BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	1,873,836	(T)_5→6	pseudogene (67/1283 nt)	yeaH →	YeaH/YhbH family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	1,873,831	1	.	T	97.3%	2175.0 / 40.1	596	pseudogene (62/1283 nt)	yeaH	YeaH/YhbH family protein
Reads supporting (aligned to +/- strand): ref base . (11/5); new base T (352/225); total (365/231)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 675 total aligned reads displayed.

AACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCA  >  NZ_CP025268/1873693‑1873965

    aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑ttttt                                                                                                                                   >  1:1602037/1‑140 (MQ=255)

    aCAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑ttttt                                                                                                                                   >  2:1439834/1‑140 (MQ=255)

     cAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑tttttt                                                                                                                                  >  2:8203315/1‑139 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑tttttta                                                                                                                                 <  2:8425595/140‑3 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑tttttta                                                                                                                                 >  2:5112346/1‑138 (MQ=255)

      aGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑tttttta                                                                                                                                 <  1:7457042/140‑3 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AACAACAAAAGCATGGTGAATCGCCAGCG‑ttttttacg                                                                                                                               <  2:2231270/140‑5 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑ttttttacg                                                                                                                               <  1:10220991/140‑5 (MQ=255)

        cGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑ttttttacg                                                                                                                               >  1:5122464/1‑136 (MQ=255)

         gAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑ttttttacgc                                                                                                                              >  2:2404664/1‑135 (MQ=255)

               tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTtat                                                                                                                         >  2:5031335/1‑140 (MQ=255)

               tGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGGAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGACCGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTtat                                                                                                                         >  2:10096277/1‑140 (MQ=255)

                gggCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTtata                                                                                                                        <  1:10031011/140‑1 (MQ=255)

                    cTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAgc                                                                                                                    <  1:10427315/140‑1 (MQ=255)

                       agaAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCa                                                                                                                 >  2:1857404/1‑140 (MQ=255)

                         aaGCGGCAACACAGGCGTAGCATACAGTTGTCAAATGGAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AACACCAAAAGCCTGGTGAATCGCCAGCGTTTTTTACGCCGTTATACAGCGCaca                                                                                                               <  2:967866/140‑3 (MQ=255)

                         aaGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCaaa                                                                                                               <  2:1546245/140‑1 (MQ=255)

                         aaGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCaaa                                                                                                               <  1:3986596/140‑1 (MQ=255)

                            cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTa                                                                                                            >  2:2959530/1‑140 (MQ=255)

                            cGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTa                                                                                                            >  1:4685490/1‑140 (MQ=255)

                              gCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTaaa                                                                                                          <  2:3216566/140‑1 (MQ=255)

                                    caGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCg                                                                                                    <  2:8678413/140‑1 (MQ=255)

                                           aGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAACGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCg                                                                                             <  1:8325326/140‑1 (MQ=255)

                                            gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                            >  2:7560511/1‑140 (MQ=255)

                                            gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                            <  1:4738738/140‑1 (MQ=255)

                                            gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                            >  1:8108178/1‑140 (MQ=255)

                                            gCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGa                                                                                            <  1:7664473/140‑1 (MQ=255)

                                            ccagaCAGTTGGCAAAGGGAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAACGCGCAAATTAAACAGTCGATCTCCGa                                                                                            <  2:7153254/136‑1 (MQ=255)

                                             cATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAg                                                                                           >  2:11467621/1‑140 (MQ=255)

                                              ctACCGTTGGCAACTGTAGGCCGGGGGGCCACTGACCTTGTGTATTGACCGGCGTCTGAACGGC‑AAACACAAAAGCATGGTGACTCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  2:10876398/139‑1 (MQ=255)

                                              aTACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  1:2710172/140‑1 (MQ=255)

                                              aTACAGTTGGCAAAGGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  2:1395045/140‑1 (MQ=255)

                                               aaCAGGTGGCAAAAGGTTTACGGGGGGCCTACGCCCTGGTTTATTGCCCCGCGTAGGCAAGGCAAAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  1:10850959/139‑1 (MQ=255)

                                                aCAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGcc                                                                                        <  1:4939353/140‑1 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          >  2:9152753/1‑137 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAgg                                                                                          <  1:9152753/137‑1 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                       >  2:5181785/1‑140 (MQ=255)

                                                 cAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCa                                                                                       >  1:5686813/1‑140 (MQ=255)

                                                   gTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAtt                                                                                     >  1:5885661/1‑140 (MQ=255)

                                                       gCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                 >  1:7052124/1‑140 (MQ=255)

                                                       gCAAATGTAGGACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaata                                                                                 >  2:4050660/1‑140 (MQ=255)

                                                        cAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATtaataa                                                                                <  1:8203315/140‑1 (MQ=255)

                                                         aaaTGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAg                                                                               >  2:4681679/1‑140 (MQ=255)

                                                            tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                            >  1:859289/1‑140 (MQ=255)

                                                            tGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGt                                                                            >  2:6533470/1‑140 (MQ=255)

                                                              tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTc                                                                          >  2:10575757/1‑140 (MQ=255)

                                                              tagtaCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑ttttttacg                                                                                                                               <  1:11282471/86‑5 (MQ=255)

                                                                 taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                       >  1:1243207/1‑140 (MQ=255)

                                                                 taCGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGt                                                                       >  2:5392405/1‑140 (MQ=255)

                                                                       gggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactga                                                                 >  2:1039439/1‑140 (MQ=255)

                                                                        ggCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtgactgac                                                                >  1:1792677/1‑140 (MQ=255)

                                                                         gCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACg                                                               >  1:5436587/1‑140 (MQ=255)

                                                                              tGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGAc                                                          <  1:2820309/140‑1 (MQ=255)

                                                                              tGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGAc                                                          <  1:11554198/140‑1 (MQ=255)

                                                                                aCCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAg                                                        <  1:10695738/140‑1 (MQ=255)

                                                                                aCCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAg                                                        >  1:3380529/1‑140 (MQ=255)

                                                                                 ccTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgc                                                       >  2:738034/1‑140 (MQ=255)

                                                                                 ccTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgc                                                       >  1:5999630/1‑140 (MQ=255)

                                                                                  cTGGTTTATTGACCGGCGTCTGAACGGC‑AAAATCAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAgcg                                                      >  1:6794971/1‑140 (MQ=255)

                                                                                  cTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcg                                                      <  2:5738903/140‑1 (MQ=255)

                                                                                    ggTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGtga                                                                     <  2:9413681/123‑1 (MQ=255)

                                                                                    ggTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAgcggc                                                    >  1:6237934/1‑140 (MQ=255)

                                                                                     gTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                   <  2:6073324/140‑1 (MQ=255)

                                                                                     gTTGATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAgcggcg                                                   <  1:4149047/140‑1 (MQ=255)

                                                                                         aTTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                               >  2:1182350/1‑140 (MQ=255)

                                                                                         aTTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATc                                               >  1:10961350/1‑140 (MQ=255)

                                                                                           tGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCg                                             <  1:9389735/140‑1 (MQ=255)

                                                                                               cGGCGTCTGAACGGC‑AATAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATACGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  1:8781368/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCCGCGTTTTTTACGCCGTTCTAAAGCGCAAATTTAACAGTCGATCTCCGAGGCCAGTAATAAGCGTGCGGAGCCTGACGACGACAGCGGCGAATCCGTATc                                         >  2:7843316/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCCTTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  1:194090/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGtctc                                         >  1:7191249/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  2:8013611/1‑140 (MQ=255)

                                                                                               cGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATc                                         >  2:6977592/1‑140 (MQ=255)

                                                                                                ggCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATcc                                        >  2:11001647/1‑140 (MQ=255)

                                                                                                  cGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                      >  2:4411905/1‑140 (MQ=255)

                                                                                                  cGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAt                                      >  1:9681767/1‑140 (MQ=255)

                                                                                                    tCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTc                                    >  2:8837712/1‑140 (MQ=255)

                                                                                                       gAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCa                                 <  2:2031800/140‑1 (MQ=255)

                                                                                                         aCGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                               <  1:3539136/140‑1 (MQ=255)

                                                                                                         aCGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACg                               <  1:4244876/140‑1 (MQ=255)

                                                                                                           ggC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTACTAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                             <  2:8307415/140‑1 (MQ=255)

                                                                                                           ggC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGa                             <  1:2459104/140‑1 (MQ=255)

                                                                                                            gC‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGaa                            >  1:4457910/1‑140 (MQ=255)

                                                                                                             c‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                           >  1:8392234/1‑140 (MQ=255)

                                                                                                             c‑AAAAACAAAAGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAg                           >  1:8865979/1‑140 (MQ=255)

                                                                                                                      aaaGCATGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGc                   >  2:10512153/1‑140 (MQ=255)

                                                                                                                       aaGCATGGTGAATCGCCAGAGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAAATAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCg                  <  1:9788831/140‑1 (MQ=255)

                                                                                                                           caggTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCAGTCCCACGGAAGATATTAGCGAAcc              <  2:4795842/138‑1 (MQ=255)

                                                                                                                           aTGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc              <  1:9544121/140‑1 (MQ=255)

                                                                                                                           aTGGTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAAcc              <  1:3774350/140‑1 (MQ=255)

                                                                                                                             tgTGATTCGCCAGCGTTTTTTACGCCTTTATAAATCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGa            <  1:6513072/139‑1 (MQ=255)

                                                                                                                              gTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt           <  2:6767023/140‑1 (MQ=255)

                                                                                                                              gTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt           >  2:6232994/1‑140 (MQ=255)

                                                                                                                              gTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt           <  1:7798690/140‑1 (MQ=255)

                                                                                                                              gTGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGAt           <  2:1115689/140‑1 (MQ=255)

                                                                                                                               tGAATCGCCAGCGTTTTTTACGCCGTTCTAAAGCGCAATTTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTCTCCATTCCCACGGAAGAGATTAGCGAACCGATg          >  2:3504509/1‑140 (MQ=255)

                                                                                                                               tGAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATCTTAGCGAACCGATg          >  2:3679542/1‑140 (MQ=255)

                                                                                                                                gAATCGCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGt         <  1:2986746/140‑1 (MQ=255)

                                                                                                                                    cGCCAGCGCTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCAGTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGAGATTAGCGAACCGATGTTtca     >  2:2713328/1‑140 (MQ=255)

                                                                                                                                     gCCAGCGTTTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTtcat    <  2:7650895/140‑1 (MQ=255)

AACGACAGCGAACCGTGGGCCTGAGAAGCGGCAACACAGGCGTAGCATACAGTTGGCAAATGTAGTACGGGGGGCATATGACCTGGTTTATTGACCGGCGTCTGAACGGC‑AAAAACAAAAGCATGGTGAATCGCCAGCG‑TTTTTACGCCGTTATAAAGCGCAAATTAAACAGTCGATCTCCGAGGCCATTAATAAGCGTTCGGTGACTGACGTCGACAGCGGCGAATCCGTATCCATTCCCACGGAAGATATTAGCGAACCGATGTTTCATCA  >  NZ_CP025268/1873693‑1873965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`