Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 3,982,878 (G)8→6 pseudogene (72‑73/1388 nt) yifK → amino acid permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,982,8720G.99.5% 2933.8 / 2.3 615pseudogene (66/1388 nt)yifKamino acid permease
Reads supporting (aligned to +/- strand):  ref base G (3/0);  new base . (371/241);  total (374/241)
Fisher's exact test for biased strand distribution p-value = 2.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NZ_CP0252683,982,8730G.98.2% 2848.1 / 33.6 615pseudogene (67/1388 nt)yifKamino acid permease
Reads supporting (aligned to +/- strand):  ref base G (11/0);  new base . (362/240);  total (374/241)
Fisher's exact test for biased strand distribution p-value = 4.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 641 total aligned reads displayed.

CATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT  >  NZ_CP025268/3982733‑3983005
                                                                                                                                           ||                                                                                                                                    
    tGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggg                                                                                                                                   >  1:7749745/1‑140 (MQ=255)
     gCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTgggggg                                                                                                                                  <  2:5335983/140‑1 (MQ=255)
       aaCACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTggggggca                                                                                                                                <  2:1679689/140‑3 (MQ=255)
           cTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGGAGCTCGACATATCGAACTCATCGCCCTggggggcaccat                                                                                                                            >  1:10436438/1‑134 (MQ=255)
             cGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTg                                                                                                                        >  2:10867263/1‑140 (MQ=255)
                tGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCg                                                                                                                     <  1:8559879/140‑1 (MQ=255)
                  gaCCCATAACGATAACCGTTAACACCGGAAAGCATGCAAACACAACACGACGATTTATGGCAGATAACAACCCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTc                                                                                                                   <  2:10193022/139‑1 (MQ=255)
                   aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  2:4544027/1‑140 (MQ=255)
                   aCCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGAGTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCg                                                                                                                  >  1:1823334/1‑140 (MQ=255)
                     ccATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGc                                                                                                                <  1:8118028/140‑1 (MQ=255)
                      cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGcc                                                                                                               >  2:9302104/1‑140 (MQ=255)
                      cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGcc                                                                                                               <  1:2318302/140‑1 (MQ=255)
                      cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGcc                                                                                                               >  2:2129935/1‑140 (MQ=255)
                      cATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCAGTGGCGTCGGcc                                                                                                               >  1:7571190/1‑140 (MQ=255)
                        tAACTATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCGCT‑‑GGGGGGCACCATAGGCGTCGGccgg                                                                                                             >  2:7635280/1‑138 (MQ=255)
                        tAACGATAACCGGTAACACCGGAACGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGccgg                                                                                                             >  1:2639969/1‑138 (MQ=255)
                        tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTTTGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTg                                                                                                             <  2:2468274/140‑1 (MQ=255)
                        tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTg                                                                                                             >  1:2177666/1‑140 (MQ=255)
                        tAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTg                                                                                                             >  1:11325393/1‑140 (MQ=255)
                         aaCGATAACCGGTAACACCGGACAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCC‑‑GGGGGGGCACCATTGGCGTCGGCCTGt                                                                                                            >  2:547014/1‑140 (MQ=255)
                          aCGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGGCTGtt                                                                                                           >  2:3403068/1‑140 (MQ=255)
                            gATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTa                                                                                                         <  2:9703418/140‑1 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                       >  1:10930300/1‑140 (MQ=255)
                              tAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                       >  1:5033954/1‑140 (MQ=255)
                                 ccGGTAACACCGGAAAGCATGCCAACACAACACGCGGATTAATGGCAGAAAACAAACCAGAGCTCCAGCGTGGGCTGGAAGCTCGACAAATCGAACTCATCGCCCT‑‑GGGGGGCCCCATTGGCGTCGGCCTGTTTAAgggg                                                                                                    <  1:998686/140‑1 (MQ=255)
                                 ccGGTAACACCGGAAAGCATGCACACACACCACGAGCATTTATGGCAGATAACAAACCAGAGCCACAGCGAGGGCTGGAAGCTCCACATAGCGAACTCAACCCCCT‑‑GGGGGGCACCAGTGGCGTCGCCCTGTTTATgggg                                                                                                    <  2:7195891/140‑1 (MQ=255)
                                 ccGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATgggg                                                                                                    >  1:8306984/1‑140 (MQ=255)
                                     tAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccg                                                                                                >  1:9436073/1‑140 (MQ=255)
                                      aaCACCGGAAAGCATGCAAACACAACCCGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc                                                                                               <  1:4381498/140‑1 (MQ=255)
                                      aaCACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGgccgc                                                                                               >  1:5278318/1‑140 (MQ=255)
                                        cacCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATCTCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCa                                                                                             <  2:9532002/140‑1 (MQ=255)
                                         acCGGAAAGCATGCAAACACACCACGAGGAGTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAg                                                                                            <  2:7561614/140‑1 (MQ=255)
                                            ggAAAGCATGCAAACACAACACGAGGATTTATGGAAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAc                                                                                         >  1:35284/1‑140 (MQ=255)
                                             gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCCCCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        >  2:3803751/1‑140 (MQ=255)
                                             gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        <  1:5600559/140‑1 (MQ=255)
                                             gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        <  2:70070/140‑1 (MQ=255)
                                             gAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTAcc                                                                                        <  2:4093403/140‑1 (MQ=255)
                                                  cATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                                   <  2:2998268/140‑1 (MQ=255)
                                                  cATGCAAACACAACACGAGGATTTATGGCAGAGAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaa                                                                                   <  2:741599/140‑1 (MQ=255)
                                                   aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATg                                                                                                       >  2:8252671/1‑119 (MQ=255)
                                                   aTGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGaaa                                                                                  >  1:11498886/1‑140 (MQ=255)
                                                     gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAACTg                                                                                >  2:7863573/1‑140 (MQ=255)
                                                     gCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATg                                                                                >  1:1553216/1‑140 (MQ=255)
                                                       aaaCACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATggg                                                                              <  1:911834/140‑1 (MQ=255)
                                                        aacacaacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggc                                                                             <  2:8571260/140‑1 (MQ=255)
                                                             aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggc                                                                        <  2:2820342/140‑1 (MQ=255)
                                                             aacacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTGTAGGGGGGCCGCCCGGCCCCGGAAATgggccgggc                                                                        >  2:8005099/1‑140 (MQ=255)
                                                              acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                       <  1:6474816/140‑1 (MQ=255)
                                                              acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                       <  1:9516798/140‑1 (MQ=255)
                                                              acacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATgggccgggcc                                                                       <  2:5627208/140‑1 (MQ=255)
                                                               cacGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCa                                                                      >  1:1733498/1‑140 (MQ=255)
                                                                 cGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATc                                                                    >  1:7950116/1‑140 (MQ=255)
                                                                 cGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATc                                                                    >  1:5871302/1‑140 (MQ=255)
                                                                   aGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                  <  2:5905488/140‑1 (MQ=255)
                                                                    ggATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGt                                                                 <  1:3724673/140‑1 (MQ=255)
                                                                     gATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTa                                                                >  1:6095597/1‑140 (MQ=255)
                                                                       tttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAtt                                                              >  1:6687131/1‑140 (MQ=255)
                                                                        ttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                             <  1:10243230/140‑1 (MQ=255)
                                                                        ttATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttg                                                             <  1:2781146/140‑1 (MQ=255)
                                                                         tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                            >  2:3118065/1‑140 (MQ=255)
                                                                         tATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATAGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAttgt                                                            <  2:3968978/140‑1 (MQ=255)
                                                                           tGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATACGTAttgttg                                                          <  2:5026488/140‑1 (MQ=255)
                                                                            ggCAGATAACAAACCAGAGCTCCAGCGTGGGCTGGAAGCTCGACATATCGATCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTTTTGTTgg                                                         <  2:11384537/140‑1 (MQ=255)
                                                                             gCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGc                                                        <  2:1744793/140‑1 (MQ=255)
                                                                             gCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGc                                                        <  2:1062983/140‑1 (MQ=255)
                                                                              cAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGcc                                                       <  1:3316042/140‑1 (MQ=255)
                                                                                gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCta                                                     >  2:10468556/1‑140 (MQ=255)
                                                                                gATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCta                                                     >  2:475966/1‑140 (MQ=255)
                                                                                 aTAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCtat                                                    >  1:9277677/1‑140 (MQ=255)
                                                                                           agagCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCg                                          >  1:5420049/1‑140 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                        <  2:11513183/140‑1 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCGGTTTATTGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCggg                                        >  1:7352729/1‑140 (MQ=255)
                                                                                             agCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCGGTGTTGGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCGATATCATCGCCggg                                        >  2:6879259/1‑140 (MQ=255)
                                                                                                  cAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGtt                                   >  1:4770569/1‑140 (MQ=255)
                                                                                                    gCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCg                                 <  2:6596009/140‑1 (MQ=255)
                                                                                                       tGGGCTGGAAGCTGGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                              >  1:11046039/1‑140 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCg                                                                  <  2:1892805/104‑1 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTtcttct                              >  2:1374111/1‑140 (MQ=255)
                                                                                                       tGGGCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCt                              >  2:2745194/1‑140 (MQ=255)
                                                                                                         ggCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTCTTGTTGGCCTATATCATCGCCGGGCTGTTCGTCttt                            >  1:4470780/1‑140 (MQ=255)
                                                                                                         ggCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTAGATCATCGCCGGGCTGTTCGTCttt                            >  2:3681526/1‑140 (MQ=255)
                                                                                                          gCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCtttt                           >  1:3923622/1‑140 (MQ=255)
                                                                                                          gCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTAGTGTTGGCCTATATCATCGCCGGGCTGTTCGTCtttt                           >  2:8932954/1‑140 (MQ=255)
                                                                                                          gCTGGAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTGTATGGGGGCCGCCAGTAACCTGAAATGGGCCGGGCCCTCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTGtttt                           >  2:2285881/1‑140 (MQ=255)
                                                                                                              gAAGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcat                       >  1:2982375/1‑140 (MQ=255)
                                                                                                               aaGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTTGTCTTTTtcatc                      <  2:2556194/140‑1 (MQ=255)
                                                                                                                aGCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTtcatca                     <  1:10633253/140‑1 (MQ=255)
                                                                                                                 gCTCGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTcatcat                    >  1:1362234/1‑140 (MQ=255)
                                                                                                                   tCGACATCTCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGc                  <  1:8709246/140‑1 (MQ=255)
                                                                                                                    cGACATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCg                 <  1:125345/140‑1 (MQ=255)
                                                                                                                       cATATCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTc              <  1:6879518/140‑1 (MQ=255)
                                                                                                                         tatCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTGATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCaa            >  1:4036828/1‑140 (MQ=255)
                                                                                                                          atCGAACTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCGGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAAt           >  2:9115377/1‑140 (MQ=255)
                                                                                                                               aCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATGCGTATTGTTGGCCTATATCTTCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      >  2:6370394/1‑140 (MQ=255)
                                                                                                                               aCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      <  1:8932724/140‑1 (MQ=255)
                                                                                                                               aCTCATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      <  2:1223937/140‑1 (MQ=255)
                                                                                                                                 tCATCGCCCT‑‑GGGGGGCACCCTTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGaa    <  1:7127475/140‑1 (MQ=255)
                                                                                                                                  cATCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCg      >  1:10058186/1‑137 (MQ=255)
                                                                                                                                   aTCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCGGGGTGAGGGGGGCCGCCAGTACCCTGAAATGGGCCGGTCCATCCGTAATGTTGGCCTATATCATCGCCGGGCGGTTCGTCGTTTTCATCATGCGTGCAAAGGGCGAAAt  >  2:6070931/1‑140 (MQ=255)
                                                                                                                                   aTCGCCCT‑‑GGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAt  >  2:4255854/1‑140 (MQ=255)
                                                                                                                                           ||                                                                                                                                    
CATTTGCAACACTCGATGTACCCATAACGATAACCGGTAACACCGGAAAGCATGCAAACACAACACGAGGATTTATGGCAGATAACAAACCAGAGCTACAGCGTGGGCTGGAAGCTCGACATATCGAACTCATCGCCCTGGGGGGGGCACCATTGGCGTCGGCCTGTTTATGGGGGCCGCCAGTACCCTGAAATGGGCCGGGCCATCCGTATTGTTGGCCTATATCATCGCCGGGCTGTTCGTCTTTTTCATCATGCGTTCAATGGGCGAAAT  >  NZ_CP025268/3982733‑3983005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑