BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	3,239,247	(T)_6→7	intergenic (+264/‑18)	ygjR → / → alx	Gfo/Idh/MocA family oxidoreductase/TerC family membrane protein Alx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	3,239,241	1	.	T	97.7%	1949.7 / 30.6	570	intergenic (+258/‑24)	ygjR/alx	Gfo/Idh/MocA family oxidoreductase/TerC family membrane protein Alx
Reads supporting (aligned to +/- strand): ref base . (8/5); new base T (212/337); total (221/349)
Fisher's exact test for biased strand distribution p-value = 1.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.60e-01

Only 100 of 650 total aligned reads displayed.

TGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑T‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACC  >  NZ_CP025268/3239102‑3239375

tgtgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑g                                                                                                                                            >  1:10019289/1‑140 (MQ=255)

tgtgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑g                                                                                                                                            <  1:3457471/140‑1 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t                                                                                                                                        >  1:7434027/1‑140 (MQ=255)

  tgtgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t                                                                                                                                        >  1:11252947/1‑140 (MQ=255)

    tgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑tt                                                                                                                                     >  2:3239093/1‑140 (MQ=255)

    tgAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑tt                                                                                                                                     >  2:9398654/1‑140 (MQ=255)

     gAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑ttt                                                                                                                                    >  1:9098304/1‑140 (MQ=255)

       aaaaaCACATCCGGTCACCGGGCAACCCGAACGGAAGACGCAGACGTATTCCTTTTTTGGTGGAAGGGAGACCTTGCCGGAAGGCGAGGTCTATGCATACAAAGCCGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑ttttt                                                                                                                                  <  2:8148521/140‑2 (MQ=255)

       aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑ttttt                                                                                                                                  <  1:5914333/140‑2 (MQ=255)

       aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑ttttt                                                                                                                                  <  1:4865508/140‑2 (MQ=255)

       aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑ttttt                                                                                                                                  <  2:10724758/140‑2 (MQ=255)

       aaaaaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑ttttt                                                                                                                                  <  1:2162397/140‑2 (MQ=255)

         aaaCACATCCGGTCACCGGGCACCCCGCACGGAATACGCAGACGTAGTCCTTTTTTGGTGTACGTGAGACCTGGCCGGAAGGCGAGGTCTATGCATAAAACGCCGCGGCTGCCGTCTGCCGACGTTGGCC‑G‑t‑t‑tttttat                                                                                                                                <  2:1570948/140‑4 (MQ=255)

          aaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑tttttatg                                                                                                                               >  1:1720732/1‑136 (MQ=255)

          aaCACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑t‑t‑tttttatg                                                                                                                               >  1:6801084/1‑136 (MQ=255)

            cacaTCCGGTCCCCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTGTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑Atgtg                                                                                                                             >  2:9564616/1‑140 (MQ=255)

                 ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAgg                                                                                                                        <  1:7907759/140‑1 (MQ=255)

                 ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAgg                                                                                                                        <  2:5964063/140‑1 (MQ=255)

                 ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAgg                                                                                                                        <  1:9286064/140‑1 (MQ=255)

                 ccGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAgg                                                                                                                        <  1:11075992/140‑1 (MQ=255)

                    gTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTGTTTTGGTGTGAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAAc                                                                                                                     >  1:10842514/1‑140 (MQ=255)

                     tCACCGGGCACCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAACAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACt                                                                                                                    <  1:1633008/140‑1 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACt                                                                                                                    <  1:8649460/140‑1 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACt                                                                                                                    <  1:7314578/140‑1 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACt                                                                                                                    <  1:6256124/140‑1 (MQ=255)

                     tCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCATTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACt                                                                                                                    <  2:11626353/140‑1 (MQ=255)

                     cccccGGGCAACCCCAAAGGACTACCCAGACTTATTCCTTTTTTGTTGTAAGTGAGACCTGTCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACt                                                                                                                    <  2:10830181/137‑1 (MQ=255)

                      cACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACtt                                                                                                                   >  1:4175701/1‑140 (MQ=255)

                       aCCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTc                                                                                                                  <  2:1380210/140‑1 (MQ=255)

                        ccGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCt                                                                                                                 <  2:3594835/140‑1 (MQ=255)

                         cGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTa                                                                                                                >  1:10384284/1‑140 (MQ=255)

                           ggCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATg                                                                                                              >  1:10248520/1‑140 (MQ=255)

                            gCAACCCGAACGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCAGAAAAAGCAGCGGCTGACGTCGTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGCAAGGAACTTCTATGa                                                                                                             <  2:11277015/140‑1 (MQ=255)

                               aCCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATa                                                                                                          >  1:8918232/1‑140 (MQ=255)

                                cccGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATAc                                                                                                         <  2:7766828/140‑1 (MQ=255)

                                   gAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGt                                                                                                      <  2:5620494/140‑1 (MQ=255)

                                          aTACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACg                                                                                               <  1:7117947/140‑1 (MQ=255)

                                           tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                                                                                              <  1:5800717/140‑1 (MQ=255)

                                           tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGc                                                                                              <  2:4339485/140‑1 (MQ=255)

                                           tACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTATATGAANACTGTCGGCACGc                                                                                              <  1:9975763/140‑1 (MQ=255)

                                             cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            >  2:2281411/1‑140 (MQ=255)

                                             cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  2:4879819/140‑1 (MQ=255)

                                             cGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            <  1:3958564/140‑1 (MQ=255)

                                             cGCAGACGTATTCCTGTTTTGTTGGAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCg                                                                                            >  2:9780735/1‑140 (MQ=255)

                                              gCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGt                                                                                           <  1:2829348/140‑1 (MQ=255)

                                                  aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCTGTTGCt                                                                                       >  2:369854/1‑140 (MQ=255)

                                                  aCGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCt                                                                                       >  2:8491420/1‑140 (MQ=255)

                                                     tATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATg                                                                                    >  2:7625071/1‑140 (MQ=255)

                                                            tttttGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGa                                                                             <  1:5523602/140‑1 (MQ=255)

                                                                   tgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtg                                                                      <  1:9397915/140‑1 (MQ=255)

                                                                   tgtAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtg                                                                      <  1:8019961/140‑1 (MQ=255)

                                                                          gagaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCtgttgttgt                                                               <  1:9699796/140‑1 (MQ=255)

                                                                            gaCCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCg                                                             <  1:11463167/140‑1 (MQ=255)

                                                                               cTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCa                                                          <  1:852103/140‑1 (MQ=255)

                                                                                 tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                        >  2:3932925/1‑140 (MQ=255)

                                                                                 tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                        >  1:5256371/1‑140 (MQ=255)

                                                                                 tGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCAtt                                                        >  1:8385477/1‑140 (MQ=255)

                                                                                  gCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTGGGCC‑G‑T‑TGTTTT‑ATGTGTACGGAACTTCTCTGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTa                                                       >  2:8684686/1‑140 (MQ=255)

                                                                                      gAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt                                                   <  1:3087527/140‑1 (MQ=255)

                                                                                      gAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt                                                   <  2:8852829/140‑1 (MQ=255)

                                                                                      gAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCt                                                   >  1:3231089/1‑140 (MQ=255)

                                                                                       aaGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTg                                                  >  2:7129327/1‑140 (MQ=255)

                                                                                         ggCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGc                                                <  1:2467472/140‑1 (MQ=255)

                                                                                           cGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTa                                              <  2:9911727/140‑1 (MQ=255)

                                                                                               gTCTATGCATACAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGCACTTCTATGAATACTGGCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGa                                          <  2:8026565/140‑1 (MQ=255)

                                                                                                tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                         <  2:5168229/140‑1 (MQ=255)

                                                                                                tCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAc                                         <  2:3465584/140‑1 (MQ=255)

                                                                                                 cTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGAcc                                        <  2:6115536/140‑1 (MQ=255)

                                                                                                    tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgt                                     <  1:4525352/140‑1 (MQ=255)

                                                                                                    tGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGATGTTGTCGCCATTATGCTGGCTATCGACCtgt                                     <  2:4677618/140‑1 (MQ=255)

                                                                                                       aTAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCtgttgt                                  <  2:11173600/140‑1 (MQ=255)

                                                                                                         aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                                >  1:10599014/1‑140 (MQ=255)

                                                                                                         aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                                >  1:4374792/1‑140 (MQ=255)

                                                                                                         aaaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGttgttg                                >  2:10536817/1‑140 (MQ=255)

                                                                                                          agcagcagcGGCGGGCGTCGTCCGTCGGTGGCCGG‑T‑T‑GTGT‑AGGTGTAAGGCACGTCTCTGCAGACTGTCGGCACGCCGTTGCTCGGGGGCGGCTTCGCTGTTGTTGTCGCCAGTCGGCTGGCGAGCGACCTGTGGTGGc                               >  2:5314796/4‑140 (MQ=255)

                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGACCTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  2:5519612/1‑140 (MQ=255)

                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATTCTGGCTATCGACCTGTTGTTGc                               >  2:6968040/1‑140 (MQ=255)

                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  2:5062252/1‑140 (MQ=255)

                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  2:3015625/1‑140 (MQ=255)

                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  1:9531193/1‑140 (MQ=255)

                                                                                                          aaaaGCAGCGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTAGGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGc                               >  2:7430394/1‑140 (MQ=255)

                                                                                                              gcagcGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                           >  1:1064087/1‑140 (MQ=255)

                                                                                                              gcagcGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAggg                           >  2:2764048/1‑140 (MQ=255)

                                                                                                                 gcGGCTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGcg                        <  1:1520177/140‑1 (MQ=255)

                                                                                                                     cTGACGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAACACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATTCTGGCTATCGACCTGTTGTTGCAGGGGcgtcgt                    <  1:2661338/140‑1 (MQ=255)

                                                                                                                      tGACGTCTGCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTg                   <  1:8271848/140‑1 (MQ=255)

                                                                                                                       gACGTCGTCCGACGTTGGCC‑G‑TGT‑GTTT‑ATGTGTAAGGAACTTCGATGAAGACTGTCGGCACGCCGTGGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCGATCGACCTGTTGTGGCAGGGGCGTCGTgg                  <  2:10158188/140‑1 (MQ=255)

                                                                                                                         cGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTgggg                <  2:1950400/140‑1 (MQ=255)

                                                                                                                         cGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTgggg                <  1:2536697/140‑1 (MQ=255)

                                                                                                                         cGTCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTgggg                <  2:9002386/140‑1 (MQ=255)

                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGCACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  1:6595862/140‑1 (MQ=255)

                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  2:9135539/140‑1 (MQ=255)

                                                                                                                           tCTTCCGACGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  1:3765291/140‑1 (MQ=255)

                                                                                                                           tCTTCCGACGTTGGCC‑G‑T‑TATTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGca              <  1:3621347/140‑1 (MQ=255)

                                                                                                                                   cGTTGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat      <  2:2573171/140‑1 (MQ=255)

                                                                                                                                   cGTGGGCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccat      <  2:644084/140‑1 (MQ=255)

                                                                                                                                       ggCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  <  1:9855497/140‑1 (MQ=255)

                                                                                                                                       ggCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  >  2:6741379/1‑140 (MQ=255)

                                                                                                                                       ggCC‑GTT‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTCGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  <  1:1200920/140‑1 (MQ=255)

                                                                                                                                       ggCC‑G‑T‑T‑TTTTGATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGccatgacc  >  2:1682476/1‑140 (MQ=255)

TGTGTGAAAAAACACATCCGGTCACCGGGCAACCCGAAAGGAATACGCAGACGTATTCCTTTTTTGTTGTAAGTGAGACCTTGCCGGAAGGCGAGGTCTATGCATAAAAAGCAGCGGCTGACGTCTTCCGACGTTGGCC‑G‑T‑T‑TTTT‑ATGTGTAAGGAACTTCTATGAATACTGTCGGCACGCCGTTGCTATGGGGCGGATTCGCTGTTGTTGTCGCCATTATGCTGGCTATCGACCTGTTGTTGCAGGGGCGTCGTGGGGCACATGCCATGACC  >  NZ_CP025268/3239102‑3239375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`