BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,844,378	T→C	445 (TAA→TAG)	adeQ ←	adenine permease AdeQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,844,378	0	T	C	99.7%	2061.5 / NA	594	445 (TAA→TAG)	adeQ	adenine permease AdeQ
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (246/346); minor base A (2/0); total (248/346)
Fisher's exact test for biased strand distribution p-value = 1.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 597 total aligned reads displayed.

AATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGATCG  >  NZ_CP025268/3844242‑3844514

aaTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTaa                                                                                                                                       <  1:10143007/140‑1 (MQ=255)

aaTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTaa                                                                                                                                       <  1:9123371/140‑1 (MQ=255)

aaTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTaa                                                                                                                                       <  1:2336158/140‑1 (MQ=255)

 aTCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAAt                                                                                                                                      <  1:10044685/140‑1 (MQ=255)

  tCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATc                                                                                                                                     >  1:8225197/1‑140 (MQ=255)

     cTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCCCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccac                                                                                                                                  <  2:1430067/140‑1 (MQ=255)

     cTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccac                                                                                                                                  <  1:4915313/140‑1 (MQ=255)

     cTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccac                                                                                                                                  <  1:918798/140‑1 (MQ=255)

     cTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccac                                                                                                                                  <  1:3639744/140‑1 (MQ=255)

       tttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccacca                                                                                                                                <  2:496596/140‑1 (MQ=255)

       tttCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATccacca                                                                                                                                <  1:9279072/140‑1 (MQ=255)

            ggCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGaata                                                                                                                           <  2:2047213/140‑1 (MQ=255)

                ccGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCACATCAAGAGTTCTCCTGCGTAATCAGAACTCAAGATATCTGGAAACCCTCGCCAGTTAATAAACCACCAGAAAActct                                                                                                                       >  1:6989859/1‑140 (MQ=255)

                  ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTc                                                                                                                     <  1:5581982/140‑1 (MQ=255)

                  ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTc                                                                                                                     >  2:9378914/1‑140 (MQ=255)

                  ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTc                                                                                                                     <  1:10619810/140‑1 (MQ=255)

                  ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTc                                                                                                                     <  1:4050945/140‑1 (MQ=255)

                  ggATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTc                                                                                                                     <  1:9437977/140‑1 (MQ=255)

                  ggAGGTCAGCACCCGCAAACCAACCACAAACACGACCGACAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCTCCAGTTACTAATCCACCAGAATAATCTTc                                                                                                                     <  2:7922345/140‑1 (MQ=255)

                       tCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCTTAATCAGAACTCATGATATCTGGAAACCCTCGCAAGTTACTAATCCACCAGAATAATCTTCAGTGc                                                                                                                >  2:9147865/1‑140 (MQ=255)

                            aCCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACa                                                                                                           <  1:1072410/140‑1 (MQ=255)

                             cccGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACag                                                                                                          >  1:3553351/1‑140 (MQ=255)

                                 aaaaCCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCt                                                                                                      >  2:6502523/1‑140 (MQ=255)

                                      caaccaCACACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACACTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGac                                                                                                 <  2:1845964/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTg                                                                                                     <  1:6694844/134‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                               <  2:5975312/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                               <  2:5826444/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                               >  2:3469712/1‑140 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                               <  1:4728180/140‑1 (MQ=255)

                                        accaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTGACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGacca                                                                                               <  2:4301874/140‑1 (MQ=255)

                                         ccaCAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGaccac                                                                                              <  2:73213/140‑1 (MQ=255)

                                              aaCACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                         <  1:2205695/140‑1 (MQ=255)

                                              aaCACGACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGAccaccacca                                                                                         <  1:8581077/140‑1 (MQ=255)

                                                  cTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacaca                                                                                     <  1:8420040/140‑1 (MQ=255)

                                                   tACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacac                                                                                    <  2:1083080/140‑1 (MQ=255)

                                                    aCCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGCACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATCATCTTCAGTGCAAACAGAGCTGCGACCACCACcacacaca                                                                                   >  2:2574308/1‑140 (MQ=255)

                                                       gCCAGCGAGGCGAGCATTATCCAGATCATGACTTCACCTGCGTAATCAGAACTCAAGCTATCAGGAAACCCTCGCCAGTTACTAATCCACCAGAAAAATCTACAGTGCAAACAGAGCTGCGACCCCCACCACACACAGGt                                                                                >  2:7056585/1‑140 (MQ=255)

                                                        cctgCGTTGCGAGCATTATCCAGTTCATTAGTTATCCTGCTTAATCAGAAATCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAATCAGAGCTGAGACCACCACCACACACAGGtt                                                                               <  1:530003/137‑1 (MQ=255)

                                                         cAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTc                                                                              >  1:7785294/1‑140 (MQ=255)

                                                                gCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATcgc                                                                       >  2:8432835/1‑140 (MQ=255)

                                                                 cGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCACGCCAGTTACTAATCCACCAGAATAATCATCAGTCCCACCAGAGCTGCGACCCCCACCACACACAGGATCAGATcgcg                                                                      >  2:2892479/1‑140 (MQ=255)

                                                                    gCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTACTCCACCAGAATACTCTTCAGTGCAAACAGAGCTGCGACCACCACCACCCACAGGTTCAGATCGCGCCa                                                                   <  2:780968/140‑1 (MQ=255)

                                                                        tATCCAGATCATGAGTACTCCTGCGTCATCAGAACTCATGATATCTGGAACCCCTCGCCAGTTACTAATCCCCCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAgcgc                                                               <  2:6561221/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                              <  1:1790241/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                              <  2:8065692/140‑1 (MQ=255)

                                                                         aTCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGcc                                                              <  1:3836552/140‑1 (MQ=255)

                                                                          tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAACCCCTCGCCAGTTACTAATCCACCCGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACCCACAGGTTCAGATCGCGCCAGCGccc                                                             <  2:8786517/140‑1 (MQ=255)

                                                                          tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGccc                                                             <  1:5020392/140‑1 (MQ=255)

                                                                          tCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAAGCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGccc                                                             >  2:8195842/1‑140 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                        >  1:9558080/1‑140 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGc                                                        >  1:6614388/1‑140 (MQ=255)

                                                                               aTCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACAAATCCACCAGAATAATCTAAAGTGCAAACAGAGCTGCGATCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTga                                                        >  2:6907951/1‑139 (MQ=255)

                                                                                tCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCa                                                       <  1:6528872/140‑1 (MQ=255)

                                                                                  aTGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATa                                                     >  2:7573616/1‑140 (MQ=255)

                                                                                   tGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                                    <  1:10931770/140‑1 (MQ=255)

                                                                                   tGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                                    <  1:2746118/140‑1 (MQ=255)

                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACt                                                   >  2:6679461/1‑140 (MQ=255)

                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACt                                                   >  2:8984311/1‑140 (MQ=255)

                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACt                                                   >  2:1136732/1‑140 (MQ=255)

                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACt                                                   >  2:3269358/1‑140 (MQ=255)

                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACt                                                   >  1:6381956/1‑140 (MQ=255)

                                                                                    gAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACt                                                   >  2:5265045/1‑140 (MQ=255)

                                                                                        tctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCa                                               <  1:5685959/140‑1 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                              >  2:2447169/1‑140 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                              >  1:3043965/1‑140 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                              >  1:4566499/1‑140 (MQ=255)

                                                                                         ctcCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACAAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCat                                              >  2:5525192/1‑140 (MQ=255)

                                                                                           ccTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatga                                            <  2:10054680/140‑1 (MQ=255)

                                                                                           ccTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatga                                            <  1:4457981/140‑1 (MQ=255)

                                                                                           ccTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatga                                            <  1:7515687/140‑1 (MQ=255)

                                                                                             tGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCatgatg                                          <  1:7245238/140‑1 (MQ=255)

                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATAc                                                    >  1:4176942/1‑126 (MQ=255)

                                                                                                 tAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCAACCAGAATAATCTTCAGTGCAAACAGAGCAGCGACCCCCACCACACACAGGTTCAGAACGCGCCAGCGCCCGGTGCATACTGTCATGATGCAga                                      >  2:10265813/1‑139 (MQ=255)

                                                                                                  aaTCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTa                                     <  1:1994065/140‑1 (MQ=255)

                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACg                                   <  2:9802245/140‑1 (MQ=255)

                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACg                                   >  2:6313859/1‑140 (MQ=255)

                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACg                                   >  2:7165494/1‑140 (MQ=255)

                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACg                                   >  2:8637977/1‑140 (MQ=255)

                                                                                                    tCAGAACTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACg                                   >  1:6013755/1‑140 (MQ=255)

                                                                                                        aaCTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACAt                               <  1:10363967/140‑1 (MQ=255)

                                                                                                         aCTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACATAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATa                              <  2:3602572/140‑1 (MQ=255)

                                                                                                         aCTCATGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGTCATa                              <  2:4852414/140‑1 (MQ=255)

                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGc                          >  2:9666416/1‑140 (MQ=255)

                                                                                                             aTGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGc                          >  2:10676252/1‑140 (MQ=255)

                                                                                                              tGATATCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGcc                         <  1:6142889/140‑1 (MQ=255)

                                                                                                                  atCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGt                     >  2:4547259/1‑140 (MQ=255)

                                                                                                                  atCTGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGt                     >  1:3203262/1‑140 (MQ=255)

                                                                                                                     tGGAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCa                  <  1:8738087/140‑1 (MQ=255)

                                                                                                                      ggAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCaa                 <  1:7165494/140‑1 (MQ=255)

                                                                                                                       gAAACCCTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAAt                >  2:4132618/1‑140 (MQ=255)

                                                                                                                           cccTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCt            >  2:3129376/1‑140 (MQ=255)

                                                                                                                             cTCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTc          >  2:3962412/1‑140 (MQ=255)

                                                                                                                              tCGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCg         <  1:1220134/140‑1 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCgg        >  1:3354529/1‑140 (MQ=255)

                                                                                                                               cGCCAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACAT‑AAGCCGAGTGCAATCCCTTCGGt       >  1:1484020/1‑140 (MQ=255)

                                                                                                                                 ccAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTg      >  1:9745961/1‑140 (MQ=255)

                                                                                                                                 ccAGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTg      >  2:9455541/1‑140 (MQ=255)

                                                                                                                                   aGTTACTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGAt    <  2:2335596/140‑1 (MQ=255)

                                                                                                                                     ttactaATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGATCg  <  1:7975277/140‑1 (MQ=255)

                                                                                                                                     ttactaATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGATCg  <  2:2679839/140‑1 (MQ=255)

AATCGCTTTTCTGGCCCCGGATGTCAGCACCCGAAAACCAACCACAAACACTACCGCCAGCGTGGCGAGCATTATCCAGATCATGAGTTCTCCTGCGTAATCAGAACTCATGATATCTGGAAACCCTCGCCAGTTATTAATCCACCAGAATAATCTTCAGTGCAAACAGAGCTGCGACCACCACCACACACAGGTTCAGATCGCGCCAGCGCCCGGTGCATACTTTCATGATGCAGTACGACATAAAGCCGAGTGCAATCCCTTCGGTGATCG  >  NZ_CP025268/3844242‑3844514

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`