Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,240,3030AT20.2% 591.9 / 33.8 251intergenic (+73/‑326)alx/sstTTerC family membrane protein Alx/serine/threonine transporter SstT
Reads supporting (aligned to +/- strand):  ref base A (137/57);  new base T (0/49);  total (137/114)
Fisher's exact test for biased strand distribution p-value = 6.52e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 268 total aligned reads displayed.

TCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NZ_CP025268/3240164‑3240442
                                                                                                                                            |                                                                                                                                           
tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgcga                                                                                                                                             >  2:11398797/1‑140 (MQ=255)
tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                             <  1:8228131/140‑1 (MQ=255)
tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                             >  2:6901830/1‑140 (MQ=255)
  tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           >  2:2397884/1‑140 (MQ=255)
    gagctgaCGTTTAGAATCAACGCCTGGGTGAAGTATCGGCCTGATAAGCAGCGGGGTGGATAAATTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                         <  2:9041749/136‑1 (MQ=255)
    gTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                         <  2:241819/140‑1 (MQ=255)
       atgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                      >  1:1013337/1‑140 (MQ=255)
       atgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                      >  1:10742270/1‑140 (MQ=255)
        tgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCggg                                                                                                                                     <  2:131569/140‑1 (MQ=255)
        tgaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCggg                                                                                                                                     >  1:3332457/1‑140 (MQ=255)
          aCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                                                                                   >  1:3844610/1‑140 (MQ=255)
            gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGgaga                                                                                                                                 >  1:1666500/1‑140 (MQ=255)
            gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                 >  1:1565609/1‑140 (MQ=255)
               tattatCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAggg                                                                                                                              >  2:8749651/1‑140 (MQ=255)
                      aaCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtgtt                                                                                                                       >  1:10864517/1‑140 (MQ=255)
                       aCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCTGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                      <  2:4932368/140‑1 (MQ=255)
                            ggggTTAATTATCGGCATGATAAGCTGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACACTGTCATTAACATGAGCCCCGTTTTCTCCCTCTCCCGTCCCGGGTGAGGGTCGGGGTGTGGTTAATTTTTCGCAc                                                                                                                 <  2:1377238/139‑1 (MQ=255)
                                 taaTTATCTGAATTATATGCTGCGGTGTGTATATTTTTTTATCTGCCTAA‑GCCGTGTACCTTGTAATTAACATGAGCACCTTTTTCTCCCTCTCCATTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACAGATg                                                                                                            <  2:7156881/139‑1 (MQ=255)
                                  aaTTATCGTCATTATAAGCAGCGGGGTGTTTATTTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAAATTGTGCACCGTTTTATCCCTCTCGCTTCCTGGTATTGGTTCGGGGTTTGGGTAATTTTTCGCACCGATGc                                                                                                           <  2:1642573/140‑1 (MQ=255)
                                      aTCGGCATGATAAGCCGCGGGGTGGATAATTTTTAATCTGCCTCA‑GCCGTGTACCCTGACATTAACATGAGCACCGTTTTCTCCCTCTCCCTGCCCGGGAGGGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGc                                                                                                       <  1:10378172/140‑1 (MQ=255)
                                      aTCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCATAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGTGGGTAATATTTCGCACCGATGCTGGc                                                                                                       <  2:5039237/140‑1 (MQ=255)
                                         ggCATTAAAAGCAGCGGGGTGGATAATTTTAAATCGGCCTAA‑GCCGTGTACCCTGTCATTAACATGCCCACCGTTTTCGCCCTCACCCTACCCGGGAGAGGGTCGGGGTGAGGGTCATTTTTCGCACCGATGCTGGCCTg                                                                                                    <  2:647844/140‑1 (MQ=255)
                                          gattGATATGCAGCGGGGTGGATATTTTTTAATCGGCATAA‑GCCGTGTCCCCTGTATTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGt                                                                                                   <  2:9339157/137‑1 (MQ=255)
                                          gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGt                                                                                                   >  2:5162857/1‑140 (MQ=255)
                                              gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTccc                                                                                               >  2:1448938/1‑140 (MQ=255)
                                               ttAAGCTGGGGGGTGTATAATTTTTAATCTGCCTAA‑GCGGTGTACCCTGTATTTAAAATGAGCACCGTTTTCTCCCTCTCCCTTCCTGGGTGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                              <  2:5448560/139‑1 (MQ=255)
                                               aTAAGCAGCGGGTTGTATATTTTTTAATCTGCATAA‑GCCGTTTACCTTGACATTAAAATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTGGGGGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                              <  2:8501399/140‑1 (MQ=255)
                                               aTAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCTTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTcccc                                                                                              >  1:8501399/1‑140 (MQ=255)
                                                tatgctgcGGGGTGGATAATTTTTAATCGGCCTAT‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  2:10482480/134‑1 (MQ=255)
                                                tatgcaggGGGGTGGATATTTTTTAATCTGCATAC‑GCGGTGTACCCTGTCATTAACAGGAGCACCTTTTCCTCCCTCCCCCTCCCTGGTTTTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGGTCCCCt                                                                                             <  2:3738168/137‑1 (MQ=255)
                                                tacgccgcGGGGTGGATATTTTTAAATCTGCCTAC‑GCCGTGTACCCTGTCATTAACTTGAGCACCTTTTTCACCCTCTCCCTTCCAGGGTGTGGGTCGGGGTGCGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  1:10630231/134‑1 (MQ=255)
                                                tAAGCCGGGGGGTGTATAATTTTTAATCTGCCTAC‑GCCGTGTACCCTTTAATTAACTTGAGCACCGTTTTCGCCCTCTCCCTTCCTGGGAGTGGGTGGGGGTGCGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  1:4831914/140‑1 (MQ=255)
                                                tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCTTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCTGGGAGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  2:4988177/140‑1 (MQ=255)
                                                tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  2:10896937/140‑1 (MQ=255)
                                                tAAGCAGCGGGGTGGATAATTTTAAATCTGCCTAA‑GCCGTGTACCCTGTCCTTAACATGCGCACCGATTTCTCCCACTCCCTTCCAGGGAGAGGGTCGGGGTCAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  2:9755480/140‑1 (MQ=255)
                                                aacgcaggGGGGTGTATAATTTTTAACCTCCATAC‑GCGGTGTACCCTTAAATTAACTTGAGCACCTTTTACCCCCTCCCCCTCCCCGGTAGTGGGTCGGGGTGTGGTAAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  1:5771889/137‑1 (MQ=255)
                                                 aaGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTc                                                                                            >  2:5536095/1‑140 (MQ=255)
                                                         gggTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAc                                                                                    >  1:348756/1‑140 (MQ=255)
                                                         gggTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAc                                                                                    >  2:7215559/1‑140 (MQ=255)
                                                          ggTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGAACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                   >  2:5797384/1‑140 (MQ=255)
                                                           gTGGATATTTTTTACTC‑GCCAAACGCCGTTAACCCTGACATAAAAATGAGACCTGTTTGCTCCCTCTCCCACCCCGGGAGTGGGGGGGGGTTTGGGCACTTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                  <  2:2221573/140‑1 (MQ=255)
                                                           gTGGATAATTTTTAATCTGCCTAC‑GCGGTGTACCCTGTAATTAACTTGCGCACCGTTTTCTCCCTCGCCCTTCCGGGGAGTGGGTGGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                  <  2:2846278/140‑1 (MQ=37)
                                                           gTGGATAATTTTTAATCTGCCTAA‑GCCGTTTACCCTGACATTAACATTAGCACCGTTTTCTCCCTCTCCCTTCCAGGTAGAGGGTCGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                  <  2:3844610/140‑1 (MQ=38)
                                                           gTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTATTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                  <  2:6511398/140‑1 (MQ=255)
                                                           gTGCATAATTTTTAATCTCCCAAA‑GCCTTGTACCCTGTCATTAACATGCGCACCTTTTTCCCCCCCTCCCTACCAGGTATAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAccc                                                                                  <  1:8781678/140‑1 (MQ=25)
                                                            tGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTGTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTCATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCct                                                                                 >  1:2713269/1‑140 (MQ=255)
                                                              gATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCctct                                                                               >  1:5017898/1‑140 (MQ=255)
                                                                      ttAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAc                                                                       >  2:4585191/1‑140 (MQ=255)
                                                                      ttAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAAc                                                                       >  2:5496979/1‑140 (MQ=255)
                                                                        attctcCCTAC‑GCGTTGTACCCTTTCATAAAAATGACCACTTTTTTCTCCCCCCCCATTCCGGGTTGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                     <  1:2834715/138‑1 (MQ=18)
                                                                        aaTCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                     >  1:2001598/1‑140 (MQ=255)
                                                                        aaTCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgg                                                                     >  1:11225854/1‑140 (MQ=255)
                                                                        aaTCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCGGGCCTGTTCCCCTCACCCTAACCCTCTCCCCCAACgg                                                                     >  1:3600894/1‑140 (MQ=255)
                                                                             gCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGa                                                                >  1:466393/1‑140 (MQ=255)
                                                                             gCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCACTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAGTTTTCGCTCCGATGCTGGCCTGTTCGCCTCTCCGTAACCCACTCCCCTAACGGGGCGa                                                                >  2:7405405/1‑140 (MQ=255)
                                                                              ccTAC‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCCCCCTCCCCGGGAGAGGGTCGGGGTGCGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               <  1:7668880/140‑1 (MQ=37)
                                                                              ccTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               >  1:4466838/1‑140 (MQ=255)
                                                                              ccTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               >  1:5250266/1‑140 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCTTTTTCTCCCTCTCCCTTCAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACAGATGCTGTCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  2:3969102/1‑140 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  2:9681582/100‑1 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  2:676217/1‑140 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  2:11165263/1‑140 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  2:5677199/1‑140 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  1:9244800/1‑140 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  1:4042804/1‑140 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  2:9069941/1‑140 (MQ=255)
                                                                                         gtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     >  2:9483549/1‑140 (MQ=255)
                                                                                         gtACCCTGTAATAAACATGCCCCCCGTTTACTCCCTCCCCCTACCTGGGTGTGGGTGGGGGTGTGTTAAAATTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGAcc                                                     <  1:141791/140‑1 (MQ=18)
                                                                                          tACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                    >  1:6438808/1‑140 (MQ=255)
                                                                                               tGTCATTAACAGGCGCCCCGTTTCCTCCCTCCCCCTCCCCGGGTGTGGGTCGGGGTGAGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTcc                                               <  1:6901830/140‑1 (MQ=25)
                                                                                                gTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTccg                                              >  1:8823376/1‑139 (MQ=255)
                                                                                                gTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCt                                              >  1:6094913/1‑140 (MQ=255)
                                                                                                  cATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttg                                            >  2:10200905/1‑139 (MQ=255)
                                                                                                      accATGCGCACCGTTTGCGCCCTCTCCCTGCCTGGGAGAGGTGCGGGGGGCGGGTAATTTTTCGCACCGCTGCTGGCCTGTTCCCCTCACCCTACCCCTCTCCCCAACCGGGGCGAGGGGACTGACCGAGTCCTTTTTTg                                        <  2:42071/138‑1 (MQ=21)
                                                                                                       aCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCAAACCCACTCCCCAAACGGGGCGAGGGGCCTGACCGAGTCCTTTTTTGa                                       >  1:8024392/1‑140 (MQ=255)
                                                                                                           gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             <  2:11607411/82‑1 (MQ=255)
                                                                                                           gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCt                                                                                             >  1:11607411/1‑82 (MQ=255)
                                                                                                           gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgtt                                   >  1:10332919/1‑140 (MQ=255)
                                                                                                           gAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTGTTTGAtgtg                                   >  1:3758833/1‑139 (MQ=255)
                                                                                                            aGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttg                                  >  1:10089870/1‑140 (MQ=255)
                                                                                                             gCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttgt                                 >  1:514243/1‑140 (MQ=255)
                                                                                                                ccGTTTTCTCCCTCTCCCTTCCAGGGTGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                              <  1:7798928/140‑1 (MQ=255)
                                                                                                                ccGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtcat                              <  2:5916813/140‑1 (MQ=255)
                                                                                                                    tttCTCCCTCTCCCTTCCCGGGAGTGGGTCGGGGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGt                          <  2:3558633/140‑1 (MQ=255)
                                                                                                                       ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGCGTCCTTTTTTGATGTTGTCATCAGTCTg                       >  1:1387068/1‑140 (MQ=255)
                                                                                                                        tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTGTTTTGATGTTGTCATCAGTCTgg                      >  2:7871717/1‑140 (MQ=255)
                                                                                                                         cccgccccctTCCCGGGAGTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGa                     <  1:6936230/134‑1 (MQ=37)
                                                                                                                           ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTGTTTTGTTGTTGTCATCAGTGTGGAAg                   >  1:8092665/1‑140 (MQ=255)
                                                                                                                            ccgccctGCCCGGGAGCGGGTGGGGGTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGc                  <  1:6204780/137‑1 (MQ=37)
                                                                                                                              tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGGCTGTTCCCCGCACCCGAACCCTCTCCCCAAACGGGGCGAGGGGACTGAGCGCGTCCTTTGTGGAGGTGGGCCTCCGTCTGGACGCCg                >  2:8319285/1‑140 (MQ=255)
                                                                                                                              tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCg                >  1:9795251/1‑140 (MQ=255)
                                                                                                                              tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCg                <  2:6115487/140‑1 (MQ=255)
                                                                                                                                cctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCCCCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCa              >  1:9568885/1‑140 (MQ=255)
                                                                                                                                  ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGCGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg            >  2:300006/1‑140 (MQ=255)
                                                                                                                                  ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg            <  2:10332928/140‑1 (MQ=255)
                                                                                                                                  ctCCAGGGCGAGGGTCCGGGTGAGGGTAATTTCACGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACCCCCCAAACGGGGCGACGTGACGGACCGAGTCCTTTTTTGATGTGGTCATCAGTCTGGAAGCCGCTCg            <  2:8265635/139‑1 (MQ=37)
                                                                                                                                        ggAGCGGGTCGGGGTGAGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt      <  1:9629970/140‑1 (MQ=255)
                                                                                                                                          atagGGTCGGGGTGAGGTTAATTTTTCGCACCTATGCTGGCCTGTTCCACTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAATGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAATCCGCACGTTGGCttt    >  2:4770652/3‑140 (MQ=37)
                                                                                                                                            agGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAt  >  2:1726841/1‑140 (MQ=255)
                                                                                                                                            agGGTCGGGGTGAGGGTAATTTGTCGCTCCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAATCGGGGCGAGGGGCGGGAGCGTGTCGTTTTTTGTTGTTGTCTTCTGTCTGGAAGCCGCACGTTGGCTTTAt  >  2:9466346/1‑140 (MQ=25)
                                                                                                                                            |                                                                                                                                           
TCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAA‑GCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NZ_CP025268/3240164‑3240442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑