BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP025268	498,064	T→C	R320R (CGT→CGC) 320 (TAA→TAG)	hemH → aes ←	ferrochelatase acetyl esterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP025268	498,064	0	T	C	99.8%	1821.5 / NA	527	R320R (CGT→CGC) 320 (TAA→TAG)	hemH aes	ferrochelatase acetyl esterase
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (259/266); minor base A (0/1); total (260/267)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 527 total aligned reads displayed.

GAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTA  >  NZ_CP025268/497927‑498200

gagaCGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCGTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTa                                                                                                                                        <  1:10618653/140‑1 (MQ=255)

   aCGCTGGAAGAGATTGCCGAGCAACATCGTGAGGTCGTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAg                                                                                                                                     <  2:9503866/140‑1 (MQ=255)

    cGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGc                                                                                                                                    >  1:7499881/1‑140 (MQ=255)

      cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAg                                                                                                                                     >  1:7767450/1‑137 (MQ=255)

      cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTg                                                                                                                                  >  1:7412920/1‑140 (MQ=255)

      cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTg                                                                                                                                  >  1:4618274/1‑140 (MQ=255)

      cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTg                                                                                                                                  >  1:5009582/1‑140 (MQ=255)

      cTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTg                                                                                                                                  >  1:9104367/1‑140 (MQ=255)

        ggAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAg                                                                                                                                <  2:466355/140‑1 (MQ=255)

        ggAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAg                                                                                                                                <  1:298763/140‑1 (MQ=255)

        ggAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGAGGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAg                                                                                                                                <  1:8584594/140‑1 (MQ=255)

        cgaccagagTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAACATATGAATATATTCCGGCGCTTAATGCCACGACGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAg                                                                                                                                <  1:8086384/135‑1 (MQ=255)

          aaGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCg                                                                                                                              <  2:190390/140‑1 (MQ=255)

               aTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTaaa                                                                                                                         >  2:6907352/1‑140 (MQ=255)

               aTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTaaa                                                                                                                         >  1:9338947/1‑140 (MQ=255)

               aTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTaaa                                                                                                                         >  2:864396/1‑140 (MQ=255)

               aTTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTaaa                                                                                                                         >  2:1005077/1‑140 (MQ=255)

                   ccGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAAc                                                                                                                     >  1:3630056/1‑140 (MQ=255)

                   ccGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAAc                                                                                                                     >  2:4264773/1‑140 (MQ=255)

                   ccGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAAc                                                                                                                     >  1:2378721/1‑140 (MQ=255)

                   ccGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAAc                                                                                                                     >  2:8508906/1‑140 (MQ=255)

                      aGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGa                                                                                                                  >  1:1282448/1‑140 (MQ=255)

                         aaaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACCTATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAgcg                                                                                                               <  1:8769606/140‑1 (MQ=255)

                         aaaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAgcg                                                                                                               >  1:2628557/1‑140 (MQ=255)

                         aaaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAgcg                                                                                                               <  2:5663859/140‑1 (MQ=255)

                            aCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATAGTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCg                                                                                                            <  2:4062225/140‑1 (MQ=255)

                                tGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTCAAGACCTGAGCGCCGTcgc                                                                                                        >  2:10548477/1‑140 (MQ=255)

                                tGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTcgc                                                                                                        >  1:6338040/1‑140 (MQ=255)

                                 gAGCTCTTCCTCGGTGCCGGCGGGAAAAAATATGAAGATACTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTcgcg                                                                                                       <  1:10870322/140‑1 (MQ=255)

                                     tCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAaga                                                                                                   <  2:7172239/140‑1 (MQ=255)

                                     tCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGGTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAaga                                                                                                   <  2:2967249/140‑1 (MQ=255)

                                            cGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtcgt                                                                                            <  1:6679940/140‑1 (MQ=255)

                                              gTGCCGGCGGGAAACAATATGCATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTGGCCGCGTATCGCTAAAGCTGAGCGGTCAAGACCTGAGCGCCGTCGCGAAGAGCCtcgtcg                                                                                          >  2:11178390/1‑140 (MQ=255)

                                              gTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCtcgtcg                                                                                          >  1:5662327/1‑140 (MQ=255)

                                               tGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg                                                                                         >  2:1463957/1‑140 (MQ=255)

                                               tGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcgg                                                                                         >  2:11519320/1‑140 (MQ=255)

                                               tGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCAACGACGGAACATATCGAAATGATGGCTAAACTTGTTGCCGCGTATCGCTCAAGCTGAGCGATAAAGAACTTAGCGCAGTCGCGAAGAGCCTCGTcgg                                                                                         >  2:9754394/1‑140 (MQ=255)

                                                 ccGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGACCTGAGCGCCGTCGCGAAGAGCCTCGTcggcg                                                                                       >  1:11460953/1‑140 (MQ=255)

                                                 ccGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTcggcg                                                                                       >  2:3829373/1‑140 (MQ=255)

                                                    gcggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGtt                                                                                    >  1:4836820/1‑140 (MQ=255)

                                                    gcggGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGtt                                                                                    >  1:9298771/1‑140 (MQ=255)

                                                         aaaaaaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                               <  1:8912559/140‑1 (MQ=255)

                                                         aaaaaaTATGAATAGATTCCGGCGCTTAATGCCACGCCGGAACAGATCGAAATGATGGCTAATCTAGGTGCCGCGTCTCGCTACAGCTGAGCGGTAAAGAACGGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTtcat                                                                               <  2:5499301/140‑1 (MQ=255)

                                                             aaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCatcatc                                                                           <  2:3467539/140‑1 (MQ=255)

                                                              atatGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGCTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATcc                                                                          <  1:1030408/140‑1 (MQ=255)

                                                              atatGAATATATTCCGGCGCTTAATGCCACGCCGGAACAGATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATcc                                                                          <  2:4407050/140‑1 (MQ=255)

                                                                          tCCGGCGCTTAATGCCACGCCGGAACAGATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCa                                                              <  2:4895573/140‑1 (MQ=255)

                                                                             ggCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCaaaa                                                           >  1:3529642/1‑140 (MQ=255)

                                                                               cgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAg                                                         >  2:2362329/1‑140 (MQ=255)

                                                                                 cTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGc                                                       >  2:6049639/1‑140 (MQ=255)

                                                                                 cTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGc                                                       >  1:6208446/1‑140 (MQ=255)

                                                                                 cTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGc                                                       >  1:5514459/1‑140 (MQ=255)

                                                                                  ttAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCg                                                      <  1:3375366/140‑1 (MQ=255)

                                                                                  ttAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGAGTATCGCTAAAGCTGAGCGGTAAAGAACAGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCg                                                      <  2:7301244/140‑1 (MQ=255)

                                                                                    aaTGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTg                                                    >  2:2219174/1‑140 (MQ=255)

                                                                                       gCCACGCCGGAACCTCTCGAACTGATGGCTAATCTTGTTGCCGAGTATCGCTAAAGCTGAGCGGTAAAGAACTGCGCGCCGTCGCGAAGACCCTCGTCGGCGGTTTTCATCCTCCGTGAATAATGCAAACAGGCGTGCAg                                                 <  2:2782654/140‑1 (MQ=255)

                                                                                       gCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAg                                                 <  2:704601/140‑1 (MQ=255)

                                                                                       gCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAg                                                 <  1:4029635/140‑1 (MQ=255)

                                                                                       gCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAg                                                 <  2:6462380/140‑1 (MQ=255)

                                                                                       gCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAg                                                 <  1:7662284/140‑1 (MQ=255)

                                                                                       gCCACGCCGGAACAGATCGAACTGCTGGCTAATCTTGTTGCCGCGTATCGCTAAAGCGGAGCGGTAAAGAACTGAGCGCCGTCGCGCAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAACAGGCGTGCAg                                                 <  2:7974020/140‑1 (MQ=255)

                                                                                       cccACGCCGGAACATATCGAAATGATGGCTAATCTTGGTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAg                                                 <  1:9828190/139‑1 (MQ=255)

                                                                                         cACGCCGGAACATATCGAAATGATGGCTACTCTTGTTGCCGCGTATCGCTCAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCg                                               <  1:389132/140‑1 (MQ=255)

                                                                                         cACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCg                                               >  1:1124338/1‑140 (MQ=255)

                                                                                         cACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCg                                               <  1:2219174/140‑1 (MQ=255)

                                                                                           cGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTg                                             >  1:6003849/1‑140 (MQ=255)

                                                                                           cGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTg                                             <  1:1750082/140‑1 (MQ=255)

                                                                                           cGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTg                                             >  2:10863998/1‑140 (MQ=255)

                                                                                           cGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTg                                             >  1:7956873/1‑140 (MQ=255)

                                                                                            gCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGc                                            <  2:895511/140‑1 (MQ=255)

                                                                                                 aaCATATCGAAATGATGGCTACTCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTggg                                       <  1:1906321/140‑1 (MQ=255)

                                                                                                  aCATATCGAAATGCTGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATTCAAAAAGGCGTTCAGCGTGCCTGGGt                                      <  2:1611045/140‑1 (MQ=255)

                                                                                                      atagAAATGTTGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAGCTGAGCGCCGACGCGCAGAGACTCGTCGGCGGTGTGCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTagag                                  >  2:910952/4‑140 (MQ=255)

                                                                                                      atCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTagag                                  >  1:8184766/1‑140 (MQ=255)

                                                                                                      atCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTagag                                  >  1:1945714/1‑140 (MQ=255)

                                                                                                      atCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGGTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTagag                                  >  2:5896579/1‑140 (MQ=255)

                                                                                                        cGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGtt                                >  2:3310178/1‑140 (MQ=255)

                                                                                                         gAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGttt                               <  1:4375108/140‑1 (MQ=255)

                                                                                                           aaTGATGGCTACTCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCACAAAGGCGTGCAGCGTGCCTGGGTAGAGATTGa                             <  1:1567253/140‑1 (MQ=255)

                                                                                                           aaTGATGGCTACTCTTGTTGCCGCGTATCGCTAAAGCAGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGa                             <  2:8256507/140‑1 (MQ=255)

                                                                                                           aaTGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGa                             <  1:100731/140‑1 (MQ=255)

                                                                                                           aaTGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGa                             <  1:6907924/140‑1 (MQ=255)

                                                                                                               atgGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTc                         >  2:5390193/1‑140 (MQ=255)

                                                                                                                tgGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTg                                             >  2:6293043/1‑119 (MQ=255)

                                                                                                                tgGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                        >  2:6664398/1‑140 (MQ=255)

                                                                                                                tgGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTca                        <  2:2039995/140‑1 (MQ=255)

                                                                                                                tgGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGAGTGCCTGGGTAGAGTTTGAACTct                        >  2:9332573/1‑139 (MQ=255)

                                                                                                                 ggCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTcac                       >  1:6404166/1‑140 (MQ=255)

                                                                                                                 ggCTAATCTGGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGTACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCGTCATCGGTGAATAGTGCAAGTAGGCGTGCTGCGTGCGGGGGTCGAGTTTGTTCTcac                       >  1:1105768/1‑140 (MQ=255)

                                                                                                                   cTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAg                     <  2:5241007/140‑1 (MQ=255)

                                                                                                                      ctCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCCCCGCCGCGAACAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGgc                  <  2:11421709/139‑1 (MQ=255)

                                                                                                                            ttgCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGa            >  2:10392532/1‑140 (MQ=255)

                                                                                                                              gCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATg          <  2:3630056/140‑1 (MQ=255)

                                                                                                                              gCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATg          >  2:3935485/1‑140 (MQ=255)

                                                                                                                              gCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTGTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATg          <  1:5103128/140‑1 (MQ=255)

                                                                                                                                cgcgTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATgcg        <  1:8382032/140‑1 (MQ=255)

                                                                                                                                   gTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGcgccg     >  2:7803738/1‑140 (MQ=255)

                                                                                                                                    tATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGcgccgc    <  2:11111326/140‑1 (MQ=255)

                                                                                                                                     aTCGCTAAAGCTGCGCGGTAAAGTACTGAGCGCCGTCGCGACGAGCCGCGTCGGCGGGTGTCATCAGCCGTGAAGACTGCAAAAAGGCGTGCAGCGTGCCTGGGTCGAGTTTGCACTCAGCGGGCTGCGGTGGCGCCGCt   >  2:7439226/1‑140 (MQ=255)

                                                                                                                                     aTCGCTAAAGCTGAGCGGTAAAGAAGTGAGCGCCGACGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGGAGAGTGTGAACTCACAGGGCTGCTGATGCGCCGCt   >  2:7672851/1‑140 (MQ=255)

GAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGTTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTA  >  NZ_CP025268/497927‑498200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`