BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,224,095	+GA	coding (248/249 nt)	CXP41_RS06440 →	protein YmgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,224,094	1	.	A	99.4%	8059.6 / 23.2	2094	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (5/8); new base A (814/1266); total (820/1274)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	NZ_CP025268	1,224,094	2	.	G	99.4%	7327.9 / 24.0	2094	coding (247/249 nt)	CXP41_RS06440	protein YmgC
Reads supporting (aligned to +/- strand): ref base . (5/8); new base G (815/1266); total (820/1274)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 2253 total aligned reads displayed.

TCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑A‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGAC  >  NZ_CP025268/1223955‑1224233

||

  aGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAATGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑ag                                                                                                                                             >  2:10281505/1‑139 (MQ=255)

    gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaa                                                                                                                                           >  1:8542630/1‑137 (MQ=255)

    gCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑agaa                                                                                                                                           >  1:11537238/1‑137 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTa                                                                                                                                   <  2:5301137/140‑1 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTa                                                                                                                                   <  1:3652745/140‑1 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTa                                                                                                                                   <  1:2605861/140‑1 (MQ=255)

            cTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTa                                                                                                                                   <  2:1603992/140‑1 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAg                                                                                                                                 >  2:2675927/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAg                                                                                                                                 >  1:7558790/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAg                                                                                                                                 >  2:6052105/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAg                                                                                                                                 >  2:10664271/1‑140 (MQ=255)

              tGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAg                                                                                                                                 >  2:2160491/1‑140 (MQ=255)

               gCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAgg                                                                                                                                >  2:4436260/1‑140 (MQ=255)

                  tttAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTACTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGttt                                                                                                                             <  2:5692277/140‑1 (MQ=255)

                       aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGaa                                                                                                                        <  2:3810343/140‑1 (MQ=255)

                       aaGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGaa                                                                                                                        <  2:5127550/140‑1 (MQ=255)

                          aTGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATaa                                                                                                                     <  1:7738602/140‑1 (MQ=255)

                            gAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAAc                                                                                                                   <  1:5746086/140‑1 (MQ=255)

                              aaCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACAt                                                                                                                 <  2:7933309/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCATATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  1:14831/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  2:3571954/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  2:4238057/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  2:1451256/140‑1 (MQ=255)

                               aCCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                <  1:9494928/140‑1 (MQ=255)

                                 cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGACTCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                              <  2:9018144/140‑1 (MQ=255)

                                 cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                              <  2:885438/140‑1 (MQ=255)

                                 cAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAg                                                                                                              <  2:10488294/140‑1 (MQ=255)

                                   gtgtGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCt                                                                                                            >  1:5145017/1‑140 (MQ=255)

                                    tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                           <  2:3310439/140‑1 (MQ=255)

                                    tgtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTg                                                                                                           <  2:7458897/140‑1 (MQ=255)

                                     gtgATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGt                                                                                                          <  2:11321536/140‑1 (MQ=255)

                                        aTTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATc                                                                                                       >  1:4959542/1‑140 (MQ=255)

                                             ttctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCAt                                                                                                  >  1:3883295/1‑140 (MQ=255)

                                              tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                                 <  2:1099563/140‑1 (MQ=255)

                                              tctGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATg                                                                                                 <  2:3139653/140‑1 (MQ=255)

                                                   tctcTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGAt                                                                                            <  1:5517364/140‑1 (MQ=255)

                                                       tATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCaa                                                                                        >  1:129019/1‑140 (MQ=255)

                                                         tCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAg                                                                                      >  1:4307039/1‑140 (MQ=255)

                                                           tGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTg                                                                                    >  2:2458323/1‑140 (MQ=255)

                                                           tGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTg                                                                                    <  1:7936954/140‑1 (MQ=255)

                                                               aaTATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCa                                                                                >  1:10985931/1‑140 (MQ=255)

                                                                   ttGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATc                                                                                                                >  1:11157927/1‑104 (MQ=255)

                                                                     gCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAg                                                                          <  2:2971221/140‑1 (MQ=255)

                                                                     gCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAg                                                                          >  1:10430636/1‑140 (MQ=255)

                                                                        gTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCg                                                                       <  1:10269750/140‑1 (MQ=255)

                                                                         tACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGt                                                                      >  1:3371864/1‑140 (MQ=255)

                                                                           cATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTa                                                                    >  2:6238319/1‑140 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  1:9304357/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  2:941473/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  2:6408645/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  1:22528/140‑1 (MQ=255)

                                                                             ttGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATg                                                                  <  2:11480241/140‑1 (MQ=255)

                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                                <  2:1960015/140‑1 (MQ=255)

                                                                               gAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCt                                                                <  2:9229978/140‑1 (MQ=255)

                                                                                aaaTGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCtt                                                               >  1:4757829/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGttt                                                                                                                             <  1:7246361/73‑1 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                          >  1:4009118/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                          >  1:10820908/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCtta                                                          >  1:6153234/1‑140 (MQ=255)

                                                                                     cATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATGTCAGGCGTGATGCTGTCtta                                                          >  1:6589364/1‑140 (MQ=255)

                                                                                       tAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttatt                                                        <  2:7319502/140‑1 (MQ=255)

                                                                                       tAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCttatt                                                        <  1:11337042/140‑1 (MQ=255)

                                                                                           aCTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGt                                                    <  1:1001044/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                                  <  1:9710926/140‑1 (MQ=255)

                                                                                             tACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTcc                                                  <  2:4891653/140‑1 (MQ=255)

                                                                                              acacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                                 <  1:4511171/140‑1 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTa                                                                                                         <  2:10082286/83‑1 (MQ=255)

                                                                                               cacTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                                >  1:2928437/1‑140 (MQ=255)

                                                                                               cacTAACGGAAATCATTATTCATGACAGGGACTCCGTGCTCTCTAAG‑‑A‑‑ACATGAGTACGGTTATAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGc                                                <  2:8604331/140‑1 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                           >  2:9795867/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                           >  1:2045/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                           >  1:2357400/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                           >  1:6391247/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCAAGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                           >  2:7124984/1‑140 (MQ=255)

                                                                                                    aCGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑AGTAGTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACAATGCTGATGCAAAGTGAGCATTTAAGGCGTTATGCTTTCTTATTATGTCAGCAatat                                           >  2:6693723/1‑140 (MQ=255)

                                                                                                       gATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAg                                        <  1:5372118/140‑1 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                                      >  1:10621267/1‑140 (MQ=255)

                                                                                                         tatCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACGTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgt                                      >  2:548307/1‑140 (MQ=255)

                                                                                                          atCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAatat                                           >  2:8369756/1‑134 (MQ=255)

                                                                                                          atCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtg                                     >  2:1213327/1‑140 (MQ=255)

                                                                                                          atCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtg                                     <  2:7739221/140‑1 (MQ=255)

                                                                                                           tCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCg                                                 <  2:6923121/127‑1 (MQ=255)

                                                                                                           tCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGgtgt                                    <  1:1870001/140‑1 (MQ=255)

                                                                                                                attaATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGa                               <  2:8153302/140‑1 (MQ=255)

                                                                                                                attaATGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGa                               >  1:6809455/1‑140 (MQ=255)

                                                                                                                     tGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGga                          <  2:9626897/140‑1 (MQ=255)

                                                                                                                     tGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGga                          <  2:5951715/140‑1 (MQ=255)

                                                                                                                     tGACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCAGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGga                          >  1:7037305/1‑140 (MQ=255)

                                                                                                                      gACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag                         <  2:4693264/140‑1 (MQ=255)

                                                                                                                      gACAGGGAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGgag                         >  1:815764/1‑140 (MQ=255)

                                                                                                                           ggAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc                    >  2:3981314/1‑140 (MQ=255)

                                                                                                                           ggAATCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtc                    >  1:9883239/1‑140 (MQ=255)

                                                                                                                             aaTCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTtctc                  <  2:2317533/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAATTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATTTCAGGTGTCAAGAATGGAGAGTTCTCg                 <  1:8806863/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg                 <  1:8997960/140‑1 (MQ=255)

                                                                                                                              aTCCGTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCg                 <  1:5984230/140‑1 (MQ=255)

                                                                                                                                  gTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGctct             <  1:1687941/140‑1 (MQ=255)

                                                                                                                                  gTGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGctct             <  2:1796180/140‑1 (MQ=255)

                                                                                                                                   tGCTCTCT‑‑TAGA‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCtctc            >  1:3133958/1‑140 (MQ=255)

                                                                                                                                            at‑‑A‑‑AGTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAAGGGAGTGTTCTCGCTCTCCATTCTTGa   <  1:8342824/139‑1 (MQ=255)

||

TCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCAGTACATTGAAATGCATAAGACTACACTAACGGATATCATTATTAATGACAGGGAATCCGTGCTCTCT‑‑T‑‑A‑‑ACTTGAGTAAGGTTTTAGAATAAACATCAGCTGTATCACCATGCTGATGCAAAGTGAGCATTTCAGGCGTTATGCTTTCTTATTATGTCCGCAATATCAGGTGTCAAGAATGGAGAGTTCTCGCTCTCCATTCTTGAC  >  NZ_CP025268/1223955‑1224233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`