BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	320,615	(G)_6→7	intergenic (+482/‑44)	rclR → / → ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	320,609	1	.	G	98.1%	3064.8 / 41.7	891	intergenic (+476/‑50)	rclR/ykgE	reactive chlorine‑specific transcriptional regulator RclR/heterodisulfide reductase‑related iron‑sulfur binding cluster
Reads supporting (aligned to +/- strand): ref base . (7/10); new base G (271/603); total (278/613)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 944 total aligned reads displayed.

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGT‑GGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

 gCGATGCCGAAAGAAATAAAATTAGTTAGTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGT‑g                                                                                                                                         <  2:9712226/140‑1 (MQ=255)

  cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGT‑gg                                                                                                                                        <  2:2778895/140‑1 (MQ=255)

  cGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGT‑gg                                                                                                                                        >  2:11160973/1‑140 (MQ=255)

  cGATGCCGAAAGAAATAAAATGAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGT‑gg                                                                                                                                        <  2:1264537/140‑1 (MQ=255)

    aTGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATAGTTATTGATGATTGATCCCC‑CGGT‑ggg‑g                                                                                                                                     >  2:4379755/1‑140 (MQ=255)

               aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCg                                                                                                                          >  1:5033938/1‑140 (MQ=255)

               aaTAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCg                                                                                                                          >  2:457143/1‑140 (MQ=255)

                 tAAAATTAGTTCTTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCCTACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGAGCCCC‑CGGTGGGG‑GGG‑AAACATCggg                                                                                                                        <  2:9114948/140‑1 (MQ=255)

                       tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATaaa                                                                                                                  <  2:6062505/140‑1 (MQ=255)

                       tAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATaaa                                                                                                                  <  2:3708519/140‑1 (MQ=255)

                        aGTTATTGCATTTGACGTTTGGATGAAAGTTTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAAt                                                                                                                 >  1:2820811/1‑140 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCa                                                                                                            <  1:11228733/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCa                                                                                                            <  1:2488660/140‑1 (MQ=255)

                             ttGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCa                                                                                                            <  2:303805/140‑1 (MQ=255)

                              tGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCAt                                                                                                           >  1:9675194/1‑140 (MQ=255)

                               gCATTTGACGTTTGGATGAAATATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  1:2123590/140‑1 (MQ=255)

                               gCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATg                                                                                                          <  1:6572361/140‑1 (MQ=255)

                                  tttGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAg                                                                                                       <  2:1711116/140‑1 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:3174330/140‑1 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:4402287/140‑1 (MQ=255)

                                     gACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGT‑GGG‑GGGTAAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  2:1006817/140‑1 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAAt                                                                                                   <  1:2688573/140‑1 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAAt                                                                                                   <  2:10259357/140‑1 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAAt                                                                                                   <  2:11292850/140‑1 (MQ=255)

                                      aCGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAAt                                                                                                   <  2:8477565/140‑1 (MQ=255)

                                       cGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATg                                                                                                  >  2:5510641/1‑140 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  1:3721278/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  2:10774944/140‑1 (MQ=255)

                                        gTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATgg                                                                                                 <  2:5033938/140‑1 (MQ=255)

                                          ttGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAg                                                                                               >  2:1559976/1‑140 (MQ=255)

                                           tGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGt                                                                                              >  2:1988840/1‑140 (MQ=255)

                                            ggATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa                                                                                             <  1:5751712/140‑1 (MQ=255)

                                            ggATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTa                                                                                             <  1:8747039/140‑1 (MQ=255)

                                                gAAAGTTTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGACATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAg                                                                                         <  2:2091598/140‑1 (MQ=255)

                                                        aTCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAAt                                                                                 >  2:1820280/1‑140 (MQ=255)

                                                              tGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAt                                                                           >  2:4000862/1‑140 (MQ=255)

                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                                                                          <  2:1448440/140‑1 (MQ=255)

                                                               gTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAAtt                                                                          <  2:530948/140‑1 (MQ=255)

                                                                 cATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTc                                                                        <  2:2470699/140‑1 (MQ=255)

                                                                  aTACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCt                                                                       <  1:5246099/140‑1 (MQ=255)

                                                                   tACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    >  2:9194938/1‑110 (MQ=255)

                                                                   tACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCtt                                                                      >  2:3420090/1‑140 (MQ=255)

                                                                   tACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCtt                                                                      >  1:1816079/1‑140 (MQ=255)

                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTCTTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  2:8672572/140‑1 (MQ=255)

                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  1:2975991/140‑1 (MQ=255)

                                                                    aCAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCttt                                                                     <  2:9539435/140‑1 (MQ=255)

                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  2:7720182/140‑1 (MQ=255)

                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  2:6617749/140‑1 (MQ=255)

                                                                      aaaTGAGGGGTGGTATGTTGCTAGCCAAATAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGt                                                                   <  2:8017105/140‑1 (MQ=255)

                                                                       aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                  <  2:11482070/140‑1 (MQ=255)

                                                                       aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                  <  1:7142252/140‑1 (MQ=255)

                                                                       aaTGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTc                                                                  <  2:5676141/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCATTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:468900/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  2:2588760/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:9228217/140‑1 (MQ=255)

                                                                         tGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:7738226/140‑1 (MQ=255)

                                                                          gAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAcc                                                               >  1:5595533/1‑140 (MQ=255)

                                                                           aGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt                                                              <  2:10352030/140‑1 (MQ=255)

                                                                           aGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCt                                                              <  1:1356471/140‑1 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTaa                                                                                                    <  1:1630320/100‑1 (MQ=255)

                                                                             gggTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGt                                                            >  1:2316196/1‑140 (MQ=255)

                                                                              ggtggaATGATCCTACCAAATTAAAAAAGAACGCCATATAAATTGATGATAGATCCCC‑CATT‑GGGCGTG‑AAACATCGCGCATAAATCGGCATGAAGTAATGCAGTATTATTTATGAAAGACAATTTCTTTGACaaattta                                                           >  2:10624584/1‑134 (MQ=255)

                                                                                tggtATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTAtt                                                         >  1:8076484/1‑140 (MQ=255)

                                                                                     tGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGa                                                    <  1:7440590/140‑1 (MQ=255)

                                                                                     tGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGa                                                    <  2:4908024/140‑1 (MQ=255)

                                                                                       ttGCTAGCCAATTAAAAAAGAACGCCATCTTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACg                                                  <  2:6887137/140‑1 (MQ=255)

                                                                                       ttGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACg                                                  >  2:7574724/1‑140 (MQ=255)

                                                                                         gcgaGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                <  1:6462055/137‑1 (MQ=255)

                                                                                         gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                <  1:6004342/140‑1 (MQ=255)

                                                                                         gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                <  1:10799258/140‑1 (MQ=255)

                                                                                         gCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGcc                                                <  2:7116344/140‑1 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGt                                                                                                                                           <  2:473519/50‑1 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:8983621/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:1987660/1‑140 (MQ=255)

                                                                                          cTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGccc                                               >  1:7325947/1‑140 (MQ=255)

                                                                                            aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             >  2:5873795/1‑140 (MQ=255)

                                                                                            aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             <  1:11047013/140‑1 (MQ=255)

                                                                                            aGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTg                                             <  1:1052879/140‑1 (MQ=255)

                                                                                             gaaaaTAAAAAAACAAAGCCAAATTTATAGATGATAGATCCCCATGAT‑TGC‑GGT‑AAACATCTGTCATAAATGGTAATGAATTAAAGAAGTATTAGTTGTTAAAGTAAATTTCTTTTTCACCTGTATTGGTGACGCCCTGa                                            >  2:4231810/4‑140 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAAt                                                                                                   <  1:7571091/85‑1 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                >  2:2320451/1‑120 (MQ=255)

                                                                                             gCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGa                                            >  2:10937433/1‑140 (MQ=255)

                                                                                                 aTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCAc                                                                <  1:10338784/116‑1 (MQ=255)

                                                                                                 aTTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATc                                        <  2:313609/140‑1 (MQ=255)

                                                                                                      aaaaGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:3087505/140‑1 (MQ=255)

                                                                                                      aaaaGAACGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGaa                                   <  1:10670817/140‑1 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  1:6799172/140‑1 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACg                            <  2:8231074/140‑1 (MQ=255)

                                                                                                             cGCCATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTGTGTCACCGGTATTGGTGACGCCCTGAATTCAAGAATGGCACg                            >  2:7390618/1‑140 (MQ=255)

                                                                                                                cATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgaga                         >  1:4076164/1‑140 (MQ=255)

                                                                                                                cATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgaga                         <  2:8906148/140‑1 (MQ=255)

                                                                                                                cATATTTATTGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACgaga                         <  2:6232140/140‑1 (MQ=255)

                                                                                                                  tatttattGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACt                       <  1:5387670/140‑1 (MQ=255)

                                                                                                                   atttattGATGATTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTc                      <  2:6438924/140‑1 (MQ=255)

                                                                                                                          gatgatTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGa                                                                                   <  1:8474690/72‑1 (MQ=255)

                                                                                                                          gatgatTGATCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGtgctg               >  1:4827564/1‑140 (MQ=255)

                                                                                                                              attgatCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  1:11624771/1‑140 (MQ=255)

                                                                                                                              attgatCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTAc           >  1:10024344/1‑140 (MQ=255)

                                                                                                                                 gatCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTgg        <  1:6347264/140‑1 (MQ=255)

                                                                                                                                 gatCCCC‑CGGTGGGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTgg        <  1:9467399/140‑1 (MQ=255)

AGCGATGCCGAAAGAAATAAAATTAGTTATTGCATTTGACGTTTGGATGAAAGATTATCATTTGTCATACAAATGAGGGGTGGTATGTTGCTAGCCAATTAAAAAAGAACGCCATATTTATTGATGATTGATCCCC‑CGGT‑GGG‑GGG‑AAACATCGGGCATAAATGGGCATGAAGTAATGGAGTATTAGTTGTGAATGTCAATTTCTTTGTCACCTGTATTGGTGACGCCCTGAAATCAAGAATGGCACGAGACTCCGTGCTGCTACTGGAAAAAC  >  NZ_CP025268/320470‑320743

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`