Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0252683,505,5530TA21.3% 613.5 / 41.6 293intergenic (+84/+68)phoB/yhfSDNA‑binding transcriptional regulator FrlR/aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
Reads supporting (aligned to +/- strand):  ref base T (67/147);  new base A (57/1);  total (143/150)
Fisher's exact test for biased strand distribution p-value = 1.57e-22
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 296 total aligned reads displayed.

ACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCG  >  NZ_CP025268/3505415‑3505692
                                                                                                                                          |                                                                                                                                            
   cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                          <  1:4438832/140‑1 (MQ=255)
   cttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                          <  2:5529337/140‑1 (MQ=255)
    ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGGACGGTCCCCGCACCCTAAccc                                                                                                                                         >  2:6448465/1‑140 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCNTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  1:6163974/140‑1 (MQ=255)
     tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        <  2:9112462/140‑1 (MQ=255)
      ctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCctc                                                                                                                                       >  2:1872143/1‑140 (MQ=255)
      ctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAAcccac                                                                                                                                       >  1:10015011/1‑138 (MQ=255)
            cgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCGCACCCAAACCCGCACCCCa                                                                                                                                 >  2:667362/1‑140 (MQ=39)
                   cGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGGCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGc                                                                                                                          <  1:2682312/140‑1 (MQ=255)
                   cGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCAAAACCACTCCCCAAAGGGGc                                                                                                                          >  2:11584503/1‑140 (MQ=39)
                          ccTGAACTCTTGCAAATAACAGACAATAACCGTAGGCCGGATAAGATGCGCCAGCATCGCTTCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGa                                                                                                                   <  2:11300256/140‑1 (MQ=38)
                           cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACCCAACCCCCAAAGGGCGAgggggc                                                                                                                  >  1:4270663/1‑138 (MQ=37)
                           cTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCGAACCCTCTACCCAAACGGGCGCGGGGAc                                                                                                                  >  1:8699697/1‑140 (MQ=39)
                             tAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCTCTACCCAAAGGGGCGAGGGGACCg                                                                                                                >  1:9458176/1‑140 (MQ=255)
                               aCTATTGATAATAAAAGACATTAACCNTAGGCCGNATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACCTTCCCCTCACCCTAACCCTCTCCCNAAAGGGGCGAGGGGACCGtt                                                                                                              <  1:6102202/140‑1 (MQ=255)
                               aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTTCACGCACCCAAAACCACACCCCCAAAGGGGGTggggtgcggt                                                                                                              >  1:4119553/1‑134 (MQ=25)
                               aCTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGtt                                                                                                              <  1:7038165/140‑1 (MQ=255)
                                 tATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                            <  2:1555760/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑a                                                                                                        <  1:7216261/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑a                                                                                                        <  1:3228963/140‑1 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTACCCAAAGGGGCGAGGGGACCGTTCAGT‑a                                                                                                        >  2:6213863/1‑140 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCGAAACCTCACCCCAAAGGGGCGAGGGGACCGTTCAGT‑a                                                                                                        >  2:1673096/1‑140 (MQ=255)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCAAACCCAAACCCGCTACCCAAATGGGGCATGGGGCCGTGCAgga                                                                                                         >  1:220662/1‑138 (MQ=37)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACCCCCCAACCCACACCCCCAACGGGCGCGGGGACCGGTCAGT‑a                                                                                                        >  1:9250285/1‑140 (MQ=37)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACAACCCAAAAGGGCGAGGGGGCCCGTCAGT‑a                                                                                                        >  1:11000756/1‑140 (MQ=37)
                                    tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGATGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑a                                                                                                        <  1:10030394/140‑1 (MQ=255)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTAGATCCCGTACGTTCCCCGCACCCTAAGCCTCTCCCCCAAGGGGCGAGGGGACCGGTCAGT‑Ac                                                                                                       >  2:10111474/1‑140 (MQ=39)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCGCTCCCCAAAGGGGCGAGGGGACCGGTCAGT‑aa                                                                                                       >  2:2158486/1‑139 (MQ=255)
                                     gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACCCTAACCCTCACCCCAAACGGGCGAGGGGACCGTTCACT‑Ac                                                                                                       >  2:10487773/1‑140 (MQ=255)
                                        aataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAAACCACACCCCCAAAGGGCGCGGGGGCCCGTCAGT‑GCggg                                                                                                    >  1:7929896/1‑139 (MQ=25)
                                           aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAAtt                                                                                                 <  1:3880287/140‑1 (MQ=255)
                                           aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAAtt                                                                                                 <  1:6382031/140‑1 (MQ=255)
                                                  aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCTAACCCACTACCCAAAGGGGCGAGGGGTCCGGTCAGT‑AAGGCATTTTGCTTg                                                                                          >  1:5783533/1‑140 (MQ=37)
                                                  aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGT‑ACGGTATTTAGCTTg                                                                                          >  2:8607930/1‑140 (MQ=255)
                                                  aTTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTAAACCTAACCCACTCCCCCAAGGGGCGAGGGGACCCTTCAGT‑ACCGAAATTAGcatt                                                                                          >  2:443989/1‑137 (MQ=38)
                                                   ttcaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGa                                                                                         <  2:7967511/137‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGa                                                                                         <  2:4353199/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGa                                                                                         <  1:892908/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGa                                                                                         <  1:2568432/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGa                                                                                         <  2:504242/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGa                                                                                         <  2:4810179/140‑1 (MQ=255)
                                                   ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGa                                                                                         <  1:5953688/140‑1 (MQ=255)
                                                    tAACCGTAGGCCGGATAAGTTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCATT‑ACGGAATATAGCTTGAc                                                                                        >  2:9379981/1‑140 (MQ=255)
                                                    tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACAACCCAAAGGGGGGAGGGGACCGTTGAGT‑TAGGAAATTATCTTTAc                                                                                        >  1:10365832/1‑140 (MQ=37)
                                                    tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCACCACCAAACACCCACCCCCAAGCGGCGCGGGGTCCGTGAAGT‑ACCGCATTTAggatttg                                                                                        >  1:8053450/1‑134 (MQ=19)
                                                      aCCGTAGGCCGGAGCAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCACTCCCCCAAGGGGCGAGGGGACCGGTCAGT‑ACGGAATTTAGCTTGAccg                                                                                      >  2:4054790/1‑138 (MQ=39)
                                                        cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTg                                                                                    <  1:8008107/140‑1 (MQ=255)
                                                          tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGAAACCACACCCCAAAAGGGGGCGGGGGGCGGGCAGT‑ACGGCAATTAGCTGGAAgggggt                                                                                  >  1:95215/1‑136 (MQ=19)
                                                           aGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCCACCCCAAAACCCCAACCCCAAGTGGGGGGGGGGCCGGTGAGTAAGGGAATTTGGATTGaggtggga                                                                                  >  1:9831348/1‑133 (MQ=16)
                                                            ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCct                                                                                                                                        >  2:9601552/1‑85 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATc                                                                               <  1:2314946/140‑1 (MQ=255)
                                                             gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATc                                                                               <  1:2031144/140‑1 (MQ=255)
                                                               cGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCa                                                                             <  1:10665012/140‑1 (MQ=255)
                                                               cGGATAAGATGCGCCACCATCGCATCCGGCGATGCTGGCGCGTAGAATTTTACAGCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCCCAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCa                                                                             <  2:10867071/140‑1 (MQ=255)
                                                                 gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGAACCCACAACCCAAAGGGGCGGGGGGGCCGGTAAGT‑ACGGAAATTAGCTTTTCGGTGCTATACacc                                                                           >  1:539999/1‑138 (MQ=255)
                                                                 gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTCACCCTCTCCCCAAAGGGGCGAGGGGAACCTTCAGT‑ACGGAAATTAGCTTGACGGTGCTATCCAGc                                                                           >  2:2510817/1‑140 (MQ=39)
                                                                 gATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGAGGGGCACGTTCAGT‑AAGGAATTTAGCTTGACCGTGCTATACAGc                                                                           >  2:1417252/1‑140 (MQ=255)
                                                                  aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTAGGTTACCCACACCCAATCCCTCTACCCAAAGGGGCGAGGGGACCGTTCAGT‑TCGGAATTTATGTTTTCGGTGCTATCCAGCa                                                                          >  2:8440538/1‑140 (MQ=255)
                                                                  aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTCACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                          <  2:2554864/140‑1 (MQ=255)
                                                                  aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCCCACCCCCAAGGGGCGAGGGGCCCGTTCAGT‑ACGGAATTTAGCGTGACGGTGCTATCCAGCa                                                                          >  1:7826246/1‑140 (MQ=255)
                                                                       aTGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCACACCCCAAAGGGGCGCGGGGACCCGTCAGT‑ACGGAAATTAGCTTGACGGTGCTATACAGCATTaaa                                                                     >  2:4210155/1‑140 (MQ=255)
                                                                        tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTC‑CCCTAACCCCCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATa                                                                   >  2:5816684/1‑140 (MQ=255)
                                                                          cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTACCCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                  <  1:11312216/140‑1 (MQ=255)
                                                                          cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                  <  1:2998442/140‑1 (MQ=255)
                                                                          cgcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAc                                                                  <  1:1161548/140‑1 (MQ=255)
                                                                           gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTGCCCCCCCCCAAAACCACAACCCACAGGGGCGCGGGGTCCGTGAAGG‑ACGGAATTTATCTTTACGGTGCTATCCAGCACTaaaaaaa                                                                 >  1:8454416/1‑136 (MQ=255)
                                                                           gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACt                                                                 <  1:11089709/140‑1 (MQ=255)
                                                                            ccAGCATCGCATCCGGCGATGCTGGCGCGTTGAATGTTACAGCCCGTACGTTCCCCTAACCCTAACCCTCTCCCCAAACGGGCGAGGGGACCGATCAGT‑ACGGAAGTTAGCTTGACGGTGCTATCCAGCCTTAAATACTg                                                                >  2:10984596/1‑140 (MQ=38)
                                                                                catcgcatcCGGCGATGCGGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCAAAACCACTCCCCCAAAGGGCGTGGGGCCCGTTCAGT‑AAGGAAATTATCTTGACGGGGCGATCCAGCATTAAAAACAGGCaa                                                            >  2:3997936/1‑140 (MQ=255)
                                                                                     catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTATACCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCATGACGGTGCTATCCAGCATTAAAAAATGGCAATActc                                                       >  2:722836/1‑140 (MQ=39)
                                                                                     catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGGTTTACGGTGCTATACAGCATTAAATACTGGCAAAActc                                                       >  2:527700/1‑140 (MQ=38)
                                                                                     catcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACCCCCAAACCCACACCCCAAAGGGGCGAGGGGACCGGTAAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATAAAGGCAAAAcac                                                       >  2:4577416/1‑138 (MQ=255)
                                                                                         cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCCAAACCACTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑AACGAAATTATCTTTTACGTGCTATACAGCAATAAATAAATGCAATaatataac                                                   >  2:3316554/1‑133 (MQ=255)
                                                                                         cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                   <  2:3056727/140‑1 (MQ=255)
                                                                                              aTGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                              <  2:9889053/140‑1 (MQ=255)
                                                                                              aTGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                              <  2:5890210/140‑1 (MQ=255)
                                                                                              aTGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                              <  1:11516550/140‑1 (MQ=255)
                                                                                                gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAAt                                            <  1:6881697/140‑1 (MQ=255)
                                                                                                   ggCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                         <  1:2039524/140‑1 (MQ=255)
                                                                                                     cgcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCCTTAAATACTGGCAATACTCTCACGCCAAATACgca                                       >  2:7395293/1‑140 (MQ=255)
                                                                                                     cgcgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgca                                       <  2:9250774/140‑1 (MQ=255)
                                                                                                       cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgcagc                                     <  2:872544/140‑1 (MQ=255)
                                                                                                         ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                   <  2:11039796/140‑1 (MQ=255)
                                                                                                         ttGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                   <  2:3385277/140‑1 (MQ=255)
                                                                                                         ttGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAc                                   <  1:4659553/140‑1 (MQ=255)
                                                                                                          tGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                  <  2:7193182/140‑1 (MQ=255)
                                                                                                            aaTTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTACAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                                <  2:8021052/140‑1 (MQ=255)
                                                                                                                 tACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                           <  1:2885243/140‑1 (MQ=255)
                                                                                                                    aTCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                        <  2:4944767/140‑1 (MQ=255)
                                                                                                                       ccGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGTACAGT‑ACGGAATTTAGCTTGACGGTGCGATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGc                     <  2:6280935/140‑1 (MQ=255)
                                                                                                                        cGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCt                    <  1:1673096/140‑1 (MQ=255)
                                                                                                                         gTACGTTCCCCGCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGT‑ACGGAATTTAGCTTGACGGTGCTATACAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTg                   >  2:2933125/1‑140 (MQ=255)
                                                                                                                             gTTCCCCTCACCCTAACCCTCTCCCCCAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt               <  1:8233080/140‑1 (MQ=255)
                                                                                                                             gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt               <  1:1762099/140‑1 (MQ=255)
                                                                                                                             gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTGAGCTTGACGGTGCGATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAt               <  2:7074910/140‑1 (MQ=255)
                                                                                                                              ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGAtt              <  2:2692455/140‑1 (MQ=255)
                                                                                                                               tCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTc             <  1:3002606/140‑1 (MQ=255)
                                                                                                                                acccACACCCAAAACCTCAACCCAAACGGGAGATGGGTCCCTTCATT‑ACCGAAATTATCTTTACGGTGCTATACCCCCTTAAATAATGGCAAAACTATAAAGCAAAATCCGCAGCACCGTCTAATAACCGCAGCGAttag            >  2:3664435/2‑138 (MQ=255)
                                                                                                                                  ccTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg          <  1:1476132/140‑1 (MQ=255)
                                                                                                                                  ccTCACCCGAACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGA‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg          <  2:11045503/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                            
ACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGT‑ACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATGCG  >  NZ_CP025268/3505415‑3505692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑