BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	4,621,820	G→A	G104E (GGA→GAA)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	4,621,820	0	G	A	99.2%	2375.2 / NA	628	G104E (GGA→GAA)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (289/333); minor base C (4/1); total (294/334)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 641 total aligned reads displayed.

TCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGT  >  NZ_CP025268/4621683‑4621956

tCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGaaa                                                                                                                                        >  1:6364767/1‑140 (MQ=255)

    gggCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGc                                                                                                                                    <  2:10235662/140‑1 (MQ=255)

    gggCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGc                                                                                                                                    <  1:8589419/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  2:9436882/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  2:3434634/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  2:1212066/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  1:8889118/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  1:1775200/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  1:2343850/140‑1 (MQ=255)

       cTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 <  1:410976/140‑1 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCgg                                                                                                                                 >  2:1669912/1‑137 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                              >  1:10372177/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                              >  1:8135348/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                              >  1:8074089/1‑140 (MQ=255)

          gCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGgtgt                                                                                                                              >  1:3223924/1‑140 (MQ=255)

           cGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTc                                                                                                                             <  2:6597057/140‑1 (MQ=255)

             aaaGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTccc                                                                                                                           <  1:908312/140‑1 (MQ=255)

               aGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCgg                                                                                                                         <  1:7118026/140‑1 (MQ=255)

               aGCACACGAAGGTTCTACCGGTTTCAGTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGAGCGGACGCAGGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAAGTGCCGGTGTCCCgg                                                                                                                         <  2:5951387/140‑1 (MQ=255)

                 cacaCGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGtt                                                                                                                       <  1:5267398/140‑1 (MQ=255)

                    acGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTg                                                                                                                    >  1:284750/1‑140 (MQ=255)

                     cGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGt                                                                                                                   >  2:3716271/1‑140 (MQ=255)

                       aaGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGttt                                                                                                                 <  1:9759356/140‑1 (MQ=255)

                         ggTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTGTGa                                                                                                               >  2:5439289/1‑140 (MQ=255)

                          gaacaaCCGGTTACATTCCGGCGGGAATGGACGGCAACGCTGAAGTAAACGGCGCGTACGCATGTGCGCACGAAATGTCATCCGGTAAACATACCCCGTCTGGTAACTGGGAAAATGCCGCTGTCCCGGATCTGTTTGAg                                                                                                              >  2:7400678/6‑140 (MQ=255)

                          gTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAg                                                                                                              >  2:6080249/1‑140 (MQ=255)

                           ttCGACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGt                                                                                                             <  1:961677/140‑1 (MQ=255)

                             cTACCGGTTTCATTCCGGCGGGAATTGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                           <  1:10574405/140‑1 (MQ=255)

                             cTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGTAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTgg                                                                                                           <  1:262003/140‑1 (MQ=255)

                                ccGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGa                                                                                                        <  1:3328618/140‑1 (MQ=255)

                                ccGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGa                                                                                                        >  2:7561483/1‑140 (MQ=255)

                                  ggTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGata                                                                                                      >  1:1440523/1‑140 (MQ=255)

                                    tttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATAtt                                                                                                    >  2:5806879/1‑140 (MQ=255)

                                    tttCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATAtt                                                                                                    >  2:815081/1‑140 (MQ=255)

                                              gcggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATCCCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCa                                                                                          >  2:3035123/1‑140 (MQ=255)

                                              gcggGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGc                                                                                                                                    >  2:4185965/1‑98 (MQ=255)

                                                 ggAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGa                                                                                       <  2:355682/140‑1 (MQ=255)

                                                       gACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAg                                                                                 <  2:5002672/140‑1 (MQ=255)

                                                          ggCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCtt                                                                              <  2:1455650/140‑1 (MQ=255)

                                                          ggCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCtt                                                                              <  2:8646416/140‑1 (MQ=255)

                                                           gCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTc                                                                             >  2:270788/1‑140 (MQ=255)

                                                               cGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGc                                                                         >  1:5585649/1‑140 (MQ=255)

                                                                 cTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCaa                                                                       >  2:1309470/1‑140 (MQ=255)

                                                                 cTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGGCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCaa                                                                       >  1:6892064/1‑140 (MQ=255)

                                                                  tGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGATGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAag                                                                      <  1:7267356/140‑1 (MQ=255)

                                                                      gTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCTCTGGGAAATTGACGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCTCAagagat                                                                  <  2:6364767/140‑3 (MQ=255)

                                                                      gTTATCGGCGAGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGTGTGGGGATATTTCTCCGATCACGAAAACATCTTCCCGCAAGAgct                                                                  <  2:8393448/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  1:2478579/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  1:7794117/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  2:8286241/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  1:9942523/140‑1 (MQ=255)

                                                                       ttATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctg                                                                 <  1:1647089/140‑1 (MQ=255)

                                                                         aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTACAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctgct                                                               >  2:2158195/1‑140 (MQ=255)

                                                                         aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctgct                                                               >  2:10361975/1‑140 (MQ=255)

                                                                         aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctgct                                                               >  1:671486/1‑140 (MQ=255)

                                                                         aTCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAgctgct                                                               >  1:1333572/1‑140 (MQ=255)

                                                                            ggCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGa                                                            <  1:7503679/140‑1 (MQ=255)

                                                                            ggCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGa                                                            >  2:10626879/1‑140 (MQ=255)

                                                                            ggCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGa                                                            <  1:4268016/140‑1 (MQ=255)

                                                                                cgTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATaaa                                                        <  2:5330104/140‑1 (MQ=255)

                                                                                        tGGGCGCCCGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAACTTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGAGATGTCTCCGATCACGAAAACAGCTTCCCGCAAGTGCTGCTGGATAAACTGGTCGa                                                >  1:1083987/1‑140 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                <  2:9164466/140‑1 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                <  1:8237271/140‑1 (MQ=255)

                                                                                        tGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGa                                                <  2:9008766/140‑1 (MQ=255)

                                                                                         gggCGCACGAAATGACATCCGGAAACGATCCCCCGTCTGGTCACTGGGAAATTGACGGTGTCCCGGTTCTGATTGAGAGGCGATATTTCTCCGATCACCAAAACAGCTTCCCGCACGAGCTGCTGGATAAACTGGTCGaa                                               <  2:9708687/140‑1 (MQ=255)

                                                                                           gcgcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAAcg                                             <  1:8408365/140‑1 (MQ=255)

                                                                                             gcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAAcgcg                                           >  1:9167114/1‑140 (MQ=255)

                                                                                             gcACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAAcgcg                                           <  2:7189811/140‑1 (MQ=255)

                                                                                              cACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACgcgc                                          >  2:3988022/1‑140 (MQ=255)

                                                                                               aCGAAATGTCATCNGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGNTGCTGGATAAACTGGTCGAACGCGCt                                         >  1:5852635/1‑140 (MQ=255)

                                                                                               aCGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGTAATTGCCGGTGTCCCGGTTCTGGTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCt                                         >  2:3293201/1‑140 (MQ=255)

                                                                                               aCGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACAGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCt                                         >  1:8820052/1‑140 (MQ=255)

                                                                                                     tGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTg                                   >  2:10788762/1‑140 (MQ=255)

                                                                                                         aTCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCgg                               >  1:3552049/1‑140 (MQ=255)

                                                                                                          tCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCggg                              <  2:7795080/140‑1 (MQ=255)

                                                                                                          tCCGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCggg                              <  2:4868225/140‑1 (MQ=255)

                                                                                                           ccGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGt                             <  1:3661950/140‑1 (MQ=255)

                                                                                                            cGGTAAAGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGtt                            <  2:2858670/140‑1 (MQ=255)

                                                                                                                 aaGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTc                       >  1:207173/1‑140 (MQ=255)

                                                                                                                  aGATACCCCGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCg                      <  2:2548091/140‑1 (MQ=255)

                                                                                                                     tACCCCGTCTGGTCACTGGGAAAGTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCGTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTACTCTGCCGGGTTACCTCGGTa                   <  2:4694258/140‑1 (MQ=255)

                                                                                                                       ccccGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAAc                 >  1:1231210/1‑140 (MQ=255)

                                                                                                                        cccGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACt                >  2:647533/1‑140 (MQ=255)

                                                                                                                         ccGTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTg               >  1:4835573/1‑140 (MQ=255)

                                                                                                                           gTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATNTTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGcc             >  1:10132361/1‑140 (MQ=255)

                                                                                                                           gTCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGcc             >  1:4715811/1‑140 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAACTTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            <  2:6746113/140‑1 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            <  2:2432203/140‑1 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            <  1:291147/140‑1 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            <  1:7732186/140‑1 (MQ=255)

                                                                                                                            tCTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCa            >  1:4438157/1‑140 (MQ=255)

                                                                                                                             cTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTCCCTCGGTAACTGCCAc           >  2:7885760/1‑140 (MQ=255)

                                                                                                                             cTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCAc           <  1:3833407/140‑1 (MQ=255)

                                                                                                                             cTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCAc           >  1:9435876/1‑140 (MQ=255)

                                                                                                                             cTGGTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCAc           <  1:6134243/140‑1 (MQ=255)

                                                                                                                              tGGTCACTGGGAATTTGCCGGTGTCCCGGTGCTGGTTGTGTGGGGATATGTCTCCGCTCACGAAATCAGCTTCCCGCAAGAGCTGCTGGGTAAACTGGTCGTACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCAct          >  2:1845474/1‑140 (MQ=255)

                                                                                                                               ggTCACTGGGAAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCActc         >  2:6277379/1‑140 (MQ=255)

                                                                                                                                     tGGGAAAGTGCCGGTGTCCCGTTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTTGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCgg   <  2:4363837/140‑1 (MQ=255)

                                                                                                                                      gggaAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGt  >  2:8803255/1‑140 (MQ=255)

                                                                                                                                      gggaAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGt  >  2:2294983/1‑140 (MQ=255)

TCTGGGGCTGGCGAAAGCACACGAAGGTTCTACCGGTTTCATTCCGGCGGGAATGGACGGCAACGCTGAAGTTATCGGCGCGTACGCATGGGCGCACGAAATGTCATCCGGTAAAGATACCCCGTCTGGTCACTGGGGAATTGCCGGTGTCCCGGTTCTGTTTGAGTGGGGATATTTCTCCGATCACGAAAACAGCTTCCCGCAAGAGCTGCTGGATAAACTGGTCGAACGCGCTAATCTGCCGGGTTACCTCGGTAACTGCCACTCTTCCGGT  >  NZ_CP025268/4621683‑4621956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`