Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252682,233,2260CA20.9% 306.5 / 20.4 187intergenic (+126/+247)yohP/dusCsmall membrane protein YohP/tRNA dihydrouridine(16) synthase DusC
Reads supporting (aligned to +/- strand):  ref base C (82/66);  new base A (39/0);  total (121/66)
Fisher's exact test for biased strand distribution p-value = 5.53e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 198 total aligned reads displayed.

GATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGG  >  NZ_CP025268/2233088‑2233365
                                                                                                                                            |                                                                                                                                           
gatgatATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCCCTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATcg                                                                                                                                            >  2:8428279/1‑140 (MQ=255)
     tatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATcgccgcc                                                                                                                                       <  2:824242/140‑1 (MQ=255)
     tatTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATcgccgcc                                                                                                                                       <  2:8916485/140‑1 (MQ=255)
      atTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATcgccgccg                                                                                                                                      <  1:615985/140‑1 (MQ=255)
        tttAAAATTAATTAATGTCATCAGGTCCGACAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCgccgccgcc                                                                                                                                    <  2:10228800/140‑1 (MQ=255)
        tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCgccgccgcc                                                                                                                                    >  2:2899701/1‑140 (MQ=255)
        tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCgccgccgcc                                                                                                                                    <  1:5656859/140‑1 (MQ=255)
        tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCgccgccccc                                                                                                                                    >  2:8373585/1‑140 (MQ=255)
        tttAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCCCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCgccgccgcc                                                                                                                                    <  2:1760280/140‑1 (MQ=255)
             aattaattAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTg                                                                                                                               <  1:2797353/140‑1 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGCGGCCGGGGGGGGCCCGACCCCCCCCCCCCCCCCCACCCCCggcgg                                                                                                                   >  1:5300661/1‑137 (MQ=255)
                           atcaGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCCGGGGGGG‑CCCGACGCCGCCGCCCCCCCCCCCCCCGGGctct                                                                                                                 >  1:9476038/1‑140 (MQ=37)
                                 tCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTATGGCGaa                                                                                                           >  2:10203495/1‑140 (MQ=255)
                                   cGAAAATAACGAGACTATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCTGGCGAACa                                                                                                         >  2:11093095/1‑140 (MQ=255)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCCATCGCCGCCGCCCCCTGCACCCCCGGGCTCAGGCGAACa                                                                                                         >  2:6704944/1‑140 (MQ=255)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACa                                                                                                         >  2:5943222/1‑140 (MQ=255)
                                   cGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACa                                                                                                         >  2:5944222/1‑140 (MQ=255)
                                   cGAAAATAACGAGAATATTTCAGTCGCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCAGCACCCCCGGGCACTGGCGcaca                                                                                                         >  2:1960208/1‑140 (MQ=255)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGCGG‑GCCATCGCCGCCGCCCCCCGCCCCCCCCGGCGCAGGCGCACcaa                                                                                                       >  2:3484455/1‑140 (MQ=255)
                                     aaaaTAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGACGGGGGGG‑CCCATCGCCGCCGCCCCCAGCCCCCCCCGGCGCTGGccgccaaa                                                                                                       >  1:634605/1‑133 (MQ=255)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGGGG‑GCAATAGCCCCCGCCCCCAGCACCCCCGGGCTAAGGCGAACaaaaaagc                                                                                                  >  2:8123088/1‑136 (MQ=25)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATcgc                                                                                                  <  2:541106/140‑1 (MQ=255)
                                          aaCGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATagc                                                                                                  >  2:7803286/1‑140 (MQ=255)
                                               gactatTTCAGTCTCTCAGCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGG‑CCAATCGCCCCCGCCCCCAGCCCCCCCCGGCACTGGCGAACAAAATCGCCGCTg                                                                                             >  2:6258960/4‑140 (MQ=21)
                                                   atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                                         <  1:10684746/140‑1 (MQ=255)
                                                   atTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGcgcg                                                                                         <  2:559761/140‑1 (MQ=255)
                                                     ttCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCCAACGCCGCCGCCCCCTGCACCCCCCGGCTCTGGCGCACCAAAACGCCGCAGCGCGGt                                                                                       >  2:8964760/1‑140 (MQ=18)
                                                      tCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTg                                                                                      <  2:8899338/140‑1 (MQ=255)
                                                        aGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCTGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGcc                                                                                    <  1:9250131/140‑1 (MQ=255)
                                                         gTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCTTATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAAGCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGccc                                                                                   <  1:5493636/140‑1 (MQ=255)
                                                          tctctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCAGCACCCCCCGGCGCTGGCGAACAAAATAGCCCCTGCCCGGTGccca                                                                                  >  2:10654269/1‑139 (MQ=18)
                                                            tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGG‑GCAAACGCCGCCGCCCCCTGCACCCCCGCGCGCTTGCGAACAAAATCGCCGCGGCGCGGTGCCCACg                                                                                >  2:7130509/1‑140 (MQ=18)
                                                            tctcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCCATAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCg                                                                                >  2:6421409/1‑140 (MQ=255)
                                                              tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCCAAAGCCCCCCCCCCCCGCACCCCCCCGCAAATGCCAAAAAAAAAACCCCAGCGCGGTGCCCACGGc                                                                              >  1:1814005/1‑140 (MQ=16)
                                                              tcATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATAGCCGCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAATCGCCCCTGCGCGGTGCCCACGGc                                                                              >  2:9441649/1‑140 (MQ=18)
                                                              tcATCCTCTTGCGCCCCTGTCATGTGCATTGCGTCAGAGAAGCACTGGCGCCAGGAGCGC‑GCCCGGGGCGG‑CCAATCGCCGCCGGCCCCAGCCCCCCCCCGCTCGGGCGAACAAAAGCGCCGCTGCGCGGTGCCCGCggg                                                                              >  2:789209/1‑139 (MQ=17)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCt                                                                             <  1:4385335/140‑1 (MQ=255)
                                                               cATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCATGGGGGGG‑CCAAAACCCGCCCCCCCCAACACCCCCCGGCACTGGCGAAAAAAATAACCCCTGCGCGGTGCCCACGGcg                                                                             >  2:4158161/1‑139 (MQ=18)
                                                                 tCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTa                                                                           <  1:1953430/140‑1 (MQ=255)
                                                                    tGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑GCAATCGCCGCCGCCCCCTGCACCCCCCGGCACTGGCGAACAAAAAAGCCGCAGCCCGGTGCCCAAGGCTTATcc                                                                        >  2:10016212/1‑140 (MQ=18)
                                                                      ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCt                                                                      >  2:10641688/1‑140 (MQ=255)
                                                                      ttGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCAATCACCACCCGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCGCTCGGCTTTTCCCt                                                                      >  2:8606772/1‑140 (MQ=18)
                                                                        gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGGGG‑CCCAAACCCCCCCCCCCCCGCACCCCCCCGCGCAGGGGAAAAAAAAAACCCCAGAGCGGGGGCCCCGGCTTATACCCTa                                                                    >  1:11412340/1‑140 (MQ=255)
                                                                        gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCCGGGGGCGG‑GCAAACGCCGCCGCCCCCAGCCCCCCCGGGCGCTGGCGAACAAAATCGCCGCGGCCCGGTGCCCCCGGCAGATcccaga                                                                    >  2:1845843/1‑137 (MQ=18)
                                                                        gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                    <  1:9777986/140‑1 (MQ=255)
                                                                        gcgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTa                                                                    <  1:9283944/140‑1 (MQ=255)
                                                                         cgcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTAc                                                                   <  1:4907113/140‑1 (MQ=255)
                                                                          gcTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAAACGCCCCCCCCCCCAGCCCCCCCCGGCACAGGCGAACAAAAACACCCCGGCGCGGTGGCCACGGCGTATcccaaaag                                                                  >  2:4560917/1‑135 (MQ=18)
                                                                           cTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCGGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACACAATCGCCGCGGCGCGGTGCCCTCGGCGTATCCCTTACgg                                                                 >  2:7499885/1‑140 (MQ=255)
                                                                           cTCCTGTCATGTGCATTGCTTCATATAAGCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCCAACGCCCCCCCCCCCCGCCCCCCCCGGCGCGGGGGCACCAAAAAGCCCCAGGGCGGGGCCCGCCGCGGATCCCCTACgg                                                                 >  2:2143874/1‑140 (MQ=12)
                                                                            tCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGAGGGGGGGG‑GCAAACCCCCCCCCCCCCAGAAACCCCCGGCGCAGGCGAAAAAAAAAGCCCCAGGCCGGGGCCCACGCCTTATCCCATaatgc                                                                >  1:7917391/1‑136 (MQ=255)
                                                                              cTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGCGG‑CCCAACGCCGCCCCCCCCCGCACCCCCCGGCGCAGGCGAACAAAAACACCGCGGCGCGGGGCCCACGGCGTATACCAAAAGGCTa                                                              >  2:1341783/1‑140 (MQ=12)
                                                                                gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GGCGGGGGGGG‑CCCAAACCCGCCCCCCCCTGGCCCCCCCGGGGCTTGCGAACCAAAACGCCGCTGCGCGGGGGCCACGGCGTAACCCATAACGCTAcc                                                            >  2:2598267/1‑140 (MQ=12)
                                                                                gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCGCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAcc                                                            >  2:11614813/1‑140 (MQ=255)
                                                                                gTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATAGCCGCCGCCCCCAGCACCCCCCGGCGAGGGCGCACAAAAACGCCGCGGCGCGGGGCCCGCGGCGTTTCCCATACGGcgaac                                                            >  2:10814906/1‑136 (MQ=17)
                                                                                   aTGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCCCCGCCCCCCGCACCCCCGGGCTCAGGCGAACAAAATCGCCCCTGCGCGGTGCCCACGGCGTATCCCATACGGCGACCggg                                                         >  2:971891/1‑140 (MQ=17)
                                                                                     gtgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTc                                                       <  2:10102090/140‑1 (MQ=255)
                                                                                      tgCATTGCTTCATATCATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCACGGCGTATCACTAACGGCTACCGAGTCg                                                      >  2:7682087/1‑140 (MQ=14)
                                                                                      tgCATTGCTTCATATACTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCGCGGCTTATCCCTTACGGCTACCGGGTCg                                                      >  2:7625390/1‑140 (MQ=255)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCAGCACCCCCGGGCTCAGGCGAACCAAATCGCCGCTGCGCGGTGCCCACGGCGTTTCCCTTACGGCGACCGGGGCg                                                      >  2:11526937/1‑140 (MQ=14)
                                                                                      tgCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAAACGCCGCCCCCCCCAGCACCCCCCGGCTCATGCGAACAAAATCGCCGCTGCGCGGTGCCCACCGCTTATACCTTACGGCTACCCGGTCg                                                      >  2:11305922/1‑140 (MQ=14)
                                                                                        cATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGACGG‑CCCAACGCCGCCGCCCCCAGCACCCCCCGGCTCAGGCGAACAAAAACGCCGCAGCGCGGTGCCCACCGCTTATACCCTACCGCAACCGCGTCggg                                                    >  2:10817851/1‑140 (MQ=14)
                                                                                          ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGGCGCCCCCTGCACCCCCGGGCGCTGGCGAACAAAATCGCCGCTGGGCGGTGGCCTCGGCTGATCCCTTACGGCTACCGGGTCGGgcg                                                  >  2:10950737/1‑140 (MQ=14)
                                                                                          ttGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGgcg                                                  <  1:639762/140‑1 (MQ=255)
                                                                                           tGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAAACGCCCCCGCCCCCAGCACCCCCCGGCTAAGGCGAAAAAAAACGCCCCAGCGCGGTGCCCACGGCTTATACCATAAGGCAACCCGGTCGGgcgc                                                 >  2:4616143/1‑140 (MQ=14)
                                                                                           tGCCTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAAACGCCCCCGCCCCCCGCACCCCCGGGCTATTGCGAACAAAAGACCCCCGTGGCGGTGCCCGCGGCGTAAACCTTACGGCAACCCGGTCGGgcgc                                                 >  2:5562358/1‑140 (MQ=9)
                                                                                            gCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAACTCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGcgcg                                                <  2:8043521/140‑1 (MQ=255)
                                                                                             cTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAAAACCCGCCGCCCCCAGCACCCCCCGGCACAGGCGAACAAAAAACCCCCGGCGCGGTGCCCACGGCTTATACCATAAGGCAACCCGGTCGGGcgcgc                                               >  2:4687820/1‑139 (MQ=9)
                                                                                              ttCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAg                                              >  2:6896798/1‑140 (MQ=40)
                                                                                                cATATAATCACTGGCGCAAGGAGCGCGGCGGGGGGGGG‑CCAAAC‑CCCCCCCCCCCAGCCCCCCCCGGGGCAGGCGACAAAAAAACCCCCAGCGCGGGGCCCACGGCGGATCACGTACCGCGACCCGGGCGGGGGCGCggg                                            >  1:8576982/1‑139 (MQ=255)
                                                                                                 atatAATCACTGGCGCCAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTa                                           <  1:4333327/140‑1 (MQ=38)
                                                                                                    taATCACGGGCGCACGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGACCAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACa                                        <  1:157321/140‑1 (MQ=255)
                                                                                                     aaTCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACAt                                       <  2:611363/140‑1 (MQ=34)
                                                                                                       tCACTGGCGCAAGGAGCGCGGCGGGGGGGGG‑CCAA‑CGCCGCCCCCCCCCGCCCCCCCCGGCGCTGGCGAAAAAAAACGCCGCGGCGCCGGGGCCACGGCGTATCCCATAACGCGACCCGGTAGGGCGCCCGGGAACaaac                                     >  2:7976637/1‑137 (MQ=255)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GGAGGGGGCGG‑CCCAAAGCCGCCCCCCCCAGCCCCCCCCGGCGCTGGCGAACAAAAAAGCCCCTGCGCGGGGCCCACCGCATATACCATAACGCAACCCGGTCGGGCGCGCGGGAACaaac                                     >  2:421898/1‑137 (MQ=255)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCCGGGGGCGG‑CCCATCGCCGCCGCCCCCCGCACCCCCCGGGGCTGGGGAACAAAATAGCCGCTGGGCGGTGCCCTCGGCGTATCCCGTAACGCGACCCGGGCGGGCGCGAGGGAAccacc                                     >  2:573407/1‑136 (MQ=2)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCCGGGGGCGG‑CCAAAATCCGCCCCCCCCAGCACCCCCCGGCTCAGGCGAACAAAAACGCCCCAGCGCGGTGCGCACGGCTGATACCTTACGGCTAACCGGTCGGGGGCGAGGTaaaaaac                                     >  2:495886/1‑135 (MQ=2)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCCAACGCCGCCCCCCCCAGCACCCCCCGGCTCAGGCGAACAAAAACGCCGCTGCGCGGTGCCCTCGGGATATACCATACGGCTACCCGGTCGGGCGCGAGGGAACaaac                                     >  2:3812913/1‑137 (MQ=11)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCAGCACCCCCGGGCACTGGCGAACAAAAACGCCGCTGCGCGGTGCCCTCGGCATATCCCTTACGGCTAACCGGTAGGGCGCGAGGGAACAtac                                     >  2:10696215/1‑138 (MQ=11)
                                                                                                       tCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATAGCCCCCGCCCCCAGCACCCCCCGGCTCTGGCGAACAAAAACGCCGCTGCGCGGTGGCCTCGGCGTATACCATAAGGCAACCCGGTAGGGCGCGAGGTAACCTcc                                     >  2:6359517/1‑140 (MQ=11)
                                                                                                           tGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCCATCGCCGCCGCCCCCGGCACCCCCGGGCTCAGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCGTACCGCAACCGGGTAGGGGGCGCGGGAACATCCCAGt                                 >  2:6781168/1‑140 (MQ=11)
                                                                                                            ggCGCAAGGAGCGC‑GCAGGGGGCGG‑CCCAAAGCCGCCGCCCCCCGCACCCCCCGGCTCTGGGGAAAAAAAAAACCGCTGCGCGGTGCCCACGGCGTATAACGAAACGCAACCCGGTCGGGCGCGCGGTAACATACCAGTa                                >  2:1650920/1‑140 (MQ=2)
                                                                                                             gcgcAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCGTATCCCGTACGGCTACCGGGTCGGGCGCGCGGTAACAGACCTGTaa                               >  2:8751391/1‑140 (MQ=11)
                                                                                                                 aaGGAGCGC‑GCAGGGGGCGG‑CCACTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAAcg                           <  1:6258960/140‑1 (MQ=17)
                                                                                                                    gAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                        <  2:8267600/140‑1 (MQ=32)
                                                                                                                    gAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTCATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACgcgc                        <  1:4748229/140‑1 (MQ=17)
                                                                                                                       cgc‑gcAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                     <  1:8853213/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                     <  1:7216800/140‑1 (MQ=32)
                                                                                                                       cgc‑gcAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCt                     <  2:5636464/140‑1 (MQ=32)
                                                                                                                             aGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGcc                <  1:10646332/140‑1 (MQ=32)
                                                                                                                                 ggcgt‑cCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTAGCGGGTCGGGCGCGAGGTAACATCCCTGTATAACGCGCCCTCAGCCcaca            <  1:748512/140‑1 (MQ=12)
                                                                                                                                 ggcgg‑cCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCcaca            <  2:3902261/140‑1 (MQ=32)
                                                                                                                                   cgg‑cCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATc          <  1:3484455/140‑1 (MQ=32)
                                                                                                                                   cgg‑cCAATCGCCGCCGCCCCCTGCACCCCCCGGCTCTGGCGAACAAAATCGCCCCTGCGCGGTGCCCTCGGCGTATCCCTTACGGCTACCCGGTCGGGCGCGAGGTAACAACCCAGTAAAACGCgcccacagcccacaac          >  2:4371928/1‑138 (MQ=11)
                                                                                                                                    gg‑cCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATcc         <  2:1496097/140‑1 (MQ=32)
                                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTACCATCCCTGTAAAACGCGCCCTCAGCCCACAGCCAtgtg    >  1:2289632/1‑140 (MQ=17)
                                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCAtgtg    >  1:10451785/1‑140 (MQ=32)
                                                                                                                                          aTCGCCGCCGCCCCCTGCACCCCCGGGCACTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGCCTACCGGGTCGGGCGCGAGGTAACAACCCTGTAAATCGCGCCCTCAGCCCACATCCAtgag    >  2:1847864/1‑138 (MQ=11)
                                                                                                                                           tCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAATCGCGCCCTCAGCCCACATCCATGTgg   >  1:1496661/1‑140 (MQ=17)
                                                                                                                                            cgccgccgcCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTggg  >  1:7840540/1‑140 (MQ=32)
                                                                                                                                            |                                                                                                                                           
GATGATATTTTAAAATTAATTAATGTCATCAGGTCCGAAAATAACGAGAATATTTCAGTCTCTCATCCTGTTGCGCTCCTGTCATGTGCATTGCTTCATATAATCACTGGCGCAAGGAGCGC‑GCAGGGGGCGG‑CCAATCGCCGCCGCCCCCTGCACCCCCGGGCTCTGGCGAACAAAATCGCCGCTGCGCGGTGCCCTCGGCTTATCCCTTACGGCTACCGGGTCGGGCGCGAGGTAACATCCCTGTAAAACGCGCCCTCAGCCCACATCCATGTGGG  >  NZ_CP025268/2233088‑2233365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑