BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,292,822	2 bp→GG	noncoding (36‑37/132 nt)	CXP41_RS06830 ←	RtT sRNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,292,822	0	A	G	91.3%	627.1 / 68.5	380	noncoding (37/132 nt)	CXP41_RS06830	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (17/15); new base G (70/265); total (96/284)
Fisher's exact test for biased strand distribution p-value = 2.62e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	1,292,823	0	A	G	99.7%	819.0 / NA	380	noncoding (36/132 nt)	CXP41_RS06830	RtT sRNA
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (96/283); minor base C (0/1); total (96/284)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 380 total aligned reads displayed.

TAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCG  >  NZ_CP025268/1292688‑1292955

||

tAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGa                                                                                                                                  <  1:7717365/140‑1 (MQ=255)

tAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGa                                                                                                                                  <  2:1930131/140‑1 (MQ=255)

tAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGa                                                                                                                                  <  1:4437773/140‑1 (MQ=255)

 aGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGaa                                                                                                                                 <  2:1725591/140‑1 (MQ=255)

 aGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGaa                                                                                                                                 <  2:3220311/140‑1 (MQ=255)

 aGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGaa                                                                                                                                 <  1:2695004/140‑1 (MQ=255)

 aGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGaa                                                                                                                                 <  1:8912970/140‑1 (MQ=255)

  gCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAg                                                                                                                                <  1:2005604/140‑1 (MQ=255)

  gCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAg                                                                                                                                <  2:7866737/140‑1 (MQ=255)

         gTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTAGAAGCTTCTCa                                                                                                                         >  1:10275515/1‑140 (MQ=17)

          tAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                        <  2:3685745/140‑1 (MQ=255)

          tAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                        <  2:7002411/140‑1 (MQ=255)

          ataCAGCTTTTGTTTAATTAAGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAt                                                                                                                        <  2:5803538/138‑1 (MQ=11)

               ggTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTc                                                                                                                   <  1:2502470/140‑1 (MQ=255)

                  tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcccc                                                                                                                >  1:9170683/1‑140 (MQ=255)

                  tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCAGGCGCTCAAGTCGAAACTTGGGAGGAGCTTCTCATCCTTcccc                                                                                                                >  1:185891/1‑140 (MQ=14)

                  tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCATGGTCGAAGCTTATCATCCTTcccc                                                                                                                >  1:9445261/1‑140 (MQ=14)

                  tttGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCATCGGGTCGTTGCCTGCGGCAACCCACACACGCTGGCGCTCAACTCGAAACTTGGTCGAACCTTATCATCCTTcccc                                                                                                                >  1:6577311/1‑140 (MQ=14)

                    tGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                              <  1:1380998/140‑1 (MQ=255)

                    tGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc                                                                                                              <  1:2434625/140‑1 (MQ=255)

                    tGATTATTGAAGGACGCGATGGTGGTGGGGGACGGATTCCTCATCGCTGCGCGCTTCGCCCTTCGGGACGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCAGCCTTCCCCGc                                                                                                              <  2:4417390/140‑1 (MQ=14)

                        tattTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATgg                                                                                                          >  1:9841231/1‑140 (MQ=255)

                         attTTAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATggg                                                                                                         <  2:2987315/140‑1 (MQ=255)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                         <  2:1014800/140‑1 (MQ=14)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                         <  1:7061171/140‑1 (MQ=14)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                         <  1:5221306/140‑1 (MQ=14)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                         <  1:4169420/140‑1 (MQ=14)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                         <  2:4650832/140‑1 (MQ=14)

                         attTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTggg                                                                                                         <  2:8141346/140‑1 (MQ=14)

                          tttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTTGTCGAAGCTTCTCAACCTTCCCCGCCTGGGc                                                                                                        >  2:5881676/1‑140 (MQ=11)

                           ttAAGGAAGCGATGGTGGTGGGGGAAGTATTACTCAGCTCTGCGCGCTTCGCCCTTCGGGTCGTTTCATTCGGCAACGCTATAACGCTGGCGCTCAAGTCGAACCATGGTCGAAGCTTAACATCATTCCCCGCATGGTCa                                                                                                       >  1:663706/1‑140 (MQ=11)

                           ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGACGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                       >  2:6850186/1‑140 (MQ=11)

                           ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                       <  1:3467726/140‑1 (MQ=11)

                           ttAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGAGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTACCCGCTTGGGCa                                                                                                       <  2:9890454/140‑1 (MQ=11)

                           tcacgtaaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGAGCTTCGCCCTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCACAAGTCGAACCATGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCa                                                                                                       <  1:688601/134‑1 (MQ=11)

                             aaGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGa                                                                                                     <  1:4830552/140‑1 (MQ=11)

                                    cGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTg                                                                                              >  2:6402849/1‑140 (MQ=11)

                                    cGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACCCGCACGAGCTGGCGCTCAATTAGAAACGTAGTCGAAGCTTCACATACTTCCCCGCAGGGGCAGGCTATTTg                                                                                              >  1:75465/1‑140 (MQ=2)

                                                           cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGACAGCGGCAACGCTCTCTCGCTGGCGCTAAAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTGTGAGAATTc                                                                       >  2:8402299/1‑140 (MQ=11)

                                                             cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCg                                                                     <  1:10087192/140‑1 (MQ=11)

                                                               gcgcggcgcgCGTCGCCCTTCGGGTCGGGGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGACGCTTCTCATCCGTCCCCGCTGGGGCAGAATATTTGATTGCGGATTCGTGTGAGAAGTCCggg                                                                   <  2:8585051/140‑1 (MQ=11)

                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCggg                                                                   <  2:4804941/140‑1 (MQ=11)

                                                               gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCggg                                                                   <  2:6354125/140‑1 (MQ=11)

                                                                cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCgggg                                                                  <  2:8485755/140‑1 (MQ=11)

                                                                   tGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACACTAGCTGGCGCACAAGTCGAAACGTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGGTTGAGAATTCCGGGGGtt                                                               >  1:7358042/1‑140 (MQ=11)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCtttt                                                             <  2:4177958/140‑1 (MQ=11)

                                                                     cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCAAGTCGAACCTTGGTCGAAGCTTCTCATCCGTCCCCCCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCtttt                                                             >  1:6449607/1‑140 (MQ=11)

                                                                      gcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTg                                                            <  2:5281413/140‑1 (MQ=11)

                                                                        gcTTCGCCCGTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGaa                                                          <  2:4074024/140‑1 (MQ=11)

                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGaaa                                                         <  2:10387982/140‑1 (MQ=11)

                                                                         cTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGaaa                                                         <  2:5577636/140‑1 (MQ=11)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATCTTTGATTGCGGATTCGGGTGAGAATTCAGGGGCGGTGGAAAGTGa                                                     >  2:6605742/1‑140 (MQ=11)

                                                                             gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGa                                                     <  1:7498424/140‑1 (MQ=11)

                                                                                cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtgg                                                  <  1:7865877/140‑1 (MQ=11)

                                                                                cTTCGGGGCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCGTGGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtgg                                                  <  1:2217507/140‑1 (MQ=11)

                                                                                   cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                               <  1:9512134/140‑1 (MQ=11)

                                                                                   cGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGAtggtgg                                               <  1:8594715/140‑1 (MQ=11)

                                                                                       tCGTTGCCTGCGGCAACGCTCTCGCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggg                                           <  2:2649498/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGNGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:5890377/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  2:1178163/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  2:1417158/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  2:10628809/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:5582851/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:4659132/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  2:7515181/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:6272067/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  2:7822350/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:996450/140‑1 (MQ=11)

                                                                                         gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTggggg                                         <  1:8263299/140‑1 (MQ=11)

                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGATTTCCGGGTCTTTTGAAAGTGAtggtggt                                              <  1:10421289/133‑1 (MQ=11)

                                                                                           tGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGACGAACCTTCGTCGAAGCATCAAATCCTTCCCCGCATGGGCAGCAGCTTTGGTTGCGGATTAGGTTGAGATGTCCGGGGCTGTTGAAAGTGATGggggggggggtc                                       >  2:2184933/1‑138 (MQ=2)

                                                                                              cTGCGTCATCGCTCTCTCGCTGGCTCTCACGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGAGTCGTTTGAGAATTCCGGGGCTTTTGAACGTGATGGTGGTGGGGGAAGGa                                    <  2:6726557/140‑1 (MQ=11)

                                                                                              cTGCGGCTACGCTCTCTCGCTGGCGCTCACGTCGAACCTTGGTCGAAGCTTCTCCTCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  2:3891426/140‑1 (MQ=11)

                                                                                              cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGCACCATGGTCGAAGCTTCTCCTCCTTCCCCGCGTGGGCAGAATATTTGATTGCGGATACGTTGTAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  2:3600097/140‑1 (MQ=11)

                                                                                              cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCTCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  2:8275232/140‑1 (MQ=11)

                                                                                              cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  2:4744731/140‑1 (MQ=11)

                                                                                              cTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAAACTTGGTCGAAGCTTCTCATCCGTCCCCGCTTGGGCAGAATAATTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGa                                    <  2:240300/140‑1 (MQ=11)

                                                                                              cTGCCGCAACGCTCGCTCGCTGGCGCCCACGTCGAACCCTGCTCGAAGCTTCTCCTCCGTCCCCGCTTGGGTAGAATATTGGATTGCCGATTCGTATGAGAATTCCGGGGCTTTTGAATGTGATGGTGTTGGGGGattta                                    <  2:7681522/140‑5 (MQ=255)

                                                                                                   gCAACGCTCTCTCGATGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                               <  1:1290404/140‑1 (MQ=14)

                                                                                                           ctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTg                       <  1:5335002/140‑1 (MQ=14)

                                                                                                           ctctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTg                       <  2:10275515/140‑1 (MQ=14)

                                                                                                            tctcGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                      <  2:8905704/140‑1 (MQ=14)

                                                                                                            tctcGCTGACGCTCGAGCCGAAACTTAGTCGTAGCTTCTCATCCGTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgc                      <  2:6041477/140‑1 (MQ=11)

                                                                                                                  tGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt                >  1:6796745/1‑140 (MQ=14)

                                                                                                                   ggCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGa                                        <  1:3532884/115‑1 (MQ=14)

                                                                                                                    gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg              >  1:4623895/1‑140 (MQ=14)

                                                                                                                    gcgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCg              <  1:3974195/140‑1 (MQ=14)

                                                                                                                    acgcTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt                 <  2:7740572/136‑1 (MQ=255)

                                                                                                                     cgcTCGAGCCGAACCTTGGTCGAAGCTTCTCAGCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGc             <  2:7950244/140‑1 (MQ=11)

                                                                                                                     cgcTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGc             <  1:3642003/140‑1 (MQ=14)

                                                                                                                        tcaagtcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt          <  2:663706/134‑1 (MQ=14)

                                                                                                                        tcaagtcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCt          <  1:5441027/134‑1 (MQ=14)

                                                                                                                          gAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAGTTCAGGGTCTTTTGAAAGTTTTGGTGGTGGGGGTAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc        <  2:7358042/140‑1 (MQ=11)

                                                                                                                          gAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc        <  1:10024510/140‑1 (MQ=255)

                                                                                                                            gCCGAACCTTGGTCGAAGCTTCGGATCCGTCCCCGCTTGGGCAGAATTTTTGATTGCGGATTCGCTTGAGTGTTGAGGGACTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgg      <  2:9460568/140‑1 (MQ=11)

                                                                                                                             tcGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCggg     <  1:3102590/139‑1 (MQ=255)

                                                                                                                              cGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCg                                                                     >  1:10567998/1‑75 (MQ=11)

                                                                                                                              cGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCg                                                                     <  2:10567998/75‑1 (MQ=11)

                                                                                                                                aaCCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTTATTGCGGATACGTTTGAGAATTCCGGGGCTTTTGACAGAGATGGTGGTGGGGGACGGCTTACTAGGAGCTGAGCGCTTCGCCCTTCGGGGCg  >  2:10040562/1‑140 (MQ=14)

                                                                                                                                aaCCTTAGTCGAAGCTTAACATCATTCCCCGCATGGGCATAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTAAGCGCTGCGAGCTTCGCCCTTCGGGTCg  >  1:7277448/1‑140 (MQ=11)

||

TAGCTCGGAGTAACAGGTTTTGATTATTTAAGGAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAATCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCG  >  NZ_CP025268/1292688‑1292955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`