                                                    atGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGa                                   <  1:10373729/140‑1 (MQ=255)

                                                                                                     tGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGAc                                  >  1:10909089/1‑140 (MQ=255)

                                                                                                     tGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAAACGCCACAGTGAAGGAc                                  >  1:9180155/1‑140 (MQ=255)

                                                                                                       tAAACGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACgg                                <  1:10018603/140‑1 (MQ=255)

                                                                                                         aaCGGAGCATTCATATATGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCAGTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGa                              <  2:5596252/140‑1 (MQ=255)

                                                                                                          aCGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAc                             <  2:3090043/140‑1 (MQ=255)

                                                                                                           cGGAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGAcc                            >  1:4548329/1‑140 (MQ=255)

                                                                                                            ggAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACca                           <  1:504758/140‑1 (MQ=255)

                                                                                                             gAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcac                          >  1:8103427/1‑140 (MQ=255)

                                                                                                             gAGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcac                          <  1:5189730/140‑1 (MQ=255)

                                                                                                             gAGCATTCATATAGGCGTTACCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGACAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcac                          <  2:6425603/140‑1 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGc                                               <  2:2061250/118‑1 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                         >  1:8488128/1‑140 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                         >  1:1810553/1‑140 (MQ=255)

                                                                                                              aGCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACcaca                         <  2:397424/140‑1 (MQ=255)

                                                                                                               gCATTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAg                        >  1:7895270/1‑140 (MQ=255)

                                                                                                                 aTTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACNGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTa                      >  1:10137599/1‑140 (MQ=255)

                                                                                                                 aTTCATATAGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTa                      >  1:743283/1‑140 (MQ=255)

                                                                                                                        taGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc               >  2:7568806/1‑140 (MQ=255)

                                                                                                                        taGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc               <  1:9495419/140‑1 (MQ=255)

                                                                                                                        taGGCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGc               >  2:8461616/1‑140 (MQ=255)

                                                                                                                          ggCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCAc             >  1:5380405/1‑140 (MQ=255)

                                                                                                                           gCGTTTCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACg            >  1:2720911/1‑140 (MQ=255)

                                                                                                                             gTTTCCTGAAAACTATAACAAGAGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATAGCCATAGCCTGACGTTTCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACgac          >  1:4623029/1‑138 (MQ=255)

                                                                                                                               ttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGa        >  1:6041749/1‑140 (MQ=255)

                                                                                                                               ttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGa        >  1:6940399/1‑140 (MQ=255)

                                                                                                                               ttCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGAAGGGACCACAGTACAAAAGCACGTCGa        >  2:6616446/1‑140 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  1:2882865/140‑1 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       >  1:1984628/1‑140 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       <  2:10832909/140‑1 (MQ=255)

                                                                                                                                tCCTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGaa       >  2:170556/1‑140 (MQ=255)

                                                                                                                                  cTGAAAACTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATa     <  1:4721775/140‑1 (MQ=255)

                                                                                                                                     aaaaCTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTCCAAAAGCACGTCGAATAGcc  >  2:4622018/1‑140 (MQ=255)

                                                                                                                                     aaaaCTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGcc  >  2:743190/1‑140 (MQ=255)

TCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAAACATCAGCTTGTAAGCGATAGAGTGCTTAAGAGCTTCTACGCTAAGCGTGGGCGATGAATATGTAAACGGAGCATTCATATAGGCGTTTCCTGAAAATTATTTCAAGCGATAGTAAAGCTCACGGTACGACTTCGCCGCGACCTGCCAGCTAAAATCCATTGCCATAGCCTGACGTTGCACAAACCGCCACAGTGAAGGACGGGACCACAGTACAAAAGCACGTCGAATAGCC  >  NZ_CP025268/3567150‑3567422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`