Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,728,808 (C)10→8 pseudogene (1254‑1255/1457 nt) xylB ← xylulokinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,728,8000C.89.9% 449.3 / 45.4 120pseudogene (1263/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (10/2);  new base . (56/51);  total (67/53)
Fisher's exact test for biased strand distribution p-value = 6.35e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NZ_CP0252683,728,8010C.90.7% 371.1 / 52.0 120pseudogene (1262/1457 nt)xylBxylulokinase
Reads supporting (aligned to +/- strand):  ref base C (2/2);  major base . (49/49);  minor base G (9/1);  total (67/53)
Fisher's exact test for biased strand distribution p-value = 1.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 139 total aligned reads displayed.

GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728661‑3728932
                                                                                                                                           ||                                                                                                                                   
ggCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACACTTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      <  2:8948638/140‑1 (MQ=255)
  cTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                    <  1:3918739/140‑1 (MQ=255)
      tAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccc                                                                                                                                <  2:5166495/140‑1 (MQ=255)
        aGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccg                                                                                                                              <  1:3412766/140‑2 (MQ=255)
         gCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACGGccccccccgg                                                                                                                             >  2:10784941/1‑138 (MQ=255)
          cGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATcccgccccgga                                                                                                                            >  1:7191260/1‑137 (MQ=255)
              aTAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccccccccgtacggt                                                                                                                        <  1:2716955/140‑8 (MQ=255)
               tAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTa                                                                                                                     >  1:3732922/1‑140 (MQ=255)
                  cGCTGCGCATCTTGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGTAATc                                                                                                                  >  2:8953995/1‑140 (MQ=255)
                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAg                                                                                                               >  2:9347844/1‑140 (MQ=255)
                     tGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGGAATCGAg                                                                                                               >  1:3930360/1‑140 (MQ=255)
                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                    <  2:11212269/119‑1 (MQ=255)
                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                    >  1:11212269/1‑119 (MQ=255)
                       cgcATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATccc                                                                                                                                    >  1:10985121/1‑119 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAgctgct                                                                                                          <  2:7871033/140‑1 (MQ=255)
                          aTCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGGACGGGAATCGAgctgct                                                                                                          >  1:9117165/1‑140 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGCGCTGCTGa                                                                                                        >  2:8527777/1‑140 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAGTCGCGCTGCTGa                                                                                                        >  2:2353235/1‑140 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGATCTGCTGa                                                                                                        <  2:3132882/140‑1 (MQ=255)
                            cTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGa                                                                                                        <  1:8530851/140‑1 (MQ=255)
                             tGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGAc                                                                                                       <  2:3105278/140‑1 (MQ=255)
                                tAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGc                                                                                                    <  2:4425807/140‑1 (MQ=255)
                                 aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      <  1:10918625/107‑1 (MQ=255)
                                 aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      >  2:10918625/1‑107 (MQ=255)
                                 aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      <  1:10919143/107‑1 (MQ=255)
                                 aGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATc                                                                                                                                      >  2:10919143/1‑107 (MQ=255)
                                    tGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGGCGGGGAGCGAGCTGCTGTCCGCTGag                                                                                                >  2:5695253/1‑139 (MQ=255)
                                     gCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGata                                                                                               <  2:3322496/140‑1 (MQ=255)
                                      cGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑CCCCCCCCGGT‑CGGGAGTCGTGCTGCTGGCCGcgggtgt                                                                                              >  1:3656414/1‑134 (MQ=255)
                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCGCCGCCGGACGGTGCGCGAGCTGGTGTCCGCTGATATCCg                                                                                           >  1:7702250/1‑140 (MQ=255)
                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATGCg                                                                                           >  2:4062379/1‑140 (MQ=255)
                                         cTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTAGTGGTGCTGCTGGCCGCTGTTATGCg                                                                                           >  2:1227970/1‑140 (MQ=255)
                                          tGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGc                                                                                          <  1:6804561/140‑1 (MQ=255)
                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                       <  1:579601/140‑1 (MQ=255)
                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                       <  1:7041692/140‑1 (MQ=255)
                                             tCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAg                                                                                       <  2:10497726/140‑1 (MQ=255)
                                              cTAACGGTAGTTGCGGAAACAATTCAATGAGCGATTTCTATGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGGGCTGCTGAGCGGTGATCTccgcccgc                                                                                      >  1:3725845/1‑140 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCTATGAGCGATGTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCTTACGGTAATCGAGCTGCTGACCGCTGTTATCCGACAGCATCt                                                                                  <  2:11606945/140‑1 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGCGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  2:8148465/140‑1 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:6976495/140‑1 (MQ=255)
                                                  cGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:828311/140‑1 (MQ=255)
                                                    gTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGa                                                                                <  1:4908317/140‑1 (MQ=255)
                                                      aGTTGCGGTAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTTTTCGAGCTGCTGACCGCTGATAGACGCCAGCAGCTGACg                                                                              >  2:9857283/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              <  1:9810469/140‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACg                                                                              <  1:649369/140‑1 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGTACGGGAGGCGTGCTGCTGGCCGCTGGGATCCGCCCGCAGCTGTCg                                                                              >  2:4580844/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTTCGGTAATCGAGCTGCTGACCGGGGGTTTCCGCCTGCATGTGTCg                                                                              >  1:3460261/1‑140 (MQ=255)
                                                      aGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTACGGTTATCGTGCGGCTGGCCGCGGTTAGCCGGCAGCGGCTGACg                                                                              >  1:1144953/1‑140 (MQ=255)
                                                       gTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATAGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGc                                                                             <  2:3732922/140‑1 (MQ=255)
                                                        ttGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGcc                                                                            <  2:973701/140‑1 (MQ=255)
                                                         tGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCa                                                                           <  1:5149285/140‑1 (MQ=255)
                                                          gCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAg                                                                          <  1:8063421/140‑1 (MQ=255)
                                                             gCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTGCGGGGAGCGTGCTGCTGGCCGCGGTGAGCCGCCAGCCTCGGACGCCGGtgg                                                                       >  2:3168799/1‑138 (MQ=255)
                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:11351997/122‑1 (MQ=255)
                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  >  2:11351873/1‑122 (MQ=255)
                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCt                                                                                  <  1:11351873/122‑1 (MQ=255)
                                                                    ttCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTACGGTACTCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAc                                                                >  2:4300393/1‑140 (MQ=255)
                                                                     tCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTAcg                                                               <  2:474138/140‑1 (MQ=255)
                                                                         tGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACA‑‑GCCCCCCCCGGACGGTAAACGCGCTGGGGACCGCGGGGCTCCGCCCGCCTCTGTCGCCGGTACTCACGACGcgcc                                                           >  1:4586880/1‑140 (MQ=255)
                                                                          gAGCGATTTCTCTGTTTTCGCCGCGATCTGCGCCATCCTTGCTGCTCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCTTACGGTAATCGAGCTGCTGACCGCTGATATCCGCAAGCATCTGACGCCAGTACTCACTACGcgcca                                                          <  2:10079975/140‑2 (MQ=255)
                                                                            gCGATTTCTCTGGATTCGCCGCTATATGCGCCTGCCTTGCTGCGACCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGcgccccg                                                        <  2:5379017/140‑1 (MQ=255)
                                                                                  tctcTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCa                                                  <  1:10870038/140‑1 (MQ=255)
                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  1:9598743/140‑1 (MQ=255)
                                                                                    tctGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAAt                                                <  1:8026631/140‑1 (MQ=255)
                                                                                            cgccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGTTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTaa                                        <  2:8526383/140‑1 (MQ=255)
                                                                                             gccgcGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGATGCTGACCGCTGATATCCGCCAGCCTCTGACGCCAGTACTCACTAGGCGCCCCGCCGCCAGTGAACGTAac                                       >  2:6250422/1‑140 (MQ=255)
                                                                                             gcagcgATCTGCGCCAGCCGTGCTGCGCCCAGAGCTGGCCCCACA‑GGCCCCCCCCG‑ACGGGACTCGAGCTGCTGACCGCGGATATCCGCCCGCAGCAGACGCAAGTCCTCACGACGCGCCCCGCCCCCAATCACCGGAac                                       >  2:2115978/4‑140 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGGTGGTATCCGCCAGCGGCTGTCGGCAGTACTCTCTACGCGGCCCGCCCCCGAGCTACGTAGCACttt                                  >  1:6017040/1‑140 (MQ=255)
                                                                                                  gATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCCTCTGACGCCCGTCCTCACTACGCGCCCCGCCCGCAATCAGCGTAACACtgg                                  >  1:2287725/1‑138 (MQ=255)
                                                                                                   aTCTGCGCGAGCCTGGCTGCGCCCAGTGCGGGCCCCCCAT‑‑ACCCCCCAGTACGGTCATCGAGGTGCTGACCGCTGATATCCGCCAGCTTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCTACGTCACACTTTg                                 <  2:5801457/140‑1 (MQ=255)
                                                                                                   aTCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGGCGGTAGTCGAGCTGCTGACCGCTGTTAGCCGCCAGCATCTGAGGCCAGTCCTCACTTGGCGCCCCGCCCCCAATCTACGGTACAGTTTg                                 >  1:10719789/1‑140 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGtt                            <  2:8071236/140‑1 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGTTATCCGCCAGCATCGGACGCCAGTACTCACTACGCGCCCCGCCCCCCATCAACGTAACACGTTGCgggg                            >  1:10361048/1‑138 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCGGATCTCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCGATCAACGGAACACTTTGCgggg                            >  1:10360827/1‑138 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTACTCGAGCTGCTGACCGCGGATATCCGCCAGCAGCGGCCGCCAGGACTCACTACGCGCCCCGCCCCCAATCAACGGAACACGTTGCGGtt                            >  2:8571126/1‑140 (MQ=255)
                                                                                                        cgcCAGCCTTGCTGCGCCCAGTGCTGGCCCCAC‑‑CTCCCCCCCCGTCCGGTCATCGAGCTGCTGACCGCTGATAGCCGCCAGCATCTGACGCCAGTGCTCTCTACGCGCCGCGCCCCCCATCAAGGGAACACGTTGCGGtt                            >  2:8571514/1‑140 (MQ=255)
                                                                                                              ccTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTTCGGTCATCGAGCTGCTGTCCGCTGATATACGCCAGCATCTGACTCCAGTTCTCACTACGCGCCCCGCCCCCAATCTACGTATCACTTTGCGGGTTAATAc                      >  2:7789222/1‑140 (MQ=255)
                                                                                                               cTTGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCG‑CCCCAATCAACGTAACACTTTGCGGTTTAATACCg                    <  1:54036/140‑1 (MQ=255)
                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                    <  1:7221042/140‑1 (MQ=255)
                                                                                                                ttGCTGCGCCCAGTGCTGGCCCCAC‑‑CGCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCg                    <  2:2358025/140‑1 (MQ=255)
                                                                                                                 tgctgcGCCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTACCACTTTGCGGTTTAATACCGc                   <  2:11465547/140‑1 (MQ=255)
                                                                                                                      cgcCCAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCa              <  1:4689388/140‑1 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTATTCGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCCTg            >  1:8986698/1‑140 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCGGACGCCCGTACTCACTACGCGCCCCGCCCCCCATCCACGTGACACTGTGCGGGTGAATACCGCAGGCATg            >  1:511779/1‑140 (MQ=255)
                                                                                                                        cccAGTGCTGGCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCGGTACTCACTACGCGCCCCGCCCCCAGTCAACGTAACACTTTGCGGTTTAATACCGCAGGCATg            >  1:9355390/1‑140 (MQ=255)
                                                                                                                           aGTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTTCTCGAGCGGCGGACCGCTGAGATCCGCCCGCATCGGACGCCAGTAGTCTCTACGCGCCCCGCCCCCCAGAAAGGTAACACTTTGCGGTGTAGTACCGCAGGCGTggag         >  1:9234146/1‑137 (MQ=255)
                                                                                                                            gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg        <  1:6480320/140‑1 (MQ=255)
                                                                                                                            gTGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACg        <  1:2585866/140‑1 (MQ=255)
                                                                                                                             tGCTGGCCCCCCAG‑‑CCCCCCCCGGCCGGTAATCGAGCTGCTGACCGCGGATGTCCGCCAGCATCTGACGCCAGGACGCCCGCCGCGCCCCGCCCCCAAGCAACGTAACACTTTGCGGGTTAATCCCGCCGGCCTGCACGa       >  2:1957053/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑CCCCCCCCGGT‑CGGTAATCGTGCTGCTGTGCGCTGATATCCGCCAGCAGCTGACGCCAGTTCTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGGTGTATACGGCAGGCCTGCTCgg       >  1:7206992/1‑139 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTCCGGTAATCGAGCTGCTGTCCGCTGATTTCCGCCAGCATCTGACGCCAGTACGCACGACGGGCCCCGCCCCCCTTCAACGTCACACTTTGCGGTTTAATACCGCAGGCATGCGCGa       >  1:2842006/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCCGTACTCACTACGCGCCCCGCCCCCAATCCACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACgc       >  2:1956895/1‑139 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa       >  2:8340411/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCGGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGa       >  2:6845649/1‑140 (MQ=255)
                                                                                                                             tGCTGGCCCCACAT‑‑CCCCCCCCGTACGGGAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCCCTCAACGTAACACGTTGCGGTTTAATACCGCAGGCATGCACgc       >  2:10435199/1‑139 (MQ=255)
                                                                                                                                tGGCCCCACAT‑‑CCCCCCCCGTACGGTAAACGAGCTGCTGACCGCTGATATCCGCCCGCATCTGACGCCAGTACTCACAACGCGCCCCGCCCCCAATCAACGTAACACATTGCGGGTTAATACCGCAGGCATGCACGACAt    >  1:9072240/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGCCGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACGTTGCGGTTTAATACCGCAGGCCTGCACGACATcc  >  2:6773566/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACGCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc  >  2:742128/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGGACGGTCATCGAGCTGCTGGCCGCGGATATCCGCCAGCATCTGACGCCAGTACGCACAACGCGCCCCGCCCCCAATCCACGGAACACGTTGCGGGTTGATACCGCAGGCATGCACGACATcc  >  1:1805309/1‑140 (MQ=255)
                                                                                                                                  gCCCCACAT‑‑CCCCCCCCGGACGGTAATCGAGCTGCTGCCCGCGGATATCCGCCCGCCTCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGGAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATcc  >  2:6774470/1‑140 (MQ=255)
                                                                                                                                           ||                                                                                                                                   
GGCTGATAAGCGGCATAACGCTGCGCATCTGGTAGATGCGACTGTTCTAACGGTAGTTGCGGCAACAATTCAATGAGCGATTTCTCTGGATTCGCCGCGATCTGCGCCAGCCTTGCTGCGCCCAGTGCTGGCCCCACATCCCCCCCCCCGTACGGTAATCGAGCTGCTGACCGCTGATATCCGCCAGCATCTGACGCCAGTACTCACTACGCGCCCCGCCCCCAATCAACGTAACACTTTGCGGTTTAATACCGCAGGCATGCACGACATCC  >  NZ_CP025268/3728661‑3728932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑