Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,4320TA23.8% 312.4 / 11.8 135intergenic (+67/‑129)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (4/89);  new base A (29/0);  total (46/89)
Fisher's exact test for biased strand distribution p-value = 4.24e-24
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 160 total aligned reads displayed.

TTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGA  >  NZ_CP025268/164293‑164570
                                                                                                                                             |                                                                                                                                           
ttATCCCACACA‑TGTCTGGCCGGACGCCCCGGCAAAGACTGCACCGACGCGCCGGACGAAAAAGTTTTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTtcaccct                                                                                                                                             <  2:10561028/140‑1 (MQ=37)
ttATCCCAAACT‑TGTCTGGCCGGACGACCGGGAAAATACGGCACGGACGCTACGTACTAAAAAGTATTCGTATGTTTGTTTTTCTCCCTCTCAATGTGGT‑TTAGGGCGGGGTTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                                             <  2:7130950/140‑1 (MQ=255)
ttATCCCAAACA‑TTTCTGGCCGGACGACCCGTAAAATCCTGCACCTACGCGACGGACTAAAAAGTTTTCTTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                                             <  2:5805553/140‑1 (MQ=37)
ttATCCCAAACA‑TGTCTGGCCGGACGCCCCGGCAAATACTGCACCGACGCGACGGACGAAAATTTATTCGTACGGTTGGTTTTCGCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                                             <  2:1652171/140‑1 (MQ=38)
ttATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                                             <  2:8739504/140‑1 (MQ=255)
ttATCCCAAACA‑TGTCTGGCCGGACGACCCGGAAAATCCGGCACCGACGCGACGGACGAAAATGTATTCGTATGTTTGTTTTTCTCCCTCTCCCTGTGGT‑TGAGGGCGGGGGTGTGGGCCTCAGCGCGCACGTtcaccct                                                                                                                                             <  2:3551524/140‑1 (MQ=37)
cgagcccAATCC‑TGTCTGCCCGGCCGACCCGGACAATCCTGCACCGACGCGACGCCCGATAACGTATTCGACTGGTGGGTTGTCTCCCTCTCCCTCTGGG‑AGAGGGCCGGGTTGAGGGCATCAGCGCGCACGTtcaccct                                                                                                                                             <  2:9448417/136‑1 (MQ=16)
 taacccAAACATTGTTTGGCCGGACGCCCCGTAAAATACTGCACCGACGCGCCGTACTAAAATGTTTACTTAAGTTTGTTTTTCTCCCACTCCCTGTGGT‑AGTGGGCCGGGGTGAGGCCATCAGCGCGCACGTtcaccct                                                                                                                                             <  2:3442456/137‑1 (MQ=255)
     ccAAAAA‑TTTCTGGCCGTACGACCGGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCGGGGGTTAGGGCATCAGCGCGCACGTtcaccctcaccc                                                                                                                                        <  2:1544560/140‑1 (MQ=38)
      cAAACA‑TGTCTGGCGGGCCGCCCCGGCAAATACTCCACCGCCGCGAGGGACTAAAATGTTTTCGAACGGTTGGTTTTCACCCACGCCGTGGGGG‑AGAGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTcaccctcaccct                                                                                                                                       <  2:4320594/140‑1 (MQ=255)
      cAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTCTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTgggcccccacccc                                                                                                                                       >  1:5847238/1‑139 (MQ=37)
      cAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTcaccctcaccct                                                                                                                                       >  2:10794839/1‑140 (MQ=255)
        aaCA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtgacccccacccaca                                                                                                                                     >  1:8875112/1‑137 (MQ=255)
         aaattGTCGGGCCGTACTACCCGGAAAATACTGAACCTCCGCTTCGTACTAAAATGTTTTCGTAGGTTTGTTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTaa                                                                                                                                     <  1:7534278/136‑1 (MQ=16)
         aCA‑TGTCTGGCCGGCCTACCCGGCAAATCCTGCACCGACGCGACGGACGAAAAAGTTTTCGTAAGGTTGGTTTTCTCCCTCTCCCGGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAc                                                                                                                                    <  2:2820280/140‑1 (MQ=38)
          cA‑TGTGTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAcc                                                                                                                                   <  2:4038173/140‑1 (MQ=255)
           a‑TGTCTGGCCGTACGACCCGGAAAATACTGCACCGACGCGACGGACGAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                                                  <  2:1534945/140‑1 (MQ=255)
            ttGTCGGGCGGGACGACCCGGAAACCAGTGCCCGGACGCGAGGTCCGAAAATGTTTGCTTACGGTTGGTTTACTCCCTCTCCCTGTGGG‑AGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAAccc                                                                                                                                  <  1:3031067/139‑1 (MQ=255)
              gacTGGCCGTACCCCCCGCCAAATACTGCACCTACGCTACGTACTAAAAATTATTCTAACGGTTGTTTTTCTCCCTCCCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                <  1:2880229/138‑1 (MQ=21)
              gTCTGGCGGGACGACCCGTCAAATACTGCACCGACGCGACGGACGAAAATTTATTCTTATGGTTGTTTTACTCCCTCACCCTGTGGG‑CGTGGGCCGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                <  1:9557765/140‑1 (MQ=255)
              gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                <  2:10706072/140‑1 (MQ=255)
              gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCTAAcccac                                                                                                                                >  2:9247136/1‑138 (MQ=255)
              gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCGAAaccac                                                                                                                                >  1:10490356/1‑138 (MQ=255)
              gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCGCACCCCaaaacaa                                                                                                                                >  1:6550746/1‑135 (MQ=37)
              gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAAcccaa                                                                                                                                >  1:10114715/1‑138 (MQ=255)
              gTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCGCCCGCACCCGAAcccac                                                                                                                                >  1:7423684/1‑138 (MQ=38)
              gTCTCGCCGGCCGACCCGGCAAATACTGCACCGACGCGACGTCCGAAAAATTATTCTAATGGTTGGTTTTCTCCCTCTCCCTGGGGG‑AGAGGGCCGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                <  1:10536375/140‑1 (MQ=37)
              gTCGGGCCGGACGCCCCGCCACATACTGCACCTACGCGACGGCCGAAAAATTAGTCGAATGTTTGGTTTGCCCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                <  1:7688529/140‑1 (MQ=25)
               tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCcact                                                                                                                               >  2:10344839/1‑140 (MQ=255)
               tCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCTCCCACACCCGAAACcact                                                                                                                               >  1:3918838/1‑140 (MQ=37)
               tCTGCCCGGACGCCCCGCCAAATACTGCACCGACGCGACGCACGAAAAAGTATTCGTAAGGTTGGTTTGCTCCCTCGCCCTGTGGG‑AGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCctct                                                                                                                               <  2:6379155/140‑1 (MQ=255)
                cgggCCGGACGACCCGCCACCTCCTGCCCCGACGCGCCGGACGAAAATTTATTCGTAAGGTGGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGCGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                                              <  1:1840354/138‑1 (MQ=25)
                cTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTTCACCCACACCCCAACCCactc                                                                                                                              >  2:3732790/1‑140 (MQ=255)
                 tGGCCGGAGGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCACTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                                                             <  2:9591777/140‑1 (MQ=255)
                   gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCGTGCACCCGCACCCGAACCCACAccca                                                                                                                           >  2:2762805/1‑139 (MQ=37)
                       gccgacCCTGAAAATCCTCCACCGCGGCGAGGTACTAAAATTTATTCTTTTGGTTGGTTTTCTCCCTCTCCCGGTGGT‑AGTGGGCGGGGGTGTGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                                       <  1:5544691/138‑1 (MQ=255)
                       gacgccCCGGCAAATACGGCACCGCCGCGCCGGACGAAAAAGTATTCGAACGGTTGGTTTTCTCCCTCCCCCTGTGGG‑AGAGGGCCGGGGGGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                                       <  2:3021099/140‑1 (MQ=38)
                       gaccacCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATCCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                                       <  1:7592115/140‑1 (MQ=255)
                           ccccGGCAAACACCCCCCCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCCCCCGCGCCCGGGGGG‑CGCGGGCCGGGGTGCGGGCAGCAGCGCGCACGTTCACCCTCACCCTCACCCTCTCCCTCAAGGgag                                                                                                                   <  2:8923856/139‑1 (MQ=255)
                           acCCGCAAAATACTGCCCCGACGCGACGGACTAAAATTTATTCGTATGGTGGGTTTTCTCCCTCGCCCTGTGGG‑TGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                   <  1:10312989/140‑1 (MQ=255)
                            cccgcaaaaTCCTCCACCGCCGCGCCGGACCAAAATTTATTCGTCTGGTTGTTTTCCCCCCGCCCCGTGTGGG‑TGGGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgaga                                                                                                                  <  1:10794839/134‑1 (MQ=255)
                             ccGGCAAATACTGGACCGACGCGACGGACTAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                 <  2:1852130/140‑1 (MQ=255)
                             ccGGCAAATACTCCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                 <  2:9066907/140‑1 (MQ=255)
                                  aaaTCCTGCACCGCCGCGCCGGACCAAAATGTATGCTTAAGGTTGGTTTCCCCCCACTCCCTGTGGC‑CGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                                                            <  1:9402301/140‑1 (MQ=255)
                                  aaaGACTCCCCCGACGCGACGCACGAAAATGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                                                            <  2:4439760/140‑1 (MQ=37)
                                      aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGGGCGCCCACACCCAAACCCACACCCCCACCGGAggggggggcgag                                                                                                        >  1:6736191/1‑134 (MQ=21)
                                      aCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGGCCGCACCCAAACCCCAGCCCCCAAggggggggggggcgcg                                                                                                        >  1:7958699/1‑134 (MQ=21)
                                          cACCGACGCGACGTACTAAAATTTATTCGTATGGTTGTTTTTAACCCTCTCCGTGTGGG‑TGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                                                    <  1:1481904/140‑1 (MQ=37)
                                          cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCACACCCAAAACCACAACCACAAAGGAGAGGGGGccagtgtca                                                                                                    >  1:9180942/1‑133 (MQ=25)
                                          cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCCCACCCCAAACCACACCCACCACGGCGAGGGGGcccagcccg                                                                                                    >  2:1233084/1‑133 (MQ=21)
                                              gacgcgtcgGACGACAAAGTATTCGTAAGGTTGTTTTACTCCCTCTCCCTGTGGG‑AGAGCGCCGGGTTGAGGGCATCACCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑caca                                                                                               <  2:10455978/140‑1 (MQ=38)
                                              gacgcgaggGACTAAAATTTATTCTTATGGTGGTTTTTCCCCCTCGCCATGTGGT‑TGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑caca                                                                                               <  1:8322017/140‑1 (MQ=25)
                                               acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCACACCCAAAACCACGCCCACAAGGGAGAGGGGACCGATACAG‑CACaa                                                                                              >  1:4011316/1‑140 (MQ=25)
                                               acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCACACCCAAACCCACTACCTCAAGGGAGTGGGGACCGATAGAG‑CACaa                                                                                              >  2:5778719/1‑140 (MQ=37)
                                               acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACACCCGAACCCACACCCACAAGGGAGAGGGGCCCGAACGAG‑cccca                                                                                              >  2:8035628/1‑136 (MQ=25)
                                                cgcgacgTAAGAAAATTTTTTCTTAGGGTTGTTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACaaa                                                                                             <  1:1530045/140‑1 (MQ=37)
                                                cgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCGCACCCTAACCCACTCCCACAAGGGGGAGGGGACCGATGGGG‑CCCaaa                                                                                             >  2:8153386/1‑140 (MQ=25)
                                                   gacggacgAAAACTTATGCGTACGGTGGGTTTACTCCCTCTCCTTGTGGG‑AGAGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCCCCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAAttt                                                                                          <  2:2412283/140‑1 (MQ=37)
                                                    acggactAAAAAGTATTCGTATGTTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAAtttt                                                                                         <  2:8916082/140‑1 (MQ=255)
                                                    acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGACCCACACCCAAAACCACACCCACCAGGGAGAGGGGGCCGAGAGAG‑CCCAAAattt                                                                                         >  2:29832/1‑140 (MQ=21)
                                                    acggacgAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGGTCACCCGCACCCGAACCCACTCCCGGGAGGGGGGGGGGGGCGGTGGGG‑CACAAAtttg                                                                                         >  1:10590127/1‑139 (MQ=21)
                                                      ggacgAAAATGTATTGGTAAGGTGGGTTTTCGCCCTCTCCCTGGGGT‑TGCGGGCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTga                                                                                       <  2:4087631/140‑1 (MQ=37)
                                                       gactAAAATTTATTCTTATGTTTGTTTTTCTCCATCGCCCTGTGGG‑TGTGGGCCGGGGTCTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgag                                                                                      <  1:8422513/140‑1 (MQ=37)
                                                         cgAAAATGTATTCGTAAGGTTGTTTTTCTCCATATCCGTGTGGG‑AGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                    <  2:2425812/140‑1 (MQ=39)
                                                         cgAAAAATTATTCGTATGGTGGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                    <  1:2958407/140‑1 (MQ=255)
                                                         cgAAAAAGTATTCGTAAGTTTGTTTTACTCCCTCTCCCTGTGGG‑TGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                    <  1:2958696/140‑1 (MQ=39)
                                                              aatttttCGTCCGGTTGGTTTTCCCCCTCCCCCTGTGGG‑TGTGGCCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtc                                                                               <  1:1171527/135‑1 (MQ=37)
                                                              aaGTATTCGTAAGTTGGGTTTTCTCCCTCTCCCTGTGGG‑AGTGGCCCGGGGTGAGTGCATCAGCGCGCACGTTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGTACCGATCGAG‑CACAAATTGTGAGAGATAtc                                                                               <  2:6677123/140‑1 (MQ=38)
                                                                  aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACCCCCCAACCCACCCCCCAAAGGGAGAGGGGCCCGCAAGCG‑CCCAAAATTTTTGAGatataatat                                                                           >  2:5285518/1‑135 (MQ=12)
                                                                   ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCCAAAACACACCCACCAGGGAGCGGGGACCCAACGAG‑CACAAAAATTGAGAGataaataaat                                                                          >  1:4033458/1‑133 (MQ=21)
                                                                    tCGTATGGGTGTTTTTCCCCCACTCCC‑GTGGGTTGTGGGCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                                                         <  1:6078579/140‑1 (MQ=25)
                                                                      gaaaGGTTGGTTATCTCCCTCGCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTg                                                                       <  2:11031402/138‑1 (MQ=38)
                                                                         tggtgggttTTCCCCCTCACCGTGGGGG‑AGGGGGGGGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCt                                                                    <  1:1233230/139‑1 (MQ=37)
                                                                            ttggttTTCTCCCACTCCCTGTGGG‑TGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                                                 <  1:1868059/140‑1 (MQ=255)
                                                                            ctggtcTCCTCCCGCTCCCTGTGGG‑CGAGGGCCGGGGTGCGGGCCTCACCGCGCACGTACACCCTCCCCCTACCCCTCTCCCTCAAGGGAGCGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                                                 <  2:9666206/139‑1 (MQ=255)
                                                                                 tttCTCCCTCTCCGTGGGGG‑TGTGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                                                            <  1:4131939/140‑1 (MQ=38)
                                                                                 tatctccctctcccTGTGGG‑AGTGGGCCGGGGTGGGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                                                            <  1:11011267/138‑1 (MQ=255)
                                                                                   tctccctctcccTGTGGG‑AGAGGGCCGGGGTGAGGGCATCATCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGTGACCGATCTAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                                          <  2:7267455/140‑1 (MQ=255)
                                                                                   tctccctcgcccGGGGGG‑AGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                                          <  2:5151742/140‑1 (MQ=255)
                                                                                                 tgGG‑AGAGGGCCGGGGTGAGGGCATCAGCGGGCACGTTGACCCGAAACCCAAACCAAAACCACCATGGAGTGGGGTCCCATCGAGCCACAAATTTT‑TGAGTTATATTATTATTTGTTGTAAAAGAATAAAAGAAAATAgg                                            >  2:3175787/1‑140 (MQ=255)
                                                                                                      agagGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCACACCCAAAACCACTCCCACCAGGGCGAGGGGACCCATCGAG‑CACAAATTTTGAGAGATATCATATTATGTATTTTAAAAGTAGAAAAGAAAAAAGGGcgt                                        >  2:10918227/1‑138 (MQ=255)
                                                                                                       gagGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt                                       <  1:941847/140‑1 (MQ=255)
                                                                                                         gggCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                                     <  1:6323005/140‑1 (MQ=255)
                                                                                                          ggCCGGGGTGAGGGCATCAGCGCGCACGTTCGCCCCCAACCTAAACCACACCCTCAAGGGCGAGGGGACCGGTCCAG‑CACAAAATTTGAGTGATATATTATTGAGTAGTGTGAAAGAAGAACAGAACAGAGGGCTTTTgg                                    >  2:6538715/1‑139 (MQ=255)
                                                                                                           gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACACCCACCAGGGGGAGGGGGCCGATAGAG‑CACAAAATTTGTGAGATATCATAATAAGTGTTTTTAAAGAAGAACAGAAAATACGGCGTTTtcg                                   >  2:978431/1‑140 (MQ=255)
                                                                                                            ccGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                  <  1:4947396/140‑1 (MQ=255)
                                                                                                            ccGGGGTGAGGGCATCAGCGCGCAGGTGGACCCACACCCTAACCCACACCCACAAGGGAGTGGGGACCCATCGAG‑CCCCACATTTGAGAGCTATATTAATCTGTCTTTGAACCGAAGGACAGACAAAACGGCGTTTgcgc                                  >  2:11179659/1‑140 (MQ=255)
                                                                                                            ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                  <  1:8526114/140‑1 (MQ=255)
                                                                                                            ccGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACACCCCCAAGGGAGAGGGGACCCATAGCG‑CACAAATTTTGAGAGAAAAAATATTATGTCATGTAACAAAACCACACCAAAAAGGGCGTTTgcgc                                  >  2:10683271/1‑140 (MQ=255)
                                                                                                            ccGGGGTGAGGGCATCAGCGCGCACGTGGACCCACACCCTAAACCACAACCACAAGGGAGTGGGGGCCCATCGAG‑CCCAAATTTTGTGTGATATATAATTCTGTCATTTTAAAGAAGAACAGCAAATCGGGCGTTTgggc                                  >  2:1815268/1‑140 (MQ=255)
                                                                                                               gggTGAGGGCATCAGCGCGCACGTTGACCCTCACCCGAACCCACTCCCACCAGGGAGAGGGGACCCATAGAG‑CACAAAATTTTAGAGATATATTAATATTTCTTGTAAAAGAAGAAACCAAAAAAGGGCTTTTTCGCCTg                               >  2:1389233/1‑140 (MQ=255)
                                                                                                                 gTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATTTCTTCTTCTTTCTTGTAACATAAGAACAGAAAATCTGGCTTTTGCGCCTGaa                             <  2:9095873/140‑1 (MQ=255)
                                                                                                                      gccctccgcgcgcACGCCCCCCCTCACACACACCCTCGCCCTCCAGCGAGAGGCGCCCGATCGCG‑CACAAATTTTGAGAGCTATCTTCTTCTGTCATGTAACAGAAGAACAGACCATCGGGCTTTTGCGCCTGAATATTg                        <  2:6959803/133‑1 (MQ=255)
                                                                                                                        cATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg                      <  2:5544432/140‑1 (MQ=255)
                                                                                                                         ctCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTACTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg                     <  2:6119866/139‑1 (MQ=255)
                                                                                                                         aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg                     <  2:9450594/140‑1 (MQ=255)
                                                                                                                                  cACGTTCACCCTCACCCTACCCCTCTCCCTCACGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACCGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc            <  1:6990319/140‑1 (MQ=255)
                                                                                                                                    cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt          <  1:11391189/140‑1 (MQ=255)
                                                                                                                                      ttCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATgg        <  2:6736191/140‑1 (MQ=255)
                                                                                                                                        caccctcaccctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGAGATCTTCTTCTGTCTTGTAACCGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGcc      <  1:7908938/140‑1 (MQ=255)
                                                                                                                                             |                                                                                                                                           
TTATCCCAAACA‑TGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGG‑AGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGA  >  NZ_CP025268/164293‑164570

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑