Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP025268 2,466,923 (C)8→9 intergenic (+242/‑123) fadL → / → CXP41_RS12955 long‑chain fatty acid transporter FadL/protein YfdF

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,466,9151.C93.0% 760.7 / 52.0 245intergenic (+234/‑131)fadL/CXP41_RS12955long‑chain fatty acid transporter FadL/protein YfdF
Reads supporting (aligned to +/- strand):  ref base . (1/16);  new base C (24/202);  total (25/220)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCA  >  NZ_CP025268/2466778‑2467046
                                                                                                                                           |                                                                                                                                       
ttGCAATCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTTTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTAAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑cc                                                                                                                                      <  1:9111765/140‑1 (MQ=255)
ttGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAAGAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑cc                                                                                                                                      <  2:1296200/140‑1 (MQ=255)
 tGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc                                                                                                                                     <  2:5459890/140‑1 (MQ=255)
   cAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc                                                                                                                                  <  2:8049441/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCACATCAATAAGCGGGATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑c                                                                                                                              <  2:444103/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑c                                                                                                                              <  2:1464722/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑c                                                                                                                              <  1:11179320/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑c                                                                                                                              <  2:6204032/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑c                                                                                                                              <  1:3500510/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑c                                                                                                                              <  1:6516513/140‑1 (MQ=255)
     aaCAGCCTGTTGGCAGCTTGCCATTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑c                                                                                                                              <  2:4280352/140‑1 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGATGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTTCTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑ccc                                                                                                                            <  2:2664028/140‑2 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑ccc                                                                                                                            <  1:6748938/140‑2 (MQ=255)
       cAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑ccc                                                                                                                            <  1:5737530/140‑2 (MQ=255)
          ccagTTGCCAGCTTGCCCCTTCAACACAAACAGCGGTATCCACACACAAACCTGAACGGAACGGCAAAATATCACGCGTTCAGCACGTCCCTCCCAAAGAGACGACTTTACTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑ccctgg                                                                                                                         <  2:4570775/137‑5 (MQ=255)
          ccTGTTGGCAGCTTTCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGTCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑ccctgg                                                                                                                         <  2:8858091/140‑5 (MQ=255)
          ccTGTTGGCAGCTTGCCCTTTCAAATCAATACGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑CCC‑CC‑C‑CCCggg                                                                                                                         <  2:405011/140‑2 (MQ=255)
          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑ccctgg                                                                                                                         <  1:10219359/140‑5 (MQ=255)
          ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑ccctgg                                                                                                                         <  2:1574324/140‑5 (MQ=255)
              ttGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTAt                                                                                                                      <  1:11586092/140‑1 (MQ=255)
                ggCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtg                                                                                                                    <  2:6033044/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  1:7781094/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATCAGCGGTATCCACAGACAAACCGGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  2:3760166/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGTTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  2:5674127/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  1:2898192/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  1:6968701/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  1:6308240/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  1:8182198/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  2:170841/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  1:1907015/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  1:11618564/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  2:6561948/140‑1 (MQ=255)
                  cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTATGCATTTCCAGTAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  2:4900838/140‑1 (MQ=255)
                  cAGCTTGCCCTTACAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTATCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  2:10132526/140‑1 (MQ=255)
                  cAGCTTGCCCTATCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑AAT‑‑CCC‑CCAC‑CCGGGCTATtgtg                                                                                                                  <  2:2823541/140‑1 (MQ=255)
                   aGCTTGCCCTTTCAAATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTCCCCC‑CC‑C‑CCGGGCTATtgtg                                                                                                                  <  1:9868795/140‑1 (MQ=255)
                    tctgtcccTTTCAACTCAAGAAGCGGTATCCACAGACAAACCTGAAGGGTAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTgcg                                                                                                                <  2:8189595/135‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATCAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTgcg                                                                                                                <  1:10368085/140‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACATTCCTGTAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCTTTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTgcg                                                                                                                <  2:9588861/140‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTgcg                                                                                                                <  1:2265491/140‑1 (MQ=255)
                    gCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTgcg                                                                                                                >  2:8665493/1‑140 (MQ=255)
                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  >  2:9158063/1‑134 (MQ=255)
                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATtgtg                                                                                                                  <  1:9158063/134‑1 (MQ=255)
                        gCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCa                                                                                                            <  1:8896701/140‑1 (MQ=255)
                           cTTTCAAATCAATAAGCGGTATCCACAGACCAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTATGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATac                                                                                                         <  2:2312174/140‑1 (MQ=255)
                            tttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATaca                                                                                                        <  1:9740501/140‑1 (MQ=255)
                              tCAAATCAATAAGCGGTATCCACATACAAACCTGTAGGGATAGTCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTAATTTTCTGAATTTCCTGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACt                                                                                                      <  2:1992480/140‑1 (MQ=255)
                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACt                                                                                                      <  1:9337481/140‑1 (MQ=255)
                              tCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACt                                                                                                      <  2:10881400/140‑1 (MQ=255)
                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTc                                                                                                     <  1:129677/140‑1 (MQ=255)
                               cAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑ccc‑cc‑c‑c                                                                                                                              <  2:9295782/114‑1 (MQ=255)
                                  aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCaaa                                                                                                  <  1:11317975/140‑1 (MQ=255)
                                  aTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCGGGCTCTTGTGCGCTCATACCCTCaaa                                                                                                  >  1:8966234/1‑140 (MQ=255)
                                     aaTAAGCGTTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                               <  1:10807668/140‑1 (MQ=255)
                                     aaTAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTa                                                                                               <  1:7472263/140‑1 (MQ=255)
                                      aTAAGCGTTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGAGTTCTGCTCGTCATTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTaa                                                                                              <  2:7251272/140‑1 (MQ=255)
                                         aGCGGTATCCACATACATACCTTATGGGAAATGCTAATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                           <  2:11552614/140‑1 (MQ=255)
                                          gctgTATCCAAAGACAAACCTGAAGGGAAAGGCATAATTTCAGGCGTTCTGCTCGTCCTTCACAACGAGTTTACTTTTCTGCTTTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATTCACTCAAATTCAatat                                                                                          <  2:7903809/137‑1 (MQ=255)
                                          gCGTTATCCACAGACAAACATGAAGGTAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAATAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  2:10478191/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGGTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          >  1:8293794/1‑140 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  1:7200881/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  2:8293794/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  2:7431070/140‑1 (MQ=255)
                                          gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                          <  1:25792/140‑1 (MQ=255)
                                          gCGGTATCCACAGAAAAAACTGAAGGTAAAGTCATATGTTCAGGCGTTCTGCTCGTCCTTCTCATAGAGTTGTCTTTTATGCATTTACAGGAT‑ACT‑‑CCC‑CC‑CACCTGGCTATTGGGCGCTCATACACTCAAATTAAAGAt                                                                                          <  2:8348242/140‑1 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     >  2:7514126/1‑140 (MQ=255)
                                               aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     <  1:11049981/140‑1 (MQ=255)
                                               aTCCACAGAAAAACCTGAAGGGAAAGTCATATTTTCAGGCGTTATGCTCGTCCTTCTCAAAGAGTTTACTTTTATGCATTTACAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     <  2:7851482/140‑1 (MQ=255)
                                                    caGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                                                                <  2:3427664/140‑1 (MQ=255)
                                                          aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCGATTGTGCGCTCATACACTCCAATTAAAGATAGGTTCTAAATAAATg                                                                          >  1:4266849/1‑140 (MQ=255)
                                                           aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCGATTGTGCGCTCATACACTCAAATTAAAGATAGGGTCTAAATAAATGa                                                                         >  1:5560656/1‑140 (MQ=255)
                                                            ccTGAAGGGAAAGGCATTTTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                        <  2:1128365/140‑1 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                        <  1:1663848/140‑1 (MQ=255)
                                                            ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAg                                                                        <  1:4748577/140‑1 (MQ=255)
                                                               gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                                                                    <  1:2778343/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCACATTAAAGATAGGTTCTAAATACATGAGCGTTTTTTGa                                                              <  2:2396556/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:1599906/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:3165444/140‑1 (MQ=255)
                                                                      aaGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:2398627/140‑1 (MQ=255)
                                                                        tgCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCACGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCTCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                            <  2:7025687/139‑1 (MQ=255)
                                                                        ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATa                                                            <  2:9419199/140‑1 (MQ=255)
                                                                         gCATATTTTCAGGCGTTCTGCGCTTCCTTCACAACGAGTTTACTTTTCTGCATTTCCAGTATCATC‑‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAACGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                                                           <  1:10429837/140‑1 (MQ=255)
                                                                          cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                          <  1:3905923/140‑1 (MQ=255)
                                                                          cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGt                                                          <  2:2179730/140‑1 (MQ=255)
                                                                             atTTTCAGGCGTTCTGCTCGTCGTTCTCTAAGAGTTTACTTTTCTGCATTTCCTGGAT‑TCT‑‑CCCGCC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGTTAGTCTa                                                       <  1:3966417/140‑1 (MQ=255)
                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                       <  2:10242870/140‑1 (MQ=255)
                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                       <  2:708860/140‑1 (MQ=255)
                                                                             atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                       <  1:4641360/140‑1 (MQ=255)
                                                                             atTTTCAGGCGTTCTGCTCGTCCTTATCAAAGAGTTTCCTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTa                                                       <  1:10674192/140‑1 (MQ=255)
                                                                                   aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTCTTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                 <  1:4140552/140‑1 (MQ=255)
                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  2:2958425/140‑1 (MQ=255)
                                                                                    ggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                <  1:79435/140‑1 (MQ=255)
                                                                                       ggtCTGCTCGTCCTTCTCAAAGAGTGTACTTGTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg                                             <  2:7720673/138‑1 (MQ=255)
                                                                                         tCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa                                           <  1:8038085/140‑1 (MQ=255)
                                                                                             cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                       <  1:8720636/140‑1 (MQ=255)
                                                                                                 tCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGtt                                                                                     >  1:7338761/1‑90 (MQ=255)
                                                                                                  ccTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:7839574/112‑1 (MQ=255)
                                                                                                      ctcAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa                              <  1:7644708/140‑1 (MQ=255)
                                                                                                      ctcAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa                              <  1:10491254/140‑1 (MQ=255)
                                                                                                                 tACTTTTCTGCATTTCCAGGAT‑ACTC‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGa                                                              <  1:5247783/97‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                       
TTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTTCCAGGAT‑ACT‑‑CCC‑CC‑C‑CCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAATCAATCAAAATGGAATAAAATCA  >  NZ_CP025268/2466778‑2467046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑