Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP025268164,4440TA25.0% 268.8 / 31.6 123intergenic (+79/‑117)hrpB/mrcBATP‑dependent helicase HrpB/bifunctional glycosyl transferase/transpeptidase
Reads supporting (aligned to +/- strand):  ref base T (11/79);  new base A (30/0);  total (43/80)
Fisher's exact test for biased strand distribution p-value = 1.86e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 153 total aligned reads displayed.

GTCTGGCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCC  >  NZ_CP025268/164306‑164583
                                                                                                                                           |                                                                                                                                            
gacTGGCCGTACCCCCCGCCAAATA‑CTGCACCTACGCTACGTACTAAAAATTATTCTAACGGTTGTTTTTCTCCCTCCCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  1:2880229/138‑1 (MQ=21)
gTCTGGCCGGCCGACCCGCCAAATA‑CTGCACCGACGCGACGGACTAAAAAGTATTCGTAAGGTTGTTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  1:10536690/140‑1 (MQ=38)
gTCTGGCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  2:10706072/140‑1 (MQ=255)
gTCGGGCCGGACGCCCCGCCACATA‑CTGCACCTACGCGACGGCCGAAAAATTAGTCGAATGTTTGGTTTGCCCCCTCTCCCTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                                             <  1:7688529/140‑1 (MQ=25)
 tCTGGCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCcact                                                                                                                                            >  2:10344839/1‑140 (MQ=255)
 tCTGGCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCTCCCACACCCGAAACcact                                                                                                                                            >  1:3918838/1‑140 (MQ=37)
 tCTGCCCGGACGCCCCGCCAAATA‑CTGCACCGACGCGACGCACGAAAAAGTATTCGTAAGGTTGGTTTGCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCctct                                                                                                                                            <  2:6379155/140‑1 (MQ=255)
  gtGGCCGGACTCCGCGGCATATA‑GTGCACCGAGGCGACGTACTAAAATGTATTCGTAAGGTTGGTTTTCTCCCGATCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCtctc                                                                                                                                           <  2:8481113/139‑1 (MQ=25)
  cTGGCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGTTCACCCACACCCTAACCctcac                                                                                                                                           >  2:3732201/1‑138 (MQ=255)
  cTGGCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCTCACCCAAACCCtctc                                                                                                                                           >  2:11034152/1‑140 (MQ=255)
   tGGCCGGAGGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATGCGTAAGGTTGGTTTTCTCCCACTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                                                                          <  2:9591777/140‑1 (MQ=255)
   tGGCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTATGGTTGGTTTTCTCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTcc                                                                                                                                          <  2:9098020/140‑1 (MQ=255)
     gCCGGACGCCCCGGCAAAAA‑CGCCACCGACGCGCCGGACTAAACAGTATGCGTACGGGGGTTTGTCTCCCACGCCCGGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTACCCCTCTCCCt                                                                                                                                        <  2:10794729/140‑1 (MQ=21)
     gCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCGTGCACCCGCACCCGAACCCACAccca                                                                                                                                        >  2:2762805/1‑139 (MQ=37)
     gCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGGGGCCCGCACCCAAACCCACAccca                                                                                                                                        >  1:8916082/1‑139 (MQ=37)
         gccgacCCTGAAAATC‑CTCCACCGCGGCGAGGTACTAAAATTTATTCTTTTGGTTGGTTTTCTCCCTCTCCCGGTGGTAGTGGGCGGGGGTGTGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                                                    <  1:5544691/138‑1 (MQ=255)
         gacgccCCGGCAAATA‑CGGCACCGCCGCGCCGGACGAAAAAGTATTCGAACGGTTGGTTTTCTCCCTCCCCCTGTGGGAGAGGGCCGGGGGGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                                                    <  2:3021099/140‑1 (MQ=38)
           cgacCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCCCACCCACACCCCCACggg                                                                                                                                  >  1:11267880/1‑140 (MQ=25)
           cctcccGGCAAAAACCTGCACCCAGGCCCCGCCCAAAAATTTTTTCGTATGTTTGTTTTACCCCCTCCCCCGGTGGTTGTGGGCGGGGGTGTGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAgg                                                                                                                                   <  1:713127/137‑1 (MQ=255)
            gacCCGTAAAATC‑CTCCACCGACGCGACGTAATAAAATTTATTCGAAAGGTTGGTTTACCCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga                                                                                                                                 <  1:1762052/140‑1 (MQ=255)
             ccccGGCAAACA‑CCCCCCCGACGCGACGGACGAAAAAGTATTCGTACGGTTGGTTTTCCCCCGCGCCCGGGGGGCGCGGGCCGGGGTGCGGGCAGCAGCGCGCACGTTCACCCTCACCCTCACCCTCTCCCTCAAGGgag                                                                                                                                <  2:8923856/139‑1 (MQ=255)
             acCCGCAAAATA‑CTGCCCCGACGCGACGGACTAAAATTTATTCGTATGGTGGGTTTTCTCCCTCGCCCTGTGGGTGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGgag                                                                                                                                <  1:10312989/140‑1 (MQ=255)
               ccGGCAAATA‑CTGGACCGACGCGACGGACTAAAAATTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                              <  2:1852130/140‑1 (MQ=255)
               ccGGCAAATA‑CTGCACCGACGCGACGGACGAAAAATTATTCGTAAGTTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                              <  2:1591420/140‑1 (MQ=255)
               ccGGCAAATA‑CTCCCCCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                                              <  2:9066907/140‑1 (MQ=255)
                    aaaGA‑CTCCCCCGACGCGACGCACGAAAATGTATGCGTAAGGTTGGTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCCCCCTAACCCTCTCCCTCAAGGGAGAGGGGAc                                                                                                                         <  2:4439760/140‑1 (MQ=37)
                        a‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGGCCGCACCCAAACCCCAGCCCCCAAggggggggggggcgcg                                                                                                                     >  1:7958699/1‑134 (MQ=21)
                             cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGCCCCACACCCAAAACCAAAACCACAAGGGGGAGGGGGCCGATagag                                                                                                                 >  2:1233230/1‑140 (MQ=21)
                             cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCCCACCCCAAACCACACCCACCACGGCGAGGGGGcccagcccg                                                                                                                 >  2:1233084/1‑133 (MQ=21)
                             cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTGTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCGCAGCGCGCCCGGGCACCCGCACCCGATCCCTCTCCCTCACGGGCGCGGGGGCCgctagcg                                                                                                                 >  2:8836434/1‑134 (MQ=25)
                                 gacgcgtcgGACGACAAAGTATTCGTAAGGTTGTTTTACTCCCTCTCCCTGTGGGAGAGCGCCGGGTTGAGGGCATCACCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑caca                                                                                                            <  2:10455978/140‑1 (MQ=38)
                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTGCACCCACACCCAAAACCACGCCCACAAGGGAGAGGGGACCGATACAG‑CACaa                                                                                                           >  1:4011316/1‑140 (MQ=25)
                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCACACCCAAACCCACTACCTCAAGGGAGTGGGGACCGATAGAG‑CACaa                                                                                                           >  2:5778719/1‑140 (MQ=37)
                                  acgcgacgGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTCACCCACACCCGAACCCACACCCACAAGGGAGAGGGGCCCGAACGAG‑cccca                                                                                                           >  2:8035628/1‑136 (MQ=25)
                                   cgcgacgTAAGAAAATTTTTTCTTAGGGTTGTTTTTCTCCCTCTCCCTGTGGGAGTGGGCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACaaa                                                                                                          <  1:1530045/140‑1 (MQ=37)
                                      gacggacgAAAACTTATGCGTACGGTGGGTTTACTCCCTCTCCTTGTGGGAGAGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCCCCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAAttt                                                                                                       <  2:2412283/140‑1 (MQ=37)
                                         ggacgAAAATGTATTGGTAAGGTGGGTTTTCGCCCTCTCCCTGGGGTTGCGGGCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTga                                                                                                    <  2:4087631/140‑1 (MQ=37)
                                          gactAAAATTTATTCTTATGTTTGTTTTTCTCCATCGCCCTGTGGGTGTGGGCCGGGGTCTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgag                                                                                                   <  1:8422513/140‑1 (MQ=37)
                                            cgAAAATGTATTCGTAAGGTTGTTTTTCTCCATATCCGTGTGGGAGAGGGCCGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                                 <  2:2425812/140‑1 (MQ=39)
                                            cgAAAAATTATTCGTATGGTGGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                                 <  1:2958407/140‑1 (MQ=255)
                                            cgAAAAAGTATTCGTAAGTTTGTTTTACTCCCTCTCCCTGTGGGTGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTgagag                                                                                                 <  1:2958696/140‑1 (MQ=39)
                                              aacaaGTATTCGTAAGGTTGTTTTTCGCCCGCTCCCTGGGGGAGTGGGCCGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGat                                                                                               <  1:8390543/137‑1 (MQ=38)
                                                aaaGTATTCGTATGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGatat                                                                                             <  2:1845747/140‑1 (MQ=255)
                                                 aatttttCGTCCGGTTGGTTTTCCCCCTCCCCCTGTGGGTGTGGCCGGGGGTGTGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtc                                                                                            <  1:1171527/135‑1 (MQ=37)
                                                 aaGTATTCGTAAGTTGGGTTTTCTCCCTCTCCCTGTGGGAGTGGCCCGGGGTGAGTGCATCAGCGCGCACGTTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGTACCGATCGAG‑CACAAATTGTGAGAGATAtc                                                                                            <  2:6677123/140‑1 (MQ=38)
                                                 aaGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtc                                                                                            <  2:3993761/140‑1 (MQ=255)
                                                   gttttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGACTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtctt                                                                                          <  1:7576052/137‑1 (MQ=255)
                                                    tATTCTTATGGTTGTTTTTCTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttc                                                                                         <  1:10344839/140‑1 (MQ=255)
                                                     aTTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCACCCCCCAACCCACCCCCCAAAGGGAGAGGGGCCCGCAAGCG‑CCCAAAATTTTTGAGatataatat                                                                                        >  2:5285518/1‑135 (MQ=12)
                                                      ttCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCCAAAACACACCCACCAGGGAGCGGGGACCCAACGAG‑CACAAAAATTGAGAGataaataaat                                                                                       >  1:4033458/1‑133 (MQ=21)
                                                       tCGTAAGGTGGGTTTACTCCCTCGCCGTGTGGGAGAGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                                                                      <  1:8814548/140‑1 (MQ=39)
                                                       gcttttggttggttTTCTCCCTCTCCCTGGGGTTGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATAtcttcttc                                                                                      <  1:6472678/134‑1 (MQ=38)
                                                         gaaaGGTTGGTTATCTCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTg                                                                                    <  2:11031402/138‑1 (MQ=38)
                                                            tggtgggttTTCCCCCTCACCGTGGGGGAGGGGGGGGGGGGGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCt                                                                                 <  1:1233230/139‑1 (MQ=37)
                                                               ttggttTTCTCCCACTCCCTGTGGGTGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                                                              <  1:1868059/140‑1 (MQ=255)
                                                               ctggtcTCCTCCCGCTCCCTGTGGGCGAGGGCCGGGGTGCGGGCCTCACCGCGCACGTACACCCTCCCCCTACCCCTCTCCCTCAAGGGAGCGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGt                                                                              <  2:9666206/139‑1 (MQ=255)
                                                                    tttCTCCCTCTCCGTGGGGGTGTGGGCGGGGGTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                                                                         <  1:4131939/140‑1 (MQ=38)
                                                                    tttCTCCCTCTCCGGGGGGGAGTGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                                                                         <  1:7912655/140‑1 (MQ=39)
                                                                    tttCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                                                                         <  1:10693297/140‑1 (MQ=255)
                                                                    tttCTCCCTCTCCCTGTAGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACag                                                                         <  1:2656325/140‑1 (MQ=255)
                                                                      tctccctctcccTGTGGGAGAGGGCCGGGGTGAGGGCATCATCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGTGACCGATCTAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                                                       <  2:7267455/140‑1 (MQ=255)
                                                                      tctccctcgcccGGGGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACagaa                                                                       <  2:5151742/140‑1 (MQ=255)
                                                                      tcccccgctccctGTGTGAGCGGGCCGGGGTTAGGTCCTCAGCGCGCACGTTCACACTCACACTAACCCTCTCCCTAAAGGGAGAGGGGACCGATCGCG‑CACAAATTTTGATAGATATCTTCTTCTGTAGTGTAACagaa                                                                       <  1:682513/137‑1 (MQ=255)
                                                                                    tgGGAGAGGGCCGGGGTGAGGGCATCAGCGGGCACGTTGACCCGAAACCCAAACCAAAACCACCATGGAGTGGGGTCCCATCGAGCCACAAATTTT‑TGAGTTATATTATTATTTGTTGTAAAAGAATAAAAGAAAATAgg                                                         >  2:3175787/1‑140 (MQ=255)
                                                                                         gagGGCGGGGTTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt                                                    <  1:1275572/140‑1 (MQ=255)
                                                                                         gagGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt                                                    <  1:941847/140‑1 (MQ=255)
                                                                                         gagGGCCGGGGTGCGGGCATCAGCGCGCACGTACACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCttt                                                    <  1:6018609/140‑1 (MQ=255)
                                                                                           gggCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTg                                                  <  1:6323005/140‑1 (MQ=255)
                                                                                            ggCCGGGGTGAGGGCATCAGCGCGCACGTTCGCCCCCAACCTAAACCACACCCTCAAGGGCGAGGGGACCGGTCCAG‑CACAAAATTTGAGTGATATATTATTGAGTAGTGTGAAAGAAGAACAGAACAGAGGGCTTTTgg                                                 >  2:6538715/1‑139 (MQ=255)
                                                                                            ggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGCACCCAAAACCACGCCCACAAGGGAGAGGGGCCCGCTCGAG‑CCCAAAATTTTAGAGAAATAATATTATGTAATGTAAAAGAAAAACAGAAAAAAGGGCtttttc                                                 >  2:2902334/1‑138 (MQ=255)
                                                                                             gCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCACACCCACCAGGGGGAGGGGGCCGATAGAG‑CACAAAATTTGTGAGATATCATAATAAGTGTTTTTAAAGAAGAACAGAAAATACGGCGTTTtcg                                                >  2:978431/1‑140 (MQ=255)
                                                                                              ccGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTgcgc                                               <  1:4947396/140‑1 (MQ=255)
                                                                                                        gccctccgcgcgcACGCCCCCCCTCACACACACCCTCGCCCTCCAGCGAGAGGCGCCCGATCGCG‑CACAAATTTTGAGAGCTATCTTCTTCTGTCATGTAACAGAAGAACAGACCATCGGGCTTTTGCGCCTGAATATTg                                     <  2:6959803/133‑1 (MQ=255)
                                                                                                          cATCAGCGCGCACGTGCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGCTCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg                                   <  2:1414980/140‑1 (MQ=255)
                                                                                                           ctCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTACTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg                                  <  2:6119866/139‑1 (MQ=255)
                                                                                                           aTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCgg                                  <  2:9450594/140‑1 (MQ=255)
                                                                                                                    cACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGc                         <  1:9402971/140‑1 (MQ=255)
                                                                                                                     ccGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCa                        <  1:5828662/139‑1 (MQ=255)
                                                                                                                      cGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCAt                       <  1:11391189/140‑1 (MQ=255)
                                                                                                                              ctcaccctAACCCGCTCCCTCAAGGGAGAGGGGACCGCTCGAG‑CACAAATTTTGAGAGAGAACTTCTTCTGTCTTGTAACAGAAGAACAGCAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGa               <  2:6973897/140‑1 (MQ=255)
                                                                                                                               gcacccgAACCCACTCCCACAAGGGAGAGGGGACCCATCGAG‑CACAAATTTTTAGAGCTATCTTATTCTGTGTTTTAACACAAGAACAGAAAATCGGGCGTTTGCGCCGGAATATTGCGGACTAAAAGCATGGGCgggta              >  2:7384389/2‑138 (MQ=255)
                                                                                                                                caccctAACCCTCTCCCTCTAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGAGATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAAt             <  2:4199341/140‑1 (MQ=255)
                                                                                                                                 accctAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATg            <  2:5191809/140‑1 (MQ=255)
                                                                                                                                   ccaaaCCCACTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGTGAGATATATTCTTATGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGAc          >  1:4929355/4‑140 (MQ=255)
                                                                                                                                     taccccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcg        <  2:7847150/137‑1 (MQ=255)
                                                                                                                                     tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcg        <  1:9247136/140‑1 (MQ=255)
                                                                                                                                     tAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcg        <  1:11207567/140‑1 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg      >  1:7688358/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg      >  1:10945700/1‑140 (MQ=255)
                                                                                                                                       aCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTGGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACcgcg      >  2:6076379/1‑140 (MQ=255)
                                                                                                                                         ccTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAg    <  1:9337083/140‑1 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGCG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:9045057/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:10557822/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:10642566/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:9829124/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:5495209/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  2:5428437/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  2:5607347/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGcc  >  1:8828977/1‑140 (MQ=255)
                                                                                                                                           tctcCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATGTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAACATCGGGCTTTTGCGCCTGAATATTGCGGAGAACAAGCATGGCCGGGAATGACCGCGAGcg  >  1:10887805/1‑139 (MQ=255)
                                                                                                                                           |                                                                                                                                            
GTCTGGCCGGACGACCCGGCAAATA‑CTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGGTTTTCTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAG‑CACAAATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCATGGCCGGGAATGACCGCGAGCC  >  NZ_CP025268/164306‑164583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑