Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,3950AT21.0% 342.4 / 29.9 191intergenic (+30/‑166)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (70/77);  new base T (0/39);  total (70/121)
Fisher's exact test for biased strand distribution p-value = 7.27e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

GAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTT  >  NZ_CP025268/164256‑164533
                                                                                                                                             |                                                                                                                                           
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGg‑aga                                                                                                                                             >  1:4181803/1‑140 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGg‑aga                                                                                                                                             <  1:7117819/140‑1 (MQ=255)
gAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGg‑aga                                                                                                                                             >  2:4904107/1‑140 (MQ=255)
  gCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAgg                                                                                                                                           >  1:8237887/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCTTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCGCCCTGTGGG‑AGAggg                                                                                                                                          <  2:8947032/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAggg                                                                                                                                          <  1:10132471/140‑1 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAggg                                                                                                                                          >  1:8810604/1‑140 (MQ=255)
   cGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAggg                                                                                                                                          >  2:11283758/1‑140 (MQ=255)
    gTACCGTGAGGTGCAAAAAGAGATGACAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGc                                                                                                                                         <  1:3730736/140‑1 (MQ=255)
    gTACCGTGAGGAGCAACACTAGAAGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGTCAAATACTGCACCGACGCGACTGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGc                                                                                                                                         <  2:6927302/140‑1 (MQ=255)
     tACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGCCTGGCCGGCCGACCCGGAAAATACTGCACCGCCGCGACGGACTAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGG‑AGAGGGcc                                                                                                                                        <  1:10026233/140‑1 (MQ=255)
      aCCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCg                                                                                                                                       <  2:9301542/140‑1 (MQ=255)
        cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCggg                                                                                                                                     <  1:6664696/140‑1 (MQ=255)
        cGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCggg                                                                                                                                     <  2:10302952/140‑1 (MQ=255)
               tGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAggg                                                                                                                              >  1:3203218/1‑140 (MQ=255)
                gCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGc                                                                                                                             >  1:1332715/1‑140 (MQ=255)
                 cAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCa                                                                                                                            >  1:9050757/1‑140 (MQ=255)
                 cAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCa                                                                                                                            >  2:8053065/1‑140 (MQ=255)
                 cAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCa                                                                                                                            >  1:8337918/1‑140 (MQ=255)
                  acaaaGATATGAAAGGGCGTTATCCCAAACA‑TGTATGGCCGGACGACCCGGAAAATACGGCACCGACGCGACGGAGGAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCGCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCAt                                                                                                                           <  2:9800041/138‑1 (MQ=38)
                  aaaaaGAGATGAAAGGCCGTTATCCCAAACA‑TGACTGGCCGGACGACCCGGCAAATACTGCACCGCCGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCACCCTGTGGG‑AGAGGGCCGGGGTGAGGGCAt                                                                                                                           <  2:10654669/140‑1 (MQ=39)
                       cagtttaaaGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGAAAATACTGCCCCGACGCGACGGACGAAAATTTATTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGG‑TGAGGGCCGGGGTGAGGGCATCAgcg                                                                                                                      <  2:11600255/134‑1 (MQ=38)
                        agaTTAAAGGGCTTTATCCCAAACA‑TGTCTGGCCGTACGACCCGCCAAATACTGCACCGACGCGACGGACTAAAAATTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGTGGGCATCAgcgc                                                                                                                     <  2:8276514/140‑1 (MQ=37)
                         gaTGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTCTTCGGAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGGGAGGGCGTCcgcgcg                                                                                                                    >  1:8993367/1‑140 (MQ=39)
                         gaTGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAgcgcg                                                                                                                    >  2:510795/1‑140 (MQ=255)
                           tGAAAGGGCGTTATCCCACACA‑TGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGT‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                  <  2:8677417/140‑1 (MQ=255)
                           tGAAAGGGCGTTAACCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCa                                                                                                                  >  1:9991502/1‑140 (MQ=255)
                              aaGGGCGTTATCACAAACA‑TGTCTGGCGGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAATTTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGGGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGt                                                                                                               <  2:1376818/140‑1 (MQ=37)
                               tgggCGTTATCCCAAACT‑TGTCGGGCCGGACGACCCGGCAAATCCTGCACCGACGCGCCGGACTAAAAAGTATTCGTACGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGTGGGCATCAGCGCGCACGtt                                                                                                              <  2:9339960/139‑1 (MQ=37)
                               cgggCGTTAACCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCTTATGGTTGTTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGtt                                                                                                              <  2:6060834/139‑1 (MQ=38)
                               aGGGCGTTCTCCCCACCA‑TGTCTGGCCGGCCGACCCGGCAAAGACTGCACCGACGCGACGGACGAAACCGAATTCGTATGGTTGGTTTTCTCCCGCTCCCTGTGGG‑AGAGGGCCGGGGGGAGGGCATCAGCGCGCACGtt                                                                                                              <  2:6455485/140‑1 (MQ=25)
                               aGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGtg                                                                                                              >  1:5544432/1‑139 (MQ=255)
                                   ggTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTtcacc                                                                                                          <  2:296871/139‑1 (MQ=255)
                                   cGTTATCCCAAACA‑TGTCGGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGTTTTTCTCCCTCACCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacc                                                                                                          <  2:1995969/140‑1 (MQ=255)
                                   cGTCATCCCAAACA‑TGTCTGGCCGGACGACCCGCCAAATACTGCACCGACGCTACGGACGAAAAAGTATACGTAAGGTTGGTTTTCTCCATCTCCATGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcacc                                                                                                          <  2:6496176/140‑1 (MQ=38)
                                    gTTATCCAAAACA‑TGTCTGGCCGGACCCCCCGGCAAATCCTGCACCGCCCCGACGGACGAAAAAGTAGTCGTAAGGTTGTGTTTCTCCCACTCCCTGTGGG‑AGAGGCCCGGGGGGCGGGCATCAGCGCGCACGTtcaccc                                                                                                         <  2:10870566/140‑1 (MQ=255)
                                     ttATCCCACACA‑TGTCTGGCCGGACGCCCCGGCAAAGACTGCACCGACGCGCCGGACGAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                        <  2:10561028/140‑1 (MQ=37)
                                     ttATCCCAAACT‑TGTCTGGCCGGACGACCGGGAAAATACGGCACGGACGCTACGTACTAAAAAGTATTCGTATGTTTGTTTTTCTCCCTCTCAATGTGGT‑TTAGGGCGGGGTTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  2:7130950/140‑1 (MQ=255)
                                     ttATCCCAAACA‑TTTCTGGCCGGACGACCCGTAAAATCCTGCACCTACGCGACGGACTAAAAAGTTTTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  2:5805553/140‑1 (MQ=37)
                                     ttATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  2:8739504/140‑1 (MQ=255)
                                     ttATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                        <  2:9529313/140‑1 (MQ=255)
                                     ttATCCCAAACA‑TGTCTGGCCGGACGACCCGGAAAATCCGGCACCGACGCGACGGACGAAAATGTATTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGT‑TGAGGGCGGGGGTGTGGGCCTCAGCGCGCACGTtcaccct                                                                                                        <  2:3551524/140‑1 (MQ=37)
                                     ttATCCCAAAAA‑TGAGTGGCCGCACGCCCCGCAAAAACCGGCACCGACGCGACGTACAAAAAAGTATTCTTAAGGTTGTTTTTCACCCTCCCCCTGTGGT‑TGTGGGCGGGGTTGCGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  2:325693/140‑1 (MQ=255)
                                     ttATCCAAAACA‑TGTGTGGCCGTACGACCCGTAAAATACTGAACCGACGCGCCGGACTAAAAAGTATTCGTAAGTTTGTTTTTAACCATCTCCCTGTGGG‑TGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  2:30171/140‑1 (MQ=25)
                                     cgagcccAATCC‑TGTCTGCCCGGCCGACCCGGACAATCCTGCACCGACGCGACGCCCGATAACGTATTCGACTGGTGGGTTGTCTCCCTCTCCCTCTGGG‑AGAGGGCCGGGTTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                        <  2:9448417/136‑1 (MQ=16)
                                      taacccAAACATTGTTTGGCCGGACGCCCCGTAAAATACTGCACCGACGCGCCGTACTAAAATGTTTACTTAAGTTTGTTTTTCTCCCACTCCCTGTGGT‑AGTGGGCCGGGGTGAGGCCATCAGCGCGCACGTtcaccct                                                                                                        <  2:3442456/137‑1 (MQ=255)
                                       aTCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccctca                                                                                                      <  2:10346489/140‑1 (MQ=255)
                                           cAAACA‑TGTCTGGCGGGCCGCCCCGGCAAATACTCCACCGCCGCGAGGGACTAAAATGTTTTCGAACGGTTGGTTTTCACCCACGCCGTGGGGG‑AGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTcaccctcaccct                                                                                                  <  2:4320594/140‑1 (MQ=255)
                                           cAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTCTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTgggcccccacccc                                                                                                  >  1:5847238/1‑139 (MQ=37)
                                             aaCA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGGACCCGCCACCTaa                                                                                                >  1:4867577/1‑140 (MQ=38)
                                             aaCA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtgacccccacccaca                                                                                                >  1:8875112/1‑137 (MQ=255)
                                              aCA‑TGTCTGGCCGGCCTACCCGGCAAATCCTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAc                                                                                               <  2:2820280/140‑1 (MQ=38)
                                                a‑TGTCTGGCCGTACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                             <  2:1534945/140‑1 (MQ=255)
                                                   gacTGGCCGTACCCCCCGCCAAATACTGCACCTACGCTACGTACTAAAAATTATTCTAACGGTTGTTTTTCTCCCTCCCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                           <  1:2880229/138‑1 (MQ=21)
                                                   gTCTGGCGGGACGACCCGTCAAATACTGCACCGACGCGACGGACGAAAATTTATTCTTATGGTTGTTTTACTCCCTCACCCTGTGGG‑CGTGGGCCGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                           <  1:9557765/140‑1 (MQ=255)
                                                   gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTTCACCCACACCCAAAcccac                                                                                           >  2:5581195/1‑138 (MQ=255)
                                                   gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAAcccac                                                                                           >  2:9247136/1‑138 (MQ=255)
                                                   gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCGCCCGCACCCGAAcccac                                                                                           >  1:7423684/1‑138 (MQ=38)
                                                   gTCTCGCCGGCCGACCCGGCAAATACTGCACCGACGCGACGTCCGAAAAATTATTCTAATGGTTGGTTTTCTCCCTCTCCCTGGGGG‑AGAGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                           <  1:10536375/140‑1 (MQ=37)
                                                   gTCGGGCCGGACGCCCCGCCACATACTGCACCTACGCGACGGCCGAAAAATTAGTCGAATGTTTGGTTTGCCCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                           <  1:7688529/140‑1 (MQ=25)
                                                    tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCTACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCCCCCTCACCCTAACCctct                                                                                          >  2:7795419/1‑140 (MQ=255)
                                                    tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCTCCCACACCCGAAACcact                                                                                          >  1:3918838/1‑140 (MQ=37)
                                                     cgggCGGGACGACCGGGAAAATACGGCACCCCCGCGCCGGACTAAAAATTATTCTTATGGTGGTTTTTCTCCCACTCCCTGTGGT‑AGTGGGCCGGGTTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                         <  1:2973873/138‑1 (MQ=255)
                                                     cgggCCGGACGACCCGCCACCTCCTGCCCCGACGCGCCGGACGAAAATTTATTCGTAAGGTGGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                         <  1:1840354/138‑1 (MQ=25)
                                                     cTGGCCTTACGACCCTGCAAATACTGCACCGACGCGACGGACTAAAAATTATTCTTATGGTTGGTTTTCTCCCTCTCCCTGTGTG‑TTTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                         <  2:2523927/140‑1 (MQ=37)
                                                     cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTTCACCCACACCCTAACCctcac                                                                                         >  2:3732201/1‑138 (MQ=255)
                                                     cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCAAACCCtctc                                                                                         >  2:11034152/1‑140 (MQ=255)
                                                        gCCGGACGCCCCGGCAAAAACGCCACCGACGCGCCGGACTAAACAGTATGCGTACGGGGGTTTGTCTCCCACGCCCGGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCt                                                                                      <  2:10794729/140‑1 (MQ=21)
                                                        gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGGGCCCGCACCCAAACCCACAccca                                                                                      >  1:8916082/1‑139 (MQ=37)
                                                                 cccgcaaaaTCCTCCACCGCCGCGCCGGACCAAAATTTATTCGTCTGGTTGTTTTCCCCCCGCCCCGTGTGGG‑TGGGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga                                                                             <  1:10794839/134‑1 (MQ=255)
                                                                  ccGGCAAATACTGCACCGACGCGACGGACGAAAAATTATTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                            <  2:1591420/140‑1 (MQ=255)
                                                                  ccGGCAAATACTCCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                            <  2:9066907/140‑1 (MQ=255)
                                                                       aaaTCCTGCACCGCCGCGCCGGACCAAAATGTATGCTTAAGGTTGGTTTCCCCCCACTCCCTGTGGC‑CGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                       <  1:9402301/140‑1 (MQ=255)
                                                                               cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCGGTCACCCACACCCGAAACCACACCCACAAGGGGGTGGGGGCCGATCGAg                                                               >  2:5225062/1‑140 (MQ=25)
                                                                               cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCAAAACCAAAACCACAAGGGGGAGGGGGCCGATagag                                                               >  2:1233230/1‑140 (MQ=21)
                                                                                    acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCACACCCAAAACCACGCCCACAAGGGAGAGGGGACCGATACAG‑CACaa                                                         >  1:4011316/1‑140 (MQ=25)
                                                                                     cgcgacgTAAGAAAATTTTTTCTTAGGGTTGTTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACaaa                                                        <  1:1530045/140‑1 (MQ=37)
                                                                                         acggactAAAAAGTATTCGTATGTTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAAtttt                                                    <  2:8916082/140‑1 (MQ=255)
                                                                                         acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGGTCACCCGCACCCGAACCCACTCCCGGGAGGGGGGGGGGGGCGGTGGGG‑CACAAAtttg                                                    >  1:10590127/1‑139 (MQ=21)
                                                                                           ggacgAAAATGTATTGGTAAGGTGGGTTTTCGCCCTCTCCCTGGGGT‑TGCGGGCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTga                                                  <  2:4087631/140‑1 (MQ=37)
                                                                                            gactAAAATTTATTCTTATGTTTGTTTTTCTCCATCGCCCTGTGGG‑TGTGGGCCGGGGTCTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgag                                                 <  1:8422513/140‑1 (MQ=37)
                                                                                              cgAAAATGTATTCGTAAGGTTGTTTTTCTCCATATCCGTGTGGG‑AGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                               <  2:2425812/140‑1 (MQ=39)
                                                                                              cgAAAAATTATTCGTATGGTGGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                               <  1:2958407/140‑1 (MQ=255)
                                                                                                  aaaGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGatat                                           <  2:1845747/140‑1 (MQ=255)
                                                                                                   aatttttCGTCCGGTTGGTTTTCCCCCTCCCCCTGTGGG‑TGTGGCCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtc                                          <  1:1171527/135‑1 (MQ=37)
                                                                                                   aaGTATTCGTAAGTTGGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGCCCGGGGTGAGTGCATCAGCGCGCACGTTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGTACCGATCGAG‑CACAAATTGTGAGAGATAtc                                          <  2:6677123/140‑1 (MQ=38)
                                                                                                   aaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtc                                          <  2:3993761/140‑1 (MQ=255)
                                                                                                     gttttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGACTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtctt                                        <  1:7576052/137‑1 (MQ=255)
                                                                                                      tATTCTTATGGTTGTTTTTCTCCCTCTCCCTGGGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttc                                       <  1:10344839/140‑1 (MQ=255)
                                                                                                        ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCCAAAACACACCCACCAGGGAGCGGGGACCCAACGAG‑CACAAAAATTGAGAGataaataaat                                     >  1:4033458/1‑133 (MQ=21)
                                                                                                         tCGTATGGGTGTTTTTCCCCCACTCCC‑GTGGGTTGTGGGCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                    <  1:6078579/140‑1 (MQ=25)
                                                                                                         gcttttggttggttTTCTCCCTCTCCCTGGGGT‑TGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                    <  1:6472678/134‑1 (MQ=38)
                                                                                                              tggtgggttTTCCCCCTCACCGTGGGGG‑AGGGGGGGGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCt                               <  1:1233230/139‑1 (MQ=37)
                                                                                                                 ctggtcTCCTCCCGCTCCCTGTGGG‑CGAGGGCCGGGGTGCGGGCCTCACCGCGCACGTACACCCTCCCCCTACCCCTCTCCCTCAAGGGAGCGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                            <  2:9666206/139‑1 (MQ=255)
                                                                                                                      tttCTCCCTCTCCGGGGGGG‑AGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                       <  1:7912655/140‑1 (MQ=39)
                                                                                                                      tttCTCCCTCTCCCTGTAGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                       <  1:2656325/140‑1 (MQ=255)
                                                                                                                      tatctccctctcccTGTGGG‑AGTGGGCCGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                       <  1:11011267/138‑1 (MQ=255)
                                                                                                                        tcccccgctccctGTGTG‑AGCGGGCCGGGGTTAGGTCCTCAGCGCGCACGTTCACACTCACACTAACCCTCTCCCTAAAGGGAGAGGGGACCGATCGCG‑CACAAATTTTGATAGATATCTTCTTCTGTAGTGTAACagaa                     <  1:682513/137‑1 (MQ=255)
                                                                                                                                      tgGG‑AGAGGGCCGGGGTGAGGGCATCAGCGGGCACGTTGACCCGAAACCCAAACCAAAACCACCATGGAGTGGGGTCCCATCGAGCCACAAATTTT‑TGAGTTATATTATTATTTGTTGTAAAAGAATAAAAGAAAATAgg       >  2:3175787/1‑140 (MQ=255)
                                                                                                                                           agagGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCACACCCAAAACCACTCCCACCAGGGCGAGGGGACCCATCGAG‑CACAAATTTTGAGAGATATCATATTATGTATTTTAAAAGTAGAAAAGAAAAAAGGGcgt   >  2:10918227/1‑138 (MQ=255)
                                                                                                                                             |                                                                                                                                           
GAGCGTACCGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTT  >  NZ_CP025268/164256‑164533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑