Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 1,826,683 (A)7→8 intergenic (‑247/+51) chbB ← / ← osmE PTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252681,826,6761.A94.7% 2766.5 / 119.1 760intergenic (‑240/+58)chbB/osmEPTS N,N'‑diacetylchitobiose transporter subunit IIB/osmotically‑inducible lipoprotein OsmE
Reads supporting (aligned to +/- strand):  ref base . (34/6);  new base A (476/243);  total (511/249)
Fisher's exact test for biased strand distribution p-value = 1.46e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 770 total aligned reads displayed.

GCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826541‑1826810
                                                                                                                                        |                                                                                                                                           
                ggTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATACTTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATgg                                                                                                                     >  1:7776609/1‑140 (MQ=255)
                ggTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATgg                                                                                                                     <  1:9326196/140‑1 (MQ=255)
                  tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAGTTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACTTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGcc                                                                                                                   >  2:8339022/1‑140 (MQ=255)
                  tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGcc                                                                                                                   >  2:4558728/1‑140 (MQ=255)
                  tataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGcc                                                                                                                   >  2:5914507/1‑140 (MQ=255)
                   ataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGcag                                                                                                                  >  2:10806002/1‑138 (MQ=255)
                   ataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCg                                                                                                                  >  1:6313262/1‑140 (MQ=255)
                   ataGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCg                                                                                                                  >  2:787814/1‑140 (MQ=255)
                     aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTCAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGt                                                                                                                >  2:2146154/1‑140 (MQ=255)
                     aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGt                                                                                                                >  2:2409191/1‑140 (MQ=255)
                     aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGt                                                                                                                >  1:4590931/1‑140 (MQ=255)
                     aGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGt                                                                                                                >  1:3097272/1‑140 (MQ=255)
                       aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:6525912/1‑140 (MQ=255)
                       aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:8587049/1‑140 (MQ=255)
                       aCCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGttt                                                                                                              >  1:6812472/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTCAGAACTCAGGCC‑‑A‑AA‑AA‑AATCCGGCGCAATGGCCGGTTGCCgtt                                                                                                         >  2:11255936/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAATAACTCAGGCCA‑A‑AA‑AA‑AA‑CAGGCGCAATGTCCGGTTTCCgtt                                                                                                         >  2:11059566/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCAAA‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgt                                                                                                          >  1:4271498/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:2549587/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:5130302/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:7943712/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:8652834/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:5953592/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:3203392/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:10789277/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:11577382/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:3901568/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:10015245/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:2759124/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:2289122/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTATCCgtt                                                                                                         >  2:482264/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑‑A‑AATAA‑AA‑CCGGCGCAATGGCCGGTTTCCgta                                                                                                         >  2:6376002/1‑139 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑‑A‑AA‑AA‑AATCCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  2:10936069/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTAGGGCAAATTATTCGCGAAGATAATTAAGAACTCAGGCC‑‑A‑AA‑AATAA‑CCGGCGCAATGGCCGGTTGCCgtt                                                                                                         >  2:4679977/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACAGTTCGGGCCAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTGTCCgtt                                                                                                         >  1:5450137/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGGGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCgtt                                                                                                         >  1:3374207/1‑140 (MQ=255)
                            aGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGGGGTTATGAACCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAAAAAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCCCAATGGCCGGTTTCCgtt                                                                                                         >  1:10235248/1‑140 (MQ=255)
                                 tAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTAc                                                                                                    >  2:1520193/1‑140 (MQ=255)
                                    tATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCa                                                                                                 <  1:4033685/140‑1 (MQ=255)
                                      tttCTTTGAGCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGACGTCAGGCC‑‑ACAT‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCTAg                                                                                               >  2:8904762/1‑140 (MQ=255)
                                      tttCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAg                                                                                               >  2:5222065/1‑140 (MQ=255)
                                           ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTc                                                                                          >  2:6759306/1‑140 (MQ=255)
                                           ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTc                                                                                          >  1:5043350/1‑140 (MQ=255)
                                           ttGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTc                                                                                          >  2:2622288/1‑140 (MQ=255)
                                            tGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCa                                                                                         >  2:1637172/1‑140 (MQ=255)
                                              aCCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTGGTTACTCAAGCTTTCAGa                                                                                       >  2:10619232/1‑140 (MQ=255)
                                                  tataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                                   <  1:11623806/140‑1 (MQ=255)
                                                  tataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                                   >  1:5761519/1‑140 (MQ=255)
                                                  tataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATACGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGaa                                                                                   >  1:9798338/1‑140 (MQ=255)
                                                   ataAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAt                                                                                  >  2:9482077/1‑140 (MQ=255)
                                                    taAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAAtt                                                                                 >  1:7270009/1‑140 (MQ=255)
                                                        ttGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGAtt                                                                             <  1:8100759/140‑1 (MQ=255)
                                                          gTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGGCGAATTGATTAc                                                                           >  1:8928767/1‑140 (MQ=255)
                                                             tctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTc                                                                        >  2:569419/1‑140 (MQ=255)
                                                              ctcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCg                                                                       <  1:2020856/140‑1 (MQ=255)
                                                               tcCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGc                                                                      <  2:10337889/140‑1 (MQ=255)
                                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:6946385/140‑1 (MQ=255)
                                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:7448899/140‑1 (MQ=255)
                                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:4383930/140‑1 (MQ=255)
                                                                ccTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCa                                                                     <  1:1569044/140‑1 (MQ=255)
                                                                   tGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGcc                                                                  >  1:7081417/1‑140 (MQ=255)
                                                                     aCCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCtg                                                                <  1:527797/140‑1 (MQ=255)
                                                                         gtgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGa                                                            >  1:1131343/1‑140 (MQ=255)
                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           <  1:8334808/140‑1 (MQ=255)
                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           <  1:3736477/140‑1 (MQ=255)
                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           >  2:9034183/1‑140 (MQ=255)
                                                                          tgGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAAGGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGAt                                                           <  2:1922870/140‑1 (MQ=255)
                                                                            gTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAAATAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCa                                                         <  2:9695451/140‑1 (MQ=255)
                                                                               aTGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAgtg                                                      <  1:5606346/140‑1 (MQ=255)
                                                                               aTGACCCTCTTCACATTTCGGGCAAATAAGTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTGTCAGACGAATTGTGTACTTCGCAGCCGGTGGCTCAgtg                                                      >  2:6254553/1‑140 (MQ=255)
                                                                                 gACCCTCTTCACATTTCTGGCAAATTATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTc                                                    <  1:1420698/140‑1 (MQ=255)
                                                                                    ccTCTTCACATTTCGGGCAAATACTTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  1:10996920/1‑140 (MQ=255)
                                                                                    ccTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  2:432117/1‑140 (MQ=255)
                                                                                    ccTCTTCACATTTCGGGCAAATAATGCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGTCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTa                                                 >  2:3111728/1‑140 (MQ=255)
                                                                                       ctTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTc                                              >  1:3003949/1‑140 (MQ=255)
                                                                                        ttCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCa                                             >  2:3780371/1‑140 (MQ=255)
                                                                                         tCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAg                                            <  2:7007228/140‑1 (MQ=255)
                                                                                         tCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAg                                            >  1:6659713/1‑140 (MQ=255)
                                                                                           acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  2:4155883/140‑1 (MQ=255)
                                                                                           acaTTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGca                                          <  1:9101720/140‑1 (MQ=255)
                                                                                              tttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAg                                       >  2:3459195/1‑140 (MQ=255)
                                                                                               ttCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAgg                                      >  1:1873939/1‑140 (MQ=255)
                                                                                                tCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGt                                     >  2:5479986/1‑140 (MQ=255)
                                                                                                 cGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTc                                    >  1:9592288/1‑140 (MQ=255)
                                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGGGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                   >  1:10840720/1‑140 (MQ=255)
                                                                                                  gggCAAATAATTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCt                                   >  1:4744753/1‑140 (MQ=255)
                                                                                                       aataatTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATaa                              >  1:6887862/1‑140 (MQ=255)
                                                                                                        agaatTCGCGAAGATCATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCACTGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAAc                             <  2:7511060/138‑1 (MQ=255)
                                                                                                          aatTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAAGGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCg                           <  1:7624840/140‑1 (MQ=255)
                                                                                                           atTCGCGAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCgg                          <  1:10606768/140‑1 (MQ=255)
                                                                                                             tCGCTAAGATATTTAAGAACTCTGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCAGGTTTCCGTTGTTACTCTATCTTTCAGTCGAATTGATTACTTCGCAGCATGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAg                        <  2:6552658/140‑1 (MQ=255)
                                                                                                              cgcgAAGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGt                       >  1:3588972/1‑140 (MQ=255)
                                                                                                                  caGATAAGTAAGCCCACAGGCC‑‑ACAC‑AA‑AA‑CCGCCGCAATCGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATGGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATCACCGGAGTtgat                   <  2:8134226/139‑1 (MQ=255)
                                                                                                                   aGATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgatg                  >  2:10502168/1‑140 (MQ=255)
                                                                                                                    gATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgatga                 >  1:7730339/1‑140 (MQ=255)
                                                                                                                    gATAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTtgatga                 >  1:11123121/1‑140 (MQ=255)
                                                                                                                     aTAATTAAGAACTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGAc                <  1:972570/140‑1 (MQ=255)
                                                                                                                               aCTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  2:7633578/1‑140 (MQ=255)
                                                                                                                               aCTCAGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTa      >  2:2867638/1‑140 (MQ=255)
                                                                                                                                   aGGCCA‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTatcat  >  2:3946287/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                           
GCTGAAAGGAGTATACGGTATAGACCTGAGCAATAGTATTTCTTTGACCTTATAAATTGTTTCTCCTTGACCTGTGGTTATGACCCTCTTCACATTTCGGGCAAATAATTCGCGAAGATAATTAAGAACTCAGGCC‑‑A‑AA‑AA‑AA‑CCGGCGCAATGGCCGGTTTCCGTTGTTACTCAAGCTTTCAGACGAATTGATTACTTCGCAGCCTGTGGATCAGTGTCGTATTCAGCACAGGTCTGATAACCGGAGTTGATGACATGTCCGGTATCAT  >  NZ_CP025268/1826541‑1826810

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑