BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,449,815	A→G	pseudogene (3779/3779 nt)	tamB →	autotransporter assembly complex protein TamB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,449,815	0	A	G	99.9%	2919.6 / NA	840	pseudogene (3779/3779 nt)	tamB	autotransporter assembly complex protein TamB
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (463/376); minor base T (1/0); total (464/376)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 840 total aligned reads displayed.

CAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAG  >  NZ_CP025268/4449679‑4449950

  aGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTACGCTATATCTGGAAGCCGTGTCTGGTGTAGTCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAAt                                                                                                                                     <  2:4480179/140‑1 (MQ=255)

          aCGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAatat                                                                                                                             <  2:3883836/140‑1 (MQ=255)

          aCGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAatat                                                                                                                             <  2:8929987/140‑1 (MQ=255)

            ggCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATAttt                                                                                                                           <  1:7191088/140‑1 (MQ=255)

                tGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCt                                                                                                                       >  2:5936494/1‑140 (MQ=255)

                  ggTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTAc                                                                                                                     >  1:1425334/1‑140 (MQ=255)

                    tataTTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACgg                                                                                                                   <  1:8493633/140‑1 (MQ=255)

                        tttGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGt                                                                                                               >  1:2629285/1‑140 (MQ=255)

                          tGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGttt                                                                                                             <  1:879309/140‑1 (MQ=255)

                          tGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGttt                                                                                                             <  2:10590422/140‑1 (MQ=255)

                            aCTCTATATCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                                           >  2:1891629/1‑140 (MQ=255)

                            aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                                           >  2:10048956/1‑140 (MQ=255)

                            aCTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTAc                                                                                                           >  1:10611984/1‑140 (MQ=255)

                             ctctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                          >  1:9819030/1‑140 (MQ=255)

                             ctctATAGCAACACTCACGTTACGTTATAGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACg                                                                                                          >  2:9234987/1‑140 (MQ=255)

                              tctATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGc                                                                                                         >  1:8689871/1‑140 (MQ=255)

                                  taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                     <  1:4967164/140‑1 (MQ=255)

                                  taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                     <  1:552798/140‑1 (MQ=255)

                                  taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                     >  2:1292214/1‑140 (MQ=255)

                                  taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                     >  1:7968874/1‑140 (MQ=255)

                                  taGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCcaca                                                                                                     <  1:5942001/140‑1 (MQ=255)

                                        cacTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCACTGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAg                                                                                               >  2:2552192/1‑140 (MQ=255)

                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                             >  1:8064298/1‑140 (MQ=255)

                                          cTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGc                                                                                             >  1:6560108/1‑140 (MQ=255)

                                            cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                           >  1:9445445/1‑140 (MQ=255)

                                            cACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                           <  1:3795095/140‑1 (MQ=255)

                                              cgttacgttaTCGCCAGATGCCTAAGCTAGATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑TGTGCTCTATCAGTTCGAGTTTTCGCAATGCGAATATTGGTCGCCGGCAGTTTACGCCACAAACAAGGCAACa                                                                                         <  1:1105906/140‑1 (MQ=255)

                                                  acgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCa                                                                                     >  2:377503/1‑140 (MQ=255)

                                                   cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                           >  1:4636825/1‑133 (MQ=255)

                                                   cgttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCAc                                                                                    <  1:6718206/140‑1 (MQ=255)

                                                    gttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACt                                                                                   <  2:7530823/140‑1 (MQ=255)

                                                     ttaTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTg                                                                                  >  2:1665380/1‑140 (MQ=255)

                                                      taTCGCCTGATGCCTCAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTgg                                                                                 <  2:10759218/140‑1 (MQ=255)

                                                       aTCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGATTATTTGTCTACGGCAGTTTACGCCACAAACGAGGCAACAGTCACTggg                                                                                >  1:9270011/1‑139 (MQ=255)

                                                        tCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                                               <  2:8021598/140‑1 (MQ=255)

                                                        tCGCCTGAAGCCTAAGCTATATCGGGCAGCAGTGTCTGGTGTAGACCAGGCACTGG‑A‑TGTGCTCTAACCCTTCGAGTTTGAGCAAGGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGAt                                                                               <  2:2199722/140‑1 (MQ=255)

                                                         cGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATg                                                                              >  2:4723708/1‑140 (MQ=255)

                                                           ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                                            >  2:10844423/1‑140 (MQ=255)

                                                           ccTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGAc                                                                            >  1:3669170/1‑140 (MQ=255)

                                                             tGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCTAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCa                                                                          <  2:3492325/140‑1 (MQ=255)

                                                              gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTTG‑ATT‑TGCTATATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                         <  1:2080883/140‑1 (MQ=255)

                                                              gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                         >  1:4013417/1‑140 (MQ=255)

                                                              gATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                         >  1:1311551/1‑140 (MQ=255)

                                                              gATGCCTAAGCTATATCTGGAAGACTTGTCTGGTGTAGACCAGGCACTTG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCaa                                                                         <  2:6956022/140‑1 (MQ=255)

                                                               aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                                                        >  1:8333983/1‑140 (MQ=255)

                                                               aTGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAAt                                                                        >  2:8694020/1‑140 (MQ=255)

                                                                tGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTGCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGTCAACAGTCACTGGATGACCAATg                                                                       >  2:7918954/1‑140 (MQ=255)

                                                                  ccTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGcc                                                                     <  2:10180266/140‑1 (MQ=255)

                                                                      aGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGt                                                                 >  2:3256124/1‑140 (MQ=255)

                                                                         tataTCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTAc                                                              <  1:8245880/140‑1 (MQ=255)

                                                                            atCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTgg                                                           >  2:5316845/1‑140 (MQ=255)

                                                                             tCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTggg                                                          >  1:9045914/1‑140 (MQ=255)

                                                                              cTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGc                                                         <  1:9618516/140‑1 (MQ=255)

                                                                                ggAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGa                                                       >  1:10005560/1‑140 (MQ=255)

                                                                                ggAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGa                                                       >  1:7000853/1‑140 (MQ=255)

                                                                                     ccGTGTCTGGTGTTGACCAGGCACTGG‑A‑TGTGCTCGAGCAGTTCGCGTTTTAGCCTTGCGTTTATGTGTCTTCGGCAGTTGGCGCCACAAACAAGGCAACAGTCACTGGATGACCATTGCCCAGTGACTGGGCGTTTTcg                                                  >  2:4124700/1‑139 (MQ=255)

                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                                  >  2:954230/1‑140 (MQ=255)

                                                                                     ccGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCa                                                  <  1:2329139/140‑1 (MQ=255)

                                                                                      cGTGTCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAg                                                 >  1:5320780/1‑140 (MQ=255)

                                                                                        tgtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTCGCACTGCGAATATGTGTCTACGGCAGTTTACGCCACACACACGGCAACAGTCACTGGATGACCCATGCCCAGTGACTGGGCGATTGCAGTa                                               >  2:10296899/1‑140 (MQ=255)

                                                                                        tgtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                                               >  1:5114082/1‑140 (MQ=255)

                                                                                        tgtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                                               <  1:4822529/140‑1 (MQ=255)

                                                                                        tgtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTa                                               >  2:4287944/1‑140 (MQ=255)

                                                                                         gtCTGGTGTAGACCAGGCACTGGAA‑T‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACCAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                                              <  2:2785616/140‑1 (MQ=255)

                                                                                         gtCTGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTAt                                              <  1:8914015/140‑1 (MQ=255)

                                                                                            tGGTGTAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGa                                           >  1:7308622/1‑140 (MQ=255)

                                                                                              gtgtAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATa                                         <  2:980593/140‑1 (MQ=255)

                                                                                                 tAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                      <  1:3405684/140‑1 (MQ=255)

                                                                                                 tAGACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACt                                      <  1:1652363/140‑1 (MQ=255)

                                                                                                   gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                    <  1:7608532/140‑1 (MQ=255)

                                                                                                   gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                    <  1:6232878/140‑1 (MQ=255)

                                                                                                   gACCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                    >  2:706926/1‑140 (MQ=255)

                                                                                                    aCCAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAcc                                   <  2:733228/140‑1 (MQ=255)

                                                                                                     ccAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                  <  1:10327440/140‑1 (MQ=255)

                                                                                                     ccAGGCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCa                                  <  1:3108265/140‑1 (MQ=255)

                                                                                                        ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTAc                                    <  1:1954862/135‑1 (MQ=255)

                                                                                                        ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt                               >  1:5789694/1‑140 (MQ=255)

                                                                                                        ggCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGtt                               >  1:4340071/1‑140 (MQ=255)

                                                                                                         gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                              >  2:7759111/1‑140 (MQ=255)

                                                                                                         gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                              >  2:2986531/1‑140 (MQ=255)

                                                                                                         gCACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                              >  2:6524692/1‑140 (MQ=255)

                                                                                                         gCACTGG‑A‑TGTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTg                              >  1:10231625/1‑140 (MQ=255)

                                                                                                          cACTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGt                             <  1:6857753/140‑1 (MQ=255)

                                                                                                           aCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                            <  2:8548289/140‑1 (MQ=255)

                                                                                                           aCTGG‑ATT‑TGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATGTGTCTACGGCAGTTAACGCCACAACCAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                            <  2:8296663/140‑1 (MQ=255)

                                                                                                           aCTGG‑A‑TGTGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGta                            <  2:6309119/140‑1 (MQ=255)

                                                                                                                   t‑tGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACCGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTg                     <  1:189469/140‑1 (MQ=255)

                                                                                                                     tGCTCTATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCaa                                                                                           <  2:5453192/69‑1 (MQ=255)

                                                                                                                         ctATCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCa                <  2:5812723/140‑1 (MQ=255)

                                                                                                                           aTCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACt              >  1:10741871/1‑140 (MQ=255)

                                                                                                                           aTCAGTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTATACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACt              >  2:9027490/1‑140 (MQ=255)

                                                                                                                               gTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACACACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATcc          <  2:6753765/140‑1 (MQ=255)

                                                                                                                               gTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATcc          <  2:6153546/140‑1 (MQ=255)

                                                                                                                               gTTCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATcc          <  2:2000273/140‑1 (MQ=255)

                                                                                                                                ttCGTGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGGTGTATAGCCTGGGCCACTAGccc         >  2:4822569/1‑139 (MQ=255)

                                                                                                                                ttCGAGTATTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCACCAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCa         <  2:728409/140‑1 (MQ=255)

                                                                                                                                 tCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAg        >  1:2479260/1‑140 (MQ=255)

                                                                                                                                 tCGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAg        >  2:5066606/1‑140 (MQ=255)

                                                                                                                                  cGAGTTTTAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAgg       <  1:1564570/140‑1 (MQ=255)

                                                                                                                                      ttttAGCAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCa   >  1:6403128/1‑140 (MQ=255)

CAAGTGAAATACGGCGTGGGTATATTTGACTCTATAGCAACACTCACGTTACGTTATCGCCTGATGCCTAAGCTATATCTGGAAGCCGTGTCTGGTGTAGACCAGGCACTGG‑A‑T‑TGCTCTATCAGTTCGAGTTTTAACAATGCGAATATTTGTCTACGGCAGTTTACGCCACAAACAAGGCAACAGTCACTGGATGACCAATGCCCAGTTACTGGGCGATTTCAGTATCGATAACTACCAGTTGTATAGCCTGGGCCACTATCCAGGCGCAG  >  NZ_CP025268/4449679‑4449950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`